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WO1999033960A3 - Proteases from gram positive organisms - Google Patents

Proteases from gram positive organisms Download PDF

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Publication number
WO1999033960A3
WO1999033960A3 PCT/US1998/027040 US9827040W WO9933960A3 WO 1999033960 A3 WO1999033960 A3 WO 1999033960A3 US 9827040 W US9827040 W US 9827040W WO 9933960 A3 WO9933960 A3 WO 9933960A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
gram positive
provides
proteases
Prior art date
Application number
PCT/US1998/027040
Other languages
French (fr)
Other versions
WO1999033960A2 (en
Inventor
David A Estell
Original Assignee
Genencor Int
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US09/554,999 priority Critical patent/US6465186B1/en
Application filed by Genencor Int, David A Estell filed Critical Genencor Int
Priority to AU18327/99A priority patent/AU1832799A/en
Priority to EP98963276A priority patent/EP1042487A2/en
Publication of WO1999033960A2 publication Critical patent/WO1999033960A2/en
Publication of WO1999033960A3 publication Critical patent/WO1999033960A3/en
Priority to US10/188,586 priority patent/US6794179B2/en
Priority to US10/925,652 priority patent/US7070819B2/en
Priority to US10/926,197 priority patent/US7070986B2/en
Priority to US10/925,708 priority patent/US7070990B2/en
Priority to US10/925,821 priority patent/US7078372B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Animal Husbandry (AREA)
  • Textile Engineering (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
PCT/US1998/027040 1997-12-30 1998-12-17 Proteases from gram positive organisms WO1999033960A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
US09/554,999 US6465186B1 (en) 1997-12-30 1998-12-01 Proteases from gram positive organisms
AU18327/99A AU1832799A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
EP98963276A EP1042487A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
US10/188,586 US6794179B2 (en) 1997-12-30 2002-07-02 Proteases from gram positive organisms
US10/925,652 US7070819B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms
US10/926,197 US7070986B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms
US10/925,708 US7070990B2 (en) 1997-12-30 2004-08-24 Proteases from GRAM positive organisms
US10/925,821 US7078372B2 (en) 1997-12-30 2004-08-24 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727471.6A GB9727471D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727471.6 1997-12-30

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09/554,999 Continuation US6465186B1 (en) 1997-12-30 1998-12-01 Proteases from gram positive organisms
US09554999 A-371-Of-International 1998-12-17
US10/188,586 Division US6794179B2 (en) 1997-12-30 2002-07-02 Proteases from gram positive organisms

Publications (2)

Publication Number Publication Date
WO1999033960A2 WO1999033960A2 (en) 1999-07-08
WO1999033960A3 true WO1999033960A3 (en) 2000-03-02

Family

ID=10824314

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/027040 WO1999033960A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms

Country Status (4)

Country Link
EP (1) EP1042487A2 (en)
AU (1) AU1832799A (en)
GB (1) GB9727471D0 (en)
WO (1) WO1999033960A2 (en)

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IL136479A0 (en) * 1997-12-09 2001-06-14 Univ California Block polymer processing for mesostructured inorganic oxide materials
GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
US20060121565A1 (en) * 2002-09-20 2006-06-08 Cymip A/S Methods for increasing the production of a recombinant polypeptide from a host cell
DK3514230T3 (en) 2013-12-13 2021-11-15 Danisco Us Inc SERIN PROTEASES OF BACILLUS SPECIES
WO2015089447A1 (en) 2013-12-13 2015-06-18 Danisco Us Inc. Serine proteases of the bacillus gibsonii-clade
AU2014366222B2 (en) 2013-12-16 2018-07-19 Nutrition & Biosciences USA 4, Inc. Use of poly alpha-1,3-glucan ethers as viscosity modifiers
EP3789407B1 (en) 2013-12-18 2024-07-24 Nutrition & Biosciences USA 4, Inc. Cationic poly alpha-1,3-glucan ethers
EP3105256A1 (en) 2014-02-14 2016-12-21 E. I. du Pont de Nemours and Company Poly-alpha-1,3-1,6-glucans for viscosity modification
US9695253B2 (en) 2014-03-11 2017-07-04 E I Du Pont De Nemours And Company Oxidized poly alpha-1,3-glucan
US20170096653A1 (en) 2014-03-21 2017-04-06 Danisco Us Inc. Serine proteases of bacillus species
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
US9771548B2 (en) 2014-06-19 2017-09-26 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
EP3207129B1 (en) 2014-10-17 2019-11-20 Danisco US Inc. Serine proteases of bacillus species
WO2016069544A1 (en) 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
EP3212781B1 (en) 2014-10-27 2019-09-18 Danisco US Inc. Serine proteases
CN107148472A (en) 2014-10-27 2017-09-08 丹尼斯科美国公司 Serine proteases from Bacillus sp.
DK3212662T3 (en) 2014-10-27 2020-07-20 Danisco Us Inc serine proteases
EP3550017B1 (en) 2014-10-27 2021-07-14 Danisco US Inc. Serine proteases
JP2018512109A (en) 2014-12-23 2018-05-17 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ Cellulose produced by enzymes
EP3294884B1 (en) 2015-05-13 2021-01-27 Danisco US Inc. Aprl-clade protease variants and uses thereof
CN107849549B (en) 2015-06-17 2024-04-05 丹尼斯科美国公司 Geobacillus (Ji's bacillus) bacillus evolution branch serine protease enzyme
EP4483721A3 (en) 2015-11-05 2025-04-16 Danisco US Inc. Paenibacillus and bacillus spp. mannanases
JP7364331B2 (en) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク Paenibacillus sp. mannanase
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
WO2017083229A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
EP3374400B1 (en) 2015-11-13 2022-04-13 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
US20180362946A1 (en) 2015-12-18 2018-12-20 Danisco Us Inc. Polypeptides with endoglucanase activity and uses thereof
CN119307476A (en) 2016-05-31 2025-01-14 丹尼斯科美国公司 Protease variants and uses thereof
EP4527923A2 (en) 2016-06-17 2025-03-26 Danisco US Inc Protease variants and uses thereof
WO2018085524A2 (en) 2016-11-07 2018-05-11 Danisco Us Inc Laundry detergent composition
US20200392477A1 (en) 2016-12-21 2020-12-17 Danisco Us Inc. Protease variants and uses thereof
WO2018118950A1 (en) 2016-12-21 2018-06-28 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
CN110621778A (en) 2017-03-15 2019-12-27 丹尼斯科美国公司 Trypsin-like serine protease and uses thereof
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
WO2019245705A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
US20210214703A1 (en) 2018-06-19 2021-07-15 Danisco Us Inc Subtilisin variants
CN113166682A (en) 2018-09-27 2021-07-23 丹尼斯科美国公司 Composition for medical device cleaning
US20230028935A1 (en) 2018-11-28 2023-01-26 Danisco Us Inc Subtilisin variants having improved stability
WO2020242858A1 (en) 2019-05-24 2020-12-03 Danisco Us Inc Subtilisin variants and methods of use
EP3980517A1 (en) 2019-06-06 2022-04-13 Danisco US Inc. Methods and compositions for cleaning
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
WO2022165107A1 (en) 2021-01-29 2022-08-04 Danisco Us Inc Compositions for cleaning and methods related thereto
US20240294888A1 (en) 2021-06-30 2024-09-05 Danisco Us Inc. Variant enzymes and uses thereof
EP4396320A2 (en) 2021-09-03 2024-07-10 Danisco US Inc. Laundry compositions for cleaning
WO2023114936A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
CN118679252A (en) 2021-12-16 2024-09-20 丹尼斯科美国公司 Subtilisin variants and methods of use
CN118974227A (en) 2022-03-01 2024-11-15 丹尼斯科美国公司 Enzymes and enzyme compositions for cleaning
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
CN119855892A (en) 2022-09-02 2025-04-18 丹尼斯科美国公司 Detergent compositions and methods relating thereto
WO2024050343A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Subtilisin variants and methods related thereto
CN119816592A (en) 2022-09-02 2025-04-11 丹尼斯科美国公司 Mannanase variants and methods of use
WO2024102698A1 (en) 2022-11-09 2024-05-16 Danisco Us Inc. Subtilisin variants and methods of use
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
WO2024186819A1 (en) 2023-03-06 2024-09-12 Danisco Us Inc. Subtilisin variants and methods of use
WO2024191711A1 (en) 2023-03-16 2024-09-19 Nutrition & Biosciences USA 4, Inc. Brevibacillus fermentate extracts for cleaning and malodor control and use thereof
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use

Citations (1)

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Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002115981, Database accession no. Z99112 *
DATABASE MPSRCH GENBANK 1 January 1900 (1900-01-01), XP002115982, Database accession no. G69885 *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
EP1042487A2 (en) 2000-10-11
AU1832799A (en) 1999-07-19
WO1999033960A2 (en) 1999-07-08
GB9727471D0 (en) 1998-02-25

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