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WO2009006300A3 - Production cell lines for adenoviral manufacturing - Google Patents

Production cell lines for adenoviral manufacturing Download PDF

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Publication number
WO2009006300A3
WO2009006300A3 PCT/US2008/068576 US2008068576W WO2009006300A3 WO 2009006300 A3 WO2009006300 A3 WO 2009006300A3 US 2008068576 W US2008068576 W US 2008068576W WO 2009006300 A3 WO2009006300 A3 WO 2009006300A3
Authority
WO
WIPO (PCT)
Prior art keywords
adenovirus
adenoviral
cell lines
manufacturing
cells
Prior art date
Application number
PCT/US2008/068576
Other languages
French (fr)
Other versions
WO2009006300A2 (en
Inventor
Carlos Estuardo
Douglas Jolly
Original Assignee
Advantagene Inc
Carlos Estuardo
Douglas Jolly
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advantagene Inc, Carlos Estuardo, Douglas Jolly filed Critical Advantagene Inc
Publication of WO2009006300A2 publication Critical patent/WO2009006300A2/en
Publication of WO2009006300A3 publication Critical patent/WO2009006300A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • C12N2710/10352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

An adenoviral vector system for growth of E1A/E1B deficient adenovirus that has produces replication competent adenovirus (RCA) at a rate significantly below that seen with 293 cells, are provided. Methods for producing adenovirus with reduced levels of RCA compared to 293 cells are also provided, wherein the deficient adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B that retain significant but limited areas of homology to both the 5' and 3' ends of the E1 deletion in the defective vector. Also provided are specific cell lines useful in this adenoviral production system and vectors and pharmaceutical preparations produced with the system.
PCT/US2008/068576 2007-06-28 2008-06-27 Production cell lines for adenoviral manufacturing WO2009006300A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93738007P 2007-06-28 2007-06-28
US60/937,380 2007-06-28

Publications (2)

Publication Number Publication Date
WO2009006300A2 WO2009006300A2 (en) 2009-01-08
WO2009006300A3 true WO2009006300A3 (en) 2009-03-05

Family

ID=40226783

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/068576 WO2009006300A2 (en) 2007-06-28 2008-06-27 Production cell lines for adenoviral manufacturing

Country Status (1)

Country Link
WO (1) WO2009006300A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001044280A2 (en) * 1999-12-14 2001-06-21 Genovo, Inc. Methods and compositions for the manufacture of replication incompetent adenovirus
US20030040100A1 (en) * 2001-07-23 2003-02-27 Genvec, Inc. Cell for the propagation of adenoviral vectors
US20060063259A1 (en) * 2004-09-21 2006-03-23 Graham Frank L Production of adenovirus vectors with reduced levels of replication competent adenovirus contamination
WO2006060089A2 (en) * 2004-10-25 2006-06-08 Biogen Idec Ma Inc. Novel methods for producing adenoviral vector preparations with reduced replication-competent adenovirus contamination and novel adenoviral vectors and preparations

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001044280A2 (en) * 1999-12-14 2001-06-21 Genovo, Inc. Methods and compositions for the manufacture of replication incompetent adenovirus
US20030040100A1 (en) * 2001-07-23 2003-02-27 Genvec, Inc. Cell for the propagation of adenoviral vectors
US20060063259A1 (en) * 2004-09-21 2006-03-23 Graham Frank L Production of adenovirus vectors with reduced levels of replication competent adenovirus contamination
WO2006060089A2 (en) * 2004-10-25 2006-06-08 Biogen Idec Ma Inc. Novel methods for producing adenoviral vector preparations with reduced replication-competent adenovirus contamination and novel adenoviral vectors and preparations

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MARZIO G ET AL.: "A replication-competent adenovirus assay for E1-deleted Ad35 vectors produced in PER.C6 cells.", VACCINE, vol. 25, no. 12, 8 March 2007 (2007-03-08), pages 2228 - 2237, XP005886441 *

Also Published As

Publication number Publication date
WO2009006300A2 (en) 2009-01-08

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