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WO2008136321A1 - キャピラリー電気泳動法によるヘモグロビンの分析方法およびそれに用いる添加剤 - Google Patents

キャピラリー電気泳動法によるヘモグロビンの分析方法およびそれに用いる添加剤 Download PDF

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Publication number
WO2008136321A1
WO2008136321A1 PCT/JP2008/057825 JP2008057825W WO2008136321A1 WO 2008136321 A1 WO2008136321 A1 WO 2008136321A1 JP 2008057825 W JP2008057825 W JP 2008057825W WO 2008136321 A1 WO2008136321 A1 WO 2008136321A1
Authority
WO
WIPO (PCT)
Prior art keywords
electrophoresis
surfactant
sample
capillary tube
buffer
Prior art date
Application number
PCT/JP2008/057825
Other languages
English (en)
French (fr)
Inventor
Yusuke Nakayama
Satoshi Yonehara
Original Assignee
Arkray, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray, Inc. filed Critical Arkray, Inc.
Priority to EP08751963.3A priority Critical patent/EP2144055B1/en
Priority to JP2008535811A priority patent/JP4660653B2/ja
Priority to CN2008800009314A priority patent/CN101548181B/zh
Priority to US12/514,293 priority patent/US8361292B2/en
Publication of WO2008136321A1 publication Critical patent/WO2008136321A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　装置を小型化でき、分析精度が高く、短時間で分析可能なキャピラリー電気泳動法によるヘモグロビンの分析方法を提供する。 　本発明の分析方法は、キャピラリー電気泳動法によるヘモグロビンの分析方法であって、ヘモグロビンを含む試料を準備する試料準備工程と、緩衝液を含むキャピラリー管を準備するキャピラリー管準備工程と、キャピラリー管の緩衝液中に試料を導入し、キャピラリー管の両端に電圧を印加して試料を電気泳動する電気泳動工程とを含み、下記（Ａ）および下記（Ｂ）の少なくとも一方の態様により電気泳動を実施することを特徴とする。 （Ａ）緩衝液中に、下記（ａ）界面活性剤を添加して電気泳動を実施する。 （ａ）界面活性剤：疎水部としてアルキル基を有し、親水部として糖を有する非イオン性界面活性剤 （Ｂ）試料中に、下記（ｂ）界面活性剤を添加して電気泳動を実施する。 （ｂ）界面活性剤：ベタイン型両性界面活性剤  
PCT/JP2008/057825 2007-04-27 2008-04-23 キャピラリー電気泳動法によるヘモグロビンの分析方法およびそれに用いる添加剤 WO2008136321A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP08751963.3A EP2144055B1 (en) 2007-04-27 2008-04-23 Method of analyzing hemoglobin by capillary electrophoresis and additive to be used therein
JP2008535811A JP4660653B2 (ja) 2007-04-27 2008-04-23 キャピラリー電気泳動法によるヘモグロビンの分析方法およびそれに用いる添加剤
CN2008800009314A CN101548181B (zh) 2007-04-27 2008-04-23 利用毛细管电泳法的血红蛋白分析方法及该方法中使用的添加剂
US12/514,293 US8361292B2 (en) 2007-04-27 2008-04-23 Method for analyzing hemoglobin by capillary electrophoresis and additive used therein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007119259 2007-04-27
JP2007-119259 2007-04-27

Publications (1)

Publication Number Publication Date
WO2008136321A1 true WO2008136321A1 (ja) 2008-11-13

Family

ID=39943433

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/057825 WO2008136321A1 (ja) 2007-04-27 2008-04-23 キャピラリー電気泳動法によるヘモグロビンの分析方法およびそれに用いる添加剤

Country Status (5)

Country Link
US (1) US8361292B2 (ja)
EP (1) EP2144055B1 (ja)
JP (1) JP4660653B2 (ja)
CN (1) CN101548181B (ja)
WO (1) WO2008136321A1 (ja)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012063348A (ja) * 2010-08-16 2012-03-29 Arkray Inc ヘモグロビンの分析方法
EP2757368A1 (en) 2013-01-22 2014-07-23 ARKRAY, Inc. Sample analysis method and solution to be used therein, wherein a non-surfactant-type zwitterionic substance is used
JP2016028234A (ja) * 2014-07-09 2016-02-25 アークレイ株式会社 緩衝剤組成物
EP2993467A1 (en) 2014-09-04 2016-03-09 ARKRAY, Inc. Analysis method and analysis system
EP3179243A1 (en) 2015-12-09 2017-06-14 ARKRAY, Inc. Analytical tool for capillary electrophoresis with pressure fluctuation reducer
JP2017106904A (ja) * 2015-12-09 2017-06-15 アークレイ株式会社 分析用具および分析システム
US9841401B2 (en) 2009-04-14 2017-12-12 National Cheng Kung University Capillary electrophoresis method for analyzing collagen

Families Citing this family (2)

* Cited by examiner, † Cited by third party
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WO2008029685A1 (fr) * 2006-09-04 2008-03-13 National Institute Of Advanced Industrial Science And Technology Procédé pour analyse d'échantillon par électrophorèse capillaire
US9017536B2 (en) 2006-12-26 2015-04-28 Sekisui Chemical Co., Ltd. Hemoglobin measurement method and electrophoresis apparatus

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09105739A (ja) * 1995-03-22 1997-04-22 Analis Sa 毛管電気泳動検出および／または分析方法およびユニット
JPH11223624A (ja) * 1997-11-12 1999-08-17 Shino Test:Kk リガンドに結合している物質の測定装置、及び測定方法
JPH11295286A (ja) * 1998-04-10 1999-10-29 Sekisui Chem Co Ltd ヘモグロビン類の測定方法
JP2004077457A (ja) * 2002-06-18 2004-03-11 Asahi Kasei Pharma Kk 糖化タンパク質測定用検量物質および標準測定法
US20040256232A1 (en) 2003-04-09 2004-12-23 Wen Jiang Open tube suitable for separation purposes comprising covalently bonded zwitterionic betaine groups
US20060102478A1 (en) 2004-11-18 2006-05-18 Sebia Method for analyzing haemoglobin by capillary electrophoresis, a kit for capillary electrophoresis, and use of a flow inhibitor in said method

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US5599433A (en) * 1995-01-17 1997-02-04 Beckman Instruments, Inc. Capillary electrophoresis of glycosylated proteins
US5786224A (en) * 1995-06-29 1998-07-28 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
DE19649895A1 (de) 1996-12-02 1998-06-04 Henkel Kgaa Schäumende Körperreinigungsmittel
WO2001006243A1 (fr) * 1999-07-16 2001-01-25 Asahi Kasei Kabushiki Kaisha Procede de mesure d'une substance et reactif a cet usage
JP4524446B2 (ja) 2002-03-31 2010-08-18 株式会社シノテスト 糖脂質抗原の活性化剤及び活性化方法、並びに試料中の抗糖脂質抗体の測定方法及び測定試薬キット
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JP4854570B2 (ja) 2007-04-03 2012-01-18 積水化学工業株式会社 電気泳動用キャピラリー
JP4854525B2 (ja) 2007-01-12 2012-01-18 積水化学工業株式会社 ヘモグロビン類の測定方法
JP4854503B2 (ja) 2006-12-27 2012-01-18 積水化学工業株式会社 安定型ヘモグロビンＡ１ｃの測定方法
JP4854577B2 (ja) 2007-04-16 2012-01-18 積水化学工業株式会社 ヘモグロビン類の測定方法
US9017536B2 (en) * 2006-12-26 2015-04-28 Sekisui Chemical Co., Ltd. Hemoglobin measurement method and electrophoresis apparatus
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Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09105739A (ja) * 1995-03-22 1997-04-22 Analis Sa 毛管電気泳動検出および／または分析方法およびユニット
JPH11223624A (ja) * 1997-11-12 1999-08-17 Shino Test:Kk リガンドに結合している物質の測定装置、及び測定方法
JPH11295286A (ja) * 1998-04-10 1999-10-29 Sekisui Chem Co Ltd ヘモグロビン類の測定方法
JP2004077457A (ja) * 2002-06-18 2004-03-11 Asahi Kasei Pharma Kk 糖化タンパク質測定用検量物質および標準測定法
US20040256232A1 (en) 2003-04-09 2004-12-23 Wen Jiang Open tube suitable for separation purposes comprising covalently bonded zwitterionic betaine groups
US20060102478A1 (en) 2004-11-18 2006-05-18 Sebia Method for analyzing haemoglobin by capillary electrophoresis, a kit for capillary electrophoresis, and use of a flow inhibitor in said method
JP2006145537A (ja) * 2004-11-18 2006-06-08 Sebia キャピラリー電気泳動法によるヘモグロビンの分析方法、キャピラリー電気泳動キット及び該方法における流れ阻害剤の使用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2144055A4

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9841401B2 (en) 2009-04-14 2017-12-12 National Cheng Kung University Capillary electrophoresis method for analyzing collagen
JP2012063348A (ja) * 2010-08-16 2012-03-29 Arkray Inc ヘモグロビンの分析方法
EP2757368A1 (en) 2013-01-22 2014-07-23 ARKRAY, Inc. Sample analysis method and solution to be used therein, wherein a non-surfactant-type zwitterionic substance is used
JP2014160058A (ja) * 2013-01-22 2014-09-04 Arkray Inc 試料の分析方法及びそれに用いる溶液
US9618478B2 (en) 2013-01-22 2017-04-11 Arkray, Inc. Sample analysis method and solution to be used therein
JP2016028234A (ja) * 2014-07-09 2016-02-25 アークレイ株式会社 緩衝剤組成物
US9528963B2 (en) 2014-07-09 2016-12-27 Arkray, Inc. Buffer composition
EP2993467A1 (en) 2014-09-04 2016-03-09 ARKRAY, Inc. Analysis method and analysis system
US10018589B2 (en) 2014-09-04 2018-07-10 Arkray, Inc. Analysis method and analysis system
EP3179243A1 (en) 2015-12-09 2017-06-14 ARKRAY, Inc. Analytical tool for capillary electrophoresis with pressure fluctuation reducer
JP2017106904A (ja) * 2015-12-09 2017-06-15 アークレイ株式会社 分析用具および分析システム
US11187674B2 (en) 2015-12-09 2021-11-30 Arkray, Inc. Analytical tool and analytical system

Also Published As

Publication number Publication date
EP2144055A4 (en) 2013-02-13
CN101548181B (zh) 2012-11-28
CN101548181A (zh) 2009-09-30
EP2144055A1 (en) 2010-01-13
JPWO2008136321A1 (ja) 2010-07-29
US20100032294A1 (en) 2010-02-11
US8361292B2 (en) 2013-01-29
EP2144055B1 (en) 2014-03-12
JP4660653B2 (ja) 2011-03-30

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