WO2000077250A3 - Procede de circularisation d'oligonucleotides autour d'un acide nucleique en double brin, les structures obtenues et leurs applications - Google Patents
Procede de circularisation d'oligonucleotides autour d'un acide nucleique en double brin, les structures obtenues et leurs applications Download PDFInfo
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- WO2000077250A3 WO2000077250A3 PCT/FR2000/001655 FR0001655W WO0077250A3 WO 2000077250 A3 WO2000077250 A3 WO 2000077250A3 FR 0001655 W FR0001655 W FR 0001655W WO 0077250 A3 WO0077250 A3 WO 0077250A3
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- Prior art keywords
- oligonucleotide
- nucleic acid
- single stranded
- stranded nucleic
- hybridized
- Prior art date
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- 108091034117 Oligonucleotide Proteins 0.000 title abstract 9
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000011159 matrix material Substances 0.000 abstract 3
- 230000000295 complement effect Effects 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 2
- 125000006850 spacer group Chemical group 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Le procédé de circularisation d'un oligonucléotide selon l'invention comprend: l'incubation d'un acide nucléique double brin comprenant une séquence cible, avec un oligonucléotide simple brin comprenant une partie centrale capable de se fixer sur ladite séquence cible, reliée par des séquences espaceurs à des séquences terminales en 5' et 3', ces séquences étant: soit a) complémentaires de la séquence d'une matrice oligonucléotidique; soit x) partiellement complémentaires l'une de l'autre, de manière à pouvoir s'hybrider l'une à l'autre en formant un double brin tout en conservant une extrémité en simple brin, laquelle est capable de s'hybrider à la séquence terminale d'un autre oligonucléotide, dit oligonucléotide-boucle, qui possède une structure en épingle à cheveux, avec une partie en simple brin et une partie repliée qui forme un double brin, la mise en contact, dans le premier cas a), du complexe formé avec ladite matrice oligonucléotidique, dans des conditions permettant l'hybridation des extrémités 5' et 3', de manière adjacente, à la matrice, et dans le cas x), celle du complexe formé avec ledit oligonucléotide-boucle, dans des conditions permettant, par l'hybridation des extrémités libres de l'oligonucléotide simple brin et de l'oligonucléotide-boucle, la juxtaposition des extrémités 5' et 3' de ces oligonucléotides, la ligation des extrémités hybridées, de manière à circulariser l'oligonucléotide autour de l'acide nucléique double brin et, si on le souhaite, l'isolement de la structure circularisée. Application du procédé et des structures obtenues en diagnostic et thérapeutique.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU62869/00A AU6286900A (en) | 1999-06-14 | 2000-06-14 | Method for circularizing oligonucleotides around a double stranded nucleic acid, resulting structures and uses thereof |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9907503A FR2794755A1 (fr) | 1999-06-14 | 1999-06-14 | Procede de circularisation d'oligonucleotides autour d'un acide nucleique en double brin, les structures obtenues et leurs applications |
| FR99/07503 | 1999-06-14 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2000077250A2 WO2000077250A2 (fr) | 2000-12-21 |
| WO2000077250A3 true WO2000077250A3 (fr) | 2001-08-30 |
Family
ID=9546756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/FR2000/001655 WO2000077250A2 (fr) | 1999-06-14 | 2000-06-14 | Procede de circularisation d'oligonucleotides autour d'un acide nucleique en double brin, les structures obtenues et leurs applications |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU6286900A (fr) |
| FR (1) | FR2794755A1 (fr) |
| WO (1) | WO2000077250A2 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE602006017192D1 (de) | 2005-04-12 | 2010-11-11 | Olink Ab | Verfahren zur herstellung von oligonukleotiden |
| WO2006108422A2 (fr) * | 2005-04-12 | 2006-10-19 | In Situ Rcp A/S Under Founding | Procedes de production d'oligonucleotides |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994017086A1 (fr) * | 1993-01-25 | 1994-08-04 | Apollon, Inc. | Regulation de genes par ciblage d'une helice triple intramoleculaire potentielle |
| US5591841A (en) * | 1993-01-14 | 1997-01-07 | Ji; Huamin | Rapid purification of circular DNA by triplex-mediated affinity capture |
| WO1997009069A1 (fr) * | 1995-09-08 | 1997-03-13 | Ulf Landegren | Procedes et compositions pour le ciblage d'acide nucleique |
| WO1997019193A2 (fr) * | 1995-11-21 | 1997-05-29 | Yale University | Amplication et detection de segments unimoleculaires |
| WO1997047769A1 (fr) * | 1996-06-14 | 1997-12-18 | Sarnoff Corporation | Detection par sonde cadenas |
| WO1998002449A1 (fr) * | 1996-07-16 | 1998-01-22 | Oncor, Inc. | Oligonucleotides marques par transfert d'energie moleculaire et amplifiant des acides nucleiques et procedes concernant lesdits oligonucleotides |
-
1999
- 1999-06-14 FR FR9907503A patent/FR2794755A1/fr not_active Withdrawn
-
2000
- 2000-06-14 WO PCT/FR2000/001655 patent/WO2000077250A2/fr active Application Filing
- 2000-06-14 AU AU62869/00A patent/AU6286900A/en not_active Abandoned
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5591841A (en) * | 1993-01-14 | 1997-01-07 | Ji; Huamin | Rapid purification of circular DNA by triplex-mediated affinity capture |
| WO1994017086A1 (fr) * | 1993-01-25 | 1994-08-04 | Apollon, Inc. | Regulation de genes par ciblage d'une helice triple intramoleculaire potentielle |
| WO1997009069A1 (fr) * | 1995-09-08 | 1997-03-13 | Ulf Landegren | Procedes et compositions pour le ciblage d'acide nucleique |
| WO1997019193A2 (fr) * | 1995-11-21 | 1997-05-29 | Yale University | Amplication et detection de segments unimoleculaires |
| WO1997047769A1 (fr) * | 1996-06-14 | 1997-12-18 | Sarnoff Corporation | Detection par sonde cadenas |
| WO1998002449A1 (fr) * | 1996-07-16 | 1998-01-22 | Oncor, Inc. | Oligonucleotides marques par transfert d'energie moleculaire et amplifiant des acides nucleiques et procedes concernant lesdits oligonucleotides |
Non-Patent Citations (4)
| Title |
|---|
| ESCUDÉ ET AL.: "Padlock oligonucleotides for duplex DNA based on sequence-specific triple helix formation", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 96, September 1999 (1999-09-01), WASHINGTON US, pages 10603 - 10607, XP002151269 * |
| ESCUDÉ ET AL.: "Rational design of a triple helix specific intercalating ligand", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 95, March 1998 (1998-03-01), WASHINGTON US, pages 3591 - 3596, XP002135278 * |
| NILSSON M ET AL: "PADLOCK PROBES: CIRCULARIZING OLIGONUCLEOTIDES FOR LOCALIZED DNA DETECTION", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 265, no. 5181, 30 September 1994 (1994-09-30), pages 2085 - 2088, XP000579803, ISSN: 0036-8075 * |
| PFANNSCHMIDT ET AL.: "Sequence-specific labeling of superhelical DNA by triple helix formation and psoralen crosslinking", NUCLEIC ACIDS RESEARCH, vol. 24, no. 9, 1996, OXFORD GB, pages 1702 - 1709, XP002135279 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000077250A2 (fr) | 2000-12-21 |
| FR2794755A1 (fr) | 2000-12-15 |
| AU6286900A (en) | 2001-01-02 |
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