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WO2001067106A3 - Identification of g protein-coupled receptor ligands and modulators - Google Patents

Identification of g protein-coupled receptor ligands and modulators Download PDF

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Publication number
WO2001067106A3
WO2001067106A3 PCT/US2001/007304 US0107304W WO0167106A3 WO 2001067106 A3 WO2001067106 A3 WO 2001067106A3 US 0107304 W US0107304 W US 0107304W WO 0167106 A3 WO0167106 A3 WO 0167106A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
modulators
identification
mutants
coupled receptor
Prior art date
Application number
PCT/US2001/007304
Other languages
French (fr)
Other versions
WO2001067106A2 (en
WO2001067106A8 (en
Inventor
Gabriel Berstein
Original Assignee
Millennium Pharm Inc
Gabriel Berstein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Millennium Pharm Inc, Gabriel Berstein filed Critical Millennium Pharm Inc
Priority to AU2001249107A priority Critical patent/AU2001249107A1/en
Publication of WO2001067106A2 publication Critical patent/WO2001067106A2/en
Publication of WO2001067106A8 publication Critical patent/WO2001067106A8/en
Publication of WO2001067106A3 publication Critical patent/WO2001067106A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention features methods of assaying for ligands and/or modulators of G protein-coupled receptors, for example, orphan G protein-coupled receptors. The methods of the invention feature constitutively active arrestin mutants, in particular, phosphorylation independent mutants. Also described are specific phosphorylation independent arrestin mutants and methods of making such mutants.
PCT/US2001/007304 2000-03-03 2001-03-05 Identification of g protein-coupled receptor ligands and modulators WO2001067106A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001249107A AU2001249107A1 (en) 2000-03-03 2001-03-05 Methods of assaying for g protein-coupled receptor ligands and modulators

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18670600P 2000-03-03 2000-03-03
US60/186,706 2000-03-03

Publications (3)

Publication Number Publication Date
WO2001067106A2 WO2001067106A2 (en) 2001-09-13
WO2001067106A8 WO2001067106A8 (en) 2002-02-14
WO2001067106A3 true WO2001067106A3 (en) 2002-04-11

Family

ID=22685983

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/007304 WO2001067106A2 (en) 2000-03-03 2001-03-05 Identification of g protein-coupled receptor ligands and modulators

Country Status (2)

Country Link
AU (1) AU2001249107A1 (en)
WO (1) WO2001067106A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002046763A1 (en) * 2000-12-04 2002-06-13 Novo Nordisk A/S Method of identifying compounds capable of acting as agonists or antagonists of g-protein coupled receptors
AU2002358469A1 (en) * 2001-12-21 2003-07-15 7Tm Pharma A/S Modified receptors for the discovery of therapeutic ligands
US20060099646A1 (en) * 2002-10-11 2006-05-11 Anders Heding Bret assay
EP1599492A4 (en) * 2003-01-17 2008-09-24 Virologic Inc Receptor deorphanization using tagged molecular libraries
US20060246507A1 (en) * 2003-01-22 2006-11-02 7Tm Pharma A/S Beta-arrestin based screening assays
FR2934684B1 (en) 2008-07-31 2012-11-16 Cis Bio Int METHOD OF DETECTING INTERNALIZATION OF MEMBRANE PROTEINS.

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BARAK L S ET AL: "A beta-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 44, no. 272, 31 October 1997 (1997-10-31), pages 27497 - 27500, XP002076354, ISSN: 0021-9258 *
BIERI CHRISTOPH ET AL: "Micropatterned immobilization of a G protein-coupled receptor and direct detection of G protein activation", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 11, November 1999 (1999-11-01), pages 1105 - 1108, XP002183971, ISSN: 1087-0156 *
GUREVICH VSEVOLOD V ET AL: "Visual arrestin binding to rhodopsin: Diverse functional roles of positively charged residues within the phosphorylation-recognition region of arrestin.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 11, 1995, pages 6010 - 6016, XP002187873, ISSN: 0021-9258 *
KOVOOR ABRAHAM ET AL: "Targeted construction of phosphorylation-independent beta-arrestin mutants with constitutive activity in cells.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 11, 12 March 1999 (1999-03-12), pages 6831 - 6834, XP002187872, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
AU2001249107A1 (en) 2001-09-17
WO2001067106A2 (en) 2001-09-13
WO2001067106A8 (en) 2002-02-14

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