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WO2006042327A3 - Plant-produced recombinant aprotinin and aprotinin variants - Google Patents

Plant-produced recombinant aprotinin and aprotinin variants Download PDF

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Publication number
WO2006042327A3
WO2006042327A3 PCT/US2005/037097 US2005037097W WO2006042327A3 WO 2006042327 A3 WO2006042327 A3 WO 2006042327A3 US 2005037097 W US2005037097 W US 2005037097W WO 2006042327 A3 WO2006042327 A3 WO 2006042327A3
Authority
WO
WIPO (PCT)
Prior art keywords
aprotinin
variants
plant
produced recombinant
immunological
Prior art date
Application number
PCT/US2005/037097
Other languages
French (fr)
Other versions
WO2006042327A2 (en
Inventor
Fakhrieh S Vojdani
Kenneth E Palmer
Stephen J Garger
Gregory P Pogue
Original Assignee
Large Scale Biology Corp
Fakhrieh S Vojdani
Kenneth E Palmer
Stephen J Garger
Gregory P Pogue
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Large Scale Biology Corp, Fakhrieh S Vojdani, Kenneth E Palmer, Stephen J Garger, Gregory P Pogue filed Critical Large Scale Biology Corp
Publication of WO2006042327A2 publication Critical patent/WO2006042327A2/en
Publication of WO2006042327A3 publication Critical patent/WO2006042327A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to plant produced native aprotinin and aprotinin variants having enzyme-inhibitory, immunological and pharmacokinetic properties and their preparation. In a preferred method a recombinant RNA plant virus is used to express native aprotinin + variants thereof in Nicotiana plants.
PCT/US2005/037097 2004-10-12 2005-10-12 Plant-produced recombinant aprotinin and aprotinin variants WO2006042327A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61848504P 2004-10-12 2004-10-12
US60/618,485 2004-10-12
US63521404P 2004-12-10 2004-12-10
US60/635,214 2004-12-10

Publications (2)

Publication Number Publication Date
WO2006042327A2 WO2006042327A2 (en) 2006-04-20
WO2006042327A3 true WO2006042327A3 (en) 2009-04-16

Family

ID=36149040

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/037097 WO2006042327A2 (en) 2004-10-12 2005-10-12 Plant-produced recombinant aprotinin and aprotinin variants

Country Status (1)

Country Link
WO (1) WO2006042327A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014097733A1 (en) * 2012-12-21 2014-06-26 株式会社ＵｎｉＢｉｏ Method for manufacturing human epidermal cell growth factor
PL434960A1 (en) * 2020-08-13 2022-02-14 Pharmfactor Spółka Z Ograniczoną Odpowiedzialnością Method of obtaining aprotinin and product obtained in this way

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824870A (en) * 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
US6482798B2 (en) * 1996-07-25 2002-11-19 Bayer Aktiengesellschaft Aprotinin variants having improved properties
US20040106198A1 (en) * 2002-07-16 2004-06-03 Large Scale Biology Corporation Inhibition of peptide cleavage in plants

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824870A (en) * 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
US6482798B2 (en) * 1996-07-25 2002-11-19 Bayer Aktiengesellschaft Aprotinin variants having improved properties
US20040106198A1 (en) * 2002-07-16 2004-06-03 Large Scale Biology Corporation Inhibition of peptide cleavage in plants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CONCETTI ET AL.: "Selective Oxidation of Methionine Residues in Kunitz-Type protease inhibitors", BIOL. CHEM. HOPPE-SEYLER., vol. 370, July 1989 (1989-07-01), pages 723 - 728 *

Also Published As

Publication number Publication date
WO2006042327A2 (en) 2006-04-20

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