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WO2008123019A1 - 遺伝子変化の検出方法および検出装置 - Google Patents

遺伝子変化の検出方法および検出装置 Download PDF

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Publication number
WO2008123019A1
WO2008123019A1 PCT/JP2008/050895 JP2008050895W WO2008123019A1 WO 2008123019 A1 WO2008123019 A1 WO 2008123019A1 JP 2008050895 W JP2008050895 W JP 2008050895W WO 2008123019 A1 WO2008123019 A1 WO 2008123019A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
gene
acid amplification
reaction container
change
Prior art date
Application number
PCT/JP2008/050895
Other languages
English (en)
French (fr)
Inventor
Hiromi Sanuki
Shigeru Komiyama
Original Assignee
Olympus Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corporation filed Critical Olympus Corporation
Priority to JP2008533810A priority Critical patent/JPWO2008123019A1/ja
Publication of WO2008123019A1 publication Critical patent/WO2008123019A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本発明は、微量な生体試料に対して効率良く遺伝子変化を検出する自動化に適した技術を提供することを目的とする。本発明の遺伝子変化の検出方法は、生体試料から核酸を抽出する工程と、前記１）工程で得られた核酸溶液を、０．５～５μｌの容量で、温度調整可能な容器台上に設置された、同一反応容器上の異なる位置に分注する工程と、温度調整可能な装置の温度を調整することにより、反応容器上の核酸溶液を乾燥させる工程と、反応容器上の乾燥させた核酸上に、０．５～５μｌの容量で核酸増幅用の試薬を分注する工程と、前記工程で分注した核酸増幅用の試薬上に、３～１０μｌの容量で核酸増幅用溶液の蒸発を防ぐための溶液を分注する工程と、温度調整可能な装置の温度を調整することにより、核酸増幅反応を行なう工程との後、核酸増幅反応後の産物を検出し、遺伝子変化解析を行なうことを特徴とする。
PCT/JP2008/050895 2007-03-05 2008-01-23 遺伝子変化の検出方法および検出装置 WO2008123019A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008533810A JPWO2008123019A1 (ja) 2007-03-05 2008-01-23 遺伝子変化の検出方法および検出装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-054708 2007-03-05
JP2007054708 2007-03-05

Publications (1)

Publication Number Publication Date
WO2008123019A1 true WO2008123019A1 (ja) 2008-10-16

Family

ID=39830522

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050895 WO2008123019A1 (ja) 2007-03-05 2008-01-23 遺伝子変化の検出方法および検出装置

Country Status (2)

Country Link
JP (1) JPWO2008123019A1 (ja)
WO (1) WO2008123019A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011024947A1 (ja) * 2009-08-31 2011-03-03 株式会社ハウステック 成形構造体
WO2011040504A1 (ja) * 2009-09-30 2011-04-07 凸版印刷株式会社 核酸分析装置
JP2012075413A (ja) * 2010-10-05 2012-04-19 Hitachi High-Technologies Corp 核酸検査装置及び容器搬送方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001149097A (ja) * 1999-11-29 2001-06-05 Olympus Optical Co Ltd 自動核酸検査装置
WO2002025289A1 (fr) * 2000-09-18 2002-03-28 I-Card Corporation Ensemble de micro-coupelles et procede permettant d'enfermer hermetiquement des liquides au moyen de cet ensemble
JP2003061684A (ja) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd Ｄｎａの塩基配列決定法
JP2003304899A (ja) * 2002-04-11 2003-10-28 Yoshinobu Baba 核酸の分析方法及び装置
JP2005515792A (ja) * 2002-01-29 2005-06-02 グローバル ジェノミクス アクティエボラーグ 核酸を操作するための方法および手段

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001149097A (ja) * 1999-11-29 2001-06-05 Olympus Optical Co Ltd 自動核酸検査装置
WO2002025289A1 (fr) * 2000-09-18 2002-03-28 I-Card Corporation Ensemble de micro-coupelles et procede permettant d'enfermer hermetiquement des liquides au moyen de cet ensemble
JP2003061684A (ja) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd Ｄｎａの塩基配列決定法
JP2005515792A (ja) * 2002-01-29 2005-06-02 グローバル ジェノミクス アクティエボラーグ 核酸を操作するための方法および手段
JP2003304899A (ja) * 2002-04-11 2003-10-28 Yoshinobu Baba 核酸の分析方法及び装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TOJO H. ET AL.: "A rapid methods for screening transgenic mice by PCR with small amounts of blood", EXP. ANIM., vol. 40, no. 2, 1991, pages 235 - 238 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011024947A1 (ja) * 2009-08-31 2011-03-03 株式会社ハウステック 成形構造体
JP2011053334A (ja) * 2009-08-31 2011-03-17 Housetec Inc 成形構造体
WO2011040504A1 (ja) * 2009-09-30 2011-04-07 凸版印刷株式会社 核酸分析装置
JP2012161340A (ja) * 2009-09-30 2012-08-30 Toppan Printing Co Ltd 核酸分析装置、及び、核酸の分離精製方法
US9267890B2 (en) 2009-09-30 2016-02-23 Toppan Printing Co., Ltd. Nucleic acid analyzer
JP2012075413A (ja) * 2010-10-05 2012-04-19 Hitachi High-Technologies Corp 核酸検査装置及び容器搬送方法

Also Published As

Publication number Publication date
JPWO2008123019A1 (ja) 2010-07-15

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