WO2008018133A1

WO2008018133A1 - Moisturizing agent, cell-activating agent, skin-whitening agent, agent for suppressing triglyceride accumulation, antioxidant and external preparation for skin

Info

Publication number: WO2008018133A1
Application number: PCT/JP2006/315808
Authority: WO
Inventors: Nono Yamamura; Masaki Arashima; Yumi Mimasu
Original assignee: Noevir Co., Ltd.
Priority date: 2006-08-10
Filing date: 2006-08-10
Publication date: 2008-02-14
Also published as: JPWO2008018133A1

Abstract

The invention relates to a moisturizing agent, a cell-activating agent, a skin-whitening agent, an agent for suppressing triglyceride accumulation and an antioxidant containing as an active ingredient, one or more plants selected from plants belonging to the Osmundaceae family or an extract therefrom. Further, an external preparation for skin containing one or more plants selected from plants belonging to the Osmundaceae family or an extract therefrom is disclosed.

Description

Specification

Moisturizer, cell activator, whitening agent, neutral fat accumulation inhibitor, antioxidant, and topical skin preparation

Technical field

The present invention relates to a humectant, a cell activator, a whitening agent, a neutral fat accumulation inhibitor, an antioxidant, and an external preparation for skin, which comprise a naturally derived component as an active ingredient. For more information, click here

Osmundaceae) The present invention relates to a moisturizer, a cell activator, a whitening agent, a neutral fat accumulation inhibitor, an antioxidant, and a skin external preparation containing a plant extract.

Background art

[0002] As factors of dermatoses such as aging, disease, stress, UV blemishes, blemishes, reduced skin elasticity, dryness, decreased cellular function, melanin production and pigmentation due to UV rays, decrease in dermal matrix components And cell oxidative damage due to degeneration, ultraviolet rays and the like. In order to prevent and improve such skin symptoms, various active ingredients have been searched and compounded. In particular, naturally derived ingredients are known to have various pharmacological and cosmetic effects, and so far the application of extracts such as plants and fungi to skin preparations has been studied.

[0003] For example, orchidaceae plants (see JP 2002-205933), agar extract (see JP 2002-193820), peanut seed coat extract (JP 2002-14575 7) as moisturizers. No. 2), psyllium extract (see JP 2002-145756 A), longan seed extract (see JP 2002-145732 A), and leafhopper extract (see JP 2002-14573 1). Are known. The essence of bonkan as cell activator (see JP 2001131045), water and Z or organic solvent extract of white crane reishi as whitening agent (see JP 2003-89630), neutral fat accumulation inhibitor An extract of a plant belonging to the genus Shippogoke (see JP-A-2004-331580) and an extract of a plant belonging to the genus Salogase family (see JP-A-10-182413) are known as antioxidants.

[0004] Thus, various naturally-derived components have been applied so far. However, there are many naturally-derived components whose effects are not yet known. The development of an active ingredient having a cell activation effect, a whitening effect, a neutral fat accumulation inhibitory effect, an antioxidant effect and the like was expected.

Disclosure of the invention

[0005] As a result of studying various components derived from nature, the inventors of the present invention have excellent moisturizing action, cell activation action, whitening, and the like, which are superior to the oyster family plant extract whose effects have not been known. It has been found that there are an action, a neutral fat accumulation inhibitory action, and an antioxidant action, and further studies have been made to complete the present invention.

[0006] That is, the present invention relates to a moisturizer, cell activator, whitening agent, neutral fat accumulation inhibitor, comprising one or more plants selected from genus genus or an extract thereof as an active ingredient, Or relates to antioxidants.

Another aspect of the present invention relates to a skin external preparation characterized by containing one or two or more kinds of plants selected from the genus genus plants or extracts thereof.

[0007] According to the present invention, a moisturizer, cell activator, whitening agent having an excellent effect can be obtained by using one or two or more plants selected from the genus genus plant or an extract thereof as an active ingredient. Agents, neutral fat accumulation inhibitors, and antioxidants can be provided.

When these moisturizers, cell activators, whitening agents, neutral fat accumulation inhibitors, and antioxidants are added to skin external preparations such as cosmetics and external medicines, wrinkles, tarmi, reduced skin elasticity, It is possible to provide various compositions that exhibit excellent effects in preventing and improving the appearance of various skin symptoms such as dullness.

BEST MODE FOR CARRYING OUT THE INVENTION

[0008] The Osmundaceae plant is a primitive fern plant, and the genus Osm unda, Todea, and Lentonteris are known. Osmunda plants include: Osmunda iaponica, Osmunda reealis, Osmunda lancea, Osmunda intermedia, Osmunda cinnamomea, Omunda cinnamomea, mundo clavtoniana) and Osmunda banksiifolia are known.

These plants can be used alone or in combination of two or more. it can.

[0009] In the present invention, it is not particularly limited as long as it is a member of the genus genus, but a mainspring (Osmunda iaponica) can be raised as a suitable one because it is relatively easy to obtain. [0010] When a plant is used, any part such as a spore body or gametophyte root, stem, trunk, leaf, or young shoot, which is not particularly limited, can be used. Use multiple parts in combination.

They can be crushed as they are, but it is preferable to use extracts that are strong in their parts!

[0011] For extraction, any part of the spirophytes or gametophytes of the oyster family can be used. For easy use, the whole plant, root, leaf blade, petiole, etc. of the spore body can be used. Good. At that time, an extract may be obtained using a plurality of parts. You can also use a mixture of two or more extracts extracted using different solvents.

In the extraction, the plant may be used as it is. However, considering the extraction efficiency, it is preferable to perform the extraction after processing such as shredding, drying, and powdering.

The extraction can be performed by immersing in an arbitrary extraction solvent for a predetermined time. The extraction solvent may be heated as necessary. Alternatively, extraction can be performed using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be stirred or homogenized in an extraction solvent. The extraction temperature is suitably about 5 ° C to the boiling point of the extraction solvent. Although the extraction time varies depending on the type of extraction solvent and the extraction temperature, it is appropriate that the extraction time be about 1 hour to 14 days.

[0012] Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerine; ethyl ether, propyl ether Solvents such as ethers such as butyl acetate, butyl acetate, ethyl acetate and the like; ketones such as acetone, ethylmethyl ketone and the like can be used. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline and the like may be used. In addition, one or more supercritical liquids such as water, carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia, etc. A field liquid may be used.

[0013] The extract of the oyster family plant using the above-mentioned solvent may be used as it is. It may be left as it is for a certain period of time to be aged, or the concentrated and dried product may be used again as water or a polar solvent. It can also be used by dissolving. Alternatively, it may be used after performing purification treatment such as decolorization, deodorization and desalting, and fractionation treatment by column chromatography or the like within the range not impairing these physiological functions. The above-mentioned extracts of the oyster family plants and their treated products and fractions can be freeze-dried after each treatment and fractionation and dissolved in a solvent at the time of use. Moreover, it can also be used by being encapsulated in vesicles such as ribosomes, microcapsules and the like.

[0014] The genus plant or extract thereof has an excellent moisturizing action, cell activation action, whitening action, neutral fat accumulation inhibiting action, antioxidant action, moisturizing agent, cell activator, whitening agent, neutrality It can be used as a fat accumulation inhibitor and an antioxidant.

Each of these agents is not limited at all in terms of its form and the presence or absence of other components as long as it contains a genus plant or its extract as an active ingredient. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use etc., and the vehicle (excipient), solvent, Other general additives (anti-oxidation agent, colorant, dispersant, etc.) can optionally be included.

[0015] A moisturizing agent containing a spring family plant or an extract thereof as an active ingredient provides an excellent moisturizing effect on the skin and hair, etc., and also has an excellent effect on improving rough skin, fine lines, dullness and skin symptoms. Demonstrate, improve skin texture and enhance skin transparency.

[0016] A cell activator comprising a spring family plant or an extract thereof as an active ingredient has an excellent dermal fibroblast activation effect and an epidermal cell activation effect, and exhibits an excellent cell activation effect.

[0017] A whitening agent comprising an Amaranthaceae plant or an extract thereof as an active ingredient has an excellent melanin production inhibitory effect and tyrosinase activity inhibitory effect, and is excellent in preventing and improving pigmentation, stains, freckles, etc. Demonstrate whitening effect.

[0018] A neutral fat accumulation inhibitor comprising a spring plant or an extract thereof as an active ingredient has an excellent neutral fat accumulation inhibitory effect and exhibits an excellent neutral fat accumulation inhibitory effect.

[0019] An antioxidant containing an apricot family plant or an extract thereof as an active ingredient is an excellent radical. It has an excellent anti-oxidant effect and has an erasing effect of superoxide-on and superoxide-on.

[0020] A variety of skins such as wrinkles, tarmi, stains, dullness, dryness, fine wrinkles, etc. are obtained by blending the spring power plant or its extract into a skin external preparation such as cosmetics, topical pharmaceuticals, and quasi drugs. Prevention of symptoms · An external preparation for skin that exhibits an excellent improvement effect can be obtained. Therefore, it can be preferably used as, for example, a moisturizing skin external preparation or a whitening skin external preparation.

[0021] The amount of the spring power plant or the extract thereof in the external preparation for skin can be adjusted according to the type and purpose of the external preparation for skin, but the total amount of the external preparation for skin from the viewpoint of the effect and stability. In terms of solid content, 0.0001 to 10.0% by mass is more preferable, 0.001 to 5.0% by mass, still more preferably 0.01 to 5% by mass. , rather more preferably is 0.1 to 5 mass ^_0/0.

[0022] The dosage form of the external preparation for skin is arbitrary, and for example, various dosage forms such as lotion, emulsion, gel, cream, ointment, powder, granule, pack, patch, patch, aerosol, etc. Can be provided.

[0023] In addition to the genus plant or extract thereof, the topical skin preparation is usually used for pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics and cleansing agents, etc., depending on the use and necessity. Optional ingredients to be formulated, such as water, oily ingredients, humectants, powders, pigments, emulsifiers, solubilizers, gelling agents, cleaning agents, UV absorbers, anti-inflammatory agents, thickeners, pH adjusters, Surfactants, chelating agents, drugs (medicinal ingredients), fragrances, rosin, antibacterial and antifungal agents, antioxidants, alcohols and the like can be appropriately blended. Furthermore, within the range not impairing the effects of the present invention, other moisturizers, cell activators, whitening agents, neutral fat accumulation inhibitors, antioxidants, or combinations of plants other than the genus family or extracts thereof Is also possible.

Example

[0024] Hereinafter, preparation examples of spring plant extracts, tests for evaluating each action, formulation examples as a topical skin preparation, and use tests will be described in more detail, but the technical scope of the present invention is described below. It is not limited at all.

<Preparation method 1> 20 liters of 50% by weight ethanol aqueous solution was added to 1 kg of dried pulverized whole plant spores of Osmunda iaponica and soaked at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, 30 g of the spring plant extract was obtained.

Water (20 liters) was added to 1 kg of dry ground pulverized whole body of spring main body, and extracted by refluxing at 90 ° C for 6 hours. The extract was collected by filtration, and after removing the solvent, 20 g of an extract of the genus genus plant was obtained.

20 kg of methanol was added to 1 kg of dried pulverized whole body of spring main body and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, a spring extract 5 Og was obtained.

100 g of dry pulverized whole body of a spring spring sprout was put into a supercritical extraction device, and extracted using a supercritical fluid of carbon dioxide and carbon dioxide at 40 ° C under an atmospheric pressure of 15 MPa. The extract was recovered to obtain 10 g of a spring family plant extract.

[0029] <Evaluation of cell activation 1 (dermal fibroblast activation)>

Evaluation of the dermal fibroblast activation action of the springfish plant extract was performed by the following method. As a sample, a genus plant extract produced by Preparation Method 1 was used. Normal human dermal fibroblasts manufactured by Kurabo Industries Co., Ltd. (Kurashiki Boseki Co., Ltd.) were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% by weight urchin fetal serum (FBS). After culturing for 24 hours, the medium was replaced with 1% by mass FBS-added DMEM medium to which the sample (extract) was added so as to have each concentration shown in Table 1, and further cultured for 48 hours. After removing the supernatant, replace with medium containing 400 gZmL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) and incubate for about 2 hours did. Thereafter, the formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. In the evaluation, the medium containing the sample In addition to the 1 mass ⁰ / ^oFBS添Ka卩DMEM medium as a negative control, using 5 mass ⁰ / ^oFBS添Ka卩DMEM medium as a positive control.

The evaluation results are shown in Table 1 as relative values with the cell activation effect in the negative control as 100.

[table 1]

[0031] As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the spring plant extract. From this, it has been clarified that the spring-like plant extract has an excellent dermal fibroblast activation effect.

[0032] <Evaluation of cell activation 2 (epidermal cell activation)>

Evaluation of the epidermis cell activation effect of the springfish plant extract was performed by the method shown below. As a sample, a genus plant extract produced by Preparation Method 2 was used.

Human epidermal non-keratinized cells were seeded in 96-well microplates at 2.0 × 10 ⁴ cells per well. As a seeding medium, commercially available Humedia-KG2 manufactured by Kurabo Industries, Ltd. was used. After culturing for 24 hours, the medium was replaced with the test medium to which the sample was added at the concentrations shown in Table 2, and further cultured for 24 hours. Next, the medium was replaced with a medium containing 100 μgZmL of 3- (4,5 dimethyl-2 thiazolyl) 2,5 diphenyltetrazolium bromide (MTT) and cultured for 2 hours. -Extracted with propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.

The evaluation results show the cell activation effect in the control using the medium without sample. Table 2 shows the relative values when 0 is assumed.

[0033] [Table 2]

[0034] As is clear from Table 2, a significant epidermal cell activation effect was observed in the medium supplemented with the oyster family plant extract. From this, it has been proved that the plant spring plant extract has an excellent epidermal cell activation effect.

Based on the above, it has been clarified that the oyster plant extract has an excellent cell activation effect.

[0035] <Evaluation of whitening action 1 (melanin production inhibitory action)>

Evaluation of the melanin production inhibitory effect of the springfish plant extract was performed by the following method. As a sample, a genus plant extract produced by Preparation Method 3 was used.

B16 mouse melanoma cells (B16F0 cells) were seeded at a density of 1.8 × 10 ⁴ per dish. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight urine fetal serum (FBS). After 24 hours, the medium was replaced with a sample-added medium adjusted to each concentration shown in Table 4 using a DMEM medium supplemented with 5% by mass FBS, and further cultured for 5 days. After completion of the culture, the cells were detached with trypsin, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. The color of the obtained precipitate was visually judged based on the judgment table shown in Table 3. The evaluation is based on a five-point scale. DMEM medium containing 5% FBS without sample added to the negative control (judgment 5) and 50 mM sodium lactate contained in the positive control (judgment 1) DMEM containing 5 mass ⁰ / oFBS Medium was used.

In order to evaluate the amount of melanin produced, add Soluen-350 (Perkin Elma Japan Co., Ltd.) to the precipitate and boil it, then use a spectrophotometer (Spectrophotometer U-3010 manufactured by Hitachi High-Technologies Corporation). Absorbance at 500 nm was measured.

The results obtained are shown in Table 4. [0036] [Table 3]

[0037] [Table 4]

[0038] As is clear from Table 4, in the medium supplemented with the oyster family plant extract, a significant melanogenesis inhibitory effect was observed. From this, it has been proved that the plant spring plant extract has an excellent inhibitory effect on melanin production.

[0039] <Evaluation of whitening action 2 (Tyrosinase activity inhibitory action)>

Evaluation of the tyrosinase activity inhibitory action of the oyster family plant extracts was carried out by the following method. As a sample, normal human epidermal melanocytes manufactured by Kurabo Industries using the genus genus plant produced by Preparation Method 1 were seeded in 9 6-well microphone mouthplates at 3.0 x 10 ⁴ per well. . As the seeding medium, Mediuml 54S manufactured by Kurabo Industries was used. After 24 hours, the sample-added caro culture medium adjusted to each concentration shown in Table 5 was exchanged with Medium 54S, and cultured for 48 hours. Next, the cells were completely lysed by exchanging with 75 L of 1% by mass Triton-X-containing phosphate buffer, and 50 / zL of the cell was used as a crude enzyme solution. In the crude enzyme solution, 0.05 mass% L-dopa-containing phosphate buffer solution 50 / zL as a substrate was added and allowed to stand at 37 ° C. for 2 hours. Microplate reader 4 immediately after substrate addition and at the end of the reaction Absorbance at 05 nm was measured, and the amount of dopamelanin produced was determined by introducing each measured value into Equation (1).

Formula (1): Amount of produced dopamelanin = {(After reaction 405 nm value-Before reaction 405 nm value "— 2. 166/5. 238

The amount of protein in each well was measured using BIER Protein Assay Kit manufactured by PIERCE, and the amount of dopamelanin produced per unit protein was determined. The measurement results are shown in Table 5 according to the inhibition rate based on the value of the control using the culture medium without sample.

[0040] [Table 5]

[0041] As is clear from Table 5, when the medium supplemented with the oyster family plant extract was used, a decrease in melanin production was observed. From this fact, it has been proved that the oyster family plant extract has an excellent tyrosinase activity inhibitory action.

From the above, it has been clarified that the spring-like plant extract has an excellent whitening effect.

[0042] <Evaluation of neutral fat accumulation inhibitory effect>

Evaluation of the neutral fat accumulation-inhibiting action of the apricot family plant extract in adipocytes was performed by the following method. As a sample, a genus genus plant extract produced by Preparation Method 1 was used.

Subcutaneous fat-derived normal human preadipocytes Cryo · HPRAD SQ (Sanko Junyaku Co., Ltd.) were seeded on a 96-well microphone mouthplate so that there were 1.0 × 10 ⁴ cells per well. As the seeding medium, PGM medium (10% by weight urinary fetal serum (FBS), 2 mM L Glutamine, 1 OOunits / mL Penicilline, 100 μg Z mL Streptomycine¾′¾) was used. Immediately before Ito's saturation is reached, PGM-diluted medium supplemented with the sample concentrations shown in Table 6 (10 μg / mL insulin, 1 M Dexamethasone, 200 μΜ Indomethacin, 50 0 M Isobutyl—containing methylxanthine) to induce differentiation into adipocytes. After initiation of differentiation induction, the cells were cultured for 10 to 14 days until the control group matured and many lipid droplets accumulated in the cells. After the cells were collected, the cells were fixed using 10% neutral buffered formaldehyde solution. After washing with PBS, 0.5 wZv% Oil Red O solution was added and incubated at 37 ° C for 2 hours. After washing with PBS, methanol was added to extract the pigment. After extraction, the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the amount of triglyceride accumulated was measured using the difference between the two measurements.

The measurement results are shown in Table 6 in terms of relative values with the neutral fat accumulation amount set to 100 in the control using the medium without the sample.

[0043] [Table 6]

[0044] As is clear from Table 6, a significant neutral fat accumulation inhibitory effect was observed in the culture medium supplemented with the oyster family plant extract. From this, it has been clarified that the spring-like plant extract has an excellent neutral fat accumulation inhibitory action.

[0045] <Evaluation of antioxidant action 1 (radical scavenging action)>

Evaluation of the antioxidant effect of the springfish plant extract was performed by the following method. As a sample, a genus plant extract produced by Preparation Method 1 was used.

The concentration of each sample was adjusted with 50% by mass ethanol to obtain a sample solution, and 100 / zL was added to a 96-well microplate so that the concentrations shown in Table 7 were obtained. Thereto, 0.2 mM of 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution was added in an amount of 100 μL, mixed well, and allowed to stand at room temperature in the dark for 24 hours. Thereafter, the absorbance at 516 nm derived from the DPPH radical was measured. The extinction rate of DPPH radicals is defined as (A) where the absorbance of the control when the sample is added and when the force is applied is (B) and the absorbance when the sample is added is (B). Introduced into equation (2). The results are shown in Table 7.

Formula (2): Radical scavenging rate = {1 (B) Z (A)} X 100 (%)

[Table 7]

[0047] As is clear from Table 7, an excellent DPPH radical scavenging effect was observed in the oyster family plant extract. From this, it has been proved that the genus plant extract has an excellent radical scavenging action.

[0048] Evaluation of anti-acidic action 2 (superoxide anion scavenging action)>

Evaluation of the superoxide anion scavenging action of the springfish plant extract was carried out by the method described below. As a sample, a genus plant extract produced by Preparation Method 1 was used.

In a 96-well microplate, add Hanks (+) solution 25 / z L of Zeny extract diluted to any concentration as shown in Table 8, and 75 reaction solution (0.25 mM NBT, ImM Hypoxanthine, 0. Hanks (+) solution (pH7.4) containing ImM EDTA was added, xanthine oxidase solution 25 / z I ^ O.0075Units) was added, and the mixture was incubated at 37 ° C for 15 minutes. Thereafter, the absorbance at 540 nm was measured with a microplate reader. When the absorbance of the control with no sample added was (A) and the absorbance when the sample was added was (B), the value of equation (3) was taken as the radical scavenging rate. The evaluation results are shown in Table 8.

Formula (3): {1-(B) / (A)} X 100 (%)

[0049] [Table 8] Concentration (mg / mL) Erase rate (%)

Control mouth 0

0.013 35.2

0.05 74.5

0.20 96.7

[0050] As can be seen from Table 8, the superior oxidization effect of superoxide ions was observed in the genus plantaceae extracts. From this, it was proved that the spring-like plant extract has an excellent superoxide-one erasing action.

From the above, it has been clarified that the oyster family plant extract has an excellent antioxidant action.

[0051] Next, prescription examples of the external preparation for skin containing the extract of the spring family plant obtained by each of the above preparation methods will be shown.

[Example 1] Emulsion

Formula:

[Table A]

(1) Screen 10.0 (mass%)

(2) Methylphenylpolysiloxane 4.0

(3) Hydrogenated palm kernel oil 0.5

(4) Hydrogenated soybean phospholipid 0.1

(5) Polyoxyethylene sorbitan monostearate (20 E. O.)

13

(6) Sorbitan monostearate 1.0 (7) Glycerin 4.0

(8) Methyl paraoxybenzoate 0.1

(9) Carboxyvinyl polymer 0.15

(10) The balance of purified water 1 0 0

(11) Arginine (1 wt% aqueous solution) 2 0. 0

(12) Windmill plant extract (Preparation method 1) 3.0 Production method: Heat-dissolve the oil phase components of (1) to (6) at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) in order to make uniform To mix.

[Example 2] Lotion

Formula:

[Table B]

(1) Ethanol 15.0 (mass%)

(2) Polyoxyethylene (40 E. O.) Hardened castor oil 0.3

(3) Fragrance 0. 1

(4) The balance of purified water 1 0 0

(5) Chenic acid 0.02

(6) Sodium quenate 0.1

(7) Glycerin 1.0

(8) Hydroxyethyl cellulose 0.1

(9) Windmill plant extract (Preparation method 3) 5.0 Production method: Dissolve (2) and (3) in (1). After dissolution, add (4) to (8) sequentially, then stir well, add (9), and mix uniformly.

[Example 3] Cream

Formula:

[Table C]

(I) Scullane 1 0.0 (mass%) (2) Stearic acid 2.0

(3) Hydrogenated palm kernel oil 0.5

(4) Hydrogenated soybean phospholipid 0.1

(5) Seyu Nord 3.6

(6) Lipophilic glyceryl monostearate2.0

(7) Glycerin 1 0. 0

(8) Methyl paraoxybenzoate 0.1

(9) Arginine (20 mass% aqueous solution) 1 5. 0

(10) The balance of purified water 1 0 0

(II) Carboxyvinyl polymer (1% by weight aqueous solution) 1 5. 0

(12) Windmill plant extract (Preparation method 1) 5.0 Production method: Heat-dissolve the oil phase components of (1) to (6) at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification, add (11) and start cooling at 40 ° C (12) And mix evenly.

[Example 4] Cosmetic liquid

Formula:

[Table D]

(1) The balance (mass%) of purified water 1 0 0

(2) Glycerin 0

(3) Sucrose fatty acid ester 3

(4) Carboxypropyl polymer (1 mass% aqueous solution) 5

(5) Sodium alginate (1% by weight aqueous solution) 0

(6) Polyglyceryl monolaurate 0

(7) Macadamia nut oil fatty acid phytosteryl 0

(8) N-lauroyl-L-glutamate di (phytosteryl-2 1 year old cutyldecyl)

2 0

(9) Hardened palm oil 2 0

(10) Sukuran (Olive) 1 0

(11) Behenyl alcohol 0

(12) Beeswax 0

(13) Jojoba oil 1 0

(14) 1, 3-Butylene glycol 0 0

(15) L-Arginine (10% by mass aqueous solution) 2 0

(16) Windmill plant extract (Preparation method 2) 50 Manufacturing method: Mix the aqueous phase components (1) to (6) and dissolve at 75 ° C with heating. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Start cooling after emulsification and cover (15) at 50 ° C. Cool to 40 ° C, add (16), and mix evenly.

[Example 5] Aqueous gel

Formula:

[Table E] (1) Forced L-Poxyvinyl Polymer 0.5 (mass%)

(2) The balance of 100 purified water

(3) Sodium hydroxide (10 mass% aqueous solution) 0.5

(4) Ethanol 1 0. 0

(5) Methyl paraoxybenzoate 0.1

(6) Fragrance 0. 1

(7) Windmill plant extract (Preparation method 2) 3.0

(8) Polyoxyethylene (60 E. O.) hydrogenated castor oil 1.0 Manufacturing method: Add (1) to (2), stir uniformly, and then add (3). After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, add (6) to (8) previously mixed and stir and mix uniformly.

[Example 6] Cleansing fee

Formula:

[Table F]

(1) Screen 8 1 0 (mass%)

(2) Isostearic acid: 1 5. 0

(3) The balance of 100 purified water

(4) Windmill plant extract (Preparation method 3) 4.0 Production method: Dissolve (1) and (2) uniformly. Add (3) and (4) to this, and mix evenly.

[Example 7] Face washing foam

Formula:

[Table G]

(1) Stearic acid 16.0 (mass%)

(2) Myristic acid 1 6. 0

(3) Lipophilic glyceryl monostearate2.0

(4) Glycerin 2 5. 0

(5) Sodium hydroxide 7.5

(6) Palm oil fatty acid amidpropyl betaine 1.0

(7) The balance of 100 purified water

(8) Windmill plant extract (Preparation method 3) 5.0 Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C, and the oil phase components are mixed and stirred uniformly. Start cooling at 40 ° C Add (8) and mix evenly.

[Example 8] Make-up base "

Formula:

[Table H]

(I) squalene 1 0.2 (mass%) (2) cetanol 2.0

(3) Glycerol tree 2-ethylhexanoate 2.5

(4) Lipophilic glyceryl monostearate1.0

(5) Propylene glycol 1 1. 0

(6) Sucrose fatty acid ester 1.3

(7) The balance of 100 purified water

(8) Titanium oxide 1.0

(9) Bengala 0.1

(10) Yellow iron oxide 0.4

(II) Fragrance 0. 1

(12) Windmill plant extract (Preparation method 2) 3.0 Manufacturing method: Mix the oil phase components of (1) to (4), and dissolve by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C. The pigments (8) to (10) are added to this and uniformly dispersed with a homomixer. Let The oil phase component is added to the water phase component and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C and mix evenly.

[Example 9] Emulsion foundation

Formula:

[Table I]

(I) Methylpolysiloxane 2.0 (mass%) (2) Scullane 5.0

(3) Octyldodecyl myristate 5.0

(4) Seyu Nord 1. 0

(5) Polyoxyethylene (20 E. 〇.) Sorbitan monostearate

13

(6) Sorbitan monostearate 0.7

(7) 1,3-Butylene glycol 8.0

(8) Xanthan gum 0.1

(9) Methyl paraoxybenzoate 0.1

(10) The balance of purified water 1 0 0

(II) Titanium oxide 9.0

(12) Talc 7.4

(13) Bengala 0.5

(14) Yellow iron oxide 1.1

(15) Black iron oxide 0.1

(16) Fragrance 0. 1

(17) Windmill plant extract (Preparation method 1) 4.0 Production method: Mix the oil phase components (1) to (6) and dissolve at 75 ° C with heating. On the other hand, the water phase components (7) to (10) are mixed and dissolved by heating at 75 ° C. The pigments (11) to (15) are added to this and uniformly dispersed with a homomixer. To do. Add oil phase ingredients and emulsify. Cooling is started after emulsification, and components (16) and (17) are sequentially added at 40 ° C and mixed uniformly.

[Example 10] Water-in-oil emollient cream

Formula:

[Table J]

(1) Liquid paraffin 34.0 (mass%)

(2) Microcrystalline wax 2.0

(3) Vaseline 5.0

(4) Diglycerinoleate 5.0

(5) Sodium chloride 3

(6) Potassium chloride 0.1

(7) Propylene Daricol 3.0

(8) 1, 3-butylene glycol 5.0

(9) Methyl paraoxybenzoate 0.

(10) Windmill plant extract (Preparation method 1) 3.0

(11) The balance of 100 purified water

(12) Fragrance 0. 1 Manufacturing method: Dissolve (5) and (6) in a part of (11) to 50 ° C, and heat to 50 ° C (4). After mixing this, disperse uniformly into (1) to (3), which is heated and dissolved at 70 ° C. Add (7) to (10) to the remainder of (11) by heating and dissolving at 70 ° C while stirring and emulsify with a homomixer. Start cooling after emulsification, add (12) at 40 ° C, and mix uniformly.

[0061] [Example 11] Pack

Formula:

[Table K]

(1) The balance (mass%) of purified water 100

(2) Polyvinyl alcohol 12.0

(3) Ethanol 1 7.0

(4) Glycerin 9.0

(5) Polyethylene glycol (average molecular weight 1 000) 2.0

(6) Windmill plant extract (Preparation method 2) 5.0

(7) Fragrance 0.1 Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and start cooling with stirring. Cool to 40 ° C, add (6) and (7), and mix evenly.

[Example 12] Bath salt

Formula:

[Representation]

(1) Fragrance 0.3 (mass%)

(2) Windmill plant extract (Preparation method 3) 3.0

(3) Sodium hydrogen carbonate 5 0. 0

(4) Sodium sulfate 46.7 Production method: Mix (1) to (4) uniformly.

[0063] [Example 13] Hair wax

Formula:

[Table M] (1) Stearic acid 3.0 (mass%)

(2) Microcrystalline wax 2.0

(3) Cetyl alcohol 3.0

(4) Highly polymerized methylpolysiloxane 2.0

(5) Methylpolysiloxane 5.0

(6) Poly (oxyethylene / oxypropylene) methylpolysiloxane copolymer

Ten

(7) Methyl paraoxybenzoate 0.1

(8) 1, 3-butylene glycol 7-5

(9) Arginine 0.7

(10) The balance of 100 purified water

(11) Windmill plant extract (Preparation method 3) 4.0

(12) Fragrance 0.1 Production method: Mix the oil phase components (1) to (6), and heat and dissolve at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C, and the oil phase component is added and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C, and mix uniformly.

[0064] [Example 14] Hair art nick

Formula:

[Table N]

(1) Ethanol 50.0 (mass%)

(2) The balance of 100 purified water

(3) Windmill plant extract (Preparation method 1) 3.0

(4) Fragrance 0.1 Production method: Mix and homogenize components (1) to (4).

[0065] <Evaluation of moisturizing action 1 (humidity retention)>

A use test using the external preparation for skin of Example 3 was carried out, and the moisturizing property of the spring plant extract was evaluated. At that time, the genus plant extract formulated in Example 1 was replaced with a 50% mass ethanol aqueous solution, and the genus plant extract formulated in Comparative Example 1 and Example 3 was replaced with a 50% mass ethanol aqueous solution. A test was conducted in the same manner as Comparative Example 2 using the sample.

[0066] A group of 15 male and female panelists, and each sample was blinded to 4 groups of panelists. It was used for a month, and was evaluated in three stages: “feel”, “can't say”, or “do not feel”. Table 9 shows the number of panelists that obtained each evaluation.

[Table 9]

[0068] From Table 9, it was found that 80% or more of panelists could feel clear moisturizing property in the use group of the examples in which the extract of the spring family plant was blended. From this, it has been clarified that the spring-like plant extract has an excellent moisturizing action.

[0069] <Evaluation of moisturizing effect 2 (rough skin, skin texture, skin transparency)>

A use test using the external preparation for skin of Example 3 and Comparative Examples 1 and 2 was performed, and the improvement effect of these symptoms was evaluated with respect to rough skin, skin texture and skin transparency.

[0070] Each sample has rough skin, smooth skin, and clear skin! / A group of 20 to 50-year-old male and female panelists who have troubles. Each was used for 1 month with blinds, and changes in skin condition before and after use were observed and evaluated. As indicators of skin condition, rough skin, skin texture and skin transparency were evaluated in three stages: “Improved”, “Slightly improved” and “No change”. The rough skin and the transparency of the skin were evaluated visually, and the texture of the skin was observed using a microscope. Table 10 shows the number of panelists that obtained each evaluation.

[0071] [Table 10] Item Evaluation Example 1 Example 3 Comparative Example 1 Comparative Example 2

Improvement 16 15 3 2

Rough skin slightly improved 3 4 5 4

No change 1 1 12 14

Improvement 15 15 4 4

Slight texture improvement 2 4 5 4

No change 3 1 1 1 12

Improvement 16 16 5 4

Transparency Slightly improved 3 2 4 5

No change 1 2 1 1 1 1 From Table 10, it was found that more than half of the panelists did not show improvement in rough skin, skin texture, and skin transparency in comparative examples 1 and 2 that did not contain the extract of the springfish plant. In the example use group containing the extract, a clear improvement was observed in more than 70% of the panelists. From this, it has been found that the spring-like plant extract has an excellent effect on improving skin symptoms in terms of rough skin, skin texture, and skin transparency.

From the above, it has been clarified that the spring plant extract has an excellent moisturizing action.

Claims

The scope of the claims

[1] A moisturizer comprising as an active ingredient one or more plants selected from Osmundaceae plants or extracts thereof.

[2] A cell activator comprising, as an active ingredient, one or more plants selected from Osmundaceae plants or extracts thereof.

[3] A whitening agent containing as an active ingredient one or two or more extracts from Osmundaceae.

[4] Neutral fat accumulation inhibitor containing one or more plants selected from the spring power family (Q ^ mundae ^) or an extract thereof as an active ingredient.

[5] Antioxidants containing as an active ingredient an extract of one or two or more kinds of pork from Osmundaceae.

[6] A topical skin preparation characterized by containing one or more plants selected from the family Qmimdas ^ or an extract thereof.