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WO2000077250A3 - Method for circularizing oligonucleotides around a double stranded nucleic acid, resulting structures and uses thereof - Google Patents

Method for circularizing oligonucleotides around a double stranded nucleic acid, resulting structures and uses thereof Download PDF

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Publication number
WO2000077250A3
WO2000077250A3 PCT/FR2000/001655 FR0001655W WO0077250A3 WO 2000077250 A3 WO2000077250 A3 WO 2000077250A3 FR 0001655 W FR0001655 W FR 0001655W WO 0077250 A3 WO0077250 A3 WO 0077250A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotide
nucleic acid
single stranded
stranded nucleic
hybridized
Prior art date
Application number
PCT/FR2000/001655
Other languages
French (fr)
Other versions
WO2000077250A2 (en
Inventor
Christophe Escude
Therese Garestier
Claude Helene
Thibaut Roulon
Original Assignee
Inst Nat Sante Rech Med
Centre Nat Rech Scient
Christophe Escude
Therese Garestier
Claude Helene
Thibaut Roulon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Nat Sante Rech Med, Centre Nat Rech Scient, Christophe Escude, Therese Garestier, Claude Helene, Thibaut Roulon filed Critical Inst Nat Sante Rech Med
Priority to AU62869/00A priority Critical patent/AU6286900A/en
Publication of WO2000077250A2 publication Critical patent/WO2000077250A2/en
Publication of WO2000077250A3 publication Critical patent/WO2000077250A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention concerns a method for circularizing an oligonucleotide which consists in: incubating a double stranded nucleic acid comprising a target sequence, with a single stranded oligonucleotide comprising a central part capable of being fixed on said target sequence, bound by spacer segments to 5' and 3' terminal sequences, said sequences being: either a) complementary to the sequence of an oligonucleotide matrix; or x) partly complementary to each other, so as to be capable of being hybridized to each other forming a double strand while preserving a single stranded end, which is capable of being hybridized with the terminal sequence of another oligonucleotide, called loop-oligonucleotide, which has a hairpin structure, with one single stranded part and a folded part forming a double strand; contacting, in the first case a) the complex formed with said oligonucleotide matrix, in conditions enabling the hybridization of the 3' and 5' ends, adjacently, with the matrix, and in the case x), contacting the complex formed with said loop-oligonucleotide, in conditions enabling, through hybridization of the free ends of the single stranded oligonucleotides and of the loop-oligonucleotide, the juxtaposition of the 3' and 5' ends of said oligonucleotides; ligating the hybridized ends so as to circularize the oligonucleotide around the double stranded nucleic acid and, optionally, isolating the circularized structure. The invention also concerns the applications and the resulting structures obtained for diagnostic and therapeutic purposes.
PCT/FR2000/001655 1999-06-14 2000-06-14 Method for circularizing oligonucleotides around a double stranded nucleic acid, resulting structures and uses thereof WO2000077250A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU62869/00A AU6286900A (en) 1999-06-14 2000-06-14 Method for circularizing oligonucleotides around a double stranded nucleic acid, resulting structures and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9907503A FR2794755A1 (en) 1999-06-14 1999-06-14 PROCESS FOR CIRCULARIZING OLIGONUCLEOTIDES AROUND A DOUBLE-STRANDED NUCLEIC ACID, THE STRUCTURES OBTAINED AND THEIR APPLICATIONS
FR99/07503 1999-06-14

Publications (2)

Publication Number Publication Date
WO2000077250A2 WO2000077250A2 (en) 2000-12-21
WO2000077250A3 true WO2000077250A3 (en) 2001-08-30

Family

ID=9546756

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2000/001655 WO2000077250A2 (en) 1999-06-14 2000-06-14 Method for circularizing oligonucleotides around a double stranded nucleic acid, resulting structures and uses thereof

Country Status (3)

Country Link
AU (1) AU6286900A (en)
FR (1) FR2794755A1 (en)
WO (1) WO2000077250A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602006017192D1 (en) 2005-04-12 2010-11-11 Olink Ab PROCESS FOR THE PREPARATION OF OLIGONUCLEOTIDES
WO2006108422A2 (en) * 2005-04-12 2006-10-19 In Situ Rcp A/S Under Founding Methods for production of oligonucleotides

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994017086A1 (en) * 1993-01-25 1994-08-04 Apollon, Inc. Gene regulation by targeting putative intramolecular triple helix
US5591841A (en) * 1993-01-14 1997-01-07 Ji; Huamin Rapid purification of circular DNA by triplex-mediated affinity capture
WO1997009069A1 (en) * 1995-09-08 1997-03-13 Ulf Landegren Methods and compositions for nucleic acid targeting
WO1997019193A2 (en) * 1995-11-21 1997-05-29 Yale University Unimolecular segment amplification and detection
WO1997047769A1 (en) * 1996-06-14 1997-12-18 Sarnoff Corporation Padlock probe detection
WO1998002449A1 (en) * 1996-07-16 1998-01-22 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591841A (en) * 1993-01-14 1997-01-07 Ji; Huamin Rapid purification of circular DNA by triplex-mediated affinity capture
WO1994017086A1 (en) * 1993-01-25 1994-08-04 Apollon, Inc. Gene regulation by targeting putative intramolecular triple helix
WO1997009069A1 (en) * 1995-09-08 1997-03-13 Ulf Landegren Methods and compositions for nucleic acid targeting
WO1997019193A2 (en) * 1995-11-21 1997-05-29 Yale University Unimolecular segment amplification and detection
WO1997047769A1 (en) * 1996-06-14 1997-12-18 Sarnoff Corporation Padlock probe detection
WO1998002449A1 (en) * 1996-07-16 1998-01-22 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ESCUDÉ ET AL.: "Padlock oligonucleotides for duplex DNA based on sequence-specific triple helix formation", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 96, September 1999 (1999-09-01), WASHINGTON US, pages 10603 - 10607, XP002151269 *
ESCUDÉ ET AL.: "Rational design of a triple helix specific intercalating ligand", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 95, March 1998 (1998-03-01), WASHINGTON US, pages 3591 - 3596, XP002135278 *
NILSSON M ET AL: "PADLOCK PROBES: CIRCULARIZING OLIGONUCLEOTIDES FOR LOCALIZED DNA DETECTION", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 265, no. 5181, 30 September 1994 (1994-09-30), pages 2085 - 2088, XP000579803, ISSN: 0036-8075 *
PFANNSCHMIDT ET AL.: "Sequence-specific labeling of superhelical DNA by triple helix formation and psoralen crosslinking", NUCLEIC ACIDS RESEARCH, vol. 24, no. 9, 1996, OXFORD GB, pages 1702 - 1709, XP002135279 *

Also Published As

Publication number Publication date
WO2000077250A2 (en) 2000-12-21
FR2794755A1 (en) 2000-12-15
AU6286900A (en) 2001-01-02

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