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WO1999034003A3 - Proteases provenant d'organismes a gram positif - Google Patents

Proteases provenant d'organismes a gram positif Download PDF

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Publication number
WO1999034003A3
WO1999034003A3 PCT/US1998/027020 US9827020W WO9934003A3 WO 1999034003 A3 WO1999034003 A3 WO 1999034003A3 US 9827020 W US9827020 W US 9827020W WO 9934003 A3 WO9934003 A3 WO 9934003A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
gram positive
provides
proteases
Prior art date
Application number
PCT/US1998/027020
Other languages
English (en)
Other versions
WO1999034003A2 (fr
Inventor
David A Estell
Original Assignee
Genencor Int
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, David A Estell filed Critical Genencor Int
Priority to EP98964108A priority Critical patent/EP1042490A2/fr
Priority to AU19301/99A priority patent/AU1930199A/en
Priority to US09/554,998 priority patent/US6599731B1/en
Publication of WO1999034003A2 publication Critical patent/WO1999034003A2/fr
Publication of WO1999034003A3 publication Critical patent/WO1999034003A3/fr
Priority to US11/013,992 priority patent/US20050101504A1/en
Priority to US11/014,361 priority patent/US7098021B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne l'identification d'une nouvelle métalloprotéase dans des micro-organismes à Gram positif. Elle concerne également les séquences d'acides nucléiques et d'acides aminés de cette métalloprotéase; des cellules hôtes présentant une mutation ou une délétion d'une partie ou de la totalité du gène codant la métalloprotéase; des cellules hôtes contenant, de plus, un acide nucléique codant des protéines hétérologues souhaitées, telles que des enzymes; des compositions de nettoyage, des aliments pour animaux et des compositions utilisées pour traiter un textile, qui contiennent cette métalloprotéase.
PCT/US1998/027020 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif WO1999034003A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP98964108A EP1042490A2 (fr) 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif
AU19301/99A AU1930199A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
US09/554,998 US6599731B1 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
US11/013,992 US20050101504A1 (en) 1997-12-30 2004-12-16 Proteases from gram positive organisms
US11/014,361 US7098021B2 (en) 1997-12-30 2004-12-16 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727464.1A GB9727464D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727464.1 1997-12-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09554998 A-371-Of-International 1998-12-17
US10/460,718 Division US6872807B2 (en) 1997-12-30 2003-06-12 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999034003A2 WO1999034003A2 (fr) 1999-07-08
WO1999034003A3 true WO1999034003A3 (fr) 1999-11-18

Family

ID=10824308

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027020 WO1999034003A2 (fr) 1997-12-30 1998-12-17 Proteases provenant d'organismes a gram positif

Country Status (4)

Country Link
EP (1) EP1042490A2 (fr)
AU (1) AU1930199A (fr)
GB (1) GB9727464D0 (fr)
WO (1) WO1999034003A2 (fr)

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GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
BR0214653B1 (pt) 2001-12-07 2015-01-06 Novozymes As Construção de ácido nucléico, vetor de expressão recombinante, célula de microorganismo hospedeiro recombinante, método para produzir um polipeptídeo, uso de pelo menos uma protease, método para melhorar o valor nutricional de uma ração animal, aditivo para ração animal, composição de ração animal, e, método para o tratamento de proteínas egetais”.
CN102414317B (zh) * 2009-02-27 2014-11-05 诺维信公司 具有减少的金属肽酶表达、适于重组多肽产生的突变细胞
DK3514230T3 (da) 2013-12-13 2021-11-15 Danisco Us Inc Serinproteaser af bacillus-arter
WO2015089447A1 (fr) 2013-12-13 2015-06-18 Danisco Us Inc. Sérines protéases du clade du bacillus gibsonii
AU2014366222B2 (en) 2013-12-16 2018-07-19 Nutrition & Biosciences USA 4, Inc. Use of poly alpha-1,3-glucan ethers as viscosity modifiers
EP3789407B1 (fr) 2013-12-18 2024-07-24 Nutrition & Biosciences USA 4, Inc. Éthers cationiques de poly(alpha-1,3-glucane)
EP3105256A1 (fr) 2014-02-14 2016-12-21 E. I. du Pont de Nemours and Company Poly-alpha-1,3-1,6-glucanes utilisables en vue de la modification de la viscosité
US9695253B2 (en) 2014-03-11 2017-07-04 E I Du Pont De Nemours And Company Oxidized poly alpha-1,3-glucan
US20170096653A1 (en) 2014-03-21 2017-04-06 Danisco Us Inc. Serine proteases of bacillus species
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
US9771548B2 (en) 2014-06-19 2017-09-26 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3207129B1 (fr) 2014-10-17 2019-11-20 Danisco US Inc. Sérines protéases de l'espèce bacillus
CN107148472A (zh) 2014-10-27 2017-09-08 丹尼斯科美国公司 芽孢杆菌属物种的丝氨酸蛋白酶
EP3212781B1 (fr) 2014-10-27 2019-09-18 Danisco US Inc. Sérine-protéases
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
EP3550017B1 (fr) 2014-10-27 2021-07-14 Danisco US Inc. Sérine protéases
WO2016069544A1 (fr) 2014-10-27 2016-05-06 Danisco Us Inc. Sérines protéases
JP2018512109A (ja) 2014-12-23 2018-05-17 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 酵素により製造されるセルロース
EP3294884B1 (fr) 2015-05-13 2021-01-27 Danisco US Inc. Variants de protéase aprl-clade et leurs utilisations
CN107849549B (zh) 2015-06-17 2024-04-05 丹尼斯科美国公司 吉氏芽孢杆菌进化枝丝氨酸蛋白酶
EP4483721A3 (fr) 2015-11-05 2025-04-16 Danisco US Inc. Paenibacillus et bacillus spp. mannanases
JP7364331B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス（Ｐａｅｎｉｂａｃｉｌｌｕｓ）属種マンナナーゼ
WO2017083229A1 (fr) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Compositions de fibres de glucane utiles pour la lessive et l'entretien des tissus
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
EP3374400B1 (fr) 2015-11-13 2022-04-13 Nutrition & Biosciences USA 4, Inc. Compositions de fibres de glucane utiles pour la lessive et l'entretien des tissus
US20180362946A1 (en) 2015-12-18 2018-12-20 Danisco Us Inc. Polypeptides with endoglucanase activity and uses thereof
CN119307476A (zh) 2016-05-31 2025-01-14 丹尼斯科美国公司 蛋白酶变体及其用途
EP4527923A2 (fr) 2016-06-17 2025-03-26 Danisco US Inc Variantes de protéase et leurs utilisations
WO2018085524A2 (fr) 2016-11-07 2018-05-11 Danisco Us Inc Composition détergente pour le linge
US20200392477A1 (en) 2016-12-21 2020-12-17 Danisco Us Inc. Protease variants and uses thereof
WO2018118950A1 (fr) 2016-12-21 2018-06-28 Danisco Us Inc. Sérine-protéases du clade du bacillus gibsonii
CN110621778A (zh) 2017-03-15 2019-12-27 丹尼斯科美国公司 胰蛋白酶样丝氨酸蛋白酶及其用途
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
US20210214703A1 (en) 2018-06-19 2021-07-15 Danisco Us Inc Subtilisin variants
WO2019245705A1 (fr) 2018-06-19 2019-12-26 Danisco Us Inc Variants de subtilisine
CN113166682A (zh) 2018-09-27 2021-07-23 丹尼斯科美国公司 用于医疗器械清洁的组合物
US20230028935A1 (en) 2018-11-28 2023-01-26 Danisco Us Inc Subtilisin variants having improved stability
WO2020242858A1 (fr) 2019-05-24 2020-12-03 Danisco Us Inc Variants de subtilisine et procédés d'utilisation
EP3980517A1 (fr) 2019-06-06 2022-04-13 Danisco US Inc. Procédés et compositions de nettoyage
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
WO2022165107A1 (fr) 2021-01-29 2022-08-04 Danisco Us Inc Compositions pour le nettoyage et procédés associés
US20240294888A1 (en) 2021-06-30 2024-09-05 Danisco Us Inc. Variant enzymes and uses thereof
EP4396320A2 (fr) 2021-09-03 2024-07-10 Danisco US Inc. Compositions de blanchisserie pour le nettoyage
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CN119855892A (zh) 2022-09-02 2025-04-18 丹尼斯科美国公司 洗涤剂组合物及其相关方法
WO2024050343A1 (fr) 2022-09-02 2024-03-07 Danisco Us Inc. Variants de subtilisine et procédés associés
WO2024102698A1 (fr) 2022-11-09 2024-05-16 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2024163584A1 (fr) 2023-02-01 2024-08-08 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2024186819A1 (fr) 2023-03-06 2024-09-12 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2024191711A1 (fr) 2023-03-16 2024-09-19 Nutrition & Biosciences USA 4, Inc. Extraits fermentés de brevibacillus pour le nettoyage et la lutte contre les mauvaises odeurs et leur utilisation
WO2025071996A1 (fr) 2023-09-28 2025-04-03 Danisco Us Inc. Variant d'enzymes cutinases à solubilité améliorée et leurs utilisations
WO2025085351A1 (fr) 2023-10-20 2025-04-24 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus
FR2728905A1 (fr) * 1994-12-29 1996-07-05 Rhone Poulenc Nutrition Animal Nouvelle acide amine amidohydrolase, sequence nuclotidique correspondant et leurs utilisations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus
FR2728905A1 (fr) * 1994-12-29 1996-07-05 Rhone Poulenc Nutrition Animal Nouvelle acide amine amidohydrolase, sequence nuclotidique correspondant et leurs utilisations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002106131, Database accession no. Z99120 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002106132, Database accession no. G70017 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
GB9727464D0 (en) 1998-02-25
AU1930199A (en) 1999-07-19
WO1999034003A2 (fr) 1999-07-08
EP1042490A2 (fr) 2000-10-11

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