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WO1999034002A3 - Proteases from gram positive organisms - Google Patents

Proteases from gram positive organisms Download PDF

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Publication number
WO1999034002A3
WO1999034002A3 PCT/US1998/027018 US9827018W WO9934002A3 WO 1999034002 A3 WO1999034002 A3 WO 1999034002A3 US 9827018 W US9827018 W US 9827018W WO 9934002 A3 WO9934002 A3 WO 9934002A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
provides
proteases
gram positive
Prior art date
Application number
PCT/US1998/027018
Other languages
French (fr)
Other versions
WO1999034002A2 (en
Inventor
David A Estell
Original Assignee
Genencor Int
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, David A Estell filed Critical Genencor Int
Priority to EP98964106A priority Critical patent/EP1042489A2/en
Priority to US09/555,062 priority patent/US6528255B1/en
Priority to AU19299/99A priority patent/AU1929999A/en
Publication of WO1999034002A2 publication Critical patent/WO1999034002A2/en
Publication of WO1999034002A3 publication Critical patent/WO1999034002A3/en
Priority to US10/285,074 priority patent/US7078216B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

The present invention relates to the identification of a novel metalloprotease in Bacillus subtilis. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
PCT/US1998/027018 1997-12-30 1998-12-17 Proteases from gram positive organisms WO1999034002A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP98964106A EP1042489A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
US09/555,062 US6528255B1 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
AU19299/99A AU1929999A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
US10/285,074 US7078216B2 (en) 1997-12-30 2002-10-31 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9727466.6 1997-12-30
GBGB9727466.6A GB9727466D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09555062 A-371-Of-International 1998-12-17
US10/285,074 Division US7078216B2 (en) 1997-12-30 2002-10-31 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999034002A2 WO1999034002A2 (en) 1999-07-08
WO1999034002A3 true WO1999034002A3 (en) 1999-11-04

Family

ID=10824310

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027018 WO1999034002A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms

Country Status (4)

Country Link
EP (1) EP1042489A2 (en)
AU (1) AU1929999A (en)
GB (1) GB9727466D0 (en)
WO (1) WO1999034002A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, 1 January 1900, Columbus, Ohio, US; abstract no. Y14083, XP002104304 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002104305, Database accession no. Z99109 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002104306, Database accession no. H69817 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, no. 4, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
EP1042489A2 (en) 2000-10-11
WO1999034002A2 (en) 1999-07-08
GB9727466D0 (en) 1998-02-25
AU1929999A (en) 1999-07-19

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