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WO1998046996A3 - Lck serine phosphorylation assay for t-cell activation and activation-induced cell death - Google Patents

Lck serine phosphorylation assay for t-cell activation and activation-induced cell death Download PDF

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Publication number
WO1998046996A3
WO1998046996A3 PCT/IB1998/000801 IB9800801W WO9846996A3 WO 1998046996 A3 WO1998046996 A3 WO 1998046996A3 IB 9800801 W IB9800801 W IB 9800801W WO 9846996 A3 WO9846996 A3 WO 9846996A3
Authority
WO
WIPO (PCT)
Prior art keywords
activation
lck
serine phosphorylation
phosphorylation assay
cell
Prior art date
Application number
PCT/IB1998/000801
Other languages
French (fr)
Other versions
WO1998046996A2 (en
WO1998046996A9 (en
Inventor
Joaquim Madrenas
Original Assignee
Robarts John P Res Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robarts John P Res Inst filed Critical Robarts John P Res Inst
Priority to AU72283/98A priority Critical patent/AU7228398A/en
Publication of WO1998046996A2 publication Critical patent/WO1998046996A2/en
Publication of WO1998046996A3 publication Critical patent/WO1998046996A3/en
Publication of WO1998046996A9 publication Critical patent/WO1998046996A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention features an assay which allows the correlation between the ratio of serine phosphorylated lck to total lck with the level of T cell activation. This assay may be used for the rapid determination of the efficacies of therapies which either induce or prevent T cell activation. Also provided are src-family tyrosine kinase polypeptides which have reduced serine phosphorylation-mediated degradation.
PCT/IB1998/000801 1997-04-11 1998-04-10 Lck serine phosphorylation assay for t-cell activation and activation-induced cell death WO1998046996A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU72283/98A AU7228398A (en) 1997-04-11 1998-04-10 Lck serine phosphorylation assay for t-cell activation and activation-induced cell death

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4187897P 1997-04-11 1997-04-11
US60/041,878 1997-04-11

Publications (3)

Publication Number Publication Date
WO1998046996A2 WO1998046996A2 (en) 1998-10-22
WO1998046996A3 true WO1998046996A3 (en) 1999-04-01
WO1998046996A9 WO1998046996A9 (en) 1999-05-06

Family

ID=21918830

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1998/000801 WO1998046996A2 (en) 1997-04-11 1998-04-10 Lck serine phosphorylation assay for t-cell activation and activation-induced cell death

Country Status (2)

Country Link
AU (1) AU7228398A (en)
WO (1) WO1998046996A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7109003B2 (en) 1998-12-23 2006-09-19 Abgenix, Inc. Methods for expressing and recovering human monoclonal antibodies to CTLA-4
EE05627B1 (en) 1998-12-23 2013-02-15 Pfizer Inc. Human monoclonal antibodies to CTLA-4
ATE464383T1 (en) 1999-08-05 2010-04-15 Greenpeptide Co Ltd TUMOR ANTIGEN
NZ517202A (en) 1999-08-24 2004-05-28 Medarex Inc Human CTLA-4 antibodies and their uses
US7605238B2 (en) 1999-08-24 2009-10-20 Medarex, Inc. Human CTLA-4 antibodies and their uses
IL164287A0 (en) 2002-04-12 2005-12-18 Medarex Inc Methods of treatment using ctla-4 antibodies
CA2630157C (en) 2005-12-07 2018-01-09 Medarex, Inc. Ctla-4 antibody dosage escalation regimens

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GOLD, M.R.: "Activation and serine phosphorylation of the p56lck protein tyrosine kinase in response to antigen receptor cross-linking in B lymphocytes", THE JOURNAL OF IMMUNOLOGY, vol. 153, no. 6, 15 September 1994 (1994-09-15), pages 2369 - 2380, XP002079588 *
HORAK, I.D: ET AL.: "T.lymphocyte interleukin-2 dependent tyrosine protein kinase signal transduction involves the activation of p56lck", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 88, March 1991 (1991-03-01), WASHINGTON US, pages 1996 - 2000, XP002079586 *
LEE, J.E. ET AL.: "Inactivation of lck and loss of TCR-mediated signalling upon persistent engagment with complexes of peptide:MHC molecules.", THE JOURNAL OF IMMUNOLOGY, vol. 159, no. 1, 1 July 1997 (1997-07-01), pages 61 - 69, XP002079590 *
MARTH, J.D. ET AL.: "Lymphocyte activation provokes modiication of a lymphocyte-specific proetin tyrosine kinase (p56lck)", JOURNAL OF IMMUNOLOGY, vol. 142, no. 7, 1 April 1989 (1989-04-01), BALTIMORE US, pages 2430 - 2437, XP002079587 *
PATHAN, N. I. ET AL.,: "The protein kinase lck associates with and is phosphorylated by cdc2", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 44, 1 November 1996 (1996-11-01), pages 27517 - 27523, XP002079585 *
REPKE, H. ET AL.: "ganglioside-induced CD4 endocytosis occurs independent of serine phosphorylation and is accompanied by dissociation of p56lck", THE JOURNAL OF IMMUNOLOGY, vol. 149, no. 8, 15 October 1992 (1992-10-15), pages 2585 - 2591, XP002079589 *

Also Published As

Publication number Publication date
WO1998046996A2 (en) 1998-10-22
AU7228398A (en) 1998-11-11
WO1998046996A9 (en) 1999-05-06

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