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WO2008135047A3 - Controllable promoters for use in fission yeast - Google Patents

Controllable promoters for use in fission yeast Download PDF

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Publication number
WO2008135047A3
WO2008135047A3 PCT/DK2008/050098 DK2008050098W WO2008135047A3 WO 2008135047 A3 WO2008135047 A3 WO 2008135047A3 DK 2008050098 W DK2008050098 W DK 2008050098W WO 2008135047 A3 WO2008135047 A3 WO 2008135047A3
Authority
WO
WIPO (PCT)
Prior art keywords
fission yeast
promoter
vectorsand
nmt
chimeric
Prior art date
Application number
PCT/DK2008/050098
Other languages
French (fr)
Other versions
WO2008135047A2 (en
Inventor
Soeren Kjaerulff
Original Assignee
Univ Koebenhavn
Soeren Kjaerulff
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Koebenhavn, Soeren Kjaerulff filed Critical Univ Koebenhavn
Publication of WO2008135047A2 publication Critical patent/WO2008135047A2/en
Publication of WO2008135047A3 publication Critical patent/WO2008135047A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a nucleic acid comprising a chimeric nmt::lacO promoter that allows control of gene expression in fission yeast. Hence, a gene controlled by the promoter can be turned on and off. Such promoter is useful for protein expression in fission yeast and also for biological studies in fission yeast. Other aspects of theinvention are vectorsand a kit comprising the vectorsand a method of inducing synthesis of a pre-determined RNA, said pre-determined RNA being under the control of the chimeric nmt::lacO promoter.
PCT/DK2008/050098 2007-05-02 2008-04-30 Controllable promoters for use in fission yeast WO2008135047A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200700663 2007-05-02
DKPA200700663 2007-05-02

Publications (2)

Publication Number Publication Date
WO2008135047A2 WO2008135047A2 (en) 2008-11-13
WO2008135047A3 true WO2008135047A3 (en) 2008-12-31

Family

ID=39790213

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2008/050098 WO2008135047A2 (en) 2007-05-02 2008-04-30 Controllable promoters for use in fission yeast

Country Status (1)

Country Link
WO (1) WO2008135047A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866362A (en) * 1985-03-28 1999-02-02 Chiron Corporation Enhanced purification and expression of insoluble recombinant proteins
US20030040102A1 (en) * 2001-06-05 2003-02-27 Hunt Arthur G. Feedback-regulated expression system and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866362A (en) * 1985-03-28 1999-02-02 Chiron Corporation Enhanced purification and expression of insoluble recombinant proteins
US20030040102A1 (en) * 2001-06-05 2003-02-27 Hunt Arthur G. Feedback-regulated expression system and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BRENT R ET AL: "A BACTERIAL REPRESSOR PROTEIN OR A YEAST TRANSCRIPTIONAL TERMINATOR CAN BLOCK UPSTREAM ACTIVATION OF A YEAST GENE", NATURE (LONDON), vol. 312, no. 5995, 1984, pages 612 - 615, XP009106870, ISSN: 0028-0836 *
IACOVONI J S ET AL: "A new inducible protein expression system in fission yeast based on the glucose-repressed inv1 promoter", GENE, ELSEVIER, AMSTERDAM, NL, vol. 232, no. 1, 17 May 1999 (1999-05-17), pages 53 - 58, XP004166802, ISSN: 0378-1119 *
VERGANI P ET AL: "Complementation of a yeast DELTApkc1 mutant by the Arabidopsis protein ANT", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 400, no. 2, 3 January 1997 (1997-01-03), pages 243 - 246, XP004262051, ISSN: 0014-5793 *

Also Published As

Publication number Publication date
WO2008135047A2 (en) 2008-11-13

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