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WO1999034002A3 - Proteases provenant d'organismes a gram positif - Google Patents

Proteases provenant d'organismes a gram positif Download PDF

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Publication number
WO1999034002A3
WO1999034002A3 PCT/US1998/027018 US9827018W WO9934002A3 WO 1999034002 A3 WO1999034002 A3 WO 1999034002A3 US 9827018 W US9827018 W US 9827018W WO 9934002 A3 WO9934002 A3 WO 9934002A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
provides
proteases
gram positive
Prior art date
Application number
PCT/US1998/027018
Other languages
English (en)
Other versions
WO1999034002A2 (fr
Inventor
David A Estell
Original Assignee
Genencor Int
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, David A Estell filed Critical Genencor Int
Priority to US09/555,062 priority Critical patent/US6528255B1/en
Priority to AU19299/99A priority patent/AU1929999A/en
Priority to EP98964106A priority patent/EP1042489A2/fr
Publication of WO1999034002A2 publication Critical patent/WO1999034002A2/fr
Publication of WO1999034002A3 publication Critical patent/WO1999034002A3/fr
Priority to US10/285,074 priority patent/US7078216B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

L'invention concerne l'identification d'une nouvelle métalloprotéase dans des micro-organismes à Gram positif. Elle concerne également les séquences d'acides nucléiques et d'acides aminés de cette métalloprotéase; des cellules hôtes présentant une mutation ou une délétion d'une partie ou de la totalité du gène codant la métalloprotéase; des cellules hôtes contenant, de plus, un acide nucléique codant des protéines hétérologues souhaitées, telles que des enzymes; des compositions de nettoyage, des aliments pour animaux et des compositions utilisées pour traiter un textile, qui contiennent cette métalloprotéase.
PCT/US1998/027018 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif WO1999034002A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US09/555,062 US6528255B1 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
AU19299/99A AU1929999A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
EP98964106A EP1042489A2 (fr) 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif
US10/285,074 US7078216B2 (en) 1997-12-30 2002-10-31 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727466.6A GB9727466D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727466.6 1997-12-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09555062 A-371-Of-International 1998-12-17
US10/285,074 Division US7078216B2 (en) 1997-12-30 2002-10-31 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999034002A2 WO1999034002A2 (fr) 1999-07-08
WO1999034002A3 true WO1999034002A3 (fr) 1999-11-04

Family

ID=10824310

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027018 WO1999034002A2 (fr) 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif

Country Status (4)

Country Link
EP (1) EP1042489A2 (fr)
AU (1) AU1929999A (fr)
GB (1) GB9727466D0 (fr)
WO (1) WO1999034002A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, 1 January 1900, Columbus, Ohio, US; abstract no. Y14083, XP002104304 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002104305, Database accession no. Z99109 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002104306, Database accession no. H69817 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, no. 4, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
GB9727466D0 (en) 1998-02-25
AU1929999A (en) 1999-07-19
WO1999034002A2 (fr) 1999-07-08
EP1042489A2 (fr) 2000-10-11

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