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WO2004027018A3 - Inducible focal adhesion kinase cell assay - Google Patents

Inducible focal adhesion kinase cell assay Download PDF

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Publication number
WO2004027018A3
WO2004027018A3 PCT/IB2003/003968 IB0303968W WO2004027018A3 WO 2004027018 A3 WO2004027018 A3 WO 2004027018A3 IB 0303968 W IB0303968 W IB 0303968W WO 2004027018 A3 WO2004027018 A3 WO 2004027018A3
Authority
WO
WIPO (PCT)
Prior art keywords
fak
cell assay
focal adhesion
adhesion kinase
kinase cell
Prior art date
Application number
PCT/IB2003/003968
Other languages
French (fr)
Other versions
WO2004027018A2 (en
Inventor
Walter Gregory Roberts
Pamela Matthews Whalen
Ethan James Tekly Ung
Original Assignee
Pfizer Prod Inc
Walter Gregory Roberts
Pamela Matthews Whalen
Ethan James Tekly Ung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Prod Inc, Walter Gregory Roberts, Pamela Matthews Whalen, Ethan James Tekly Ung filed Critical Pfizer Prod Inc
Priority to CA002497434A priority Critical patent/CA2497434A1/en
Priority to EP03797456A priority patent/EP1546372A4/en
Priority to JP2004537413A priority patent/JP2006500022A/en
Priority to AU2003259498A priority patent/AU2003259498A1/en
Publication of WO2004027018A2 publication Critical patent/WO2004027018A2/en
Publication of WO2004027018A3 publication Critical patent/WO2004027018A3/en
Priority to NO20051869A priority patent/NO20051869L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The cell-based assay of the present invention exploits the biology of FAK in conjunction an inducible gene expression system to exogenously control FAK expression and FAK phosphorylation at the tyrosine residue at position 397 (Y397). The cell-based assay of the present invention is flexible and can measure FAK phosphorylation at Y397, total FAK phosphorylation, identify mutant FAK proteins and measure a combination of protein and phosphotyrosine.
PCT/IB2003/003968 2002-09-19 2003-09-08 Inducible focal adhesion kinase cell assay WO2004027018A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002497434A CA2497434A1 (en) 2002-09-19 2003-09-08 Inducible focal adhesion kinase cell assay
EP03797456A EP1546372A4 (en) 2002-09-19 2003-09-08 Inducible focal adhesion kinase cell assay
JP2004537413A JP2006500022A (en) 2002-09-19 2003-09-08 Inducible local adhesion kinase cell assay
AU2003259498A AU2003259498A1 (en) 2002-09-19 2003-09-08 Inducible focal adhesion kinase cell assay
NO20051869A NO20051869L (en) 2002-09-19 2005-04-18 Cell analysis of inducible focal adhesion kinase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41207802P 2002-09-19 2002-09-19
US60/412,078 2002-09-19

Publications (2)

Publication Number Publication Date
WO2004027018A2 WO2004027018A2 (en) 2004-04-01
WO2004027018A3 true WO2004027018A3 (en) 2004-09-16

Family

ID=32030798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2003/003968 WO2004027018A2 (en) 2002-09-19 2003-09-08 Inducible focal adhesion kinase cell assay

Country Status (9)

Country Link
EP (1) EP1546372A4 (en)
JP (1) JP2006500022A (en)
KR (1) KR20050052663A (en)
CN (1) CN1681939A (en)
AU (1) AU2003259498A1 (en)
CA (1) CA2497434A1 (en)
NO (1) NO20051869L (en)
RU (1) RU2005107701A (en)
WO (1) WO2004027018A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100934706B1 (en) * 2006-12-07 2009-12-31 재단법인서울대학교산학협력재단 Method for Screening Anti-cancer Compounds Inhibiting Functions of TM4SF5 and Anti-cancer Composition Containing Chalcone Compounds
WO2009046129A2 (en) * 2007-10-01 2009-04-09 Columbia University Methods for treating adult respiratory distress syndrome
CN109112213B (en) * 2017-06-22 2021-01-12 中国科学院动物研究所 PCR primer for detecting focal adhesion kinase structural variant and detection method and application thereof
KR102007450B1 (en) 2018-05-31 2019-08-05 한국과학기술연구원 Screening method for new therapeuric targets of drug discovery for colon cancer and prognostic biomarkers for colon cancer screened by using the same
KR102408538B1 (en) * 2020-10-29 2022-06-15 한국과학기술연구원 Composition, kit and method for screening FAK inhibitor

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133031A (en) * 1999-08-19 2000-10-17 Isis Pharmaceuticals Inc. Antisense inhibition of focal adhesion kinase expression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6395506B1 (en) * 1991-04-18 2002-05-28 Becton, Dickinson And Company Device for monitoring cells
AU5596998A (en) * 1996-12-11 1998-07-03 New York University Medical Center Pyk2 related products and methods
US20010055585A1 (en) * 1997-12-03 2001-12-27 William G. Cance Frnk proteins in the treatment of tumor cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133031A (en) * 1999-08-19 2000-10-17 Isis Pharmaceuticals Inc. Antisense inhibition of focal adhesion kinase expression

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HEIDKAMP M.C.: "Activation of focal adhesion kinase by protein kinase C.epsilon. in neonatal rat ventricular myocytes", AM. J. PHYSIOL. HEART CIRC. PHYSIOL., vol. 285, 26 June 2003 (2003-06-26), pages H1684 - H1696, XP002979010 *
RODRIGUEZ-FERNANDEZ J.L.: "Bombesin, Vasopressin, Lysophosphatidic Acid, and Sphingosylphosphorylcholine Induce Focal Adhesion Kinase Activation in Intact Swiss 3T3 Cells", J. BIOL. CHEM., vol. 273, no. 30, 24 July 1998 (1998-07-24), pages 19321 - 19328, XP002978736 *
See also references of EP1546372A4 *

Also Published As

Publication number Publication date
EP1546372A2 (en) 2005-06-29
JP2006500022A (en) 2006-01-05
CN1681939A (en) 2005-10-12
EP1546372A4 (en) 2006-08-23
AU2003259498A1 (en) 2004-04-08
NO20051869D0 (en) 2005-04-18
CA2497434A1 (en) 2004-04-01
KR20050052663A (en) 2005-06-03
NO20051869L (en) 2005-06-16
WO2004027018A2 (en) 2004-04-01
RU2005107701A (en) 2005-08-27

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