+

WO2018138642A1 - Compositions nutraceutiques pour plantes et procédé de préparation associé - Google Patents

Compositions nutraceutiques pour plantes et procédé de préparation associé Download PDF

Info

Publication number
WO2018138642A1
WO2018138642A1 PCT/IB2018/050419 IB2018050419W WO2018138642A1 WO 2018138642 A1 WO2018138642 A1 WO 2018138642A1 IB 2018050419 W IB2018050419 W IB 2018050419W WO 2018138642 A1 WO2018138642 A1 WO 2018138642A1
Authority
WO
WIPO (PCT)
Prior art keywords
slurry
aqueous solution
salicylic acid
nutraceutical
composition
Prior art date
Application number
PCT/IB2018/050419
Other languages
English (en)
Inventor
Domenico Terenzio
Alessandro Bertolini
Original Assignee
Agri Tech World S.R.L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/IB2017/000013 external-priority patent/WO2017122082A1/fr
Application filed by Agri Tech World S.R.L filed Critical Agri Tech World S.R.L
Publication of WO2018138642A1 publication Critical patent/WO2018138642A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits

Definitions

  • Humic substances have critical functions in soil and plants. In soil, they bind to the elements present, particularly phosphorous and iron, thus promoting the bioavailability thereof towards the plant, avoiding the insolubilization due to the high pH and to the high content of active lime. They also promote the biodegradability of the toxins produced by plants and of all pollutants of organic origin brought to the soil or contained therein. They improve the chemical, physical and biological conditions of the soil by bringing organic carbon of biological origin, which play a catalytic role in promoting the absorption of nutrients by the plants. They also improve and homogenize the germination of seeds, have a strong rhizogenic action, i.e. promote the development of adventitious roots and enhance the biochemical activity of the soil and plants.
  • Humic substances play a very important complexing and carrier role, specifically of carboxylic and phenolic groups contained therein. In fact, the higher the content of organic carbon and its degree of humification, the stronger the complexing and carrier action they exert against the substances or elements to which they are associated.
  • Salicylic acid is an aromatic hydroxy acid found in plants, fruits and roots of Spiracea ulmaria , which belongs to the rosacea family, in hyacinths, tulips, strawberries and grapes. Salicylic acid is widely used in pharmaceuticals and cosmetics for its recognized anti-inflammatory, moisturizing, keratolytic action. In plants, salicylic acid activates the protein synthesis and participates in cellular destruction and SAR (Systemic Acquired Resistance) induction. This resistance is expressed against a wide range of harmful organisms and differs in its action according to the inductor pathogen agent. However, a use thereof on a large scale in agriculture has not yet become widespread, since a formulation which promotes the bioavailability thereof has not yet been developed.
  • SAR Systemic Acquired Resistance
  • Active silver nanoparticles are known for their antimicrobial activity against harmful organisms for plants, fungi, bacteria, viruses.
  • the present invention relates to a process and to nutraceutical compositions obtained by the same for the treatment of plants, said compositions having surprising nutritional and biostimulant properties and of enhancement of endogenous defenses of plants against fungi, bacteria and viruses. Said compositions also make plants more resistant to biotic infections, by limiting or avoiding the application of agrochemicals.
  • Figure 1 XPRD spectra (A) salicylic acid (reference standard) (B) composition A, (C) composition B, (D) composition C (comparative) .
  • Figure 2 ATR-FTIR spectra (A) salicylic acid (reference standard)
  • composition A composition A
  • composition B composition B
  • composition C composition B
  • FIG. 3 MR spectra (A) salicylic acid (reference standard) (B) composition A, (C) composition B, (D) composition C (comparative) .
  • A salicylic acid (reference standard)
  • B composition A
  • C composition B
  • D composition C (comparative)
  • fossil material denotes a material containing at least 80% w/w of total organic substance, at least 80% w/w of extractable organic substance and 80% w/w of humified organic substance.
  • the most advantageous fossil material has a total organic matter content of 90% w/w, with a percentage of extractability of the organic substance o f 90% and a degree of humification of 90%.
  • Fossil material is particularly rich in humic substances.
  • a first object of the present invention is a process for the preparation of nutraceuticals for plants which comprises:
  • a) Preparing a muddy mixture of fossil material by mixing said fossil material finely divided with an aqueous solution comprising AgNPs having a diameter of ⁇ 100 nm, preferably from 20 to 30 nm, with a concentration of silver in said aqueous solution from 20 ppm to 300 ppm;
  • step b) Adding salicylic acid to the mixture resulting from step a) with the mixture under stirring until complete homogenization of all the slurry;
  • step b) Adding an alkaline agent to the mixture of step b) under stirring, preferably selected from the group comprising potassium hydroxide, potassium carbonate, potassium bicarbonate, ammonium hydroxide or mixtures thereof.
  • an alkaline agent preferably selected from the group comprising potassium hydroxide, potassium carbonate, potassium bicarbonate, ammonium hydroxide or mixtures thereof.
  • the alkaline extraction allows the optimum extraction o f the humic substances contained in the fossil material.
  • said method also comprises, in said step c) , an ultrasonic treatment at very high power, approximately 80 kHz, at a temperature from 50 to 70 °C.
  • an ultrasonic treatment at very high power, approximately 80 kHz, at a temperature from 50 to 70 °C.
  • the exposure to ultrasounds allows an effective extraction, even in the presence o f low concentrations o f alkaline agent.
  • Said fossil material is preferably selected from the group comprising: leonardite, lignite, peat coal, xylite, peat and mixtures thereof, preferably leonardite.
  • said fossil material is finely ground and characterized by a particle size from 100 to 200 mesh, even more preferably from 100 to 150 mesh or from 120 to 150 mesh.
  • the fossil material and the aqueous solution are mixed in a ratio from about 1:0.1 to about 1:10, or from about
  • Said mixing ratios of the fossil material with the aqueous solution are also selected on the basis of the final formulation to be obtained, i.e. the ratios are in favor of the fossil material if a solid final formulation is desired.
  • salicylic acid is added in an amount from 1% to 10%, preferably from 3 to 7%, preferably it is about 5% or 3% w/w of the mixture .
  • the percentages of the alkaline agent added to the mixture are in the range from 3% to 7% w/w with respect to the mixture itself and in any case, their concentration is always proportional to the percentages of salicylic acid.
  • the slurry being worked which comprises said mixture of fossil material, salicylic acid, alkaline agent is kept under stirring for at least 1 hour, preferably for 2 hours, even more preferably for 5-6 hours. It is also possible to carry out said process with a continuous industrial plant.
  • the process described herein allows to obtain two basic compositions of nutraceuticals : an acidic composition at pH from 5.5 to 6.0 and an alkaline composition at pH from 9.5 to 12.00, depending on the percentages of salicylic acid used and alkaline agent used for the extraction of humic substances.
  • compositions for different productions/mixtures, for different soils (acidic or basic soils) and for different crops (acidophilus or basophilic crops) .
  • formulations suitable for hydroponic or soilless crops have been selected.
  • the slurry is optionally filtered (100-120 mesh) for the preparation of liquid formulations (at alkaline pH or acidic pH) , or dried using a (normally rotary) dryer and, optionally, granulated or microgranulated for precision applications on crops.
  • Microgranulation (0.4-1.0 mm) is recommended for solid products, since it allows a rational and precision distribution of the amounts of fertilizers actually added, a control which is required for sustainable agriculture.
  • Microgranular formulations can be distributed with precision machines, able to administer 25-40 kg/ha of formulation at the time of seeding, compared to 250-400 kg/ha dispensed nowadays with the current crop nutrition techniques .
  • said method of preparation comprises a first drying of the slurry carried out with a rotary dryer, leaving a residual moisture in the product of about 13-16%, preferably of about 15% which is followed by the granulation or microgranulation process, preferably with a disc granulator, which is followed by one final drying on a fluid bed plant.
  • the method comprising the drying in subsequent steps advantageously allows a better microgranulation or granulation, due to the residual moisture content still present during the granulation process.
  • a glue to the mixture, preferably of a natural origin, such as lignosul fonate or carboxymethylcellulose, has proved effective.
  • the formulation comprising the glue ensures a better hold of the granule and a better mechanical distribution of the granules, which is important in precision fertilization.
  • the production process according to the present invention may also be implemented in other industrial technologies that the process engineer will deem appropriate, depending on the specific needs, technologies allowing the introduction of the different raw materials in line while the plant is carrying out the homogenization of the whole slurry, up to obtain the titrated mixture already granulated or microgranulated .
  • the present production process comprises the necessary separation of the liquid moiety from the insoluble moiety of the mixtures, where the liquid phase represents the plant nutraceutical composition, which can be used as is or used to prepare mixtures of nutraceutics for plants with specific titrations.
  • a very fine, almost weightless fossil material ground to 120-150 mesh should be used in order to obtain better extraction and little filtration waste. It is also very important to carry out an adequate separation of the liquid moiety from the insoluble one in the mixture through a special filtering system, for example with filter press, vacuum filter and other systems available, avoiding the natural sedimentation technique, which also eliminates moieties which are very important from the agronomic point of view.
  • a reactor For the production of liquid formulations, at acidic pH or alkaline pH, according to the present invention, for direct use in agriculture or for use as an intermediate in the formulation of nutraceutics for plants, a reactor should be available, provided with high speed (2000-3000 rpm) stirring system, rpm control, preferably with two independent propeller stirring columns in order to allow to stir the slurry in both directions and stir the product on the bottom to the surface, and vice versa. This allows to obtain homogeneous productions and a better and complete extraction of humic substances.
  • the aqueous solution comprising AgNPs is added to the reactor, preferably of stainless steel, and then, the finely ground fossil material is added slowly under stirring.
  • the fossil material is introduced into the plant and the aqueous solution comprising AgNPs is added thereto.
  • the slow addition of the ground fossil material to the water already present in the reactor has proved particularly advantageous in order to obtain a homogeneous mixture without lumps.
  • salicylic acid is added slowly in the defined proportion, depending on the desired end product. It is kept under stirring for 2-3 hours. After 2-3 hours of stirring, the alkaline agent is poured slowly. The slurry is kept being worked for at least 1 hour, preferably for about 5-6 hours, after which the product is ready to be filtered and/or dried and/or stored in dedicated tanks.
  • the preparation process described herein leads to a product in which the complexing action of humic substances is maximized, so as to have a nutraceutical complex in which the bioavailability of active ingredients contained therein is maximized.
  • This particularly advantageous effect is achieved due to the specific combination of active ingredients and to the preparation process, which allows to maximize the effectiveness thereof .
  • compositions obtained with the process described above are described herein.
  • compositions were obtained which are used in organic farming, which comprise raw materials permitted and certified for use in organic farming, and compositions suitable for eco friendly agriculture comprising mineral and/or process raw materials only in very small amounts, so as not to have a strong environmental impact .
  • Liquid formulations, with acidic pH and alkaline pH, produced according to the present invention are advantageously used as an intermediate for the preparation of specific formulations, titered according to commercial and technical requirements.
  • Solid (granular and/or microgranular) formulations are also obtained with an acidic or alkaline pH, so as to adapt them to the crop and soils on which they are used.
  • Formulations with a high content of acidic pH humic acids are used in physiologically alkaline soils, in foliar applications, in hydroponics crops and in mixtures with agrochemicals.
  • Formulations with a high content of alkaline pH humic acid are used in acidic soils.
  • the formulations according to the present invention may further comprise one or more substances able to release macroelements, microelements and/or meso-elements .
  • Said macro-elements comprise, for example, nitrogen, phosphorus and potassium.
  • Said microelements comprise, for example, iron, zinc, manganese, copper, boron, molybdenum.
  • Said meso-elements comprise, for example, calcium and magnesium.
  • compositions further comprise the addition of animal or plant protein hydrolysates .
  • insect repellents fungicides, fungistatic agents, bactericidal agents, bacteriostatic agents, nematode repellents, plant growth regulators, complex fertilizers, adsorbentshas proved to be particularly effective.
  • said plant growth regulators are selected from cytokines, auxins, gibberellins .
  • said adsorbents are of natural origin and comprise starches and derivatives thereof, or they are synthesized and comprise potassium polyacrylates . Said adsorbents are preferably added in amounts from 5 to 20% w/w.
  • said method shall further comprises the addition of residues (cake) to said mixture from extraction processes of oils, preferably of neem [Azadirachta indica) , karanja ⁇ Pongamia glabra) , castor (Ricinus communis) , Jatropha (Jatropha curcas) .
  • compositions also comprise Sophora japonica extract.
  • the following table shows the results obtained in terms of average % increase in yields of productions, average % reductions of the fertilizing units administered and average reduction of the agrochemicals administered.
  • results in the single cultivations were obtained by applying the cultivation protocols described in detail in examples 13 to 32, using the formulations according to the present invention, as described in examples 1 to 12.
  • the results obtained on each of the cultivations are surprising in terms of yield increase, reduction of fertilizing units and agrochemicals administered.
  • the nutraceutrical formulation of example 12w was used the control of pests (insects, nematodes and mites), at the foliar doses of 4-5 kg/hectare and on specialized horticultural crops, provided with drip irrigation system, also administered to the soil at doses of 10-20 kg/hectare, carrying out 2 applications, one at 7-10 days post transplantation and the other halfway of the growing cycle.
  • pests insects, nematodes and mites
  • Table 1 effects obtained on the indicated crops using the cultivation protocols according to the present invention. production fertilizing agrochemicals yield units administered administered administered
  • the composition according to the present invention turned out to be surprisingly advantageous, showing a synergy between the different components.
  • a synergy made strong by the method used for the preparation of the composition, which allows the formation of a mixture of organic substances which comprises AgNPs and salicylic acid, from which the humic substance is then extracted.
  • the humic substance due to the chelating properties of its constituents, rich in phenolic and carboxylic groups, has a strong property as a carrier and complexing agent.
  • the extraction method described herein allows a nutraceutical complex with unique properties to be obtained, which allows maximum bioavailability of the components present.
  • the AgNPs show a fungicide, bactericide and antiviral action exceeding that expected when applied with other methods.
  • the combined presence of salicylic acid enhances the endogenous defenses of plants.
  • the advantage resulting from having the microgranulate formulations described herein, which allow precise control of the distribution of fertilizer, is also noted. These make the fertilization process eco- friendly, allowing the fertilizing units for the nutrition of crops to be rationalized.
  • the surprising activity observed in enhancing the endogenous defenses of plants also allows the drastic reduction of agrochemicals, thus preventing the use of substances with a very strong impact on the ecosystem and very high permanence in the environment and in agricultural products, as well as dangerous for the operators.
  • the advantages described are associates to a marked quality and quantity improvement of agricultural productions.
  • Examples 1 to 12 describe compositions and formulation processes
  • examples 13 to 32 describe cultivation protocols which involve the use of one or more of the compositions described in the examples above.
  • Example 1 nutraceutical for plants with a high content of humic acids and with acidic pH.
  • composition is described which is formulated as follows:
  • the formulation proved surprisingly advantageous in the preparation of nutraceuticals for plants, titered for example with macro-, micro-, meso-elements , nutritional catalysts, amino acids, seaweed extracts, plant extracts, protein hydrolysates .
  • the formulations also proved to be advantageous for use in hydroponic cultivations, for the preparation of nutrient solutions for any cultivation (flowers, vegetables, nurseries, etc%), for foliar applications and also as such for use in fertigation.
  • Example 2 nutraceutical for plants with a high content of humic acids and with alkaline pH.
  • composition is described which is formulated as follows:
  • composition proved to be surprisingly advantageous in the preparation of basic formulations and for use in acidic soils and on basophilic plants.
  • Example 3 preparation of a titered (5% organic nitrogen) nutraceutical for plants in liquid formulation.
  • the nutraceutical is prepared ng by mixing in proportion 1:1 or a formulation according to examples 1 or 2 and an animal or plant protein hydrolysate containing 10% (w/w) of 50% organic nitrogen.
  • Example 4 nutraceutical for plants titered in ammonia nitrogen (14%), copper (1%), zinc (4%), and manganese (2%), in liquid formulation .
  • the nutraceutical comprises:
  • Example 5 nutraceutical for plants, microgranular formulation (% are w/w in the finished granulate/microgranulate product)
  • the aqueous solution of silver nanoparticles is poured in a mixer.
  • Leonardite, neem cake, lithothamnium, garlic flour, castor cake, zinc sulfate, and salicylic acid are added slowly under stirring.
  • the mass is mixed continuously until it forms a homogeneous slurry.
  • potassium carbonate is then added to allow the extraction of humic substances from leonardite and form a stable and active complex.
  • the slurry is dried by means of a drying system, preferably rotary, so as to leave a residual humidity of 13-16%, ideal for granulation or, preferably, microgranulation of the product (0.4-1.0 mm) .
  • the product is dried again by means of a fluid bed dryer, so as not to damage the granules.
  • an adhesive of natural origin is added to the slurry, such as calcium lignosulfonate or carboxymethylcellulose .
  • the formulation comprising the glue ensures a better mechanical distribution of the granules, which is important in precision fertilization.
  • composition described herein has shown surprising advantages for use in organic farming, as it possesses a strong repellency activity against nematodes and soil insects, in addition to a strong rhizogenic action.
  • Example 6 nutraceutical for plants, microgranular formulation.
  • nanoparticles (20 ppm - 200 ppm) as
  • the aqueous solution of silver nanoparticles is poured in a mixer.
  • Leonardite, NP 12-54 monoammonium phosphate, zinc sulfate, manganese sulfate and salicylic acid are slowly added under stirring.
  • the mixture is homogeneously mixture up to form a homogeneous slurry, thereafter boron ethanolamine, technical urea and potassium hydroxide are slowly poured under stirring, so as to allow the extraction of the humic substances from leonardite and form a stable and active complex.
  • the slurry is dried by means of a drying system, preferably rotary, so as to leave a residual humidity of 13-16%, ideal for granulation or, preferably, microgranulation of the product (0.4- 1.0 mm) .
  • a drying system preferably rotary
  • the product is dried again by means of a fluid bed dryer, so as not to damage the granules .
  • an adhesive of natural origin is added to the slurry, such as calcium lignosulfonate or carboxymethylcellulose .
  • the formulation comprising the glue ensures a better mechanical distribution of the granules, which is important in precision fertilization.
  • Example 7 biological nutraceutical , WDG formulation, based on trace elements (copper, zinc and manganese)
  • the slurry is dried by means of a drying system, preferably rotary, so as to leave a residual humidity of 13-16%, ideal for microgranulation of the product (0.4-1.0 mm) .
  • a drying system preferably rotary
  • the product is dried again by means of a fluid bed dryer, so as not to damage the granules.
  • an adhesive of natural origin is added to the slurry, such as calcium lignosulfonate .
  • the formulation comprising the adhesive ensures the hold in the packaging and prevents the formation of powders during the handling by the operator.
  • Example 8 nutraceutical for plants, microgranular formulation.
  • the aqueous solution of silver nanoparticles is poured in a mixer and leonardite, glucono delta lactone, zinc sulfate, manganese sulfate, magnesium sulfate, ferrous sulfate, neem cake, guano and salicylic acid are slowly added under stirring.
  • the mixture is homogeneously mixture up to form a homogeneous slurry, thereafter boron ethanolamine, and potassium carbonate are slowly poured under stirring, so as to allow the extraction of the humic substances from leonardite and form a stable and active complex.
  • the slurry is dried by means of a drying system, preferably rotary, so as to leave a residual humidity of 13-16%, ideal for granulation or, preferably, microgranulation of the product (0.4-1.0 mm) .
  • a drying system preferably rotary
  • the product is dried again by means of a fluid bed dryer, so as not to damage the granules.
  • an adhesive of natural origin is added to the slurry, such as calcium lignosulfonate or carboxymethylcellulose.
  • the formulation is advantageously used in viticulture and horticulture and organic nursery.
  • Example 9 nutraceutical for plants characterized by high bio- stimulating action on the underground and off-ground apparatus.
  • spirulina Arthrospira platensis
  • a cyanobacterium very well known in the field of food supplements, characterized by a high protein content (60-70%) and, therefore, of organic nitrogen (10-11%), allows to obtain a formulation characterized by a marked biostimulant action enhancing the endogenous plant defenses, even in periods when the plant is subjected to strong biotic and abiotic stresses.
  • composition is described which is formulated as follows:
  • the product After the processing cycle, the product must be filtered to remove all insoluble substances. This can be done either with a vacuum filter or a filter press, with 100-120 mesh filter. Sedimentation filtration is not recommended s it also removes small useful fractions contained in the fossil material. This filtration method successfully applies also to the formulations described in examples 1 and 2.
  • Example 10 nutraceutical with a high content of urea nitrogen for eco friendly agriculture, for foliar and root application.
  • nutraceutical with a high content of humic acids, acidic pH
  • aqueous solution of active silver nanoparticles preferably with two propeller stirring columns and running direction switch is poured the aqueous solution of active silver nanoparticles and, with the system under stirring, glucono delta lactone is slowly added followed by technical urea.
  • the nutraceutical with a high content of humic acids of example n. 1 and the animal or plant protein hydrolyzate are added.
  • the slurry is kept under stirring for about one hour, after which the product is ready to be packaged or stored in dedicated tanks.
  • Potassium bicarbonate, glucono delta lactone and the oligosaccharide are poured in a mixer.
  • the whole mass is homogenized and, while the system is under stirring, the nutraceutical with a high content of humic acids at acidic pH is slowly poured so as to form a semi-hard muddy mixture (slurry) .
  • Said slurry is dried in a rotary dryer to maintain a humidity of 12-13% to allow a regular microgranulation (0.4-0.6 mm) .
  • Microgranulation is carried out with the aid of natural adhesive substances, such as calcium lignosulfonate so as to obtain an intact granule during the agricultural operator's manipulation and already before, during the industrial packaging process.
  • Said formulation allows to bring a high content of potassium to the plants and make them more resistant to fungal infections, such as Botrytis, sclerotinia and powdery mildew.
  • All the extracts are gradually poured, one at a time, a zirconium microsphere mill provided with speed variator leaving the system under stirring for at least 20 minutes, for every single raw material.
  • An operating speed of 500 rpm is preferable.
  • the nutraceutical with a high content of humic acids and the oligosaccharide are added in the end, leaving the entire slurry under stirring for about 60 minutes at a speed of 500 rpm.
  • Preparative example 12b nutraceutical for plants containing complexed phosphites, characterized by high performance on plants, for applications on the foliar apparatus and soil fertigation.
  • composition is described which is formulated as follows:
  • nutraceutical with a high content of humic acids and acidic pH.
  • aqueous solution containing AgNPs, salicylic acid and phosphorous acid under stirring and stirring is continued for 30 minutes, then ammonium hydrate is slowly added and stirred for about three hours. Copper hydroxide is added and stirred for about one hour.
  • nutraceutical with a high content of humic acids, acidic pH is slowly added and kept under stirring for 5-6 hours .
  • the processed product is allowed to cool and then it is filtered at 120 mesh and packaged or stored in special tanks provided with pre-picking stirring system.
  • nutraceutical formulation has proved surprisingly advantageous to bring phosphorus to plants in a particularly bioavailable and fast formula (P0 3 ) , in addition to nitrogen in ammonia form and copper as trace element, especially useful in numerous biochemical activities of plants.
  • said nutraceutical preparation is a potent inducer of resistance in plants, particularly effective for the prevention of fungal diseases, particularly those induced by Oomycetes such as Peronospora spp. and Bremia spp. for bacterial diseases.
  • the effectiveness of the nutraceutical preparation is due to the synergistic action of the salicylic acid with the phosphorous acid and the silver nanoparticles , complexed by the humic substances present in the nutraceutical with a high content of humic acids, in which the carboxylic and phenol acids are present, characterized by a strong complexing and carrier action.
  • Said nutraceutical preparation represents an important innovation in the sector of phosphites, since it allows to have a highly bioavailable and effective preparation and at the same time with a low content of phosphites and phosphorous acid, with respect to the formulations currently available on the international market.
  • Example 13 cultivation protocol of salad, leaf lettuce, arugula, celery and valerian, in protected cultivation.
  • An application to the soil of a product of example 8 is carried out prior to seeding or transplantation at the dose of 500 kg/hectare .
  • an application is carried, by sprinkling or by means of localized fertigation, of the product of example 4, at the dose of 20-30 kg/hectare (the highest dose is applied for the distribution by sprinkling over the entire surface) .
  • an application of the formulation of example 10 is carried out in localized fertigation or sprinkling.
  • Example 14 cultivation protocol of tomato, pepper and eggplant, in protected cultivation.
  • example 8 The formulation of example 8 at the dose of 300 kg/hectare was applied pre-transplantation, localized on the rows. This is a slow-release formulation, which allows to obtain a balanced plant with a strong and expanded root system.
  • example 1 Immediately after transplantation, the formulation of example 1 was distributed in localized fertigation at doses of 25 kg/hectare .
  • example 7 The formulation of example 7, at a dose of 4 kg/hectare, was used 7 days after the transplantation, for the entire cultivation cycle at intervals of 7 days.
  • example 4 was used in localized fertigation at a dose of 25 kg/hectare 21 days and 45 days post transplantation. Said formulation allows to enhance the endogenous defenses of plants against fungi and bacteria, in addition to having a rhizogenic and nutritional action.
  • the protocol can significantly reduce the nitrogen units used and thus the permanence thereof in vegetables and aquifers.
  • example 7 Since the early vegetative stages and for the entire cultivation cycle, the formulation of example 7 was applied at a dose of 5 kg/hectare by foliar spraying, at intervals of 10 days.
  • a foliar application was carried out halfway of the cultivation cycle with the formulation of example 4, at a dose of 5 kg/hectare .
  • Example 16 Cultivation protocol for bean and string bean
  • the product of example 7 was applied at a dose of 4 kg/hectare by foliar spraying, at intervals of 8 days.
  • Example 17 Cultivation protocol for Cannabis sativa
  • a treatment with 200-300 kg/hectare of the nutraceutical of example 8 is carried out upon transplantation of the cultivation.
  • the nutraceutical of example 12 is administered at 10-14 day intervals, with foliar applications, at a dose of 5 kg/hectare, mixed with the nutraceutical of example 12, at a dose of 5 kg/hectare.
  • the nutraceutical of example 9 is administered at the dose of 5 kg/hectare pre-blooming and post- fruiting by foliar applications. After post- fruiting, the nutraceutical formulation of example 3 is applied in localized fertigation, at the dose of 25 kg/hectare, admixed with 10 kg/hectare of the nutraceutical of example 11.
  • the nutraceutical of example 11 is administered in foliar applications in the last 45 days of the cultivation cycle, at a dose of 5 kg/hectare, in three applications at 14-day intervals.
  • the protocol allows to prevent fungal and bacterial diseases on cannabis, avoid damage by pests and, very importantly, obtain an increase in the content of cannabinoids, especially THC (delta-9- tetrahydrocannabinol ) even with peaks up to 35%.
  • Example 18 Cultivation protocol for cucumber, watermelon and melon
  • example 8 at the dose of 150 kg/hectare was applied upon transplantation localized on the rows.
  • example 7 Since the early vegetative stages and for the entire cultivation cycle, the formulation of example 7 was applied at a dose of 5 kg/hectare by foliar spraying, at intervals of 7 days. In the fruit growing phase, the formulation of example 10 was used at a dose of 30 kg/hectare for 4 applications, at 10-day intervals .
  • the formulation of example 4 was used in localized fertigation at a dose of 20 kg/hectare 15 days and 45 days post transplantation. During the cultivation (about halfway of the cultivation cycle), the formulation of example 11 was applied three times at a dose of 5 kg/hectare, at intervals of 12 days.
  • example 8 Prior to seeding, the formulation of example 8 was administered by broadcast seeding over the entire surface, at the dose of 200 kg/hectare .
  • example 4 Immediately after seeding, the formulation of example 4 at the dose of 30 kg/hectare was applied over the entire surface by sprinkling .
  • example 7 The formulation of example 7, at a dose of 5 kg/hectare, was used since the early vegetative stages and for the entire cultivation cycle at intervals of 7 days.
  • example 11 was applied on the cultivation by foliar spraying at the dose of 5 kg/hectare .
  • example 8 at the dose of 200 kg/hectare was applied upon seeding, localized on the rows.
  • example 7 Since the early vegetative stages and for the entire cultivation cycle, the formulation of example 7 was applied at a dose of 5 kg/hectare by foliar applications, at weekly intervals.
  • example 10 was used in spraying fertigation at a dose of 35 kg/hectare halfway of the cultivation cycle.
  • example 11 was applied in the last month of the cultivation cycle by foliar spraying at the dose of 5 kg/hectare, with two applications at intervals of 12 days.
  • Example 21 Cultivation protocol for strawberry The formulation of example 8 at the dose of 200 kg/hectare was applied upon transplantation, localized on the convexing.
  • example 1 Immediately after transplantation, the formulation of example 1 was administered by localized fertigation at doses of 30 kg/hectare .
  • example 4 The formulation of example 4, at a dose of 20 kg/hectare, was administered every 30 days for the entire cultivation cycle by localized fertigation.
  • example 7 Since the early vegetative stages, the formulation of example 7 was administered at a dose of 5 kg/hectare by foliar spraying, at intervals of 10 days, for the entire vegetative cycle.
  • example 10 was administered by localized fertigation at a dose of 30 kg/hectare at 12 day intervals.
  • Example 22 Cultivation protocol for wheat, corn, barley, sunflower seeds, quinoa, soy, rapeseeds, peanuts.
  • example 6 Upon seeding, the formulation of example 6 was administered close to seeds at the dose of 40 kg/hectare.
  • example 11 at the dose of 6 kg/hectare was applied to the cultivation one month prior to harvesting.
  • example 6 was applied pre-seeding, localized on the rows, at the dose of 200 kg/hectare.
  • Example 25 Cultivation protocol for tobacco
  • example 8 was applied upon transplantation, localized on the rows, at the dose of 80 kg/hectare.
  • example 11 was administered one month prior to harvesting the leaves, at a dose of 5 kg/hectare for 2 applications, at 12-day intervals.
  • Example 26 Cultivation protocol for plum, peach, cherry, apricot and kiwi
  • foliar treatment with the formulation of example 4, at a dose of 8 kg/hectare, preferably in 1000 liters of water/hectare .
  • Example 28 Cultivation protocol for banana and and plantain.
  • foliar treatments with the formulation of example 4, at a dose of 4 kg/hectare, also with aerial means, at intervals of 12-14 days.
  • Example 29 Cultivation program for papaya, mango, pineapple, coffee and cocoa.
  • Example 30 Cultivation program for pomegranate, blackberry, raspberry, blueberry.
  • Example 31 Cultivation protocol for grapevine.
  • Example 32 Cultivation protocol of tea.
  • Foliar applications at 10 day intervals, with the formulation of example 10, at a dose of 6 kg/hectare, alternated with the application of the formulation containing spirulina of example 9 at a dose of 4 kg/hectare.
  • Example 33 Physical chemical characterization of compositions A and B according to the present description and composition C (comparative) .
  • compositions were prepared: A (according to the invention) , salicylic acid 8% w/w
  • composition was prepared as follows:
  • composition was prepared as follows:
  • composition was prepared as follows:
  • compositions A, B and C were tested by MR (Nuclear magnetic resonance spectroscopy), PXRD (Powder X-Ray Diffraction) and ATR-FTIR (Attenuated Total Reflection) .
  • the samples were lyophilized before analysis. Approximately 25 ml of each of the samples A, B, C were taken after vigorously shaking them. The suspension was transferred into a flask, brought to the liquid nitrogen temperature and set to lyophilize for 3 days. The samples thus treated have a dry powder appearance and are ready for subsequent analysis.
  • the presence or absence of salicylic acid in the formulations was carried out using the MR spectroscopy in solution.
  • For each sample and for the reference salicylic acid about 10 mg of substance are weighed.
  • the powder was transferred into a test tube with magnetic stirrer, suspended in 1 ml of deuterated water (D20) and left under magnetic stirring for 2 h. Microfiltration was then carried out and the filtrate was transferred to a 5 mm NMR tube.
  • the 1H NMR spectra were acquired on a Bruker Avance 500 NMR spectrometer with 11.4 Tesla magnet. The residual water signal at 4.7 ppm was suppressed / attenuated using the 1D-NOESY sequence.
  • the X-ray diffraction spectra from powders were performed on a Bruker diffractometer D2 Phaser using CuKa radiation. Data were collected in a range 2 ⁇ from 2.3 to 60° with the following parameters: step size 0.02°, counting time 0.4 s per step, primary slit module 0.6 mm, air scatter screen module equal to 1 mm and secondary slit module of 8 mm.
  • the ATR-FTIR spectra were acquired with a Varian 640 IR spectrophotometer.
  • a ZnSe crystal For the ATR mode used a ZnSe crystal.
  • the spectra were acquired from 4000 to 400 cm-1 and averaged over 64 acquisitions, with a resolution of 4 cm-1.
  • Salicylic acid (Figure 2A) has three distinctive bands in the fingerprint region to be used as markers: 756 cm-1, 691 cm-1 and 657 cm- 1.
  • Samples A and B show two absorptions at 746 cm-1, tentatively assignable to salicylic acid, also considering the results of NMR spectra that unequivocally attest to the presence of acid in these two formulations. Assuming correct assignment the frequency shift of the band from 756 to 746 cm-1 could be interpreted as a significant interaction of salicylic acid with matrix in the case of the formulations A and B according to the present invention.
  • Sample C clearly shows a 759 cm-1 band, consisting of the marker of salicylic acid.
  • the acid salicylic does not show variations in frequency, thus attesting its presence as a component independent in a physical mixture.
  • formulations A and B according to the present invention clearly show a salicylic acid amphization process, absent in sample C. This process is complete in the formulation A and extended, though not complete, in the formulation B.
  • bioavailability of an active ingredient for example a pharmaceutical component is directly related to the crystallinity : to increase the bioavailability it is necessary to move towards an amorphous formulation of API.
  • compositions A and B the totally or almost totally amorphous character of salicylic acid is preserved after freeze- drying .

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un procédé et des compositions nutraceutiques obtenues par la mise en oeuvre dudit procédé, pour le traitement de plantes, lesdites compositions étant dotées de propriétés nutritionnelles et biostimulantes étonnantes tout en améliorant les défenses endogènes des plantes contre les champignons, les bactéries et les virus. Les compositions selon l'invention rendent également les plantes plus résistantes aux infections biotiques, en ce qu'elles permettent de limiter ou d'éviter l'application de produits agrochimiques.
PCT/IB2018/050419 2017-01-12 2018-01-24 Compositions nutraceutiques pour plantes et procédé de préparation associé WO2018138642A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/IB2017/000013 WO2017122082A1 (fr) 2016-01-14 2017-01-12 Élément d'espacement pour coffrage jetable pour la construction de murs et coffrage jetable incorporant l'élément d'espacement
IBPCT/IB2017/000013 2017-01-24

Publications (1)

Publication Number Publication Date
WO2018138642A1 true WO2018138642A1 (fr) 2018-08-02

Family

ID=61094561

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2018/050419 WO2018138642A1 (fr) 2017-01-12 2018-01-24 Compositions nutraceutiques pour plantes et procédé de préparation associé

Country Status (1)

Country Link
WO (1) WO2018138642A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113693088A (zh) * 2021-08-27 2021-11-26 上海太尘新型材料科技有限公司 一种环保型埃米生物提取组合物、其应用及其制备方法
CN115093293A (zh) * 2022-05-30 2022-09-23 中国农业大学 一种苹果树专用套餐肥

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003051120A1 (fr) * 2001-12-13 2003-06-26 Morse Enterprises Limited, Inc. Preparations stabilisees concentrees renforçant les reponses defensives de plantes
RU2255924C1 (ru) * 2003-12-24 2005-07-10 Закрытое акционерное общество "Сельскохозяйственное предприятие Озерское" (ЗАО СХП "Озерское") Способ получения жидкого органоминерального удобрения из хитозансодержащего сырья
US20080221314A1 (en) * 2007-03-07 2008-09-11 Timac Agro Espana, S.A. Heteromolecular metal-humic (chelate) complexes
KR20090051923A (ko) * 2007-11-20 2009-05-25 박정림 항균성이 증대된 식물 질병 방제용 은나노 조성물 및 그제조방법
US20140179520A1 (en) * 2012-12-21 2014-06-26 Brandt Consolidated, Inc. Humic Acid Composition

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003051120A1 (fr) * 2001-12-13 2003-06-26 Morse Enterprises Limited, Inc. Preparations stabilisees concentrees renforçant les reponses defensives de plantes
RU2255924C1 (ru) * 2003-12-24 2005-07-10 Закрытое акционерное общество "Сельскохозяйственное предприятие Озерское" (ЗАО СХП "Озерское") Способ получения жидкого органоминерального удобрения из хитозансодержащего сырья
US20080221314A1 (en) * 2007-03-07 2008-09-11 Timac Agro Espana, S.A. Heteromolecular metal-humic (chelate) complexes
KR20090051923A (ko) * 2007-11-20 2009-05-25 박정림 항균성이 증대된 식물 질병 방제용 은나노 조성물 및 그제조방법
US20140179520A1 (en) * 2012-12-21 2014-06-26 Brandt Consolidated, Inc. Humic Acid Composition

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 200552, Derwent World Patents Index; AN 2005-510646, XP002780527 *
DATABASE WPI Week 200971, Derwent World Patents Index; AN 2009-K23178, XP002780526 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113693088A (zh) * 2021-08-27 2021-11-26 上海太尘新型材料科技有限公司 一种环保型埃米生物提取组合物、其应用及其制备方法
CN115093293A (zh) * 2022-05-30 2022-09-23 中国农业大学 一种苹果树专用套餐肥

Similar Documents

Publication Publication Date Title
US10301229B2 (en) Process for the preparation of humic extracts from fossil sources
KR100866587B1 (ko) 게르마늄 및 셀레늄 함유 액상비료의 제조방법, 이로부터제조된 액상비료 및 이를 이용한 작물의 재배방법
Arthur et al. Vermicompost leachate alleviates deficiency of phosphorus and potassium in tomato seedlings
US20180077938A1 (en) Process to elaborate a biostimulant based on seaweeds
KR20130092275A (ko) 유황비료 제조방법 및 이에 의해 제조된 유황비료
RU2490241C1 (ru) Органо-минеральное комплексное удобрение и способ его получения
Shafique et al. Efficiency of cow dung based vermi-compost on seed germination and plant growth parameters of Tagetes erectus (Marigold)
KR101225890B1 (ko) 해조류를 이용한 기능성 강화 액체비료 및 그 제조방법
KR101117864B1 (ko) 고철분 및 고칼슘 쌀 그리고 벼 재배방법
WO2018138642A1 (fr) Compositions nutraceutiques pour plantes et procédé de préparation associé
KR100524116B1 (ko) 해조추출물, 부식산, 아미노산, 지베렐린 및 사이토키닌을함유하는 복합 비료 및 그 제조방법
KR102642336B1 (ko) 금속 및 아스코르브산 복합체의 제형, 이의 수득 및 용도
Nazir et al. Effect of integrated organic nutrient management on fruit yield and quality of strawberry cv Senga Sengana
WO2018138742A1 (fr) Compositions nutraceutiques pour plantes et procédé de préparation associé
RU2109446C1 (ru) Способ получения натурального регулятора роста растений
KR20050072654A (ko) 유기게르마늄을 함유한 농산물 생산 농법
KR102550898B1 (ko) 이탄을 유효성분으로 포함하는 유기질 토양개량제 및 이의 용도
Shankar et al. Effect of sea weed extracts on the growth, yield attribute and nutrient uptake of sesame (Sesamum indicum L.)
Laware et al. Effect of organically chelated micronutrients on growth and productivity in okra
KR20220163167A (ko) 동·식물용 셀레늄 영양제의 제조방법
JP6998256B2 (ja) タマネギ中のケルセチン増量剤およびタマネギの栽培方法
Sridhar et al. The effects of Seaweed Liquid Fertilizer of Ulva lactuca on Capsicum annum
Souri et al. Benefits of organic fertilizer spray on growth quality of chili pepper seedlings under cool temperature.
RU2137738C1 (ru) Органическая смесь для питания растений и способ ее получения
Kumar et al. Effect of different sources of organic manure granules on the growth of hybrid maize.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18702343

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18702343

Country of ref document: EP

Kind code of ref document: A1

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载