WO2004076685A3 - Fungal biosensor and assay - Google Patents
Fungal biosensor and assay Download PDFInfo
- Publication number
- WO2004076685A3 WO2004076685A3 PCT/GB2004/000839 GB2004000839W WO2004076685A3 WO 2004076685 A3 WO2004076685 A3 WO 2004076685A3 GB 2004000839 W GB2004000839 W GB 2004000839W WO 2004076685 A3 WO2004076685 A3 WO 2004076685A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- biosensor
- luminescent
- fungal
- assay
- fungi
- Prior art date
Links
- 238000003556 assay Methods 0.000 title abstract 2
- 230000002538 fungal effect Effects 0.000 title abstract 2
- 241000233866 Fungi Species 0.000 abstract 3
- 108010047357 Luminescent Proteins Proteins 0.000 abstract 2
- 102000006830 Luminescent Proteins Human genes 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 241000228257 Aspergillus sp. Species 0.000 abstract 1
- 241000963438 Gaussia <copepod> Species 0.000 abstract 1
- 108060001084 Luciferase Proteins 0.000 abstract 1
- 239000005089 Luciferase Substances 0.000 abstract 1
- 241000088436 Neurospora sp. Species 0.000 abstract 1
- 102000007982 Phosphoproteins Human genes 0.000 abstract 1
- 108010089430 Phosphoproteins Proteins 0.000 abstract 1
- INULNSAIIZKOQE-YOSAUDMPSA-N [(3r,4ar,10ar)-6-methoxy-1-methyl-3,4,4a,5,10,10a-hexahydro-2h-benzo[g]quinolin-3-yl]-[4-(4-nitrophenyl)piperazin-1-yl]methanone Chemical compound O=C([C@@H]1C[C@H]2[C@H](N(C1)C)CC=1C=CC=C(C=1C2)OC)N(CC1)CCN1C1=CC=C([N+]([O-])=O)C=C1 INULNSAIIZKOQE-YOSAUDMPSA-N 0.000 abstract 1
- 239000003623 enhancer Substances 0.000 abstract 1
- 108091006047 fluorescent proteins Proteins 0.000 abstract 1
- 102000034287 fluorescent proteins Human genes 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 238000004020 luminiscence type Methods 0.000 abstract 1
- 108010089433 obelin Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A fungal biosensor comprising luminescent or fluorescent filamentous fungi is provided. The luminescence/fluorescence of the fungi varies depending upon whether the predetermined test substance is present or absent. Typically the gene encoding the luminescent or fluorescent protein is genetically engineered to be under the control of an inducible promoter or enhancer sensitive to the test substance. Suitable fungi include Neurospora Sp., and Aspergillus Sp. Exemplary luminescent proteins include the Gaussia luciferase and the phosphoprotein obelin. An assay using the biosensor is also described.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0304625A GB0304625D0 (en) | 2003-02-28 | 2003-02-28 | Filamentous Fungi |
| GB0304625.7 | 2003-02-28 | ||
| GB0330087.8 | 2003-12-27 | ||
| GB0330087A GB0330087D0 (en) | 2003-12-27 | 2003-12-27 | Biosensor 2 |
| GB0400183.0 | 2004-01-07 | ||
| GB0400183A GB0400183D0 (en) | 2004-01-07 | 2004-01-07 | Biosensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2004076685A2 WO2004076685A2 (en) | 2004-09-10 |
| WO2004076685A3 true WO2004076685A3 (en) | 2005-01-20 |
Family
ID=32931046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/GB2004/000839 WO2004076685A2 (en) | 2003-02-28 | 2004-03-01 | Fungal biosensor and assay |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2004076685A2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2674894C2 (en) * | 2017-01-30 | 2018-12-13 | Общество с ограниченной ответственностью "ПЛАНТА" | New luciferases and methods of their use |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998021347A1 (en) * | 1996-11-15 | 1998-05-22 | E.I. Du Pont De Nemours And Company | A small volume, highly sensitive method for detecting environmental insults |
| WO1999049019A2 (en) * | 1998-03-27 | 1999-09-30 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
| US6017722A (en) * | 1991-04-04 | 2000-01-25 | Board Of Regents, The University Of Texas System | Luminous bacteria and methods for the isolation, identification and quantitation of toxicants |
| WO2000014267A1 (en) * | 1998-09-09 | 2000-03-16 | Aberdeen University | Biosensor |
| WO2000037665A1 (en) * | 1998-12-22 | 2000-06-29 | The University Of Georgia Research Foundation | Method and system for the continuous synthesis of natural products using immobilized fungal spores |
-
2004
- 2004-03-01 WO PCT/GB2004/000839 patent/WO2004076685A2/en active Application Filing
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6017722A (en) * | 1991-04-04 | 2000-01-25 | Board Of Regents, The University Of Texas System | Luminous bacteria and methods for the isolation, identification and quantitation of toxicants |
| US6340572B1 (en) * | 1991-04-04 | 2002-01-22 | Board Of Regents, The University Of Texas System | Kit for the isolation, identification and quantitation of toxicants |
| WO1998021347A1 (en) * | 1996-11-15 | 1998-05-22 | E.I. Du Pont De Nemours And Company | A small volume, highly sensitive method for detecting environmental insults |
| WO1999049019A2 (en) * | 1998-03-27 | 1999-09-30 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
| WO2000014267A1 (en) * | 1998-09-09 | 2000-03-16 | Aberdeen University | Biosensor |
| WO2000037665A1 (en) * | 1998-12-22 | 2000-06-29 | The University Of Georgia Research Foundation | Method and system for the continuous synthesis of natural products using immobilized fungal spores |
Non-Patent Citations (1)
| Title |
|---|
| WEITZ HEDDA J ET AL: "Development of a novel, bioluminescence-based, fungal bioassay for toxicity testing.", ENVIRONMENTAL MICROBIOLOGY. ENGLAND JUL 2002, vol. 4, no. 7, July 2002 (2002-07-01), pages 422 - 429, XP001181486, ISSN: 1462-2912 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004076685A2 (en) | 2004-09-10 |
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