WO2008039690A3 - Recombinant renalase - Google Patents
Recombinant renalase Download PDFInfo
- Publication number
- WO2008039690A3 WO2008039690A3 PCT/US2007/079047 US2007079047W WO2008039690A3 WO 2008039690 A3 WO2008039690 A3 WO 2008039690A3 US 2007079047 W US2007079047 W US 2007079047W WO 2008039690 A3 WO2008039690 A3 WO 2008039690A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- renalase
- recombinant
- pichia pastoris
- protein
- provides
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
- C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention provides methods of producing recombinant renalase using a methylotrophic yeast such as Pichia pastoris. The invention provides a method for producing recombinant renalase protein by culturing a Pichia pastoris harboring a renalase coding sequence operably linked to a promoter. The recombinant Pichia pastoris is cultured under conditions that permit or induce expression of the renalase protein. The recombinant renalase protein may then be recovered from cell extracts, from culture medium or culture supernatants. Recombinant renalase produced in accordance with the invention is stable, and abundant amounts of renalase can be produced. The present invention also provides for recombinant forms of renalase, including recombinant vectors and cells containing a renalase coding sequence.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US84676006P | 2006-09-25 | 2006-09-25 | |
| US60/846,760 | 2006-09-25 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2008039690A2 WO2008039690A2 (en) | 2008-04-03 |
| WO2008039690A3 true WO2008039690A3 (en) | 2008-10-09 |
Family
ID=39230877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2007/079047 WO2008039690A2 (en) | 2006-09-25 | 2007-09-20 | Recombinant renalase |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2008039690A2 (en) |
-
2007
- 2007-09-20 WO PCT/US2007/079047 patent/WO2008039690A2/en active Application Filing
Non-Patent Citations (2)
| Title |
|---|
| HE ET AL.: "Production of the rat complement regulator, Crry, as an active soluble protein in Pichia pastoris", ARCH BIOCHEM BIOPHYS., vol. 341, no. 2, 15 May 1997 (1997-05-15), pages 347 - 352 * |
| XU ET AL.: "Renalase is a novel, soluble monomine oxidase that regulates cardiac function and blood pressure", J. CLIN. INVEST., vol. 115, no. 5, May 2005 (2005-05-01), pages 1275 - 1280, XP003013119 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008039690A2 (en) | 2008-04-03 |
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