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WO2009041113A1 - Fc受容体遺伝子導入ＮＫ細胞、その作製方法、及びこれを用いた抗体依存性細胞障害のアッセイ方法 - Google Patents

Fc受容体遺伝子導入ＮＫ細胞、その作製方法、及びこれを用いた抗体依存性細胞障害のアッセイ方法 Download PDF

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Publication number
WO2009041113A1
WO2009041113A1 PCT/JP2008/058670 JP2008058670W WO2009041113A1 WO 2009041113 A1 WO2009041113 A1 WO 2009041113A1 JP 2008058670 W JP2008058670 W JP 2008058670W WO 2009041113 A1 WO2009041113 A1 WO 2009041113A1
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Prior art keywords
cell
same
receptor gene
antibody
assay
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Application number
PCT/JP2008/058670
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English (en)
French (fr)
Inventor
Natsuhiko Sugimura
Yuji Mishima
Kiyohiko Hatake
Original Assignee
Olympus Corporation
Japanese Foundation For Cancer Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Olympus Corporation, Japanese Foundation For Cancer Research filed Critical Olympus Corporation
Priority to JP2009534209A priority Critical patent/JP5282040B2/ja
Publication of WO2009041113A1 publication Critical patent/WO2009041113A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

本発明は、健常人ボランティアや被験者に負担をかけることなく、ＡＤＣＣアッセイ用の均質なエフェクタ細胞を大量に得ること、及びこれを利用したＡＤＣＣアッセイ系を構築することを目的とする。本発明によれば、細胞表面にＦｃ受容体を発現した、継代培養可能なエフェクタ細胞株が樹立される。より具体的には、組換えウイルスベクタによりＦｃ受容体遺伝子が細胞の染色体に組み込まれた、ＮＫ細胞由来の細胞株が樹立される。
PCT/JP2008/058670 2007-09-28 2008-05-09 Fc受容体遺伝子導入ＮＫ細胞、その作製方法、及びこれを用いた抗体依存性細胞障害のアッセイ方法 WO2009041113A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009534209A JP5282040B2 (ja) 2007-09-28 2008-05-09 Ｆｃ受容体遺伝子導入ＮＫ細胞を用いた抗体依存性細胞傷害のアッセイ方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007254099 2007-09-28
JP2007-254099 2007-09-28

Publications (1)

Publication Number Publication Date
WO2009041113A1 true WO2009041113A1 (ja) 2009-04-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/058670 WO2009041113A1 (ja) 2007-09-28 2008-05-09 Fc受容体遺伝子導入ＮＫ細胞、その作製方法、及びこれを用いた抗体依存性細胞障害のアッセイ方法

Country Status (2)

Country Link
JP (1) JP5282040B2 (ja)
WO (1) WO2009041113A1 (ja)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013519360A (ja) * 2010-02-11 2013-05-30 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト ３ｄａｄｃｃｎｋｆａｃｓアッセイ
JP2015223143A (ja) * 2014-05-29 2015-12-14 国立大学法人富山大学 Ｔｃｒの細胞傷害活性誘導能を評価するためのｎｋ細胞株、およびその作製方法
CN110546265A (zh) * 2017-02-09 2019-12-06 因达普塔治疗公司 工程化自然杀伤(nk)细胞及其组合物和方法
CN112980851A (zh) * 2021-03-03 2021-06-18 上海奥浦迈生物科技股份有限公司 高效检测融合蛋白/抗体Fc段不能介导ADCC和CDC活性的方法
JP2021522839A (ja) * 2018-05-14 2021-09-02 インダプタ セラピューティクス インコーポレイテッド 増強された抗体指向性免疫応答を有するヒトナチュラルキラー細胞のサブセット
US12152253B2 (en) 2012-04-18 2024-11-26 Board Of Trustees Of Michigan State University Natural killer cells with enhanced immune response

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7426661B2 (ja) * 2018-12-17 2024-02-02 株式会社カネカ 特定抗原特異的抗体を産生する細胞のスクリーニング方法

Citations (2)

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JP2004147565A (ja) * 2002-10-30 2004-05-27 Japan Science & Technology Agency 不死化ナチュラルキラー細胞株
US20060292156A1 (en) * 2004-07-10 2006-12-28 Campbell Kerry S Genetically modified human natural killer cell lines

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MXPA03011805A (es) * 2001-07-06 2004-07-01 Senomyx Inc Expresion del canal de compuerta de nucleotido ciclico olfatorio humano funcional en celulas hospedadoras recombinantes y uso de los mismos en ensayos basados en celulas para identificar moduladores del olfato.
JP2006526390A (ja) * 2003-01-13 2006-11-24 エモリー ユニバーシティ 正常細胞およびがん細胞における遺伝子発現を検出する方法
JP2007053926A (ja) * 2005-08-23 2007-03-08 Hamamatsu Kagaku Gijutsu Kenkyu Shinkokai 光毒性の高感度検出方法及び光毒性検出用キット

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004147565A (ja) * 2002-10-30 2004-05-27 Japan Science & Technology Agency 不死化ナチュラルキラー細胞株
US20060292156A1 (en) * 2004-07-10 2006-12-28 Campbell Kerry S Genetically modified human natural killer cell lines

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LIAO F. ET AL.: "Cross-linking of FcyRIIIA on natural killer cells results in tyrosine phosphorylation of PLC-yl and PLC-y2.", THE JOURNAL OF IMMUNOLOGY, vol. 150, no. 7, 1993, pages 2668 - 2674 *
SUCK G. ET AL.: "KHYG-1, a model for the study of enhanced natural killer cell cytotoxicity.", EXPERIMENTAL HEMATOLOGY, vol. 33, no. 10, 2005, pages 1160 - 1171, XP005108371, DOI: doi:10.1016/j.exphem.2005.06.024 *
YUJI MISHIMA ET AL.: "Establishment of reproducible ADCC assay system.", 66TH PROCEEDINGS OF THE JAPANESE CANCER ASSOCIATION, vol. 340, 25 August 2007 (2007-08-25), pages 750 *
YUJI MISHIMA: "Kotai Iyaku no Hotai Izonsei, Kotai Izonsei Satsusaibo Koka", PHARMA MEDICA, vol. 25, no. 3, 10 March 2007 (2007-03-10), pages 9 - 13 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013519360A (ja) * 2010-02-11 2013-05-30 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト ３ｄａｄｃｃｎｋｆａｃｓアッセイ
US10330669B2 (en) 2010-02-11 2019-06-25 Hoffmann-La Roche Inc. 3D ADCC NK FACS assay
US12152253B2 (en) 2012-04-18 2024-11-26 Board Of Trustees Of Michigan State University Natural killer cells with enhanced immune response
JP2015223143A (ja) * 2014-05-29 2015-12-14 国立大学法人富山大学 Ｔｃｒの細胞傷害活性誘導能を評価するためのｎｋ細胞株、およびその作製方法
CN110546265A (zh) * 2017-02-09 2019-12-06 因达普塔治疗公司 工程化自然杀伤(nk)细胞及其组合物和方法
JP2020507349A (ja) * 2017-02-09 2020-03-12 インダプタ セラピューティクス インコーポレイテッド 操作されたナチュラルキラー（ｎｋ）細胞ならびにその組成物および方法
US11920156B2 (en) 2017-02-09 2024-03-05 Indapta Therapeutics, Inc. Engineered natural killer (NK) cells and compositions and methods thereof
JP2021522839A (ja) * 2018-05-14 2021-09-02 インダプタ セラピューティクス インコーポレイテッド 増強された抗体指向性免疫応答を有するヒトナチュラルキラー細胞のサブセット
CN112980851A (zh) * 2021-03-03 2021-06-18 上海奥浦迈生物科技股份有限公司 高效检测融合蛋白/抗体Fc段不能介导ADCC和CDC活性的方法

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JP5282040B2 (ja) 2013-09-04

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