WO2008119009A4 - Matériaux et procédés pour une production efficace d'alanine - Google Patents
Matériaux et procédés pour une production efficace d'alanine Download PDFInfo
- Publication number
- WO2008119009A4 WO2008119009A4 PCT/US2008/058410 US2008058410W WO2008119009A4 WO 2008119009 A4 WO2008119009 A4 WO 2008119009A4 US 2008058410 W US2008058410 W US 2008058410W WO 2008119009 A4 WO2008119009 A4 WO 2008119009A4
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- genetically modified
- alanine
- brevibacterium
- modified microorganism
- gene
- Prior art date
Links
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 title claims abstract 9
- 238000000034 method Methods 0.000 title claims 11
- 235000004279 alanine Nutrition 0.000 title claims 5
- 244000005700 microbiome Species 0.000 claims abstract 23
- 229960003767 alanine Drugs 0.000 claims abstract 8
- 235000000346 sugar Nutrition 0.000 claims abstract 7
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 claims abstract 4
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims abstract 4
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 claims abstract 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract 3
- 229930006000 Sucrose Natural products 0.000 claims abstract 3
- 239000008103 glucose Substances 0.000 claims abstract 3
- 239000005720 sucrose Substances 0.000 claims abstract 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims abstract 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims abstract 2
- 238000012217 deletion Methods 0.000 claims 8
- 230000037430 deletion Effects 0.000 claims 8
- 108090000623 proteins and genes Proteins 0.000 claims 8
- 230000002779 inactivation Effects 0.000 claims 7
- 108010031025 Alanine Dehydrogenase Proteins 0.000 claims 6
- 241000186146 Brevibacterium Species 0.000 claims 6
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims 5
- 238000003780 insertion Methods 0.000 claims 4
- 230000037431 insertion Effects 0.000 claims 4
- 239000002609 medium Substances 0.000 claims 4
- 241000187561 Rhodococcus erythropolis Species 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims 3
- 239000011707 mineral Substances 0.000 claims 3
- 150000003839 salts Chemical class 0.000 claims 3
- 241000590020 Achromobacter Species 0.000 claims 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 claims 2
- 108010059892 Cellulase Proteins 0.000 claims 2
- 241000186226 Corynebacterium glutamicum Species 0.000 claims 2
- 108010046276 FLP recombinase Proteins 0.000 claims 2
- 102000019259 Succinate Dehydrogenase Human genes 0.000 claims 2
- 108010012901 Succinate Dehydrogenase Proteins 0.000 claims 2
- 230000003115 biocidal effect Effects 0.000 claims 2
- 210000000349 chromosome Anatomy 0.000 claims 2
- 235000003869 genetically modified organism Nutrition 0.000 claims 2
- 230000010354 integration Effects 0.000 claims 2
- 101150117081 51 gene Proteins 0.000 claims 1
- 108010092060 Acetate kinase Proteins 0.000 claims 1
- 108010041525 Alanine racemase Proteins 0.000 claims 1
- 241000588813 Alcaligenes faecalis Species 0.000 claims 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims 1
- 241000186063 Arthrobacter Species 0.000 claims 1
- 241000185996 Arthrobacter citreus Species 0.000 claims 1
- 235000012539 Bacterium linens Nutrition 0.000 claims 1
- 241001430265 Beijerinckia indica subsp. indica Species 0.000 claims 1
- 241000186310 Brevibacterium linens Species 0.000 claims 1
- 108020004705 Codon Proteins 0.000 claims 1
- 241000186216 Corynebacterium Species 0.000 claims 1
- 241001517047 Corynebacterium acetoacidophilum Species 0.000 claims 1
- 241000186145 Corynebacterium ammoniagenes Species 0.000 claims 1
- 241000186248 Corynebacterium callunae Species 0.000 claims 1
- 241000983382 Curtobacterium pusillum Species 0.000 claims 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 claims 1
- 241000588700 Dickeya chrysanthemi Species 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 241001518248 Gluconobacter cerinus Species 0.000 claims 1
- 241000589232 Gluconobacter oxydans Species 0.000 claims 1
- 241000588749 Klebsiella oxytoca Species 0.000 claims 1
- 241000983412 Microbacterium saperdae Species 0.000 claims 1
- 241000203815 Microbacterium testaceum Species 0.000 claims 1
- 108090000854 Oxidoreductases Proteins 0.000 claims 1
- 102000004316 Oxidoreductases Human genes 0.000 claims 1
- 241000185994 Pseudarthrobacter oxydans Species 0.000 claims 1
- 241000319304 [Brevibacterium] flavum Species 0.000 claims 1
- 229940005347 alcaligenes faecalis Drugs 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 101150070629 celY gene Proteins 0.000 claims 1
- 229940106157 cellulase Drugs 0.000 claims 1
- 238000011109 contamination Methods 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 claims 1
- 108010008221 formate C-acetyltransferase Proteins 0.000 claims 1
- 238000012239 gene modification Methods 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 230000005017 genetic modification Effects 0.000 claims 1
- 235000013617 genetically modified food Nutrition 0.000 claims 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 108010083856 methylglyoxal synthase Proteins 0.000 claims 1
- 150000002972 pentoses Chemical class 0.000 claims 1
- -1 Pentose sugars Chemical class 0.000 abstract 4
- 150000008163 sugars Chemical class 0.000 abstract 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 abstract 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 abstract 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 abstract 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 abstract 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 abstract 1
- 239000000470 constituent Substances 0.000 abstract 1
- 150000002016 disaccharides Chemical class 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 239000008101 lactose Substances 0.000 abstract 1
- 239000002029 lignocellulosic biomass Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
- C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La présente invention concerne des micro-organismes génétiquement modifiés qui produisent de la L-alanine comme produit de fermentation primaire à partir de sucres. Des sucres de pentose, tels que le xylose, et des sucres d'hexose, tels que le glucose, peuvent être efficacement fermentés en L-alanine. Les souches décrites ici ont la capacité de métaboliser tous les sucres qui constituent la biomasse lignocellulosique et une variété de disaccharides, y compris le lactose, le maltose, le saccharose et d'autres.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US90823407P | 2007-03-27 | 2007-03-27 | |
| US60/908,234 | 2007-03-27 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2008119009A2 WO2008119009A2 (fr) | 2008-10-02 |
| WO2008119009A3 WO2008119009A3 (fr) | 2009-02-26 |
| WO2008119009A4 true WO2008119009A4 (fr) | 2009-04-16 |
Family
ID=39789270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2008/058410 WO2008119009A2 (fr) | 2007-03-27 | 2008-03-27 | Matériaux et procédés pour une production efficace d'alanine |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2008119009A2 (fr) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003287625A1 (en) | 2002-11-06 | 2004-06-03 | University Of Florida | Materials and methods for the efficient production of acetate and other products |
| CA2688938C (fr) | 2007-03-20 | 2018-01-16 | University Of Florida Research Foundation, Inc. | Matieres et procedes pour une production efficace de succinate et de malate |
| WO2010059616A2 (fr) | 2008-11-18 | 2010-05-27 | University Of Florida Research Foundation, Inc. | Biocatalyseurs et procédés pour la conversion d'hydrolysats d'hémicellulose en produits à base biologique |
| CA2756705C (fr) | 2009-04-02 | 2017-04-11 | University Of Florida Research Foundation, Inc. | Cellules bacteriennes modifiees pour production de scuccinate |
| CN101974476A (zh) * | 2010-08-31 | 2011-02-16 | 安徽华恒生物工程有限公司 | 一种高产l-丙氨酸的xz-a26菌株及构建方法与应用 |
| CN103045528B (zh) * | 2012-12-28 | 2014-05-07 | 安徽华恒生物工程有限公司 | 生产dl-丙氨酸的工程菌及利用该工程菌生产dl-丙氨酸的方法 |
| WO2014100920A1 (fr) * | 2012-12-28 | 2014-07-03 | 安徽华恒生物工程有限公司 | Production de bactéries génétiquement modifiées dl-alanine et son procédé de mise en oeuvre |
| MX2016002651A (es) * | 2013-08-30 | 2016-09-23 | Basf Se | Microorganismo modificado para la produccion mejorada de alanina. |
| MX377149B (es) * | 2013-12-13 | 2025-03-07 | Basf Se | Microorganismo recombinante para la produccion mejorada de productos quimicos finos. |
| JP2017514464A (ja) * | 2014-04-30 | 2017-06-08 | エボニック デグサ ゲーエムベーハーＥｖｏｎｉｋ Ｄｅｇｕｓｓａ ＧｍｂＨ | 好アルカリ菌を使用するｌ−アミノ酸の製造方法 |
| MY186899A (en) | 2015-03-18 | 2021-08-26 | Basf Se | Recombinant microorganism for improved production of fine chemicals |
| CN104774790B (zh) * | 2015-04-03 | 2020-11-06 | 江南大学 | 一种高效发酵生产l-丙氨酸的大肠杆菌 |
| MX389370B (es) * | 2015-06-04 | 2025-03-20 | Basf Se | Microorganismo recombinante para la produccion mejorada de quimicos finos. |
| MY189932A (en) | 2015-06-12 | 2022-03-22 | Basf Se | Recombinant microorganism for improved production of alanine |
| CN110904062B (zh) * | 2018-09-18 | 2022-03-15 | 安徽华恒生物科技股份有限公司 | 一株高产l-丙氨酸的菌株 |
| CN110982857A (zh) * | 2019-09-23 | 2020-04-10 | 安徽丰原生物化学股份有限公司 | 一种l-丙氨酸的发酵生产方法 |
| CN114457122B (zh) * | 2020-05-13 | 2023-06-20 | 安徽华恒生物科技股份有限公司 | 生产l-缬氨酸的重组微生物及构建方法、应用 |
| CN115960942A (zh) * | 2021-10-08 | 2023-04-14 | 安徽丰原生物技术股份有限公司 | 一种生产l-丙氨酸的基因工程菌株及其构建方法和应用 |
| CN115960941A (zh) * | 2021-10-08 | 2023-04-14 | 安徽丰原生物技术股份有限公司 | 一种生产dl-丙氨酸的基因工程菌株及其构建方法和应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6372457B1 (en) * | 1997-01-14 | 2002-04-16 | Arkion Life Sciences Llc | Process and materials for production of glucosamine |
| US7052883B2 (en) * | 2001-04-03 | 2006-05-30 | Degussa Ag | Process for the production of L-amino acids using strains of the family Enterobacteriaceae that contain an attenuated fruR gene |
| US7037504B2 (en) * | 2001-10-23 | 2006-05-02 | Waratah Pharmaceuticals, Inc. | Epidermal growth factor protein and gene, and methods of use therefor |
| DE10210527A1 (de) * | 2002-03-09 | 2003-09-18 | Degussa | Allele des aceA-Gens aus coryneformen Bakterien |
-
2008
- 2008-03-27 WO PCT/US2008/058410 patent/WO2008119009A2/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008119009A2 (fr) | 2008-10-02 |
| WO2008119009A3 (fr) | 2009-02-26 |
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