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WO2009010661A3 - Reacteur pour la mise en oeuvre d'un procede de culture de tissus osseux - Google Patents

Reacteur pour la mise en oeuvre d'un procede de culture de tissus osseux Download PDF

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Publication number
WO2009010661A3
WO2009010661A3 PCT/FR2008/000846 FR2008000846W WO2009010661A3 WO 2009010661 A3 WO2009010661 A3 WO 2009010661A3 FR 2008000846 W FR2008000846 W FR 2008000846W WO 2009010661 A3 WO2009010661 A3 WO 2009010661A3
Authority
WO
WIPO (PCT)
Prior art keywords
bone
bone tissue
cells
stem cells
tissue culture
Prior art date
Application number
PCT/FR2008/000846
Other languages
English (en)
Other versions
WO2009010661A2 (fr
Inventor
Bertrand David
Herve Petite
Valentin Myrtil
Original Assignee
Univ Paris Diderot Paris 7
Centre Nat Rech Scient
Bertrand David
Herve Petite
Valentin Myrtil
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Paris Diderot Paris 7, Centre Nat Rech Scient, Bertrand David, Herve Petite, Valentin Myrtil filed Critical Univ Paris Diderot Paris 7
Publication of WO2009010661A2 publication Critical patent/WO2009010661A2/fr
Publication of WO2009010661A3 publication Critical patent/WO2009010661A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3821—Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A61L27/3847—Bones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Mechanical Engineering (AREA)
  • Vascular Medicine (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)

Abstract

L'invention concerne un procédé de culture de tissus osseux. Ledit procédé étant du type selon lequel : on fournit des particules d'un matériau compatible avec le tissu osseux; on fournit des cellules souches de cellules osseuses; on ensemence lesdites particules avec lesdites cellules souches de cellules osseuses; on fournit un fluide nutritionnel (31) contenant des éléments nutritionnels destinés à faire proliférer des cellules osseuses; on délivre lesdits éléments nutritionnels auxdites cellules souches de cellules osseuses par l'intermédiaire d'un écoulement dudit fluide nutritionnel (31); et, selon l'invention on maintient lesdites cellules souches de cellules osseuses à l'intérieur dudit écoulement de façon que ledit écoulement exerce des contraintes mécaniques sur lesdites cellules et que lesdites cellules osseuses prolifèrent sur lesdites particules (35) pour former des granules de tissu osseux implantables. On récupère ensuite lesdits granules de tissus osseux implantables pour les transplanter.
PCT/FR2008/000846 2007-06-18 2008-06-18 Reacteur pour la mise en oeuvre d'un procede de culture de tissus osseux WO2009010661A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0704319 2007-06-18
FR0704319A FR2917426B1 (fr) 2007-06-18 2007-06-18 Reacteur pour la mise en oeuvre d'un procede de culture de tissus osseux

Publications (2)

Publication Number Publication Date
WO2009010661A2 WO2009010661A2 (fr) 2009-01-22
WO2009010661A3 true WO2009010661A3 (fr) 2009-04-09

Family

ID=38722643

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2008/000846 WO2009010661A2 (fr) 2007-06-18 2008-06-18 Reacteur pour la mise en oeuvre d'un procede de culture de tissus osseux

Country Status (2)

Country Link
FR (1) FR2917426B1 (fr)
WO (1) WO2009010661A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017316545B2 (en) 2016-08-21 2022-12-08 Adva Biotechnology Ltd. Bioreactor and methods of use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007039600A1 (fr) * 2005-10-04 2007-04-12 Artelis Procede de cultures cellulaires et dispositif pour la mise en oeuvre dudit procede

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007039600A1 (fr) * 2005-10-04 2007-04-12 Artelis Procede de cultures cellulaires et dispositif pour la mise en oeuvre dudit procede

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEN HUANG-CHI ET AL: "Bioreactors for tissue engineering", BIOTECHNOLOGY LETTERS, vol. 28, no. 18, September 2006 (2006-09-01), pages 1415 - 1423, XP002460651, ISSN: 0141-5492 *
HOLTORF HEIDI L ET AL: "Flow perfusion culture of marrow stromal cells seeded on porous biphasic calcium phosphate ceramics", ANNALS OF BIOMEDICAL ENGINEERING, vol. 33, no. 9, September 2005 (2005-09-01), pages 1238 - 1248, XP002460650, ISSN: 0090-6964 *
JANSSEN ET AL: "A perfusion bioreactor system capable of producing clinically relevant volumes of tissue-engineered bone: In vivo bone formation showing proof of concept", BIOMATERIALS, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB, vol. 27, no. 3, January 2006 (2006-01-01), pages 315 - 323, XP005148281, ISSN: 0142-9612 *
VIATEAU VERONIQUE ET AL: "Long-bone critical-size defects treated with tissue-engineered grafts: A study on sheep", JOURNAL OF ORTHOPAEDIC RESEARCH, vol. 25, no. 6, June 2007 (2007-06-01), pages 741 - 749, XP002460649, ISSN: 0736-0266 *

Also Published As

Publication number Publication date
FR2917426B1 (fr) 2013-02-08
FR2917426A1 (fr) 2008-12-19
WO2009010661A2 (fr) 2009-01-22

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