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WO1997039326A3 - In vitro fluorescence polarization assay - Google Patents

In vitro fluorescence polarization assay Download PDF

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Publication number
WO1997039326A3
WO1997039326A3 PCT/US1997/006746 US9706746W WO9739326A3 WO 1997039326 A3 WO1997039326 A3 WO 1997039326A3 US 9706746 W US9706746 W US 9706746W WO 9739326 A3 WO9739326 A3 WO 9739326A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
test substance
emission
mixture
pair
Prior art date
Application number
PCT/US1997/006746
Other languages
French (fr)
Other versions
WO1997039326A2 (en
Inventor
Berkley A Lynch
Ian A Macneil
Mark J Zoller
Original Assignee
Ariad Pharma Inc
Berkley A Lynch
Ian A Macneil
Mark J Zoller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ariad Pharma Inc, Berkley A Lynch, Ian A Macneil, Mark J Zoller filed Critical Ariad Pharma Inc
Priority to AU28076/97A priority Critical patent/AU727108B2/en
Priority to JP09530451A priority patent/JP2000512737A/en
Priority to EP97922393A priority patent/EP0898709A2/en
Publication of WO1997039326A2 publication Critical patent/WO1997039326A2/en
Publication of WO1997039326A3 publication Critical patent/WO1997039326A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)

Abstract

An in vitro assay method permits the identification of a test substance which inhibits the mutual association of a pair of proteins. The method includes the steps of providing a pair of proteins capable of mutual association, one of said proteins bearing a covalently linked fluorophore; preparing a mixture containing the two proteins and at least one test substance; irradiating the mixture with polarized light of a suitable wavelength permitting excitation of the fluorophore as indicated by emission of polarized light; measuring the degree of polarization of the emission, and determining the effect of the presence or concentration of the test substance in decreasing the observed emission polarization of a mixture of the two proteins alone. Inhibitory activity of in the test substance correlates with decreased depolarization values.
PCT/US1997/006746 1996-04-18 1997-04-18 In vitro fluorescence polarization assay WO1997039326A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU28076/97A AU727108B2 (en) 1996-04-18 1997-04-18 In vitro fluorescence polarization assay
JP09530451A JP2000512737A (en) 1996-04-18 1997-04-18 In vitro fluorescence polarization assay
EP97922393A EP0898709A2 (en) 1996-04-18 1997-04-18 $i(IN VITRO) FLUORESCENCE POLARIZATION ASSAY

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US1559096P 1996-04-18 1996-04-18
US60/015,590 1996-04-18
US2987096P 1996-11-06 1996-11-06
US60/029,870 1996-11-06

Publications (2)

Publication Number Publication Date
WO1997039326A2 WO1997039326A2 (en) 1997-10-23
WO1997039326A3 true WO1997039326A3 (en) 1997-12-24

Family

ID=26687596

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/006746 WO1997039326A2 (en) 1996-04-18 1997-04-18 In vitro fluorescence polarization assay

Country Status (5)

Country Link
EP (1) EP0898709A2 (en)
JP (1) JP2000512737A (en)
AU (1) AU727108B2 (en)
CA (1) CA2250067A1 (en)
WO (1) WO1997039326A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0938586A4 (en) * 1996-10-28 2002-01-30 Panvera Corp Kinase activity measurement using fluorescence polarization
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
KR20000068915A (en) * 1997-09-05 2000-11-25 마쓰시다 일렉트릭 인더스트리얼 컴패니 리미티드 Fluorescence polarization method
GB9923208D0 (en) * 1999-10-01 1999-12-08 Cambridge Drug Discovery Ltd Assay
UY27304A1 (en) 2001-05-24 2002-12-31 Avanir Pharmaceuticals INHIBITORS OF THE INHIBITOR FACTOR OF MIGRATION OF MACROPHAGES AND METHODS FOR IDENTIFICATION
US7763439B2 (en) 2001-07-02 2010-07-27 Arctic Diagnostics Oy Two-photon absorbing dipyrrometheneboron difluoride dyes and their applications
JP4382474B2 (en) 2001-07-02 2009-12-16 アークティク ダイアグノスティックス オサケユイチア Method for increasing the hydrophilicity of fluorescent label compounds
TW200418829A (en) 2003-02-14 2004-10-01 Avanir Pharmaceutics Inhibitors of macrophage migration inhibitory factor and methods for identifying the same
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2531506A1 (en) 2003-08-22 2005-03-10 Avanir Pharmaceuticals Substituted naphthyridine derivatives as inhibitors of macrophage migration inhibitory factor and their use in the treatment of human diseases
JP4607950B2 (en) * 2004-03-18 2011-01-05 トランス テック ファーマ，インコーポレイテッド Fluorescence polarization assay
US7608407B2 (en) * 2004-11-16 2009-10-27 Boehringer Ingelheim Pharmaceuticals, Inc. Fluorescence polarization assay for determining histidine decarboxylase activity
CA2600175A1 (en) 2005-03-24 2006-03-20 Avanir Pharmaceuticals Thienopyridinone derivatives as macrophage migration inhibitory factor inhibitors
US9164086B2 (en) 2011-10-18 2015-10-20 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques
EP2944958A1 (en) 2014-04-04 2015-11-18 Techno-Path (Distribution) A method of predicting phenotypic instability in a cell
EP3795999A1 (en) * 2014-07-15 2021-03-24 Valitacell Limited A method of determining the abundance of a target molecule in a sample
EP3075844A1 (en) 2015-04-01 2016-10-05 Valitacell Limited A method of determining a compositional or functional characteristic of a cell culture media

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
EP0449269A1 (en) * 1990-03-27 1991-10-02 Roche Diagnostics GmbH Method for detection of phosphorylated tyrosine containing proteins
WO1993003377A1 (en) * 1991-07-31 1993-02-18 Ziltener Hermann J Method for the detection of phosphotyrosine residues
US5352660A (en) * 1991-10-31 1994-10-04 Mount Sinai Hospital Corporation Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system
WO1997010253A1 (en) * 1995-09-15 1997-03-20 Merck & Co., Inc. A high throughput assay using fusion proteins

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
EP0449269A1 (en) * 1990-03-27 1991-10-02 Roche Diagnostics GmbH Method for detection of phosphorylated tyrosine containing proteins
WO1993003377A1 (en) * 1991-07-31 1993-02-18 Ziltener Hermann J Method for the detection of phosphotyrosine residues
US5352660A (en) * 1991-10-31 1994-10-04 Mount Sinai Hospital Corporation Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system
WO1997010253A1 (en) * 1995-09-15 1997-03-20 Merck & Co., Inc. A high throughput assay using fusion proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
B. A. LYNCH ET AL: "A fluorescence polarisation based Src-SH2 binding assay.", ANALYTICAL BIOCHEMISTRY., vol. 247, no. 1, 1997, NEW YORK US, pages 77 - 82, XP002045666 *
P. WU ET AL: "A high-throughput STAT binding assay using fluorescence polatization.", ANALYTICAL BIOCHEMISTRY., vol. 249, no. 1, 1997, NEW YORK US, pages 29 - 36, XP002045667 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes

Also Published As

Publication number Publication date
CA2250067A1 (en) 1997-10-23
JP2000512737A (en) 2000-09-26
AU2807697A (en) 1997-11-07
AU727108B2 (en) 2000-11-30
WO1997039326A2 (en) 1997-10-23
EP0898709A2 (en) 1999-03-03

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