+

WO2006110309A3 - Transgenic mice expressing a unique b cell population and methods of use - Google Patents

Transgenic mice expressing a unique b cell population and methods of use Download PDF

Info

Publication number
WO2006110309A3
WO2006110309A3 PCT/US2006/011105 US2006011105W WO2006110309A3 WO 2006110309 A3 WO2006110309 A3 WO 2006110309A3 US 2006011105 W US2006011105 W US 2006011105W WO 2006110309 A3 WO2006110309 A3 WO 2006110309A3
Authority
WO
WIPO (PCT)
Prior art keywords
transgenic mice
unique
methods
transgenic
cell population
Prior art date
Application number
PCT/US2006/011105
Other languages
French (fr)
Other versions
WO2006110309A2 (en
WO2006110309A9 (en
Inventor
Patrick Swanson
Original Assignee
Univ Creighton
Patrick Swanson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Creighton, Patrick Swanson filed Critical Univ Creighton
Publication of WO2006110309A2 publication Critical patent/WO2006110309A2/en
Publication of WO2006110309A3 publication Critical patent/WO2006110309A3/en
Publication of WO2006110309A9 publication Critical patent/WO2006110309A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0381—Animal model for diseases of the hematopoietic system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention includes transgenic animals, such as transgenic mice, that express a dominant-negative form of RAG-I during the transitional stage of B lymphocyte development, permitting initial antigen receptor gene rearrangement but blocking receptor editing and/or receptor revision that occurs during later periods in development. In one aspect the transgenic animal includes in its genome an exogenous polynucleotide includes a coding sequence encoding a catalytically defective RAG-I polypeptide, or an analog thereof. The present invention also provides methods of making and using such transgenic animals.
PCT/US2006/011105 2005-03-26 2006-03-27 Transgenic mice expressing a unique b cell population and methods of use WO2006110309A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66540605P 2005-03-26 2005-03-26
US60/665,406 2005-03-26

Publications (3)

Publication Number Publication Date
WO2006110309A2 WO2006110309A2 (en) 2006-10-19
WO2006110309A3 true WO2006110309A3 (en) 2007-01-18
WO2006110309A9 WO2006110309A9 (en) 2007-04-19

Family

ID=37087489

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/011105 WO2006110309A2 (en) 2005-03-26 2006-03-27 Transgenic mice expressing a unique b cell population and methods of use

Country Status (1)

Country Link
WO (1) WO2006110309A2 (en)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FURUSAWA T ET AL: "Catalytic RAG1 mutants obstruct V(D)J recombination in vitro and in vivo.", MOLECULAR IMMUNOLOGY, vol. 39, no. 14, May 2003 (2003-05-01), pages 871 - 878, XP002407203, ISSN: 0161-5890 *
KIM D R ET AL: "Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase", GENES AND DEVELOPMENT, vol. 13, no. 23, December 1999 (1999-12-01), pages 3070 - 3080, XP002407205, ISSN: 0890-9369 *
LANDREE M A ET AL: "Mutational analysis of RAG1 and RAG2 identifies three catalytic amino acids in RAG1 critical for both cleavage steps of V(D)J recombination", GENES AND DEVELOPMENT, vol. 13, no. 23, December 1999 (1999-12-01), pages 3059 - 3069, XP002407204, ISSN: 0890-9369 *
PARKS K W ET AL: "Transgenic mice expressing catalytically inactive RAG-1 exhibit hypogammaglobulinemia and altered B cell development, but not autoreactivity", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 117, no. 2, Suppl. S, February 2006 (2006-02-01), & 62ND ANNUAL MEETING OF THE AMERICAN-ACADEMY-OF-ALLERGY-ASTHMA-AND-IMM UNOLOGY; MIAMI BEACH, FL, USA; MARCH 03 -07, 2006, pages S100, XP002407202, ISSN: 0091-6749 *

Also Published As

Publication number Publication date
WO2006110309A2 (en) 2006-10-19
WO2006110309A9 (en) 2007-04-19

Similar Documents

Publication Publication Date Title
HK1103746A1 (en) Antibodies to erythropoietin receptor and uses thereof
MX2007007602A (en) Glp-1 agonists, compositions, methods and uses.
WO2005108615A3 (en) Nucleic-acid programmable protein arrays
SG151315A1 (en) Human glp-1 mimetibodies, compositions, methods and uses
WO2006085961A3 (en) Anti-mcp-1 antibodies, compositions, methods and uses
WO2005113585A3 (en) Modified tgf-beta superfamily polypeptides
NO20025402D0 (en) Modified ES cells and ES-specific gene
HK1118458A1 (en) Anti-il-23 antibodies, compositions, methods and uses
EP1352961A4 (en) Synovial cell protein
WO2008005290A3 (en) Methods for testing anti-thrombotic agents
JO3058B1 (en) Anti-IL-6 Antibodies,Compositions,Methods and uses
WO2005028511A3 (en) Anti-amyloid antibodies, compositions, methods and uses
TW200716174A (en) Anti-MCP-1 antibodies, compositions, methods and uses
WO2005102034A8 (en) Regulatory sequences for expressing gene products in plant reproductive tissue
WO2009034158A3 (en) Transgenic mice
NO20072499L (en) Melanocortin receptor binding mimetic substances, compositions, methods and applications
IN2009CN03372A (en)
WO2006094124A3 (en) Compositions and methods for treatment of autoimmune and related diseases
EP2084534A4 (en) METHODS OF IDENTIFYING, ISOLATING AND USING ENDOCRINE PROGENITOR CELLS FROM ADULT HUMAN PANCREAS
WO2007052096A3 (en) Production of bone morphogenic proteins (bmps) in transgenic mammals
WO2006110309A3 (en) Transgenic mice expressing a unique b cell population and methods of use
DE60040413D1 (en) CELLS WITH DEFICIENT MELANOCORTIN-3 RECEPTOR, TRANSGENIC MICE, AND METHOD FOR SELECTION OF SUBSTANCES WHICH CAN REGULATE THE BODY WEIGHT
WO2003059932A3 (en) Methods of inducing or enhancing connective tissue repair
WO2008057233A3 (en) Models of erythropoiesis
WO2007013897A3 (en) Transgenic mouse expressing a polynucleotide encoding human tissue factor

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: RU

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 06758203

Country of ref document: EP

Kind code of ref document: A2

122 Ep: pct application non-entry in european phase

Ref document number: 06758203

Country of ref document: EP

Kind code of ref document: A2

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载