WO2018170561A1 - Biorremediate process with bacterial, fungic and enzymatic having substrate a midst constituted by cellulose material - Google Patents
Biorremediate process with bacterial, fungic and enzymatic having substrate a midst constituted by cellulose material Download PDFInfo
- Publication number
- WO2018170561A1 WO2018170561A1 PCT/BR2017/000023 BR2017000023W WO2018170561A1 WO 2018170561 A1 WO2018170561 A1 WO 2018170561A1 BR 2017000023 W BR2017000023 W BR 2017000023W WO 2018170561 A1 WO2018170561 A1 WO 2018170561A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- substrate
- midst
- cellulose
- constituted
- constitute
- Prior art date
Links
- 239000000463 material Substances 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims description 13
- 229920002678 cellulose Polymers 0.000 title claims description 12
- 239000001913 cellulose Substances 0.000 title claims description 12
- 239000000758 substrate Substances 0.000 title claims description 11
- 230000001580 bacterial effect Effects 0.000 title claims description 5
- 230000002255 enzymatic effect Effects 0.000 title claims description 5
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 9
- 241000233866 Fungi Species 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000002689 soil Substances 0.000 claims abstract description 6
- 230000014621 translational initiation Effects 0.000 claims abstract description 4
- 239000002131 composite material Substances 0.000 claims abstract description 3
- 230000004048 modification Effects 0.000 claims abstract description 3
- 238000012986 modification Methods 0.000 claims abstract description 3
- 239000003795 chemical substances by application Substances 0.000 claims abstract 2
- 238000000227 grinding Methods 0.000 claims description 5
- 238000006065 biodegradation reaction Methods 0.000 claims description 4
- 231100000252 nontoxic Toxicity 0.000 claims description 4
- 230000003000 nontoxic effect Effects 0.000 claims description 4
- 230000001131 transforming effect Effects 0.000 claims description 4
- SNSBQRXQYMXFJZ-MOKYGWKMSA-N (2s)-6-amino-n-[(2s,3s)-1-amino-3-methyl-1-oxopentan-2-yl]-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-amino-3-phenylpropanoyl]amino]-3-hydroxypropanoyl]amino]propanoyl]amino]-3-hydroxypropanoyl]amino]propanoyl]amino]-4-methylpentanoy Chemical compound CC[C@H](C)[C@@H](C(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC1=CC=CC=C1 SNSBQRXQYMXFJZ-MOKYGWKMSA-N 0.000 claims description 3
- 239000003463 adsorbent Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 210000000056 organ Anatomy 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000013467 fragmentation Methods 0.000 claims description 2
- 238000006062 fragmentation reaction Methods 0.000 claims description 2
- 239000003415 peat Substances 0.000 claims description 2
- 238000013138 pruning Methods 0.000 claims description 2
- 238000001179 sorption measurement Methods 0.000 claims description 2
- 238000003786 synthesis reaction Methods 0.000 claims description 2
- 241000771208 Buchanania arborescens Species 0.000 claims 1
- 229920005615 natural polymer Polymers 0.000 claims 1
- 238000000643 oven drying Methods 0.000 claims 1
- 239000000356 contaminant Substances 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 239000003344 environmental pollutant Substances 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 229920005601 base polymer Polymers 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000011122 softwood Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/286—Treatment of water, waste water, or sewage by sorption using natural organic sorbents or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/68—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water
- C02F1/681—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water by addition of solid materials for removing an oily layer on water
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/40—Valorisation of by-products of wastewater, sewage or sludge processing
Definitions
- the present application for patent refers to an innovative product developed from the use of bacteria, fuirigi and enzymes for the bio-polymerization of a specific midst with adsorbent/biodegradation characteristics using as substrate a means constituted of materials composed of cellulose where there is a reaction in synthesis creating conditions of expressiveness for the bioremediation of carboniferous moiecuies.
- the leaching process (liberation of the initially absorbed oils), causing the return of pollutants to the environment and when in the soil prevents the mechanical action of the oils in the processes of gas exchange between the free 0 2 and ground substrate.
- the use of naturally polymeric bacteria, fungi arid enzymes using as substrate a midst constituted of materials composed of cellulose demonstrate significant advantages over the above listed products, because when it is of interest of the recovery of the oily substances it has the property of transforming them in a very short time, a totally non-toxic compound info the means by the action of said enzymes in this specific substrate (by its characteristics promotes an action of absorbency in the carboniferous molecules realizing its addition to its environment and eliminating immediately the aggressive characteristics to the ecosystem), of lower density than water (Even when associated with oils) And for being chemically non-polar by its vegetal characteristic (Suffering total rejection by the water) ensure an good buoyancy, preventing by these factors the reintroduction of the polluting element to the water environment.
- the production technology of the present invention has four distinct phases, namely:
- Oxirredutases, Hydrolases, Ligases and Isomerases on culture basis;
- BIORREMEDIATE PROCESS WITH BACTERIAL, FUNGIC AND ENZYMATIC HAVING SUBSTRATE A MIDST CONSTITUTED BY CELLULOSE MATERIAL obviousness an innovative product developed from the use of bacteria, fungi and enzymes for bio-polymerization of a specific midst with adsorbent/biodegradation characteristics for the adsorption/bioremediation of carboniferous liquid substances of the environment in water, soil, with superior efficiency technologies and existing products.
- Being its substrate conceived in granular form promoting the addition to its means of oily liquid contaminants with a reason of the weight in the range among 100% to 200%.
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Soil Sciences (AREA)
- Biotechnology (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a product developed from the use of bacteria, fungi and enzymes for biopolymerization of a specific medium constituted of celluiosic material. The action of bacteria, fungi and enzymes leads to structural modifications of the celluiosic material, resulting in a material with high fioatability degree and ability to adsorb contaminants. Consequently, this material can be used for the bioremediation of carbonaceous molecules in liquid substances and in soils, where the polluting agent is transformed into an composite totally non¬ toxic to the environment.
Description
"BIORREMEDIATE PROCESS WITH BACTERIAL, FUNGIC AND ENZYMATIC HAVING SUBSTRATE A MIDST CONSTITUTED BY CELLULOSE MATERIAL".
DESCRIPTION
The present application for patent refers to an innovative product developed from the use of bacteria, fuirigi and enzymes for the bio-polymerization of a specific midst with adsorbent/biodegradation characteristics using as substrate a means constituted of materials composed of cellulose where there is a reaction in synthesis creating conditions of expressiveness for the bioremediation of carboniferous moiecuies.
The necessity of this development becomes evident, when we face a reality of permanent risk to the environment owing to pollution by liquid substances containing carboniferous moiecuies caused by human failures or by breakdown of systems and equipment.
The products currently used for the absorption of liquid carbonic substances from the environment when accidents happen, are the use of materials with absorption power.
However many of these materials have the drawback of substantially increasing the volume of waste function of its non-biodegradation or by a slow process. Sometimes, causing greater damage to the environment causing in some cases (as in water) the sinking of the oil owing to increase their densities and/or affinity for water environment.
Also, occurring in certain cases, the leaching process (liberation of the initially absorbed oils), causing the return of pollutants to the environment and when in the soil prevents the mechanical action of the oils in the processes of gas exchange between the free 02 and ground substrate.
The use of naturally polymeric bacteria, fungi arid enzymes using as substrate a midst constituted of materials composed of cellulose demonstrate significant advantages over the above listed products, because when it is of interest of the recovery of the oily substances it has the property of transforming them in a very short time, a totally non-toxic compound info the means by the action of said enzymes in this specific substrate (by its characteristics promotes an action of absorbency in the carboniferous molecules realizing its addition to its environment and eliminating immediately the aggressive characteristics to the ecosystem), of lower density than water (Even when associated with oils) And for being chemically non-polar by its vegetal characteristic (Suffering total rejection by the water) ensure an good buoyancy, preventing by these factors the reintroduction of the polluting element to the water environment. When in the soil it prevents the mechanical action of the oils in the processes of gas exchange and when in the soil it prevents the mechanical action of the oils in the processes of gas exchange between the free O* and the terrestrial environment when the pollutant is still in the contaminating state and transforming it into an organ composite totally non-toxic to the environment.
The production technology of the present invention has four distinct phases, namely:
1) Process drying/lyophilization substrate - This phase is preceded by drying materials based on cellulose (derived from softwoods and pruning residues of peat);
2) Grinding (fragmentation) of the material - This phase is preceded grinding the cellulose-based material in granularity between 3.0 / 8.0mm;
3) A process of adding bacteria and fungi biodecompositores - Alter grinding of the components adding suitable percentage (2% to 5%) of Tioxidans and oxidizing bacteria of the sort. Thiobaciilus Ferroxidans, Thiobacii!us Thiooxidans, SC27;
4) Addition in pre-defined proportions of natural base polymer enzymes -
Specific doses of: Oxirredutases, Hydrolases, Ligases and Isomerases on culture basis;
5) Final processing - Structural physical modification of the product result of enzyme addition to the polymeric substrate.
The object of the present Application for Patent Privilege of Invention, entitled BIORREMEDIATE PROCESS WITH BACTERIAL, FUNGIC AND ENZYMATIC HAVING SUBSTRATE A MIDST CONSTITUTED BY CELLULOSE MATERIAL obviousness an innovative product developed from the use of bacteria, fungi and enzymes for bio-polymerization of a specific midst with adsorbent/biodegradation characteristics for the adsorption/bioremediation of carboniferous liquid substances of the environment in water, soil, with superior efficiency technologies and existing products. Being its substrate conceived in granular form promoting the addition to its means of oily liquid contaminants with a reason of the weight in the range among 100% to 200%. That by the characteristics of its basic substances does not present any degree of toxicity, High degree of buoyancy, and transforming the pollutant into a completely nontoxic organ compound. In the present application we come up with the technological innovation of manipulation of the bacterial, fungal and enzymatic elements, using as substratum a midst constituted by materials composed of cellulose using them of form and specific purpose.
Claims
CLAIM
"B!ORREMEDIATE PROCESS WITH BACTERIAL, FUNGiC AND ENZYMATIC HAVING SUBSTRATE A MIDST CONSTITUTED BY CELLULOSE MATERIAL".
the present Application for Patent Privilege of Invention refers to an innovative product developed from the use of bacteria, fungi and enzymes for bio- polymerization of a specific midst with adsorbent/biodegradation characteristics for the adsorption/bioremediation using as substrate a midst constituted of materials composed of cellulose where there is a reaction in synthesis creating conditions of expressiveness for the bioremediation of carboniferous molecules in liquid substances and in soils transforming the polluting agent into an organ composite totally non-toxic to the environment.
Patent claim 1: Constitute a process of total drying (75%) of the substrate. When it is made from the oven drying of materials based on cellulose (light woods derived from pruning residues and peat);
Patent claim 2: Constitute a process of grinding (fragmentation) of the material. When grinding the cellulose based material in granularity between 1.0 / 3.0 mm;
Patent claim 3: Constitute the addition of pre-defined proportions of bacteria, fungi and enzymes natural polymer based on specific doses;
Patent claim 4: Constitute the final processing of structural physical modification of the product result of enzyme addition to the polymeric substrate
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/BR2017/000023 WO2018170561A1 (en) | 2017-03-22 | 2017-03-22 | Biorremediate process with bacterial, fungic and enzymatic having substrate a midst constituted by cellulose material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/BR2017/000023 WO2018170561A1 (en) | 2017-03-22 | 2017-03-22 | Biorremediate process with bacterial, fungic and enzymatic having substrate a midst constituted by cellulose material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2018170561A1 true WO2018170561A1 (en) | 2018-09-27 |
Family
ID=63583866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/BR2017/000023 WO2018170561A1 (en) | 2017-03-22 | 2017-03-22 | Biorremediate process with bacterial, fungic and enzymatic having substrate a midst constituted by cellulose material |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2018170561A1 (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0594125A2 (en) * | 1992-10-20 | 1994-04-27 | Canon Kabushiki Kaisha | Carrier for supporting microorganisms, soil remediating agent using carrier, and method for remediating soil |
| WO1995026944A1 (en) * | 1994-03-30 | 1995-10-12 | Theodore Dickerson | Process and material for bioremediation of hydrocarbon contaminated soils |
| US5656490A (en) * | 1993-07-23 | 1997-08-12 | Wyatt; Caryl Heintz | Enzyme compositions and methods for biodegradation separation of natural fibers and adsorbed petroleum products |
| US5738703A (en) * | 1993-04-06 | 1998-04-14 | Bandurski; William E. | Method to produce a substitute for peat moss |
| US5821113A (en) * | 1993-05-25 | 1998-10-13 | Envorflow Inc. | Method of reducing contamination and composition for use in the method |
| BRPI0703623A2 (en) * | 2007-10-03 | 2009-05-19 | Luiz Antonio Alves Pereira | improved system and manufacturing process of bioremediation product of liquid substances containing carbon molecules in their structure |
| WO2012008841A1 (en) * | 2010-07-14 | 2012-01-19 | Ellingsen, Hans Falnes | Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture |
-
2017
- 2017-03-22 WO PCT/BR2017/000023 patent/WO2018170561A1/en active Application Filing
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0594125A2 (en) * | 1992-10-20 | 1994-04-27 | Canon Kabushiki Kaisha | Carrier for supporting microorganisms, soil remediating agent using carrier, and method for remediating soil |
| US5738703A (en) * | 1993-04-06 | 1998-04-14 | Bandurski; William E. | Method to produce a substitute for peat moss |
| US5821113A (en) * | 1993-05-25 | 1998-10-13 | Envorflow Inc. | Method of reducing contamination and composition for use in the method |
| US5656490A (en) * | 1993-07-23 | 1997-08-12 | Wyatt; Caryl Heintz | Enzyme compositions and methods for biodegradation separation of natural fibers and adsorbed petroleum products |
| WO1995026944A1 (en) * | 1994-03-30 | 1995-10-12 | Theodore Dickerson | Process and material for bioremediation of hydrocarbon contaminated soils |
| BRPI0703623A2 (en) * | 2007-10-03 | 2009-05-19 | Luiz Antonio Alves Pereira | improved system and manufacturing process of bioremediation product of liquid substances containing carbon molecules in their structure |
| WO2012008841A1 (en) * | 2010-07-14 | 2012-01-19 | Ellingsen, Hans Falnes | Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture |
Non-Patent Citations (4)
| Title |
|---|
| BAYAT, Z. ET AL.: "Immobilization of Microbes for Bioremediation of Crude Oil Polluted Environments: A Mini Review", OPEN MICROBIOL J., vol. 9, 2015, pages 48 - 54, XP055550644 * |
| DZIONEK, A. ET AL.: "Natural carriers in bioremediation: A review", ELECTRONIC JOURNAL OF BIOTECHNOLOGY, vol. 23, 2016, pages 28 - 36, XP055550642, Retrieved from the Internet <URL:https://doi.org/10.1016/j.ejbt.2016.07.003> * |
| KUMAR, A. K. ET AL.: "Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review", BIORESOURCES AND BIOPROCESSING, vol. 4, no. 1, January 2017 (2017-01-01), pages 1 - 19, XP055550640 * |
| SHAHSAVAN, E. ET AL.: "Plant residues - A low cost, effective bioremediation treatment for petrogenic hydrocarbon-contaminated soil", SCIENCE OF THE TOTAL ENVIRONMENT, vol. 443, 2013, pages 766 - 774, XP055550646, Retrieved from the Internet <URL:https://doi.org/10.1016/j.scitotenv.2012.11.029> * |
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