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WO2002055687A3 - Souris transgeniques presentant des disruptions geniques cibles - Google Patents

Souris transgeniques presentant des disruptions geniques cibles Download PDF

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Publication number
WO2002055687A3
WO2002055687A3 PCT/US2001/048262 US0148262W WO02055687A3 WO 2002055687 A3 WO2002055687 A3 WO 2002055687A3 US 0148262 W US0148262 W US 0148262W WO 02055687 A3 WO02055687 A3 WO 02055687A3
Authority
WO
WIPO (PCT)
Prior art keywords
transgenic mice
target gene
containing target
mice containing
gene disruptions
Prior art date
Application number
PCT/US2001/048262
Other languages
English (en)
Other versions
WO2002055687A2 (fr
Inventor
Keith D. Allen
Original Assignee
Deltagen, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deltagen, Inc. filed Critical Deltagen, Inc.
Publication of WO2002055687A2 publication Critical patent/WO2002055687A2/fr
Publication of WO2002055687A3 publication Critical patent/WO2002055687A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Mycology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des animaux transgéniques, de même que des compositions et procédés relatifs à la caractérisation de la fonction génique. L'invention concerne notamment des souris transgéniques comprenant des mutations dans un gène cible. De telles souris transgéniques sont utiles en tant que modèles pour des maladies et pour l'identification d'agents modulant l'expression et la fonction géniques, et en tant que traitements potentiels de diverses pathologies et maladies.
PCT/US2001/048262 2000-12-13 2001-12-13 Souris transgeniques presentant des disruptions geniques cibles WO2002055687A2 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US25620600P 2000-12-13 2000-12-13
US32689201P 2001-10-02 2001-10-02
US60/326,892 2001-10-02
US1554901A 2001-12-11 2001-12-11
US10/015,549 2001-12-11
US60/256,206 2002-02-12

Publications (2)

Publication Number Publication Date
WO2002055687A2 WO2002055687A2 (fr) 2002-07-18
WO2002055687A3 true WO2002055687A3 (fr) 2003-05-22

Family

ID=27360372

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/048262 WO2002055687A2 (fr) 2000-12-13 2001-12-13 Souris transgeniques presentant des disruptions geniques cibles

Country Status (1)

Country Link
WO (1) WO2002055687A2 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999020780A1 (fr) * 1997-10-20 1999-04-29 Roche Diagnostics Gmbh Selection par gene marqueur positif ou negatif lors d'une recombinaison homologue

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999020780A1 (fr) * 1997-10-20 1999-04-29 Roche Diagnostics Gmbh Selection par gene marqueur positif ou negatif lors d'une recombinaison homologue

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CAPECCHI M R: "TARGETED GENE REPLACEMENT", SCIENTIFIC AMERICAN, SCIENTIFIC AMERICAN INC. NEW YORK, US, March 1994 (1994-03-01), pages 52 - 59, XP002940139, ISSN: 0036-8733 *
COHEN-TANNOUDJI M ET AL: "BEYOND 'KNOCK-OUT' MICE: NEW PERSPECTIVES FOR THE PROGRAMMED MODIFICATION OF THE MAMMALIAN GENOME", MOLECULAR HUMAN REPRODUCTION, OXFORD, GB, vol. 4, no. 10, October 1998 (1998-10-01), pages 929 - 938, XP001056181, ISSN: 1360-9947 *
FAUST N, VARAS F, KELLY LM, HECK S, GRAF T.: "Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages.", : BLOOD 2000 JUL 15;96(2):719-26, XP002227856 *
MANSOUR S L ET AL: "DISRUPTION OF THE PROTO-ONCOGENE INT-2 IN MOUSE EMBRYO-DERIVED STEM CELLS: A GENERAL STRATEGY FOR TARGETING MUTATIONS TO NON-SELECTABLE GENES", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 336, 24 November 1988 (1988-11-24), pages 348 - 352, XP002052220, ISSN: 0028-0836 *
MÜLLER U: "Ten years of gene targeting: Targeted mouse mutants, from vector design to phenotype analysis", MECHANISMS OF DEVELOPMENT, ELSEVIER SCIENCE IRELAND LTD, IE, vol. 82, no. 1-2, April 1999 (1999-04-01), pages 3 - 21, XP002208296, ISSN: 0925-4773 *

Also Published As

Publication number Publication date
WO2002055687A2 (fr) 2002-07-18

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