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WO2004018998A3 - Method using real-time pcr - Google Patents

Method using real-time pcr Download PDF

Info

Publication number
WO2004018998A3
WO2004018998A3 PCT/US2003/026177 US0326177W WO2004018998A3 WO 2004018998 A3 WO2004018998 A3 WO 2004018998A3 US 0326177 W US0326177 W US 0326177W WO 2004018998 A3 WO2004018998 A3 WO 2004018998A3
Authority
WO
WIPO (PCT)
Prior art keywords
time pcr
real
qpcr
present
quantitative
Prior art date
Application number
PCT/US2003/026177
Other languages
French (fr)
Other versions
WO2004018998A2 (en
Inventor
Daniel C Edelman
Original Assignee
Univ Maryland
Daniel C Edelman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland, Daniel C Edelman filed Critical Univ Maryland
Priority to AU2003272230A priority Critical patent/AU2003272230A1/en
Publication of WO2004018998A2 publication Critical patent/WO2004018998A2/en
Publication of WO2004018998A3 publication Critical patent/WO2004018998A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention demonstrates a method for performing quantitative or qualitative analysis on a crude biological or environmental sample utilizing quantitative real-time PCR (QPCR).
PCT/US2003/026177 2002-08-20 2003-08-20 Method using real-time pcr WO2004018998A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003272230A AU2003272230A1 (en) 2002-08-20 2003-08-20 Method using real-time pcr

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40462202P 2002-08-20 2002-08-20
US60/404,622 2002-08-20
US42765002P 2002-11-19 2002-11-19
US60/427,650 2002-11-19

Publications (2)

Publication Number Publication Date
WO2004018998A2 WO2004018998A2 (en) 2004-03-04
WO2004018998A3 true WO2004018998A3 (en) 2004-06-24

Family

ID=31949865

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/026177 WO2004018998A2 (en) 2002-08-20 2003-08-20 Method using real-time pcr

Country Status (2)

Country Link
AU (1) AU2003272230A1 (en)
WO (1) WO2004018998A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6335119B2 (en) 2011-07-06 2018-05-30 クエスト ダイアグノスティクス インベストメンツ インコーポレイテッド Direct amplification and detection of viral and bacterial pathogens
CN114369683B (en) * 2021-09-16 2023-07-25 中山大学 Detection kit for prawn virus in Malaysia

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033854A (en) * 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US6447994B1 (en) * 2000-06-20 2002-09-10 The General Hospital Corporation Production of replicative hepatitis C virus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6033854A (en) * 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US6447994B1 (en) * 2000-06-20 2002-09-10 The General Hospital Corporation Production of replicative hepatitis C virus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RAVAGGI ET AL.: "Direct PCR amplification of HCV RNA from human serum", PCR METHODS AND APPLICATIONS, vol. 1, 1992, pages 291 - 292, XP008029323 *

Also Published As

Publication number Publication date
WO2004018998A2 (en) 2004-03-04
AU2003272230A8 (en) 2004-03-11
AU2003272230A1 (en) 2004-03-11

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