WO2008123019A1 - Method of detecting change in gene and detection device - Google Patents
Method of detecting change in gene and detection device Download PDFInfo
- Publication number
- WO2008123019A1 WO2008123019A1 PCT/JP2008/050895 JP2008050895W WO2008123019A1 WO 2008123019 A1 WO2008123019 A1 WO 2008123019A1 JP 2008050895 W JP2008050895 W JP 2008050895W WO 2008123019 A1 WO2008123019 A1 WO 2008123019A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- gene
- acid amplification
- reaction container
- change
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 9
- 150000007523 nucleic acids Chemical class 0.000 abstract 9
- 102000039446 nucleic acids Human genes 0.000 abstract 9
- 230000003321 amplification Effects 0.000 abstract 5
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 5
- 239000012472 biological sample Substances 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000001704 evaporation Methods 0.000 abstract 1
- 230000008020 evaporation Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
It is intended to provide a technique suitable for the automated detection whereby a change in a gene in a minute amount of a biological sample can be efficiently detected. Namely, a method of detecting a change in a gene which comprises: the step of extracting a nucleic acid from a biological sample; the step of pipetting 0.5 to 5 μl portions of the nucleic acid solution obtained in the above step 1) into different positions on a single reaction container located on a reaction container table allowing temperature control; the step of drying the nucleic acid solution on the reaction container by controlling the temperature of the unit allowing temperature control; the step of pipetting 0.5 to 5 μl portions of a nucleic acid amplification reagent to the dried nucleic acid on the reaction container; the step of pipetting 3 to 10 μl portions of a solution for preventing the nucleic acid amplification solution from evaporation to the nucleic acid amplification reagent pipetted on the above step; and the step of conducting the nucleic acid amplification reaction by controlling the temperature of the unit allowing temperature control; followed by the detection of the product obtained after the completion of the nucleic acid amplification reaction and the analysis of the change in the gene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008533810A JPWO2008123019A1 (en) | 2007-03-05 | 2008-01-23 | Gene change detection method and detection apparatus |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007-054708 | 2007-03-05 | ||
| JP2007054708 | 2007-03-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2008123019A1 true WO2008123019A1 (en) | 2008-10-16 |
Family
ID=39830522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2008/050895 WO2008123019A1 (en) | 2007-03-05 | 2008-01-23 | Method of detecting change in gene and detection device |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPWO2008123019A1 (en) |
| WO (1) | WO2008123019A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011024947A1 (en) * | 2009-08-31 | 2011-03-03 | 株式会社ハウステック | Molded structure |
| WO2011040504A1 (en) * | 2009-09-30 | 2011-04-07 | 凸版印刷株式会社 | Nucleic acid analyzer |
| JP2012075413A (en) * | 2010-10-05 | 2012-04-19 | Hitachi High-Technologies Corp | Apparatus for inspecting nucleic acid and method of conveying container |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001149097A (en) * | 1999-11-29 | 2001-06-05 | Olympus Optical Co Ltd | Automatic nucleic acid examination apparatus |
| WO2002025289A1 (en) * | 2000-09-18 | 2002-03-28 | I-Card Corporation | Micro well array and method of sealing liquid using the micro well array |
| JP2003061684A (en) * | 2001-06-11 | 2003-03-04 | Nippon Genetech Co Ltd | Method for dna base sequence determination |
| JP2003304899A (en) * | 2002-04-11 | 2003-10-28 | Yoshinobu Baba | Method and apparatus for analyzing nucleic acid |
| JP2005515792A (en) * | 2002-01-29 | 2005-06-02 | グローバル ジェノミクス アクティエボラーグ | Methods and means for manipulating nucleic acids |
-
2008
- 2008-01-23 WO PCT/JP2008/050895 patent/WO2008123019A1/en active Application Filing
- 2008-01-23 JP JP2008533810A patent/JPWO2008123019A1/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001149097A (en) * | 1999-11-29 | 2001-06-05 | Olympus Optical Co Ltd | Automatic nucleic acid examination apparatus |
| WO2002025289A1 (en) * | 2000-09-18 | 2002-03-28 | I-Card Corporation | Micro well array and method of sealing liquid using the micro well array |
| JP2003061684A (en) * | 2001-06-11 | 2003-03-04 | Nippon Genetech Co Ltd | Method for dna base sequence determination |
| JP2005515792A (en) * | 2002-01-29 | 2005-06-02 | グローバル ジェノミクス アクティエボラーグ | Methods and means for manipulating nucleic acids |
| JP2003304899A (en) * | 2002-04-11 | 2003-10-28 | Yoshinobu Baba | Method and apparatus for analyzing nucleic acid |
Non-Patent Citations (1)
| Title |
|---|
| TOJO H. ET AL.: "A rapid methods for screening transgenic mice by PCR with small amounts of blood", EXP. ANIM., vol. 40, no. 2, 1991, pages 235 - 238 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011024947A1 (en) * | 2009-08-31 | 2011-03-03 | 株式会社ハウステック | Molded structure |
| JP2011053334A (en) * | 2009-08-31 | 2011-03-17 | Housetec Inc | Molded structure |
| WO2011040504A1 (en) * | 2009-09-30 | 2011-04-07 | 凸版印刷株式会社 | Nucleic acid analyzer |
| JP2012161340A (en) * | 2009-09-30 | 2012-08-30 | Toppan Printing Co Ltd | Nucleic acid analyzer, and method for isolating and purifying nucleic acid |
| US9267890B2 (en) | 2009-09-30 | 2016-02-23 | Toppan Printing Co., Ltd. | Nucleic acid analyzer |
| JP2012075413A (en) * | 2010-10-05 | 2012-04-19 | Hitachi High-Technologies Corp | Apparatus for inspecting nucleic acid and method of conveying container |
Also Published As
| Publication number | Publication date |
|---|---|
| JPWO2008123019A1 (en) | 2010-07-15 |
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