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WO2018001679A1 - Dispositif de protection contre les contaminations pour un système de microdissection laser et système de microdissection laser - Google Patents

Dispositif de protection contre les contaminations pour un système de microdissection laser et système de microdissection laser Download PDF

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Publication number
WO2018001679A1
WO2018001679A1 PCT/EP2017/063572 EP2017063572W WO2018001679A1 WO 2018001679 A1 WO2018001679 A1 WO 2018001679A1 EP 2017063572 W EP2017063572 W EP 2017063572W WO 2018001679 A1 WO2018001679 A1 WO 2018001679A1
Authority
WO
WIPO (PCT)
Prior art keywords
contamination protection
laser
protection plate
contamination
laser microdissection
Prior art date
Application number
PCT/EP2017/063572
Other languages
German (de)
English (en)
Inventor
Dr. Falk SCHLAUDRAFF
Manfred Gilbert
Original Assignee
Leica Microsystems Cms Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leica Microsystems Cms Gmbh filed Critical Leica Microsystems Cms Gmbh
Publication of WO2018001679A1 publication Critical patent/WO2018001679A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/32—Micromanipulators structurally combined with microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving
    • G01N2001/2886—Laser cutting, e.g. tissue catapult

Definitions

  • Contamination protection device for a laser microdissection system and laser microdissection system
  • the present invention relates to a contamination protection device for a laser microdissection system, wherein the contamination protection device has a contamination protection plate with an opening. Furthermore, the invention relates to a laser microdissection system with such a contamination protection device, which is designed such that the contamination protection plate between a sample holder for receiving a sample to be dissected and a collecting holder for receiving a Lasermikrodissektat- collecting container can be arranged such that the opening of the contamination protection plate in the Opening of the collection container is located.
  • a dissectate can be isolated from a sample by means of an infrared or ultraviolet laser beam which falls under the influence of gravity into a suitable dissektate collection container. The dissecting can thereby also be cut out of the sample together with a membrane attached to the sample.
  • a thermoplastic membrane is heated by means of a corresponding laser beam. The membrane fuses with the desired area of the sample and can be removed by tearing in a subsequent step.
  • Another alternative is to attach the dissectate by means of the laser beam to a lid of a Dissektatauffang practicers.
  • upwardly transported dissectates may also be attached to the bottom of a dissectate collection container provided with an adhesive coating.
  • laser microdissection systems which have laser deflection or laser scanning devices which are set up to move the laser beam or its point of impact on the stationary sample to be dissected are more advantageous.
  • Such laser microdissection systems which are also to be used in the context of the present invention and offer special advantages there, are explained in detail below.
  • a particularly advantageous laser microscope system which has a laser deflection device with mutually adjustable glass wedges in the laser beam path is described, for example, in EP 1 276 586 B1.
  • each hole by a laser pulse or a depression is created in the sample.
  • a cutting line is created by a juxtaposition of such holes or depressions, optionally with overlap.
  • the laser microdissection can be used to obtain single cells or defined areas of tissue with diameters of typically less than 250 ⁇ , but usually only about 20 ⁇ or less, which are separated by a laser beam from the surrounding tissue and subsequently subjected to different diagnostic analysis methods, for example.
  • laser microdissection can be used to isolate specific (tumor) cells from a microscopic section and to examine them for specific metabolites or proteins.
  • integrated contamination protection devices exist in known laser microdissection systems.
  • the contamination protection device is laterally rigidly screwed to the microscope of the laser microdissection system and has a Kontaminationstikblech, which separates the sample holder and the collection holder of the laser microdissection system from each other.
  • the Kontaminationstikblech has an opening or a hole through which or cut the dissected material can enter the collection container.
  • a disadvantage of the known contamination protection devices is that the contamination protection plates are not adjustable after attachment of the contamination protection device.
  • the contamination guard may bend and sag under the influence of its own weight. Due to this change in shape, a much larger opening must always be left in the contamination protection plate to ensure the widest field of view (FOV) of the available objectives without shading and for the passage of the laser microdissectates than actually necessary.
  • FOV field of view
  • the distance between the sample holder and collector can be difficult to minimize or even changed in the existing facilities.
  • the present invention therefore has as its object to provide an improved contamination protection device for a laser microdissection system, which avoids in particular the disadvantages mentioned above.
  • the present invention provides a contamination protection device for a laser microdissection system and a laser microdissection system with such a contamination protection device according to the independent patent claims.
  • Advantageous embodiments are the subject of the respective subclaims and the following description.
  • the contamination protection device has at least one tensioning device for tensioning the contamination protection plate, and the contamination protection device is designed to arrange the contamination protection plate between the sample holder and a collection holder for the laser microdissection collection container of the laser microdissection system such that the opening of the contamination protection plate in the region of the opening of the collection container lies.
  • the tensioning device can tension the contamination protection plate in one or more extension directions of this plate so that bending or sagging of the plate is prevented.
  • a clamping device may be arranged on one side of the plate, wherein the plate on the other opposite side is rigidly connected to the contamination protection device or with a corresponding suspension for the plate.
  • the plate can be tensioned in a first direction of extension (x-direction). If necessary, the plate can be in one vertical direction (y-direction) are additionally tensioned by a second clamping device.
  • the invention further relates to a laser microdissection system comprising a sample holder for receiving a sample to be dissected and a collection holder for receiving a laser microdissection collection container which excises a laser microdissectate from the sample with a laser beam and traps it in the laser microdissectate collection container.
  • a contamination prevention device is provided, which comprises a contamination protection plate with an opening.
  • the contamination protection device has at least one tensioning device for tensioning the contamination protection plate, and the contamination protection device is designed to arrange the contamination protection plate between the sample holder and a collection holder for the laser microdissection collection container of the laser microdissection system such that the opening of the contamination protection plate in the region of the opening of the collection container lies.
  • the contamination protection device it is possible, for example, to cover unused collecting containers for the laser microdissection during the laser microdissection process and at the same time to produce a minimum distance between the sample to be dissected and the corresponding active collecting container. Due to the clamping device, the thickness of the contamination protection plate can be chosen lower than before. At the same time a sagging of the plate is avoided. The opening in the plate ensures the unimpeded transfer of microdissectates into the selected active collection container in the receiver. Since sagging of the contamination protection plate is avoided, the minimum necessary diameter of the opening in the plate can be selected. It corresponds to the widest field of view (FOV) of the lenses available in the laser microdissection system.
  • FOV widest field of view
  • the opening must be aligned in such a way that no shading by the contamination protection plate is visible when looking through all available lenses. Due to the tensioning device, the contamination protection plate can not push through, so that compared with the prior art, no greater distance to the collecting container must be selected to prevent wedging of collecting containers or the active collecting container with the opening edge of Kontaminationstikplatte. Consequently, it is always possible to maintain the minimum necessary distance between the collecting container and the sample, which is advantageous, in particular in the case of microdissect collection by gravity, in order to minimize the dropping distance and to make the protection against contamination even more effective.
  • the contamination protection device has at least one adjusting device for adjusting the contamination protection plate in at least one spatial direction.
  • the at least one adjusting device makes it possible to optimally adjust the contamination protection plate in at least one spatial direction.
  • the plate in one or both of the main directions of extension of the plate can be adjusted, whereby the position of the opening in the plate can be adjusted relative to the opening of the collecting container.
  • the position of the plate in the direction perpendicular to this plate spatial direction z-direction
  • the position of the plate between the collecting holder and the sample holder of the laser microdissection system can be optimally adjusted by an adjusting device.
  • the diameter of the opening in the contamination protection plate can be chosen as low as possible without running the risk of shading caused by the edge of the opening at a rigid attachment.
  • this smallest possible diameter is the one which ensures the largest possible field of view (FOV) in accordance with the available objectives.
  • the clamping device comprises a resilient element.
  • the spring force of the resilient element acts in at least one extension direction of the contamination protection plate such that the plate is tensioned at least in this direction in order to prevent sagging.
  • the Plate on two opposite sides of the plate suspended or clamped so that one side of the plate is firmly connected to the suspension, while the suspension of the plate is supported on the other side against a resilient element such as a spring, wherein the voltage of the Spring is set or adjusted so that sagging of the plate is prevented at any time.
  • the adjusting device comprises a screw adjustment which is set up such that the contamination protection plate is displaced in at least one spatial direction by an adjustment of the screw adjustment.
  • screw adjustments are known per se to the person skilled in the art.
  • Such a screw adjustment can for example be realized in that the contamination protection plate is suspended on two opposite sides, the suspensions are movably mounted on both sides and one of the two bearings contains a screw adjustment, via which the position of the plate can be changed by adjusting the screw can.
  • a similar adjustment by means of screw adjustment for the z-direction can be realized.
  • the contamination protection plate is a thin sheet or a thin plastic plate or a thin but stable foil.
  • the thickness of the plate can be between 0, 1 and 2 mm. Due to the voltage of the contamination protection plate whose thickness can be selected smaller than usual in the prior art. As a result, in turn, the distance of the sample or of the sample holder to the collecting holder or to the opening of the collecting container of the laser microdissection system can be selected to be lower than in the prior art. As a result, possible contaminations are even better avoided and at the same time the smallest possible (drop) distance between the sample and collecting container.
  • the contamination protection device is advantageously designed such that the contamination protection plate is to be arranged between a sample holder and a collection holder for a laser microdissection collection container of the laser microdissection system such that the opening of the contamination protection plate lies in the region of the opening of the (active) collection container.
  • the opening must be adjusted in such a way that no visible field shading is generated for all available lenses.
  • the invention further relates to a laser microdissection system with a contamination protection device according to the invention. Reference is made to the above explanations.
  • a laser microdissection system is based, in particular, on a laser microdissection device known per se with a microscope comprising a laser light source for generating a laser beam, a deflection device for deflecting the laser beam, an incident light device for focusing the laser beam through a microscope objective onto a sample, a microscope stage for receiving a sample holder for holding the sample to be dissected and a collecting holder for receiving a laser microdissection collecting container.
  • the laser beam from a laser light source is coupled into the observation beam path of the microscope in such a laser microdissection system.
  • the laser beam is transmitted through the microscope objective, which is also used for viewing the sample, to these foils. kussiert.
  • the laser microdissection system used in the context of the present invention is used with samples which have already been prepared for microscopy. These may be, for example, tissue thin sections which have been separated out of a larger tissue block by means of a microtome. Such a tissue block may be, for example, a fixed organ or a biopsy of a corresponding organ.
  • the laser microdissection system according to the invention therefore does not serve to obtain samples but to process them and to isolate certain areas thereof.
  • the present invention may also be used with samples that are not obtained by means of a microtome, e.g. with smears, macerates, etc. As mentioned, however, the invention is also suitable for processing thicker samples which have not been prepared by means of a microtome.
  • Microtomes are used exclusively in the preparation of microscopic samples. Microtomes can also have lasers for this purpose. The sections obtained by means of a microtome are applied to a microscope slide as mentioned above, optionally attached there, stained, etc. Only then are these available for use in the laser microdissection system.
  • a microtome differs fundamentally in its operation from a laser microdissection system in that it produces cuts with as homogeneous a cutting thickness as possible. Microtomes are therefore designed to produce a large number of identical sections with parallel cut surfaces, whereas laser microdissection systems are set up for separating dissectates according to sample-dependent criteria, for example according to visual morphological criteria.
  • the laser microdissection system is used in particular for separating out sample parts , which are subsequently taken up in a suspending fluid.
  • the person skilled in the art would therefore find technical solutions used in microtomes. Because of the completely different objectives, they are not transferred to such laser microdissection systems.
  • Figure 1 shows a laser microdissection system, which is preferably the starting point of the present invention, in a schematic representation.
  • FIG. 2 schematically shows parts of the laser microdissection system shown in FIG. 1 together with an embodiment of the contamination protection device according to the invention.
  • a laser microdissection system or an abovementioned laser microdissection device which can be used for carrying out the invention, is shown schematically and designated by 100 as a whole.
  • the laser microdissection system 100 corresponds in substantial parts to that disclosed in EP 1 276 586 B1, to which reference is expressly made.
  • the laser microdissection system 100 comprises a microscope 10.
  • an illumination device 12 which is only partially illustrated here, can be provided.
  • This may, for example, comprise a light source (not shown) and suitable means for influencing the illumination light provided by the light source, for example filters and / or diaphragms.
  • a condenser unit 90 can be provided for transmitted light illumination and for setting suitable contrast or observation methods.
  • a user input and / or user information unit 13 may be arranged, which may be formed, for example, as a touch screen, and via which the user can enter and / or read, for example, viewing and / or processing parameters.
  • a drive knob 14 is provided. This serves to operate a coarse and a fine drive for adjusting a height of a microscope stage 30.
  • a sample 51 for example a tissue sample attached in a corresponding slide or a sample holder 52, can thereby be brought into an object plane of an objective 41.
  • the objective 41 is fastened next to other objectives 42 in a nosepiece 40.
  • a protective cover 15 may be provided.
  • Observation light emanating from the sample 51 runs along an observation beam path a.
  • a preferably variable portion of the observation light for example by 60 °, can be coupled out and presented to a user by means of an eyepiece pair 62.
  • Another portion of the observation light can be coupled into a digital image acquisition unit 63 and detected by imaging.
  • the image acquisition unit 63 may be associated with an image evaluation module 64, which is spatially arranged in a control unit 82 or a control computer 81 (see below) or elsewhere.
  • the necessary connections to the control computer are designated by 83.
  • the laser microdissection system 100 has a laser unit 70 with a laser light source 75.
  • a by the laser light source 75 which is for example a Provided laser beam 77 with laser beam axis b is deflected in a incident light device, which is indicated here at 76 as a whole, at a first deflection mirror 71 and a second deflection mirror 72 and focused by the objective 41 on the sample 51.
  • the location at which the laser beam 77 impinges on the sample 51 in the object plane, and thus also in the sample area, can basically be set in different ways.
  • a manual adjusting device 31 may be provided by means of which the microscope stage 30 designed as a cross table can be adjusted in the x and y directions (that is to say, perpendicularly or parallel to the plane of the paper).
  • electromechanical adjusting means can also be provided which can be activated, for example, by a control unit 82 or whose position can be detected by the control unit 82.
  • the control unit 82 may also control any other motorized functions of the laser microdissection system 100, and in particular provide an interface to an external control computer 81, which may be connected via corresponding connections 83.
  • the control unit 82 or the control computer 81 can also evaluate data obtained, for example, by the image evaluation module 64. By way of example, a sequence of tissue layers or other structures of the sample 51 can thereby be recognized.
  • a laser deflection device 73 can be provided.
  • the laser beam 77 can be deflected relative to an optical axis c extending between the first deflection mirror 71 and the second deflection mirror 72.
  • the laser beam can therefore impinge on the second deflection mirror 72 at different positions, which can be embodied, for example, as a dichromatic divider, and is therefore also focused at different positions on the sample 51 in the object plane.
  • a deflection by means of a laser deflection device 73 is shown in detail in EP 1 276 586 B1. It should be emphasized that here different possibilities for deflecting a laser beam 77 or for positioning the sample 51 in the object plane relative to the Laser beam 77 can be used. The invention is not limited to the illustrated example.
  • the laser deflection device 73 has two solid glass wedge plates 731, which are inclined relative to the optical axis c and are rotatable independently of each other about the optical axis c.
  • the wedge plates 731 are mounted with ball bearings 732.
  • Each of the wedge plates is connected to a gear 733.
  • the gears 733 can each be rotated by means of actuators 734 was-the, which can be acted upon with corresponding drive signals and drive the gears 733 accordingly.
  • the rotators may have position sensors 735 (shown here only on the right actuator 734). A position detected by the position sensors 735 may be transmitted to the control unit 82.
  • At least part of a sample 51 located on the object carrier 52 can be cut out by means of a laser microdissection method.
  • the sample 51 is visualized or the corresponding image of the sample 51 is fed to an image processing system for automatic processing.
  • a sample region of interest may be made visible for example by staining or made usable for image processing.
  • This sample area should be made available as a dissectate for further analysis.
  • the sample area is surrounded, for example, with an individual cutting line, this cutting line imaging the object contour of the sample area.
  • the cutting line may be chosen to be spaced apart from the actual sample area by a certain amount in order not to destroy the sample area in the edge region due to the finite diameter of the laser focus during cutting.
  • the laser beam 77 is displaced by means of the deflection device 73 in accordance with the predetermined individual cutting line by means of the laser dissection device 100 shown in FIG. 1, so that the laser beam focus describes the section line.
  • the Laserablenkvor-direction 73 by means of Controlled accordingly control unit 82 (see the above explanations).
  • the cut microdissect falls by gravity into a laser microdissection collecting tank 53 (not shown in FIG. 1).
  • FIG. 2 very schematically shows only the essential components of the laser microdissection system 100 from FIG. 1, namely the microscope objective 41 used to focus the laser beam 77 and the focused laser beam 77, the focus of which is directed essentially onto the sample 51.
  • the sample holder is designated 52.
  • the laser microdissection collecting container is arranged below the sample 51 and denoted by 53. Also, this collecting container 53 is shown only very schematically in order to illustrate the basic principles of the present invention.
  • the sample 51 is usually located on a support membrane 54, which in turn is held by the sample holder 52.
  • the contamination protection device is designated 500 in this exemplary embodiment. It has a contamination protection plate 550 with an opening 551.
  • the plate is, for example, a 2 mm thick plastic plate or a correspondingly thin sheet metal.
  • the tensioning device for tensioning the contamination protection plate 550 is generally designated 510.
  • the contamination protection plate 550 is attached to two suspensions 560 and 570.
  • an adjustment device generally indicated 540, the contamination protection plate 550 can be adjusted in a (for example, x-direction) main direction of extension of the plate 550, i. be moved in this direction.
  • the clamping device 510 the plate 550 is tensioned in the same direction in this embodiment.
  • adjusting devices 540 and clamping device 510 are combined with one another.
  • the suspension 560 is mounted on a screw adjustment 580 and the suspension 570 on a screw adjustment 590.
  • the contamination protection plate 550 can be moved and adjusted in said extension or spatial direction.
  • This adjusting device allows the opening 551 of the contamination protection plate relative to the position of a collecting container. ters 53 to align exactly.
  • the part 590 of the screw adjustment is based on a resilient element 520, here a spring, the tensioning device 510, so that in each selected position of the plate 550, the spring force of the spring 520 spans this plate 550 in said direction of extent.
  • the embodiment of a contamination protection device 500 shown in FIG. 2 makes it possible to minimize the distance between collecting container 53, more precisely between collecting holder 530, and sample holder 52 in order to even better prevent possible contamination and to further increase the drop height of the laser microdissection into collecting container 53 to reduce.
  • non-active collecting container which are located next to the active collecting container 53, optimally covered.
  • the diameter of the opening 551 can be limited to the minimum necessary diameter, which corresponds to the maximum field of view of the available lenses 41, 42.

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un dispositif de protection contre les contaminations (500) pour un système de microdissection laser (100) qui découpe, à partir d'un échantillon (51) retenu sur un porte-échantillon (52), un échantillon de microdissection laser à l'aide d'un faisceau laser (77) et collecte cet échantillon de microdissection dans un récipient de collecte d'échantillon de microdissection laser (53). Le dispositif de protection contre les contaminations (500) comporte une plaque de protection contre les contaminations (550) dotée d'une ouverture (551), et comprend au moins un dispositif de serrage (510) servant à serrer la plaque de protection contre les contaminations (550). Le dispositif de protection contre les contaminations (500) est conçu de manière à disposer la plaque de protection contre les contaminations (550) entre le porte-échantillon (52) et un support de collecte (530) pour le récipient de collecte d'échantillon de microdissection laser (53) du système de microdissection laser (100) de telle sorte que l'ouverture (551) de la plaque de protection contre les contaminations (550) se situe dans la zone de l'ouverture du récipient de collecte (53). L'invention concerne également un système de microdissection laser (100) correspondant comprenant un tel dispositif de protection contre les contaminations (500) doté d'au moins un dispositif de serrage (510) servant à serrer la plaque de protection contre les contaminations (550).
PCT/EP2017/063572 2016-06-28 2017-06-02 Dispositif de protection contre les contaminations pour un système de microdissection laser et système de microdissection laser WO2018001679A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102016111781.1A DE102016111781B3 (de) 2016-06-28 2016-06-28 Kontaminationsschutzeinrichtung für ein Lasermikrodissektionssystem und Lasermikrodissektionssystem
DE102016111781.1 2016-06-28

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WO2018001679A1 true WO2018001679A1 (fr) 2018-01-04

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN111449621A (zh) * 2020-04-30 2020-07-28 山东省眼科医院 一种裂隙灯显微镜

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Publication number Priority date Publication date Assignee Title
DE29723120U1 (de) * 1996-02-05 1998-05-14 P A L M Gmbh Vorrichtung zur berührungslosen Mikroinjektion sowie zum Sortieren und zur Gewinnung von planar ausgebrachten biologischen Objekten mit Laserstrahlen
DE20111939U1 (de) * 2001-07-15 2002-11-21 Kölble, Konrad, Dr. Dr., 13465 Berlin Mikroskopiereinrichtung und Objektträger zur Mikrodissektion
EP1276586B1 (fr) 2000-04-13 2005-08-10 Leica Microsystems Wetzlar GmbH Dispositif de microdissection laser

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DE10018251C2 (de) * 2000-04-13 2003-08-14 Leica Microsystems Laserschneid-Vorrichtung mit Mikroskop
DE10057292C2 (de) * 2000-11-17 2003-02-13 Leica Microsystems Vorrichtung zum Aufnehmen von Mirodissektaten
DE102013209881A1 (de) * 2013-05-28 2014-12-04 Leica Microsystems Cms Gmbh Lasermikrodissektionssystem mit Visualisierungseinrichtung, Visualisierungseinrichtung für Lasermikrodissektionssystem und Verfahren zur Lasermikrodissektion
DE102013209880A1 (de) * 2013-05-28 2014-12-04 Leica Microsystems Cms Gmbh Verfahren zur Lasermikrodissektion und Lasermikrodissektionssystem
DE102014202860B4 (de) * 2014-02-17 2016-12-29 Leica Microsystems Cms Gmbh Bereitstellen von Probeninformationen mit einem Lasermikrodissektionssystem

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Publication number Priority date Publication date Assignee Title
DE29723120U1 (de) * 1996-02-05 1998-05-14 P A L M Gmbh Vorrichtung zur berührungslosen Mikroinjektion sowie zum Sortieren und zur Gewinnung von planar ausgebrachten biologischen Objekten mit Laserstrahlen
EP1276586B1 (fr) 2000-04-13 2005-08-10 Leica Microsystems Wetzlar GmbH Dispositif de microdissection laser
DE20111939U1 (de) * 2001-07-15 2002-11-21 Kölble, Konrad, Dr. Dr., 13465 Berlin Mikroskopiereinrichtung und Objektträger zur Mikrodissektion

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111449621A (zh) * 2020-04-30 2020-07-28 山东省眼科医院 一种裂隙灯显微镜

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