WO2003018834A3 - Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique - Google Patents
Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique Download PDFInfo
- Publication number
- WO2003018834A3 WO2003018834A3 PCT/DE2002/003122 DE0203122W WO03018834A3 WO 2003018834 A3 WO2003018834 A3 WO 2003018834A3 DE 0203122 W DE0203122 W DE 0203122W WO 03018834 A3 WO03018834 A3 WO 03018834A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- acid oligomer
- detection
- hybridization events
- ligands
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne un procédé destiné à la détection d'hybridations d'oligomères d'acide nucléique, ledit procédé comprenant les étapes suivantes: mise à disposition d'une surface modifiée, la modification correspondant à la liaison d'au moins un type d'oligomères d'acide nucléique de ligature; mise à disposition de ligands d'oligomères d'acide nucléique signal; mise à disposition d'un échantillon comprenant des ligands d'oligomères d'acide nucléique; mise en contact d'une quantité définie des ligands d'oligomères d'acide nucléique signal avec la surface modifiée, et mise en contact de l'échantillon et des ligands d'oligomères d'acide nucléique qu'il contient, avec la surface modifiée; détection des ligands d'oligomères d'acide nucléique signal; et comparaison des valeurs obtenues pour la détection des ligands d'oligomères d'acide nucléique signal avec des valeurs de référence.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT02762252T ATE513058T1 (de) | 2001-08-25 | 2002-08-26 | Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen |
| EP02762252A EP1554396B1 (fr) | 2001-08-25 | 2002-08-26 | Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique |
| US10/487,816 US20060228703A1 (en) | 2001-08-25 | 2002-08-26 | Displacement assay for the detection of nucleic acid oligomer hybridization events |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10141691A DE10141691A1 (de) | 2001-08-25 | 2001-08-25 | Verdrängungsassay zur Detektion von Ligat-Ligand-Assoziationsereignissen |
| DE10141691.1 | 2001-08-25 | ||
| PCT/DE2002/001269 WO2003019194A2 (fr) | 2001-08-25 | 2002-04-06 | Essai de deplacement pour la detection d'evenements d'association ligat-ligand |
| DEPCT/DE02/01269 | 2002-04-06 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2003018834A2 WO2003018834A2 (fr) | 2003-03-06 |
| WO2003018834A3 true WO2003018834A3 (fr) | 2003-09-12 |
Family
ID=25750293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/DE2002/003122 WO2003018834A2 (fr) | 2001-08-25 | 2002-08-26 | Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2003018834A2 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005031066A (ja) * | 2003-04-28 | 2005-02-03 | Canon Inc | 固定化核酸プローブの処理方法、核酸の検出方法、核酸濃度分析方法、および核酸検出用キット |
| DE102007044664B4 (de) | 2007-09-18 | 2012-01-05 | Friz Biochem Gesellschaft Für Bioanalytik Mbh | Verdrängungsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen |
| DE102008002267B3 (de) | 2008-06-06 | 2009-11-26 | Friz Biochem Gesellschaft Für Bioanalytik Mbh | Elektrisches Subtrat zum Einsatz als Träger von Biomolekülen |
| DE102009044795B3 (de) | 2009-12-07 | 2011-04-07 | Friz Biochem Gesellschaft Für Bioanalytik Mbh | Kompetitionsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen |
| DE102011056606B3 (de) | 2011-12-19 | 2013-01-03 | Friz Biochem Gesellschaft Für Bioanalytik Mbh | Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993021530A1 (fr) * | 1992-04-10 | 1993-10-28 | Messerschmitt-Bölkow-Blohm Gmbh | Procede et dispositif visant a augmenter la sensibilite et la selectivite pour les dosages immunologiques, les dosages d'interaction molecule-recepteur, adn complementaire - adn et molecule hote - molecule etrangere |
| WO1999051778A1 (fr) * | 1998-04-08 | 1999-10-14 | California Institute Of Technology | Capteur electrochimique utilisant des fractions intercalaires a activite redox |
| WO1999057319A1 (fr) * | 1998-05-06 | 1999-11-11 | Clinical Micro Sensors | Detection electronique d'acides nucleiques a l'aide de monocouches |
| WO2001007665A2 (fr) * | 1999-07-26 | 2001-02-01 | Clinical Micro Sensors, Inc. | Determination de sequences d'acides nucleiques par detection electronique |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69734520D1 (de) | 1996-06-07 | 2005-12-08 | Amersham Biosciences Sv Corp | Nukleinsäurevermittelter elektronentransfer |
| DE19964220C2 (de) | 1998-11-23 | 2003-07-03 | Friz Biochem Gmbh | Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche |
| ATE238436T1 (de) | 1999-01-18 | 2003-05-15 | Friz Biochem Gmbh | Verfahren zur elektrochemischen detektion von nukleinsäure -oligomer- hybridisierungsereignissen |
-
2002
- 2002-08-26 WO PCT/DE2002/003122 patent/WO2003018834A2/fr not_active Application Discontinuation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993021530A1 (fr) * | 1992-04-10 | 1993-10-28 | Messerschmitt-Bölkow-Blohm Gmbh | Procede et dispositif visant a augmenter la sensibilite et la selectivite pour les dosages immunologiques, les dosages d'interaction molecule-recepteur, adn complementaire - adn et molecule hote - molecule etrangere |
| WO1999051778A1 (fr) * | 1998-04-08 | 1999-10-14 | California Institute Of Technology | Capteur electrochimique utilisant des fractions intercalaires a activite redox |
| WO1999057319A1 (fr) * | 1998-05-06 | 1999-11-11 | Clinical Micro Sensors | Detection electronique d'acides nucleiques a l'aide de monocouches |
| WO2001007665A2 (fr) * | 1999-07-26 | 2001-02-01 | Clinical Micro Sensors, Inc. | Determination de sequences d'acides nucleiques par detection electronique |
Non-Patent Citations (3)
| Title |
|---|
| MARRAZZA G ET AL: "Disposable DNA electrochemical sensor for hybridization detection", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 14, 1999, pages 43 - 51, XP002113746, ISSN: 0956-5663 * |
| UMEK R M ET AL: "Bioelectronic detection of point mutations using discrimination of the H63D polymorphism of the Hfe gene as a model.", MOLECULAR DIAGNOSIS: A JOURNAL DEVOTED TO THE UNDERSTANDING OF HUMAN DISEASE THROUGH THE CLINICAL APPLICATION OF MOLECULAR BIOLOGY. UNITED STATES DEC 2000, vol. 5, no. 4, December 2000 (2000-12-01), pages 321 - 328, XP008018952, ISSN: 1084-8592 * |
| WANG J ET AL: "Nucleic-acid immobilization, recognition and detection at chronopotentiometric DNA chips", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 12, no. 7, 1997, pages 587 - 599, XP002113741, ISSN: 0956-5663 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003018834A2 (fr) | 2003-03-06 |
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