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WO2004026036A1 - Produit destine a la desinfection et a la conservation de poissons, de produits a base de poissons, de denrees et d'aliments - Google Patents

Produit destine a la desinfection et a la conservation de poissons, de produits a base de poissons, de denrees et d'aliments Download PDF

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Publication number
WO2004026036A1
WO2004026036A1 PCT/NO2003/000302 NO0300302W WO2004026036A1 WO 2004026036 A1 WO2004026036 A1 WO 2004026036A1 NO 0300302 W NO0300302 W NO 0300302W WO 2004026036 A1 WO2004026036 A1 WO 2004026036A1
Authority
WO
WIPO (PCT)
Prior art keywords
weight
ethanol
fish
kdf
bacteria
Prior art date
Application number
PCT/NO2003/000302
Other languages
English (en)
Inventor
Tom Granli
Henrik HØYVIK
Rune Christiansen
Jostein Braaten
Original Assignee
Norsk Hydro Asa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Norsk Hydro Asa filed Critical Norsk Hydro Asa
Priority to AU2003263688A priority Critical patent/AU2003263688A1/en
Publication of WO2004026036A1 publication Critical patent/WO2004026036A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B4/00—Preservation of meat, sausages, fish or fish products
    • A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
    • A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
    • A23B4/20—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729—Organic compounds; Microorganisms; Enzymes
    • A23B2/742—Organic compounds containing oxygen
    • A23B2/746—Organic compounds containing oxygen with singly-bound oxygen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729—Organic compounds; Microorganisms; Enzymes
    • A23B2/742—Organic compounds containing oxygen
    • A23B2/75—Organic compounds containing oxygen with doubly-bound oxygen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729—Organic compounds; Microorganisms; Enzymes
    • A23B2/742—Organic compounds containing oxygen
    • A23B2/754—Organic compounds containing oxygen containing carboxyl groups
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B4/00—Preservation of meat, sausages, fish or fish products
    • A23B4/12—Preserving with acids; Acid fermentation
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof

Definitions

  • the present invention relates to a product for the disinfection and preservation of fish, fish products, foodstuffs and feed.
  • the pH of the solutions is preferably around 3, and to obtain said pH, HCl is added to acidify the solutions.
  • the addition of HCl is a clear drawback in food and feed applications.
  • the addition of HCl might also lead to corrosion on equipment.
  • the amounts of organic acids are low in relation to the amount of ethanol.
  • the main object of the invention was to provide a product, which would effectively kill and inhibit the growth of Listeria monocytogenes, Salmonella enteritidis, Bacillus cereus and Staphylococcus aureus subsp. aureus in fish, fish products, foodstuffs and feed.
  • Another object was that the product should be acceptable in fish products.
  • KDF Potassium diformate
  • the effective amount of potassium diformate was found to be in the range 5 - 15 weight %, and the amount of ethanol should be in the range 1 - 5 weight %, the balance being water. Contrary to that taught by Barker et al, it was found that the product would be effective even at pH 4.5, and that the pH should preferably be in the range 4.0 - 4.5.
  • the present invention will in its widest scope comprise a product for the disinfection and preservation of fish, fish products, foodstuffs and feed comprising formates and ethanol, where said formate is potassium diformate and the amount of potassium diformate is 1 - 25 weight %, and the amount of ethanol is 0.5 - 10 weight %, the balance being water.
  • the amount of potassium diformate is preferably 5 - 15 weight %, and the amount of ethanol is preferably 1 - 5 weight %.
  • the pH of the solution is 3.5 - 4.5, preferably 4.0 - 4.5.
  • Table 1 shows CCUG number, gram staining and growth conditions for the bacteria that were used in Examples 1 - 4.
  • the inoculum were made from bacteria grown on agar plates, which were diluted by physiological salt water and inoculated into a test solution of KDF/ethanol to ensure a bacterial concentration of 10 8 - 10 9 cfu/ml. Samples of 100 ⁇ l of the inoculated test solution were taken after 1, 3, 5, 10 and 30 minutes and diluted 1:100 with physiological salt water. The samples were diluted immediately after the sampling to prevent further influence of KDF on the microorganisms. Killing in the different solutions was determined by placing a 10 fold dilution series of the test solutions on plates.
  • the detection limit in the tests was 1000 cfu/ml test solution. A reduction in the number of bacteria of 5 log units could be observed.
  • the bacteria were added to solutions of different potassium diformate (KDF) concentrations, with and without ethanol. Samples were taken at different points of time and spread on agar to quantify the microorganisms. For the different KDF solutions the killing of the microorganisms was investigated as a function of time.
  • KDF potassium diformate
  • Table 2 shows time in minutes before complete killing effect of potassium diformate (KDF) and ethanol on Bacillus cereus was achieved.
  • the KDF/ethanol solutions had good killing effect on Bacillus cereus.
  • the solution with 5 weight % KDF / 1 weight % ethanol showed full killing effect after 5 minutes.
  • For 5 weight % KDF / 3 weight % ethanol and 5 weight % KDF / 5 weight % ethanol no bacteria were observed after 3 minutes.
  • For solutions with 10 weight % KDF bacteria were killed after 3 - 5 minutes, and with 10 weight % KDF / 1 weight % ethanol, bacteria were killed after 3 - 10 minutes.
  • solutions with 10 weight % KDF / 3 weight % ethanol and solutions with 15 weight % KDF, with and without ethanol full killing effect was observed after 1 minute.
  • Table 3 shows time in minutes before complete killing effect of potassium diformate (KDF) and ethanol on Salmonella enteritidis was achieved.
  • the KDF/ethanol solutions had good killing effect on Salmonella enteritidis. After ten minutes, 5 weight % KDF with 1 weight % ethanol had full killing effect on Salmonella, 5 weight % KDF / 3 weight % ethanol and 5 weight % KDF / 5 weight % ethanol killed the bacteria after three minutes. For samples treated with 10 weight % and 15 weight % KDF with and without ethanol, there was full killing effect after one minute.
  • Table 4 shows time in minutes before complete killing effect of potassium diformate (KDF) and ethanol on Staphylococcus was achieved.
  • KDF potassium diformate
  • Table 6 shows the different solutions of KDF and ethanol that were tested.
  • Tables 7, 8 and 9 show number of Listeria bacteria (cfu/g) after immersing salmon gills, abdomen and slime in different solutions (A, B, C, D, E) of KDF / ethanol, with and without washing.
  • the control was fish infected with Listeria, but not treated with KDF.
  • Table 10 shows the number of Listeria bacteria (cfu/g) after immersing salmon for 10 min in the solutions A and E, with and without washing. Fish infected with Listeria, but not treated with KDF was used as control. The tests were taken after the fish had been stored for 12 days at 2 °C.
  • the test showed that the solutions had good effect against Listeria when infected fish was stored over a prolonged time (12 days).
  • the solutions of KDF and ethanol efficiently inhibited the growth of Listeria and could thus be well suited as a preservative agent for fish, fish products, feedstuffs and feed to be stored.
  • the product according to the invention has an anti microbial effect and with the results from the tests in Examples 1 - 3 it is highly probable that it will also be applicable and effective on products infected with Bacillus, Salmonella and Staphylococcus.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Nutrition Science (AREA)
  • Animal Husbandry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

L'invention concerne un produit aqueux destiné à la désinfection et à la conservation de poissons, de produits à base de poissons, de denrées et d'aliments et comprenant des formiates et de l'éthanol, ledit formiate étant du diformiate de potassium et la quantité de celui-ci étant comprise entre 1 et 25 % en poids, de préférence entre 5 et 15 % en poids, et la quantité d'éthanol étant comprise entre 0,5 et 10 % en poids, de préférence entre 1 et 5 % en poids. Le pH de la solution est compris entre 3,5 et 4,5, de préférence entre 4,0 et 4,5.
PCT/NO2003/000302 2002-09-20 2003-08-29 Produit destine a la desinfection et a la conservation de poissons, de produits a base de poissons, de denrees et d'aliments WO2004026036A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003263688A AU2003263688A1 (en) 2002-09-20 2003-08-29 Product for the disinfection and preservation of fish, fish products, foodstuffs and feed

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NO20024501A NO317082B1 (no) 2002-09-20 2002-09-20 Produkt for desinfisering og konservering av fisk, fiskeprodukter, matvarer og fôr
NO20024501 2002-09-20

Publications (1)

Publication Number Publication Date
WO2004026036A1 true WO2004026036A1 (fr) 2004-04-01

Family

ID=19914017

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NO2003/000302 WO2004026036A1 (fr) 2002-09-20 2003-08-29 Produit destine a la desinfection et a la conservation de poissons, de produits a base de poissons, de denrees et d'aliments

Country Status (4)

Country Link
AU (1) AU2003263688A1 (fr)
NO (1) NO317082B1 (fr)
PE (1) PE20040403A1 (fr)
WO (1) WO2004026036A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2087724A (en) * 1980-09-26 1982-06-03 Ueno Seiyaku Oyo Kenkyujo Kk Bactericide or foods and food processing machines or utensils
WO1993016603A1 (fr) * 1992-02-27 1993-09-02 Kemira Oy Agent de conservation pour fourrages verts
WO2001019207A1 (fr) * 1999-09-15 2001-03-22 Norsk Hydro Asa Conservateur/acidifiant aqueux

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO300192B1 (no) * 1995-08-07 1997-04-28 Sildolje & Sildemelind Forsk Fremgangsmate for kjoling og konservering av fisk samt produkt fra fisk behandlet ifolge fremgangsmaten

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2087724A (en) * 1980-09-26 1982-06-03 Ueno Seiyaku Oyo Kenkyujo Kk Bactericide or foods and food processing machines or utensils
WO1993016603A1 (fr) * 1992-02-27 1993-09-02 Kemira Oy Agent de conservation pour fourrages verts
WO2001019207A1 (fr) * 1999-09-15 2001-03-22 Norsk Hydro Asa Conservateur/acidifiant aqueux

Also Published As

Publication number Publication date
AU2003263688A1 (en) 2004-04-08
NO20024501D0 (no) 2002-09-20
PE20040403A1 (es) 2004-06-25
NO317082B1 (no) 2004-08-02

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