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WO2009006300A3 - Production de lignées cellulaires pour la fabrication d'adénovirus - Google Patents

Production de lignées cellulaires pour la fabrication d'adénovirus Download PDF

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Publication number
WO2009006300A3
WO2009006300A3 PCT/US2008/068576 US2008068576W WO2009006300A3 WO 2009006300 A3 WO2009006300 A3 WO 2009006300A3 US 2008068576 W US2008068576 W US 2008068576W WO 2009006300 A3 WO2009006300 A3 WO 2009006300A3
Authority
WO
WIPO (PCT)
Prior art keywords
adenovirus
adenoviral
cell lines
manufacturing
cells
Prior art date
Application number
PCT/US2008/068576
Other languages
English (en)
Other versions
WO2009006300A2 (fr
Inventor
Carlos Estuardo
Douglas Jolly
Original Assignee
Advantagene Inc
Carlos Estuardo
Douglas Jolly
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advantagene Inc, Carlos Estuardo, Douglas Jolly filed Critical Advantagene Inc
Publication of WO2009006300A2 publication Critical patent/WO2009006300A2/fr
Publication of WO2009006300A3 publication Critical patent/WO2009006300A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • C12N2710/10352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention porte sur un système de vecteur adénoviral pour la croissance d'un adénovirus déficient en E1A/E1B qui a produit un adénovirus réplicatif (RCA) à une vitesse significativement inférieure à celle observée avec des cellules 293. L'invention porte également sur des procédés pour produire un adénovirus avec des taux réduits de RCA par comparaison avec les cellules 293, l'adénovirus déficient étant développé dans une lignée cellulaire contenant des séquences codantes pour E1A et E1B d'adénovirus qui conservent des zones d'homologie significatives mais limitées aux extrémités à la fois 5' et 3' de la délétion E1 dans le vecteur défectueux. L'invention porte également sur des lignées cellulaires spécifiques utiles dans ce système de production adénoviral et sur des vecteurs et des préparations pharmaceutiques produites avec le système.
PCT/US2008/068576 2007-06-28 2008-06-27 Production de lignées cellulaires pour la fabrication d'adénovirus WO2009006300A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93738007P 2007-06-28 2007-06-28
US60/937,380 2007-06-28

Publications (2)

Publication Number Publication Date
WO2009006300A2 WO2009006300A2 (fr) 2009-01-08
WO2009006300A3 true WO2009006300A3 (fr) 2009-03-05

Family

ID=40226783

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/068576 WO2009006300A2 (fr) 2007-06-28 2008-06-27 Production de lignées cellulaires pour la fabrication d'adénovirus

Country Status (1)

Country Link
WO (1) WO2009006300A2 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001044280A2 (fr) * 1999-12-14 2001-06-21 Genovo, Inc. Methodes et compositions destinees a la fabrication d'adenovirus incompetents pour la replication
US20030040100A1 (en) * 2001-07-23 2003-02-27 Genvec, Inc. Cell for the propagation of adenoviral vectors
US20060063259A1 (en) * 2004-09-21 2006-03-23 Graham Frank L Production of adenovirus vectors with reduced levels of replication competent adenovirus contamination
WO2006060089A2 (fr) * 2004-10-25 2006-06-08 Biogen Idec Ma Inc. Nouveaux procedes permettant de produire des preparations de vecteurs adenoviraux presentant une contamination reduite par des adenovirus replicatifs, et nouveaux vecteurs adenoviraux et preparations associees

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001044280A2 (fr) * 1999-12-14 2001-06-21 Genovo, Inc. Methodes et compositions destinees a la fabrication d'adenovirus incompetents pour la replication
US20030040100A1 (en) * 2001-07-23 2003-02-27 Genvec, Inc. Cell for the propagation of adenoviral vectors
US20060063259A1 (en) * 2004-09-21 2006-03-23 Graham Frank L Production of adenovirus vectors with reduced levels of replication competent adenovirus contamination
WO2006060089A2 (fr) * 2004-10-25 2006-06-08 Biogen Idec Ma Inc. Nouveaux procedes permettant de produire des preparations de vecteurs adenoviraux presentant une contamination reduite par des adenovirus replicatifs, et nouveaux vecteurs adenoviraux et preparations associees

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MARZIO G ET AL.: "A replication-competent adenovirus assay for E1-deleted Ad35 vectors produced in PER.C6 cells.", VACCINE, vol. 25, no. 12, 8 March 2007 (2007-03-08), pages 2228 - 2237, XP005886441 *

Also Published As

Publication number Publication date
WO2009006300A2 (fr) 2009-01-08

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