WO2012008841A1 - Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture - Google Patents
Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture Download PDFInfo
- Publication number
- WO2012008841A1 WO2012008841A1 PCT/NO2010/000284 NO2010000284W WO2012008841A1 WO 2012008841 A1 WO2012008841 A1 WO 2012008841A1 NO 2010000284 W NO2010000284 W NO 2010000284W WO 2012008841 A1 WO2012008841 A1 WO 2012008841A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- substrate
- environment
- natural
- extracts
- bioremediation
- Prior art date
Links
- 239000000758 substrate Substances 0.000 title claims abstract description 18
- 239000000203 mixture Substances 0.000 title claims abstract description 12
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 10
- 239000000284 extract Substances 0.000 title claims abstract description 10
- 235000013311 vegetables Nutrition 0.000 title claims abstract description 10
- 239000000126 substance Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 6
- 108090000790 Enzymes Proteins 0.000 claims abstract description 6
- SYSQUGFVNFXIIT-UHFFFAOYSA-N n-[4-(1,3-benzoxazol-2-yl)phenyl]-4-nitrobenzenesulfonamide Chemical class C1=CC([N+](=O)[O-])=CC=C1S(=O)(=O)NC1=CC=C(C=2OC3=CC=CC=C3N=2)C=C1 SYSQUGFVNFXIIT-UHFFFAOYSA-N 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000002689 soil Substances 0.000 claims abstract description 3
- 238000003786 synthesis reaction Methods 0.000 claims abstract description 3
- 230000014621 translational initiation Effects 0.000 claims abstract description 3
- 102000004195 Isomerases Human genes 0.000 claims description 2
- 108090000769 Isomerases Proteins 0.000 claims description 2
- 102000003960 Ligases Human genes 0.000 claims description 2
- 108090000364 Ligases Proteins 0.000 claims description 2
- 102000008109 Mixed Function Oxygenases Human genes 0.000 claims description 2
- 108010074633 Mixed Function Oxygenases Proteins 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000004048 modification Effects 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims description 2
- 239000000356 contaminant Substances 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 239000002361 compost Substances 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000011358 absorbing material Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000016571 aggressive behavior Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/286—Treatment of water, waste water, or sewage by sorption using natural organic sorbents or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/68—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water
- C02F1/683—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water by addition of complex-forming compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/32—Hydrocarbons, e.g. oil
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/344—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of mineral oil
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/40—Valorisation of by-products of wastewater, sewage or sludge processing
Definitions
- the present request for an invention patent refers to an innovative technology developed from the use of enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where a synthesis reaction creating expressive conditions for bioremediation of carbon molecules takes place.
- the density is lower than water (even when associated to the oils) and chemically apolar (suffering total rejection by the aquatic environment) granting permanent floatability, impeding the reintroduction of the polluting element into the aquatic environment, and when on land, it impedes the mechanical action of the oils in the gas exchange processes between free 0 2 and the epicyclic environment when the polluting element is still in a contaminant stage.
- the production technology of the present invention has three distinct phases, which are:
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The present development brings a technical innovation for manipulating natural-origin enzymatic elements developed by using enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where there is a synthesis reaction creating expressive conditions for bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces. It is a process of using enzymes for bioremediation in a substrate, which due to its basic substances' characteristics, presents an inexpressive toxicity, high floatability degree and the ability to absorb contaminant in a ratio weight/weight in the range of 2.000% to 2.500%, and in the ratio volume/volume in the range of 100% to 200%. In the present process we present a technological innovation for manipulation of natural-origin enzymatic elements, using them in a specific way and for a specific purpose, which due to these characteristics, when in contact with liquid carbon substances provide a considerable reduction of the biodegradability time and the transformation of the bioremediator/polluent mix when collected in fully organic compost.
Description
"NATURAL -ORIGIN ENZYMATIC BIOREMEDIATION WITH WIDE
APPLICABILITY, THE SUBSTRATE BEING AN ENVIRONMENT
CONSISTING OF EXTRACTS FROM OLEAGINOUS VEGETABLE SOURCES ADDED TO AN ISOMER MIXTURE".
SPECIFICATION
The present request for an invention patent refers to an innovative technology developed from the use of enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where a synthesis reaction creating expressive conditions for bioremediation of carbon molecules takes place.
The necessity of the present development becomes evident when we face the reality of a permanent environmental threat: pollution by liquid substances containing carbon molecules, occasioned by human failure or break-down of systems or equipment.
When there is an accident, the methodology presently applied for the absorption of liquid carbon substances from the environment is using absorbing materials. However, due to its non-degradability, these materials have the inconvenience of substantially increasing the residue volume, sometimes causing worse harm to the environment. In some cases (like in the water), due to higher density and/or affinity with the aquatic environment, it makes the oil sink. In other cases, the lixiviation processes (the oil initially absorbed comes loose), make the pollutant return to the environment, and on land, it impedes the mechanic action of the oils in the gas exchange processes between free O2 and the epicyclic substrate.
The utilization of natural-origin polymeric enzymes as substrate in an environment consisting of extracts of oleaginous vegetable sources, added to a mixture of isomer, has considerable advantages in relation to the above
mentioned products for the removal of oily substances, because they are able to quickly transform the substances into a totally organic compound by action of the mentioned enzymes in this specific substrate (which, due to its qualities, foment an electrovalense action with the carbon molecules, adding it to the environment, which immediately puts an end to the aggression against the ecosystem). The density is lower than water (even when associated to the oils) and chemically apolar (suffering total rejection by the aquatic environment) granting permanent floatability, impeding the reintroduction of the polluting element into the aquatic environment, and when on land, it impedes the mechanical action of the oils in the gas exchange processes between free 02 and the epicyclic environment when the polluting element is still in a contaminant stage.
The production technology of the present invention has three distinct phases, which are:
1) - Mixing process of substrate - This phase proceeds from the mixture of extract of oleaginous vegetable sources with a mix of isomers forcing a binding and expansion reaction and the appearance of a polymeric substrate;
2) - Addition of predefined proportions of natural-origin polymeric biomolecules - Specific doses of: Oxdoreductases 12,5g, Hydroxylases 10g, Ligases 8,5g e Isomerases 10g on a culture base per m3;
3) Final processing - Structural physical modification of the product resulting from the enzymatic addition to the polymeric substrate.
The object of the present request for the Invention Patent, called "NATURAL -ORIGIN ENZYMATIC BIOREMEDIATION WITH WIDE APPLICABILITY, THE SUBSTRATE BEING AN ENVIRONMENT CONSISTING OF
EXTRACTS FROM OLEAGINOUS VEGETABLE SOURCES ADDED TO AN ISOMER MIXTURE" is an innovative enzymatic technology
introduced in a substrate of oleaginous vegetable extracts added to a mixture of isomers for the bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces, with an efficiency that is superior to existing technologies and products. Because the substrate is granulated, thus causing the addition to its environment of liquid oily contaminating substances in a ratio of weight/weight in the range of 2.000% to 2.500% and in the ratio volume/volume in the range of 100% to 200%, which, due to the substances' basic characteristics has an inexpressive toxicity degree and a high floatability degree, we have in this process come up with a technologic innovation which is a methodology of
manipulating the enzymatic elements from natural origin, using them in a specific way and for a specific purpose.
Claims
The present request for an invention patent refers to a technical innovation for manipulating natural-origin enzymatic elements developed by using enzymes for biopolymerization of a specific environment with digester/biodegrades characteristics, using as substrate an environment consisting of extracts of oleaginous vegetable sources added to a mixture of isomers, where there is a synthesis reaction creating expressive conditions for bioremediation of liquid carbon substances in the environment, in water, soil or non-porous surfaces.
Claim 1 , consists of a process for mixing the substrate where the extracts of oleaginous vegetable sources are blended into a mixture of isomers, causing a reaction of binding and expansion and the emergence of a polymeric substrate;
Claim 2, consists of addition in predefined proportions of natural origin polymeric biomolecules in specific doses of: Oxdoreductases 125g, Hydroxylases 100g, Ligases 80, 5g e Isomerases 100g on a culture base per m3;
Claim 3, consists of the final processing of the physical structural modification of the product which resulted from the enzymatic addition to the polymeric substrate.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/NO2010/000284 WO2012008841A1 (en) | 2010-07-14 | 2010-07-14 | Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture |
| EP10745684.0A EP2593406A1 (en) | 2010-07-14 | 2010-07-14 | Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture |
| BR112013000948-9A BR112013000948B1 (en) | 2010-07-14 | 2010-07-14 | method of enzymatic bioremediation of natural origin, product and its use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/NO2010/000284 WO2012008841A1 (en) | 2010-07-14 | 2010-07-14 | Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2012008841A1 true WO2012008841A1 (en) | 2012-01-19 |
Family
ID=43778166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/NO2010/000284 WO2012008841A1 (en) | 2010-07-14 | 2010-07-14 | Natural -origin enzymatic bioremediation with wide applicability, the substrate being an environment consisting of extracts from oleaginous vegetable sources added to an isomer mixture |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP2593406A1 (en) |
| BR (1) | BR112013000948B1 (en) |
| WO (1) | WO2012008841A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018170561A1 (en) * | 2017-03-22 | 2018-09-27 | Kolfinn Roger Walvag | Biorremediate process with bacterial, fungic and enzymatic having substrate a midst constituted by cellulose material |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5656490A (en) * | 1993-07-23 | 1997-08-12 | Wyatt; Caryl Heintz | Enzyme compositions and methods for biodegradation separation of natural fibers and adsorbed petroleum products |
| US5821113A (en) * | 1993-05-25 | 1998-10-13 | Envorflow Inc. | Method of reducing contamination and composition for use in the method |
| EP0979802A1 (en) * | 1997-04-16 | 2000-02-16 | Yugenkaisha FAMS | Process for recovering oils |
| WO2009043130A1 (en) * | 2007-10-03 | 2009-04-09 | Falnes Elingsen, Hans | Improved system and manufacturing process of bio- repair of substances in the liquid state which contain carbon molecules in its structure |
-
2010
- 2010-07-14 WO PCT/NO2010/000284 patent/WO2012008841A1/en active Application Filing
- 2010-07-14 BR BR112013000948-9A patent/BR112013000948B1/en not_active IP Right Cessation
- 2010-07-14 EP EP10745684.0A patent/EP2593406A1/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5821113A (en) * | 1993-05-25 | 1998-10-13 | Envorflow Inc. | Method of reducing contamination and composition for use in the method |
| US5656490A (en) * | 1993-07-23 | 1997-08-12 | Wyatt; Caryl Heintz | Enzyme compositions and methods for biodegradation separation of natural fibers and adsorbed petroleum products |
| EP0979802A1 (en) * | 1997-04-16 | 2000-02-16 | Yugenkaisha FAMS | Process for recovering oils |
| WO2009043130A1 (en) * | 2007-10-03 | 2009-04-09 | Falnes Elingsen, Hans | Improved system and manufacturing process of bio- repair of substances in the liquid state which contain carbon molecules in its structure |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018170561A1 (en) * | 2017-03-22 | 2018-09-27 | Kolfinn Roger Walvag | Biorremediate process with bacterial, fungic and enzymatic having substrate a midst constituted by cellulose material |
Also Published As
| Publication number | Publication date |
|---|---|
| BR112013000948B1 (en) | 2021-05-25 |
| BR112013000948A2 (en) | 2020-06-09 |
| EP2593406A1 (en) | 2013-05-22 |
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