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WO2008035169A3 - Process for preparing hepatic cells from progenitor cells present in umbilical cord blood - Google Patents

Process for preparing hepatic cells from progenitor cells present in umbilical cord blood Download PDF

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Publication number
WO2008035169A3
WO2008035169A3 PCT/IB2007/002699 IB2007002699W WO2008035169A3 WO 2008035169 A3 WO2008035169 A3 WO 2008035169A3 IB 2007002699 W IB2007002699 W IB 2007002699W WO 2008035169 A3 WO2008035169 A3 WO 2008035169A3
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WO
WIPO (PCT)
Prior art keywords
umbilical cord
cord blood
vitro
progenitor cells
hepatocytes
Prior art date
Application number
PCT/IB2007/002699
Other languages
French (fr)
Other versions
WO2008035169A2 (en
Inventor
Annalisa Crema
Cristina Haas
Massimo Sanchez
Antonella Lisi
Antonio Ponzetto
Ercole Brunetti
Rodolfo Marchese
Guido Carloni
Original Assignee
Univ Degli Studi Torino
Consiglio Nazionale Ricerche
Annalisa Crema
Cristina Haas
Massimo Sanchez
Antonella Lisi
Antonio Ponzetto
Ercole Brunetti
Rodolfo Marchese
Guido Carloni
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Degli Studi Torino, Consiglio Nazionale Ricerche, Annalisa Crema, Cristina Haas, Massimo Sanchez, Antonella Lisi, Antonio Ponzetto, Ercole Brunetti, Rodolfo Marchese, Guido Carloni filed Critical Univ Degli Studi Torino
Publication of WO2008035169A2 publication Critical patent/WO2008035169A2/en
Publication of WO2008035169A3 publication Critical patent/WO2008035169A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/06—Anti-neoplasic drugs, anti-retroviral drugs, e.g. azacytidine, cyclophosphamide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/599—Cell markers; Cell surface determinants with CD designations not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention concerns a process for obtaining cultures of differentiated hepatocytes starting from progenitor cells present in the umbilical cord blood of normal newborns. In particular the invention relates to a process for obtaining a long-term culturing system which allows the in vitro expansion and differentiation of purified hepatic precursors. Pluripotent progenitor cells are purified by immuno-selection, for example with magnetic beads and expanded in vitro starting from mononuclear cells present in umbilical cord blood. The hepatocyte culture obtained with the thus identified system is suitable for cell therapy which replaces diseased hepatocytes with healthy hepatocytes deriving from homologous stem cells. This hepatocyte culture can also be used for the development of bioartificial organs and, in a further embodiment, for the preparation of in vitro systems for the in vitro production of hepatotropic viruses and/or of their components (i.e. the HCV virus).
PCT/IB2007/002699 2006-09-18 2007-09-18 Process for preparing hepatic cells from progenitor cells present in umbilical cord blood WO2008035169A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITMI20061772 ITMI20061772A1 (en) 2006-09-18 2006-09-18 PROCEDURE FOR THE PREPARATION OF HEPATIC CELLS FROM PROGENITRIC CELLS PRESENT IN THE BLOOD OF CORDLESS CORD
ITMI2006A001772 2006-09-18

Publications (2)

Publication Number Publication Date
WO2008035169A2 WO2008035169A2 (en) 2008-03-27
WO2008035169A3 true WO2008035169A3 (en) 2008-06-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2007/002699 WO2008035169A2 (en) 2006-09-18 2007-09-18 Process for preparing hepatic cells from progenitor cells present in umbilical cord blood

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IT (1) ITMI20061772A1 (en)
WO (1) WO2008035169A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013189521A1 (en) * 2012-06-19 2013-12-27 Waclawczyk Simon Method of generating cells of hepatocyte phenotype
CN109370991A (en) * 2018-11-22 2019-02-22 上海长海医院 Bladder cancer stem cell marker and labeling method, bladder cancer molecular typing kit and application
AU2021235252A1 (en) 2020-03-11 2022-10-06 Bit Bio Limited Method of generating hepatic cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004044182A2 (en) * 2002-11-13 2004-05-27 Washington University Highly permissive cell lines for hepatitis c virus replication

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004044182A2 (en) * 2002-11-13 2004-05-27 Washington University Highly permissive cell lines for hepatitis c virus replication

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRASS VOLKER ET AL: "Molecular virology of hepatitis C virus (HCV): 2006 update.", INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 2006, vol. 3, no. 2, 1 April 2006 (2006-04-01), pages 29 - 34, XP002475215, ISSN: 1449-1907 *
HONG S H ET AL: "In vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocyte-like cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 330, no. 4, 20 May 2005 (2005-05-20), pages 1153 - 1161, XP004844850, ISSN: 0006-291X *
ROUNTREE C BART ET AL: "A CD133-expressing murine liver oval cell population with bilineage potential", STEM CELLS (MIAMISBURG), vol. 25, no. 10, 21 June 2007 (2007-06-21), pages 2419 - 2429, XP009098188, ISSN: 1066-5099 *
SHMELKOV S V ST ET AL: "AC133/CD133/Prominin-1", INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY, EXETER, GB, vol. 37, no. 4, April 2005 (2005-04-01), pages 715 - 719, XP004733381, ISSN: 1357-2725 *

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Publication number Publication date
ITMI20061772A1 (en) 2008-03-19
WO2008035169A2 (en) 2008-03-27

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