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WO2004018998A3 - Procede permettant d'utiliser l'amplification en chaine par polymerase en temps reel - Google Patents

Procede permettant d'utiliser l'amplification en chaine par polymerase en temps reel Download PDF

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Publication number
WO2004018998A3
WO2004018998A3 PCT/US2003/026177 US0326177W WO2004018998A3 WO 2004018998 A3 WO2004018998 A3 WO 2004018998A3 US 0326177 W US0326177 W US 0326177W WO 2004018998 A3 WO2004018998 A3 WO 2004018998A3
Authority
WO
WIPO (PCT)
Prior art keywords
time pcr
real
qpcr
present
quantitative
Prior art date
Application number
PCT/US2003/026177
Other languages
English (en)
Other versions
WO2004018998A2 (fr
Inventor
Daniel C Edelman
Original Assignee
Univ Maryland
Daniel C Edelman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland, Daniel C Edelman filed Critical Univ Maryland
Priority to AU2003272230A priority Critical patent/AU2003272230A1/en
Publication of WO2004018998A2 publication Critical patent/WO2004018998A2/fr
Publication of WO2004018998A3 publication Critical patent/WO2004018998A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé permettant de réaliser une analyse quantitative ou qualitative d'un échantillon brut biologique ou prélevé dans l'environnement, au moyen d'une amplification en chaîne par polymérase quantitative en temps réel (QPCR).
PCT/US2003/026177 2002-08-20 2003-08-20 Procede permettant d'utiliser l'amplification en chaine par polymerase en temps reel WO2004018998A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003272230A AU2003272230A1 (en) 2002-08-20 2003-08-20 Method using real-time pcr

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40462202P 2002-08-20 2002-08-20
US60/404,622 2002-08-20
US42765002P 2002-11-19 2002-11-19
US60/427,650 2002-11-19

Publications (2)

Publication Number Publication Date
WO2004018998A2 WO2004018998A2 (fr) 2004-03-04
WO2004018998A3 true WO2004018998A3 (fr) 2004-06-24

Family

ID=31949865

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/026177 WO2004018998A2 (fr) 2002-08-20 2003-08-20 Procede permettant d'utiliser l'amplification en chaine par polymerase en temps reel

Country Status (2)

Country Link
AU (1) AU2003272230A1 (fr)
WO (1) WO2004018998A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6335119B2 (ja) 2011-07-06 2018-05-30 クエスト ダイアグノスティクス インベストメンツ インコーポレイテッド ウイルスおよび細菌病原体の直接増幅および検出
CN114369683B (zh) * 2021-09-16 2023-07-25 中山大学 马来西亚大虾病毒的检测试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033854A (en) * 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US6447994B1 (en) * 2000-06-20 2002-09-10 The General Hospital Corporation Production of replicative hepatitis C virus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033854A (en) * 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US6447994B1 (en) * 2000-06-20 2002-09-10 The General Hospital Corporation Production of replicative hepatitis C virus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RAVAGGI ET AL.: "Direct PCR amplification of HCV RNA from human serum", PCR METHODS AND APPLICATIONS, vol. 1, 1992, pages 291 - 292, XP008029323 *

Also Published As

Publication number Publication date
WO2004018998A2 (fr) 2004-03-04
AU2003272230A8 (en) 2004-03-11
AU2003272230A1 (en) 2004-03-11

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