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WO2004027018A3 - Dosage de cellule kinase a adhesion focale inductible - Google Patents

Dosage de cellule kinase a adhesion focale inductible Download PDF

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Publication number
WO2004027018A3
WO2004027018A3 PCT/IB2003/003968 IB0303968W WO2004027018A3 WO 2004027018 A3 WO2004027018 A3 WO 2004027018A3 IB 0303968 W IB0303968 W IB 0303968W WO 2004027018 A3 WO2004027018 A3 WO 2004027018A3
Authority
WO
WIPO (PCT)
Prior art keywords
fak
cell assay
focal adhesion
adhesion kinase
kinase cell
Prior art date
Application number
PCT/IB2003/003968
Other languages
English (en)
Other versions
WO2004027018A2 (fr
Inventor
Walter Gregory Roberts
Pamela Matthews Whalen
Ethan James Tekly Ung
Original Assignee
Pfizer Prod Inc
Walter Gregory Roberts
Pamela Matthews Whalen
Ethan James Tekly Ung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Prod Inc, Walter Gregory Roberts, Pamela Matthews Whalen, Ethan James Tekly Ung filed Critical Pfizer Prod Inc
Priority to CA002497434A priority Critical patent/CA2497434A1/fr
Priority to EP03797456A priority patent/EP1546372A4/fr
Priority to JP2004537413A priority patent/JP2006500022A/ja
Priority to AU2003259498A priority patent/AU2003259498A1/en
Publication of WO2004027018A2 publication Critical patent/WO2004027018A2/fr
Publication of WO2004027018A3 publication Critical patent/WO2004027018A3/fr
Priority to NO20051869A priority patent/NO20051869L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un dosage cellulaire qui exploite le biologie de FAK conjointement avec un système d'expression génique inductible afin de contrôler depuis l'extérieur l'expression de FAK et la phosphorylation de FAK du résidu de tyrosine au niveau de la position 397 (Y397). Ce dosage de cellule kinase de l'invention est flexible et peut mesurer le phosphorylation de FAK au niveau de Y397, la phosphorylation de FAK totale, identifier les protéines de FAK mutantes et mesurer une combinaison de protéine et de phosphotyrosine.
PCT/IB2003/003968 2002-09-19 2003-09-08 Dosage de cellule kinase a adhesion focale inductible WO2004027018A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002497434A CA2497434A1 (fr) 2002-09-19 2003-09-08 Dosage de cellule kinase a adhesion focale inductible
EP03797456A EP1546372A4 (fr) 2002-09-19 2003-09-08 Dosage de cellule kinase a adhesion focale inductible
JP2004537413A JP2006500022A (ja) 2002-09-19 2003-09-08 誘導可能な局所接着キナーゼ細胞アッセイ
AU2003259498A AU2003259498A1 (en) 2002-09-19 2003-09-08 Inducible focal adhesion kinase cell assay
NO20051869A NO20051869L (no) 2002-09-19 2005-04-18 Celleanalyse av induserbare focal adhesjonskinase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41207802P 2002-09-19 2002-09-19
US60/412,078 2002-09-19

Publications (2)

Publication Number Publication Date
WO2004027018A2 WO2004027018A2 (fr) 2004-04-01
WO2004027018A3 true WO2004027018A3 (fr) 2004-09-16

Family

ID=32030798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2003/003968 WO2004027018A2 (fr) 2002-09-19 2003-09-08 Dosage de cellule kinase a adhesion focale inductible

Country Status (9)

Country Link
EP (1) EP1546372A4 (fr)
JP (1) JP2006500022A (fr)
KR (1) KR20050052663A (fr)
CN (1) CN1681939A (fr)
AU (1) AU2003259498A1 (fr)
CA (1) CA2497434A1 (fr)
NO (1) NO20051869L (fr)
RU (1) RU2005107701A (fr)
WO (1) WO2004027018A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100934706B1 (ko) * 2006-12-07 2009-12-31 재단법인서울대학교산학협력재단 Ｔｍ４ｓｆ５의 기능을 저해하는 항암물질의 스크리닝 방법및 칼콘계 화합물을 함유하는 항암조성물
WO2009046129A2 (fr) * 2007-10-01 2009-04-09 Columbia University Procédés de traitement du syndrome de détresse respiratoire de l'adulte
CN109112213B (zh) * 2017-06-22 2021-01-12 中国科学院动物研究所 检测黏着斑激酶结构变异体的pcr引物及其检测方法与应用
KR102007450B1 (ko) 2018-05-31 2019-08-05 한국과학기술연구원 대장암 치료제 발굴을 위한 신규 표적 치료 작용점의 스크리닝 방법 및 이를 이용하여 선별한 대장암 치료 예후 바이오마커
KR102408538B1 (ko) * 2020-10-29 2022-06-15 한국과학기술연구원 Fak 활성 억제제를 스크리닝하기 위한 조성물, 키트 및 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133031A (en) * 1999-08-19 2000-10-17 Isis Pharmaceuticals Inc. Antisense inhibition of focal adhesion kinase expression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6395506B1 (en) * 1991-04-18 2002-05-28 Becton, Dickinson And Company Device for monitoring cells
AU5596998A (en) * 1996-12-11 1998-07-03 New York University Medical Center Pyk2 related products and methods
US20010055585A1 (en) * 1997-12-03 2001-12-27 William G. Cance Frnk proteins in the treatment of tumor cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133031A (en) * 1999-08-19 2000-10-17 Isis Pharmaceuticals Inc. Antisense inhibition of focal adhesion kinase expression

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HEIDKAMP M.C.: "Activation of focal adhesion kinase by protein kinase C.epsilon. in neonatal rat ventricular myocytes", AM. J. PHYSIOL. HEART CIRC. PHYSIOL., vol. 285, 26 June 2003 (2003-06-26), pages H1684 - H1696, XP002979010 *
RODRIGUEZ-FERNANDEZ J.L.: "Bombesin, Vasopressin, Lysophosphatidic Acid, and Sphingosylphosphorylcholine Induce Focal Adhesion Kinase Activation in Intact Swiss 3T3 Cells", J. BIOL. CHEM., vol. 273, no. 30, 24 July 1998 (1998-07-24), pages 19321 - 19328, XP002978736 *
See also references of EP1546372A4 *

Also Published As

Publication number Publication date
EP1546372A2 (fr) 2005-06-29
JP2006500022A (ja) 2006-01-05
CN1681939A (zh) 2005-10-12
EP1546372A4 (fr) 2006-08-23
AU2003259498A1 (en) 2004-04-08
NO20051869D0 (no) 2005-04-18
CA2497434A1 (fr) 2004-04-01
KR20050052663A (ko) 2005-06-03
NO20051869L (no) 2005-06-16
WO2004027018A2 (fr) 2004-04-01
RU2005107701A (ru) 2005-08-27

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