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WO2008035169A3 - Procédé de préparation de cellules hépathiques à partir de cellules progénitrices présentes dans le sang du cordon ombilical - Google Patents

Procédé de préparation de cellules hépathiques à partir de cellules progénitrices présentes dans le sang du cordon ombilical Download PDF

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Publication number
WO2008035169A3
WO2008035169A3 PCT/IB2007/002699 IB2007002699W WO2008035169A3 WO 2008035169 A3 WO2008035169 A3 WO 2008035169A3 IB 2007002699 W IB2007002699 W IB 2007002699W WO 2008035169 A3 WO2008035169 A3 WO 2008035169A3
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WO
WIPO (PCT)
Prior art keywords
umbilical cord
cord blood
vitro
progenitor cells
hepatocytes
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PCT/IB2007/002699
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English (en)
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WO2008035169A2 (fr
Inventor
Annalisa Crema
Cristina Haas
Massimo Sanchez
Antonella Lisi
Antonio Ponzetto
Ercole Brunetti
Rodolfo Marchese
Guido Carloni
Original Assignee
Univ Degli Studi Torino
Consiglio Nazionale Ricerche
Annalisa Crema
Cristina Haas
Massimo Sanchez
Antonella Lisi
Antonio Ponzetto
Ercole Brunetti
Rodolfo Marchese
Guido Carloni
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Univ Degli Studi Torino, Consiglio Nazionale Ricerche, Annalisa Crema, Cristina Haas, Massimo Sanchez, Antonella Lisi, Antonio Ponzetto, Ercole Brunetti, Rodolfo Marchese, Guido Carloni filed Critical Univ Degli Studi Torino
Publication of WO2008035169A2 publication Critical patent/WO2008035169A2/fr
Publication of WO2008035169A3 publication Critical patent/WO2008035169A3/fr

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/06—Anti-neoplasic drugs, anti-retroviral drugs, e.g. azacytidine, cyclophosphamide
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/599—Cell markers; Cell surface determinants with CD designations not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé d'obtention de cultures d'hépatocytes différenciés à partir de cellules progénitrices présentes dans le sang de cordon ombilical de nouveau-nés normaux. En particulier, l'invention concerne un procédé d'obtention d'un système de culture de longue durée qui permet l'expansion et la différenciation in vitro de précurseurs hépatiques purifiés. Des cellules progénitrices pluripotentes sont purifiées par immuno-sélection, par exemple, avec des billes magnétiques et développées in vitro à partir de cellules mononucléaires présentes dans le sang de cordon ombilical. La culture d'hépatocytes obtenue par le système ainsi identifié est appropriée pour une thérapie cellulaire qui remplace des hépatocytes malades par des hépatocytes sains issus de cellules souches homologues. Cette culture d'hépatocytes peut également être utilisée pour le développement d'organes bio-artificiels et, dans un autre mode de réalisation, pour la préparation de systèmes in vitro pour la production in vitro de virus hépatotropes et/ou de leurs composants (à savoir, le virus VHC).
PCT/IB2007/002699 2006-09-18 2007-09-18 Procédé de préparation de cellules hépathiques à partir de cellules progénitrices présentes dans le sang du cordon ombilical WO2008035169A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITMI20061772 ITMI20061772A1 (it) 2006-09-18 2006-09-18 Procedimento per la preparazione di cellule epatiche da cellule progenitrici presenti nel sangue di cordone ombelicale
ITMI2006A001772 2006-09-18

Publications (2)

Publication Number Publication Date
WO2008035169A2 WO2008035169A2 (fr) 2008-03-27
WO2008035169A3 true WO2008035169A3 (fr) 2008-06-12

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PCT/IB2007/002699 WO2008035169A2 (fr) 2006-09-18 2007-09-18 Procédé de préparation de cellules hépathiques à partir de cellules progénitrices présentes dans le sang du cordon ombilical

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IT (1) ITMI20061772A1 (fr)
WO (1) WO2008035169A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013189521A1 (fr) * 2012-06-19 2013-12-27 Waclawczyk Simon Procédé de génération de cellules de phénotype hépatocytaire
CN109370991A (zh) * 2018-11-22 2019-02-22 上海长海医院 一种膀胱癌干细胞标志物及标记方法、膀胱癌分子分型试剂盒及应用
AU2021235252A1 (en) 2020-03-11 2022-10-06 Bit Bio Limited Method of generating hepatic cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004044182A2 (fr) * 2002-11-13 2004-05-27 Washington University Lignees cellulaires hautement permissives pour replication de l'arn du virus de l'hepatite c

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004044182A2 (fr) * 2002-11-13 2004-05-27 Washington University Lignees cellulaires hautement permissives pour replication de l'arn du virus de l'hepatite c

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRASS VOLKER ET AL: "Molecular virology of hepatitis C virus (HCV): 2006 update.", INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 2006, vol. 3, no. 2, 1 April 2006 (2006-04-01), pages 29 - 34, XP002475215, ISSN: 1449-1907 *
HONG S H ET AL: "In vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocyte-like cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 330, no. 4, 20 May 2005 (2005-05-20), pages 1153 - 1161, XP004844850, ISSN: 0006-291X *
ROUNTREE C BART ET AL: "A CD133-expressing murine liver oval cell population with bilineage potential", STEM CELLS (MIAMISBURG), vol. 25, no. 10, 21 June 2007 (2007-06-21), pages 2419 - 2429, XP009098188, ISSN: 1066-5099 *
SHMELKOV S V ST ET AL: "AC133/CD133/Prominin-1", INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY, EXETER, GB, vol. 37, no. 4, April 2005 (2005-04-01), pages 715 - 719, XP004733381, ISSN: 1357-2725 *

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Publication number Publication date
ITMI20061772A1 (it) 2008-03-19
WO2008035169A2 (fr) 2008-03-27

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