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WO2018168582A1 - Pesticide, et procédé de fabrication de celui-ci - Google Patents

Pesticide, et procédé de fabrication de celui-ci Download PDF

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Publication number
WO2018168582A1
WO2018168582A1 PCT/JP2018/008631 JP2018008631W WO2018168582A1 WO 2018168582 A1 WO2018168582 A1 WO 2018168582A1 JP 2018008631 W JP2018008631 W JP 2018008631W WO 2018168582 A1 WO2018168582 A1 WO 2018168582A1
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Prior art keywords
fatty acid
insecticide
acid
proteobacteria
producing
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PCT/JP2018/008631
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English (en)
Japanese (ja)
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大野　勝也
久美子 高田
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イビデン株式会社
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Priority to JP2019505911A priority Critical patent/JPWO2018168582A1/ja
Publication of WO2018168582A1 publication Critical patent/WO2018168582A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria

Definitions

  • the present invention relates to an insecticide and a method for producing the insecticide.
  • drugs such as pyrethrin, which is an active ingredient of pesticide chrysanthemums, and synthetic pyrethroids such as allethrin derived from pyrethrin derivatives have been used. While pyrethroids have low toxicity to mammals and birds, they act strongly on insect nerve cells to produce neurotoxins and kill insects. In recent years, neonicotinoid insecticides that kill insects by binding to the insect neurotransmitter acetylcholine receptor have been widely used as insecticides.
  • insects are directly killed using components that are generally toxic to such insects
  • beneficial insects may be affected over a long period of time with insecticides that have a long residual effect, such as neonicotinoids, due to their ability to penetrate into plants.
  • insecticides that have a long residual effect, such as neonicotinoids, due to their ability to penetrate into plants.
  • insecticide-resistant insects due to excessive application of agricultural chemicals is also a problem. From the viewpoint of environmental impact such as soil contamination, reduction of the amount of chemically synthesized pesticides is desired.
  • Patent Document 1 describes a microbial biosurfactant.
  • Patent Document 1 discloses a method for controlling pests using a fermentation broth filtrate containing rhamnolipid as a biosurfactant, which can be used as an insecticide for controlling house flies and nematodes. Are listed. However, the drug described in Patent Document 1 has a problem that even large-sized pests such as moth larvae cannot be killed.
  • the present invention has been made in view of the above problems, and an object of the present invention is to provide an insecticide having a low soil contamination and toxicity and having an excellent insecticidal effect, and a method for producing the same.
  • the present invention relates to an insecticide containing a fatty acid metabolite obtained by metabolizing a fatty acid having 4 to 30 carbon atoms to proteobacteria under a dissolved oxygen concentration environment of 0.1 to 8 mg / l (liter).
  • fatty acid is a fatty acid that is liquid at 20 ° C.
  • An insecticide wherein the metabolism is metabolism in the presence of at least one mineral selected from Mg, P, Na and K is preferred.
  • An insecticide wherein the proteobacteria are pre-cultured proteobacteria is preferred.
  • the pre-cultured proteobacterium is a proteobacterium pre-cultured to 1 ⁇ 10 8 to 9 ⁇ 10 10 cells / ml (milliliter).
  • the insecticide is an insecticide containing a biosurfactant.
  • Insecticides in which the metabolism is metabolism under conditions of 20 to 30 ° C. are preferred.
  • the present invention also relates to a method for producing an insecticide containing a fatty acid metabolite comprising a fatty acid metabolism step of metabolizing a fatty acid having 4 to 30 carbon atoms to proteobacteria under a dissolved oxygen concentration environment of 0.1 to 8 mg / l. .
  • a method for producing an insecticide wherein the fatty acid is a fatty acid which is liquid at 20 ° C. is preferred.
  • a method for producing an insecticide wherein the fatty acid metabolism step is carried out in the presence of at least one mineral selected from Mg, P, Na and K is preferred.
  • a method for producing an insecticide wherein the proteobacteria are pre-cultured proteobacteria is preferred.
  • Proteobacteria said preincubated
  • the method for producing a pesticide is preferably proteobacteria, which is pre-cultured in the number 1 ⁇ 10 8 ⁇ 9 ⁇ 10 10 cells / ml bacteria.
  • a method for producing an insecticide which is a method for producing an insecticide containing a biosurfactant, is preferred.
  • a method for producing an insecticide wherein the fatty acid metabolism step is carried out at 20-30 ° C. is preferred.
  • the insecticide of the present invention has low soil contamination and toxicity and is excellent in insecticidal effect. Furthermore, according to the method for producing an insecticide of the present invention, an insecticide having low soil contamination and toxicity and excellent insecticidal effect can be produced.
  • the insecticide of the present invention comprises a fatty acid metabolite obtained by metabolizing a fatty acid having 4 to 30 carbon atoms to proteobacteria under a dissolved oxygen concentration environment of 0.1 to 8 mg / l. .
  • insects By bringing fatty acid metabolites into contact with pests, pests, particularly micro-pests, can be killed by airway blockade.
  • the insecticide of the present invention is considered to be effective for micro-insects that are early in generation change so that drug resistance to insecticides can be easily obtained, and for insects that have developed drug resistance against conventional insecticides.
  • fatty acid metabolites are much less toxic than chemically synthesized pesticides.
  • fatty acid metabolites are a group of genes involved in the production of infection-specific proteins (pathogenesis-related (PR) protein), salicylic acid or It is considered that the gene group involved in the production of enzymes that produce jasmonic acid is activated to induce systemic acquired resistance in plants. This is presumably because the fatty acid metabolite contains a substance that activates the salicylic acid pathway or jasmonic acid pathway related to resistance induction or a precursor of this substance.
  • PR pathogenesis-related
  • Metabolism in the present invention means that a fatty acid having 4 to 30 carbon atoms is decomposed by an enzyme or the like secreted by proteobacteria under an environment of dissolved oxygen concentration of 0.1 to 8 mg / l.
  • a method of culturing proteobacteria in a medium containing fatty acid under a dissolved oxygen concentration environment of 0.1 to 8 mg / l can be mentioned.
  • Proteobacteria have a gene that produces lipoxygenase (LOX), an enzyme involved in fatty acid metabolism, and can produce fatty acid metabolites.
  • LOX lipoxygenase
  • the carbon number of the fatty acid used in the present invention is 4-30, and preferably 10-20.
  • the melting point / boiling point is low, and therefore, the volatility tends to increase at the temperature at the time of culturing and hardly remain in the medium.
  • the number of carbons exceeds 30, the melting point / boiling point becomes high, so that it becomes a solid at the temperature at the time of culturing and tends to be separated without being mixed with the medium.
  • the melting point may not depend only on the number of carbons depending on the number of hydrogen bonds.
  • the fatty acid used in the present invention is preferably a liquid at 20 to 30 ° C. and more preferably a liquid at 20 ° C. from the viewpoint of metabolic efficiency and suppression of solidification in the medium.
  • the fatty acid of the present invention can be a saturated fatty acid, an unsaturated fatty acid, or a mixture containing both.
  • a free fatty acid (monocarboxylic acid) is preferable because it is excellent in a decomposition rate.
  • Free fatty acids having 4 to 30 carbon atoms include butyric acid (butyric acid), valeric acid (valeric acid), caproic acid, enanthic acid (heptylic acid), caprylic acid, pelargonic acid, capric acid, lauric acid, myristic acid, pentadecyl Acid, palmitic acid, palmitoleic acid, margaric acid, stearic acid, oleic acid, vaccenic acid, linoleic acid, ⁇ -linolenic acid, ⁇ -linolenic acid, eleostearic acid, arachidic acid, mead acid, arachidonic acid, behenic acid, Examples include lignoceric acid, nervonic acid, serotic acid, montanic acid, and melicic acid.
  • capric acid having 10 to 20 carbon atoms, lauric acid, myristic acid, pentadecylic acid, palmitic acid, palmitoleic acid, margaric acid, stearin Acid, oleic acid, vaccenic acid, linoleic acid, ⁇ -linolenic acid, ⁇ Linolenic acid, eleostearic acid, arachidic acid, mead acid, arachidonic acid are preferred, oleic acid having 18 carbon atoms, linoleic acid, alpha-linolenic acid, .gamma.-linolenic acid are more preferred.
  • the content of fatty acid is preferably 120 g / l or less, more preferably 100 g / l or less, and even more preferably 60 g / l or less. If it exceeds 120 g / l, emulsification with water in the medium becomes difficult, which may deteriorate metabolic efficiency and inhibit the growth of proteobacteria.
  • the lower limit of the fatty acid content is not particularly limited, but is preferably 1.0 g / l or more.
  • the medium containing fatty acid contains other mineral components.
  • a mineral component it is not specifically limited, The mineral component normally used for microorganism culture can be mentioned.
  • the component which has magnesium (Mg), phosphorus (P), sodium (Na), or potassium (K) is mentioned. These components can be used alone or in combination. Preferably two of these components may be used, more preferably three or more.
  • the content of the mineral component in the medium is not particularly limited, and can be the amount used in the conventional aerobic bacterium culture method, but since salt damage may occur during application to plants, preferably It can be used at 15 g / l or less, more preferably at 10 g / l or less.
  • proteobacteria used in the present invention is not particularly limited as long as the effects of the present invention are not impaired. From the viewpoint of fatty acid metabolism efficiency and growth efficiency, proteobacteria having a temperature suitable for growth (optimum temperature) of 10 to 40 ° C. are preferred, and proteobacteria having 20 to 30 ° C. are more preferred.
  • the proteobacteria are preferably pre-cultured proteobacteria because they are excellent in fatty acid metabolic efficiency, and the number of bacteria is preferably pre-cultured to 1 ⁇ 10 8 to 9 ⁇ 10 10 cells / ml. preferable.
  • metabolism is performed in a dissolved oxygen concentration environment of 0.1 to 8 mg / l.
  • the dissolved oxygen concentration is less than 0.1 mg / l, the activity of proteobacteria tends to decrease and the metabolic efficiency of fatty acids tends to be extremely low.
  • the dissolved oxygen concentration exceeds 8 mg / l, in parallel with the metabolic process by proteobacteria, decomposition of the fatty acid as a substrate by oxygen in the medium proceeds, resulting in a decrease in metabolic efficiency, and consequently an active ingredient. There is a risk that the production amount of the metabolite is reduced.
  • the dissolved oxygen concentration is 0.1 to 5 mg / l, more preferably 0.1 to 4 mg / l.
  • the dissolved oxygen concentration is a value measured by a diaphragm galvanic electrode method or a diaphragm polarographic method on a PO electrode with a dissolved oxygen meter manufactured by HORIBA, Ltd.
  • the temperature in metabolism can be appropriately adjusted according to the proteobacteria used, and is preferably 20 to 30 ° C. from the viewpoint of fatty acid metabolic efficiency.
  • the insecticide may contain a biosurfactant in addition to the fatty acid metabolite.
  • Fatty acid metabolites are easily dispersed in water, which is considered preferable from the viewpoint of handling of insecticides.
  • the biosurfactant according to the present invention means a surfactant-like substance that is produced by a microorganism to take up a highly hydrophobic substance and secreted outside the cell.
  • the biosurfactant secreted by proteobacteria facilitates the dispersion of fatty acid metabolites in water, so that spray treatment of insecticides containing fatty acid metabolites can be performed efficiently and easily.
  • biosurfactants not only biosurfactants produced by the proteobacteria of the present invention at the time of fatty acid degradation, but also biosurfactants produced by other microorganisms may be used, that is, the insecticides of the present invention. Biosurfactants produced by other microorganisms may be further added. Compared to artificially synthesized surfactants, biosurfactants are less toxic to living organisms and are also more biodegradable, which may result in more environmentally friendly insecticides. In addition, in order to promote fatty acid degradation by proteobacteria, biosurfactants produced by other microorganisms may be added during fatty acid degradation by proteobacteria. Fatty acid uptake by proteobacteria may be promoted.
  • the insecticide of the present invention is an air-sealed insecticide with low soil contamination and toxicity but excellent insecticidal effect. Furthermore, when the insecticide of the present invention is applied to a plant, it can induce systemic acquired resistance to the plant to increase the expression level of the resistance gene, thereby allowing the plant to produce an insecticidal component against the pest. Moreover, since the insecticide of the present invention can express both upstream and downstream genes in both the salicylic acid pathway and the jasmonic acid pathway, it is considered that resistance induction persists and a sustained insecticidal effect is obtained. .
  • the insecticide of the present invention can be used to kill micro pests.
  • the micro pests include, but are not limited to, pests such as aphids such as cotton aphids, spider mites, scarabs, crabs, and wasps and / or their larvae.
  • the method of applying the insecticide of the present invention is not particularly limited. For example, it can be applied to pests, pested plants and / or soil. Moreover, the plant to be applied is not limited, and it can be used favorably for plants in general. Examples thereof include dicotyledonous plants such as eggplant, cucurbitaceae, and roseceae, and monocotyledonous plants such as gramineous.
  • the method for producing an insecticide containing a fatty acid metabolite of the present invention includes a fatty acid metabolism step of metabolizing a fatty acid having 4 to 30 carbon atoms to proteobacteria in a dissolved oxygen concentration environment of 0.1 to 8 mg / l. It is characterized by.
  • the fatty acid metabolism step is a step in which a fatty acid having 4 to 30 carbon atoms is decomposed by an enzyme or the like that is secreted or secreted by proteobacteria under a dissolved oxygen concentration environment of 0.1 to 8 mg / l.
  • an enzyme or the like that is secreted or secreted by proteobacteria under a dissolved oxygen concentration environment of 0.1 to 8 mg / l.
  • the dissolved oxygen concentration in the fatty acid metabolism process is 0.1 to 8 mg / l.
  • the dissolved oxygen concentration is less than 0.1 mg / l, the activity of proteobacteria tends to decrease and the metabolic efficiency of fatty acids tends to be extremely low.
  • the dissolved oxygen concentration exceeds 8 mg / l, in parallel with the metabolic process by proteobacteria, decomposition of the fatty acid as a substrate by oxygen in the medium proceeds, resulting in a decrease in metabolic efficiency, and consequently an active ingredient. There is a risk that the production amount of the metabolite is reduced.
  • the dissolved oxygen concentration is 0.1 to 5 mg / l, more preferably 0.1 to 4 mg / l.
  • the dissolved oxygen concentration is a value measured by a diaphragm galvanic electrode method or a diaphragm polarographic method on a PO electrode with a dissolved oxygen meter manufactured by HORIBA, Ltd.
  • the dissolved oxygen concentration can be adjusted by the culture vessel, the number of shakes, the amount of aeration, etc.
  • the culture conditions in the fatty acid metabolism step can be the same as the conventional conditions for culturing aerobic bacteria except that the dissolved oxygen concentration is within a predetermined range. Examples thereof include a method of culturing for 3 to 7 days by shaking with a flask and aeration culture with a spinner flask or a jar fermenter.
  • the number of days of culture is preferably the number of days during which the fatty acid is sufficiently emulsified and decomposed, but the number of days of culture varies depending on the agitation and the amount of bacteria.
  • the fatty acid decomposition state is determined by measuring the absorbance at a wavelength of 230 nm, thin layer chromatography (TLC), high performance liquid chromatography (HPLC), gas chromatography mass spectrometry (GC / MS), liquid chromatography. It is preferable to confirm by graph mass spectrometry (LC / MS) or the like.
  • the temperature in the fatty acid metabolism step can be appropriately adjusted according to the proteobacteria used, and it is preferable to carry out the treatment at 20 to 30 ° C. from the viewpoint of fatty acid metabolic efficiency.
  • fatty acid and proteobacteria in the fatty acid metabolism step those described above in the description of the insecticide of the present invention can be used.
  • the pre-culturing step for proteobacteria is not particularly limited, and a normal method for culturing aerobic bacteria can be used. It is preferable that only the cells are collected from the preculture solution by centrifugation or the like and used in the fatty acid metabolism step.
  • the insecticide obtained by the production method of the present invention can contain a biosurfactant in addition to a fatty acid metabolite.
  • the biosurfactant according to the present invention means a surfactant-like substance that is produced by a microorganism to take up a highly hydrophobic substance and secreted outside the cell.
  • the biosurfactant secreted by proteobacteria also facilitates the dispersion of fatty acid metabolites in water. It is thought that the handling property of the insecticide is improved.
  • the insecticide of the present invention can be obtained as a culture solution that is a mixture with a culture medium, a proteobacterial exocrine product containing a biosurfactant, microbial cells, and the like.
  • the culture solution may be used as it is as the insecticide of the present invention, or the supernatant obtained by removing the cells from the culture solution by centrifugation or the like may be used as the insecticide.
  • the culture solution can be used as it is, but when applied to plants with pests, if the solution is used as it is, the treated part of the plant will evaporate and concentrate due to osmotic pressure at high temperatures. It is desirable to dilute the stock solution for use.
  • the dilution factor is not particularly limited as long as the effect of the present invention is exhibited, but is preferably 10 to 1000 times diluted, more preferably 10 to 100 times diluted.
  • Preparation of insecticide for test ⁇ Pre-culture process> 20 g of peptone (Difco protein enzyme hydrolyzate), 1.5 g of magnesium sulfate heptahydrate and 1.5 g of dipotassium hydrogen phosphate in 1 l (liter) of water in a glass Erlenmeyer flask, or 1 l (liter) ) 10 g of peptone (Difco protein enzyme hydrolyzate), 5 g of yeast extract and 10 g of sodium chloride are dissolved in autoclaved water at 121 ° C. for 20 minutes, cooled to room temperature, Inoculated. The mouth of the culture vessel was sealed with a silicon stopper.
  • the inoculated container was cultured for 24 hours under the conditions of 25 ⁇ 5 ° C. and 120 rpm using a bioshaker (BR-23UM manufactured by Taitec Corporation).
  • the number of bacteria in the culture was 5 ⁇ 10 8 cells / ml.
  • the cells were collected from the culture solution by centrifuging the culture solution under conditions of 15,000 ⁇ G and 20 ° C.
  • ⁇ Fatty acid metabolism process In 1 l (liter) of sterilized water in a glass Erlenmeyer flask, 12 g of linoleic acid (primary linoleic acid manufactured by Wako Pure Chemical Industries, Ltd.), 1.5 g of magnesium sulfate heptahydrate and / or dipotassium hydrogen phosphate 1 .5 g and the total amount of the cells obtained from the pre-culture step were added. This was cultured for 4 days under the conditions of 20 ° C., 120 rpm, and dissolved oxygen concentration of 4 mg / l using a bioshaker (BR-23UM manufactured by Taitec Corporation).
  • a bioshaker BR-23UM manufactured by Taitec Corporation
  • the degradation of linoleic acid involves measuring the concentration of oxidized lipid, which is one of linoleic acid intermediate products, in the culture solution using a spectrophotometer BioSpec-mini manufactured by Shimadzu Corporation at a wavelength of 230 nm. Confirmed by. The following evaluation was performed after culture
  • Example 1 A cucumber true leaf having a maximum length of 10 cm was cut into 2 cm squares, and 20 adult cotton aphids were fixed on the back of the leaf.
  • the test insecticide stock solution (Example 1) and its 10-fold and 100-fold dilutions (Examples 2 and 3) were sprayed onto the worm bodies at a spray amount of 190 ⁇ l / cm 2 .
  • Leaves and insects were placed in a petri dish, sprayed with the insecticide of the present invention, and the number of live and dead insects was measured 24 hours later. As a control, the insect body was sprayed with water, and the number of live and dead insects of the test insects was measured in the same manner.
  • Comparative examples 1-6 instead of the test pesticide, the existing air-sealed insecticide containing sodium oleate as an active ingredient (Comparative Example 1) and the air-sealed insecticide containing hydroxypropyl starch as an active ingredient (Comparative Example) 2) Insecticide containing pyrethrin as an active ingredient (Comparative Example 3), Insecticide containing acetamiprid as an active ingredient (Comparative Example 4), 1% solution of Surfactin sodium as a typical biosurfactant (Comparative Example 5) And the corrected mortality was determined in the same manner as in Examples 1 to 3 except that 1% SDS (Comparative Example 6) was used. The results are shown in FIG.
  • the insecticide of the present invention has an excellent insecticidal effect equivalent to that of conventional air-sealed insecticides, pyrethroids and acetamiprid insecticides.
  • Example 4 The moth larvae collected from roses cultivated outdoors were raised at about 25 ° C. for 48 hours, and then divided into three groups (less than 1.0 cm, 1.0 to 2.0 cm, and more than 2.0 cm) according to body length. . Rose leaves were placed in the petri dish, and one moth larvae from any one of the three groups was placed thereon. For each group, 4 (less than 1.0 cm long), 5 (length 1.0-2.0 cm), 5 (greater than 2.0 cm) moth larvae were used. A diluted solution obtained by diluting the test insecticide 30 times with water was sprayed on the worm body with a spray amount of 1.1 ml. The petri dish lid was closed and allowed to stand in the dark. After 24 hours, the moth larvae were observed for life and death, and the death rate was produced. As a control, moth larvae sprayed with water were similarly tested. The results are shown in Table 1.
  • the insecticide of the present invention has an excellent insecticidal effect, particularly a high insecticidal effect against micro pests.
  • insecticide of the present invention and the insecticide produced by the production method of the present invention are insecticides having low soil contamination and toxicity and excellent insecticidal effect.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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Abstract

L'invention fournit un pesticide faible en termes de pollution des sols et de toxicité, mais présentant un excellent effet pesticide, et fournit également un procédé de fabrication de ce pesticide. Plus précisément, l'invention concerne un pesticide qui contient un métabolite d'acide gras obtenu par métabolisme d'un acide gras de 4 à 30 atomes de carbone en protéobactérie sous un environnement de concentration en oxygène dissous comprise entre ０,１ et ８ｍｇ／ｌ. L'invention concerne également un procédé de fabrication de pesticide contenant un métabolite d'acide gras, qui inclut une étape de métabolisme d'acide gras au cours de laquelle un acide gras de 4 à 30 atomes de carbone est métabolisé en protéobactérie sous un environnement de concentration en oxygène dissous comprise entre ０,１ et ８ｍｇ／ｌ.
PCT/JP2018/008631 2017-03-14 2018-03-06 Pesticide, et procédé de fabrication de celui-ci WO2018168582A1 (fr)

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JP2019505911A JPWO2018168582A1 (ja) 2017-03-14 2018-03-06 殺虫剤および殺虫剤の製造方法

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JP2017-048721 2017-03-14
JP2017048721 2017-03-14

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11514365A (ja) * 1995-10-20 1999-12-07 マイコーゲン コーポレーション 線虫を制御するための材料および方法
JP2007137888A (ja) * 1997-12-08 2007-06-07 Koa Corp 植物用薬剤
JP2013521227A (ja) * 2010-02-25 2013-06-10 マロン バイオ イノベイションズ インコーポレイテッド バークホルデリア（Ｂｕｒｋｈｏｌｄｅｒｉａ）属の単離菌株およびそれに由来する殺虫性代謝物
JP2013543846A (ja) * 2010-10-25 2013-12-09 マロン バイオ イノベイションズ インコーポレイテッド クロモバクテリウムの生物活性組成物および代謝産物
WO2018047918A1 (fr) * 2016-09-08 2018-03-15 イビデン株式会社 Activateur pour végétaux, et procédé de fabrication de celui-ci

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11514365A (ja) * 1995-10-20 1999-12-07 マイコーゲン コーポレーション 線虫を制御するための材料および方法
JP2007137888A (ja) * 1997-12-08 2007-06-07 Koa Corp 植物用薬剤
JP2013521227A (ja) * 2010-02-25 2013-06-10 マロン バイオ イノベイションズ インコーポレイテッド バークホルデリア（Ｂｕｒｋｈｏｌｄｅｒｉａ）属の単離菌株およびそれに由来する殺虫性代謝物
JP2013543846A (ja) * 2010-10-25 2013-12-09 マロン バイオ イノベイションズ インコーポレイテッド クロモバクテリウムの生物活性組成物および代謝産物
WO2018047918A1 (fr) * 2016-09-08 2018-03-15 イビデン株式会社 Activateur pour végétaux, et procédé de fabrication de celui-ci

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OHNO, KATSUYA: "Approach of Plant Responses to Plant Disease Control by Bacterial Metabolites of Fatty Acid (BMFA) (1", ANNUAL MEETING OF THE JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 5 March 2017 (2017-03-05) *

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