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WO2009041113A1 - NK CELL HAVING Fc RECEPTOR GENE INTRODUCED THEREIN, METHOD FOR PRODUCTION OF THE SAME, AND METHOD FOR ASSAY ON ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY USING THE SAME - Google Patents

NK CELL HAVING Fc RECEPTOR GENE INTRODUCED THEREIN, METHOD FOR PRODUCTION OF THE SAME, AND METHOD FOR ASSAY ON ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY USING THE SAME Download PDF

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Publication number
WO2009041113A1
WO2009041113A1 PCT/JP2008/058670 JP2008058670W WO2009041113A1 WO 2009041113 A1 WO2009041113 A1 WO 2009041113A1 JP 2008058670 W JP2008058670 W JP 2008058670W WO 2009041113 A1 WO2009041113 A1 WO 2009041113A1
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WO
WIPO (PCT)
Prior art keywords
cell
same
receptor gene
antibody
assay
Prior art date
Application number
PCT/JP2008/058670
Other languages
French (fr)
Japanese (ja)
Inventor
Natsuhiko Sugimura
Yuji Mishima
Kiyohiko Hatake
Original Assignee
Olympus Corporation
Japanese Foundation For Cancer Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corporation, Japanese Foundation For Cancer Research filed Critical Olympus Corporation
Priority to JP2009534209A priority Critical patent/JP5282040B2/en
Publication of WO2009041113A1 publication Critical patent/WO2009041113A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The object is: to produce uniform effector cells for an ADCC assay in large quantity without taking a toll on the body of a healthy volunteer or a subject; and to construct an ADCC assay system by utilizing the effector cells. An effector cell strain can be established, which has an Fc receptor expressed on the surface of the cell and can be continuously cultured (sub-cultured). More specifically, a cell strain derived from an NK cell can be established, which has an Fc receptor gene integrated into the chromosome of the cell by using a recombinant viral vector.
PCT/JP2008/058670 2007-09-28 2008-05-09 NK CELL HAVING Fc RECEPTOR GENE INTRODUCED THEREIN, METHOD FOR PRODUCTION OF THE SAME, AND METHOD FOR ASSAY ON ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY USING THE SAME WO2009041113A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009534209A JP5282040B2 (en) 2007-09-28 2008-05-09 Method for assaying antibody-dependent cytotoxicity using Fc receptor gene-transferred NK cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007254099 2007-09-28
JP2007-254099 2007-09-28

Publications (1)

Publication Number Publication Date
WO2009041113A1 true WO2009041113A1 (en) 2009-04-02

Family

ID=40511014

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/058670 WO2009041113A1 (en) 2007-09-28 2008-05-09 NK CELL HAVING Fc RECEPTOR GENE INTRODUCED THEREIN, METHOD FOR PRODUCTION OF THE SAME, AND METHOD FOR ASSAY ON ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY USING THE SAME

Country Status (2)

Country Link
JP (1) JP5282040B2 (en)
WO (1) WO2009041113A1 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013519360A (en) * 2010-02-11 2013-05-30 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト 3DADCCNKFACS assay
JP2015223143A (en) * 2014-05-29 2015-12-14 国立大学法人富山大学 Nk cell strain for evaluating cytotoxic activity-inducing potency of tcr, and production method thereof
CN110546265A (en) * 2017-02-09 2019-12-06 因达普塔治疗公司 Engineered Natural Killer (NK) cells and compositions and methods thereof
CN112980851A (en) * 2021-03-03 2021-06-18 上海奥浦迈生物科技股份有限公司 Method for efficiently detecting fusion protein/antibody Fc segment incapable of mediating ADCC (ADCC-mediated cancer cell mediated cytotoxicity) and CDC (CDC) activities
JP2021522839A (en) * 2018-05-14 2021-09-02 インダプタ セラピューティクス インコーポレイテッド A subset of human natural killer cells with enhanced antibody-directed immune response
US12152253B2 (en) 2012-04-18 2024-11-26 Board Of Trustees Of Michigan State University Natural killer cells with enhanced immune response

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020129838A1 (en) * 2018-12-17 2020-06-25 株式会社カネカ Screening method for cells producing specific antigen-specific antibody

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004147565A (en) * 2002-10-30 2004-05-27 Japan Science & Technology Agency Immortalized natural killer cell line
US20060292156A1 (en) * 2004-07-10 2006-12-28 Campbell Kerry S Genetically modified human natural killer cell lines

Family Cites Families (3)

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IL159242A0 (en) * 2001-07-06 2004-06-01 Senomyx Inc Expression of functional human olfactory cyclic nucleotide gated (cng) channel in recombinant host cells and use thereof in cell based assays to identify smell modulators
CA2512956A1 (en) * 2003-01-13 2004-07-29 Emory University Methods of detecting gene expression in normal and cancerous cells
JP2007053926A (en) * 2005-08-23 2007-03-08 Hamamatsu Kagaku Gijutsu Kenkyu Shinkokai Highly sensitive method for detecting phototoxicity, and phototoxicity detection kit

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004147565A (en) * 2002-10-30 2004-05-27 Japan Science & Technology Agency Immortalized natural killer cell line
US20060292156A1 (en) * 2004-07-10 2006-12-28 Campbell Kerry S Genetically modified human natural killer cell lines

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LIAO F. ET AL.: "Cross-linking of FcyRIIIA on natural killer cells results in tyrosine phosphorylation of PLC-yl and PLC-y2.", THE JOURNAL OF IMMUNOLOGY, vol. 150, no. 7, 1993, pages 2668 - 2674 *
SUCK G. ET AL.: "KHYG-1, a model for the study of enhanced natural killer cell cytotoxicity.", EXPERIMENTAL HEMATOLOGY, vol. 33, no. 10, 2005, pages 1160 - 1171, XP005108371, DOI: doi:10.1016/j.exphem.2005.06.024 *
YUJI MISHIMA ET AL.: "Establishment of reproducible ADCC assay system.", 66TH PROCEEDINGS OF THE JAPANESE CANCER ASSOCIATION, vol. 340, 25 August 2007 (2007-08-25), pages 750 *
YUJI MISHIMA: "Kotai Iyaku no Hotai Izonsei, Kotai Izonsei Satsusaibo Koka", PHARMA MEDICA, vol. 25, no. 3, 10 March 2007 (2007-03-10), pages 9 - 13 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013519360A (en) * 2010-02-11 2013-05-30 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト 3DADCCNKFACS assay
US10330669B2 (en) 2010-02-11 2019-06-25 Hoffmann-La Roche Inc. 3D ADCC NK FACS assay
US12152253B2 (en) 2012-04-18 2024-11-26 Board Of Trustees Of Michigan State University Natural killer cells with enhanced immune response
JP2015223143A (en) * 2014-05-29 2015-12-14 国立大学法人富山大学 Nk cell strain for evaluating cytotoxic activity-inducing potency of tcr, and production method thereof
CN110546265A (en) * 2017-02-09 2019-12-06 因达普塔治疗公司 Engineered Natural Killer (NK) cells and compositions and methods thereof
JP2020507349A (en) * 2017-02-09 2020-03-12 インダプタ セラピューティクス インコーポレイテッド Engineered natural killer (NK) cells and compositions and methods thereof
US11920156B2 (en) 2017-02-09 2024-03-05 Indapta Therapeutics, Inc. Engineered natural killer (NK) cells and compositions and methods thereof
JP2021522839A (en) * 2018-05-14 2021-09-02 インダプタ セラピューティクス インコーポレイテッド A subset of human natural killer cells with enhanced antibody-directed immune response
CN112980851A (en) * 2021-03-03 2021-06-18 上海奥浦迈生物科技股份有限公司 Method for efficiently detecting fusion protein/antibody Fc segment incapable of mediating ADCC (ADCC-mediated cancer cell mediated cytotoxicity) and CDC (CDC) activities

Also Published As

Publication number Publication date
JP5282040B2 (en) 2013-09-04
JPWO2009041113A1 (en) 2011-01-20

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