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WO2003018834A3 - Displacement assay for the detection of nucleic acid oligomer hybridization events - Google Patents

Displacement assay for the detection of nucleic acid oligomer hybridization events Download PDF

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Publication number
WO2003018834A3
WO2003018834A3 PCT/DE2002/003122 DE0203122W WO03018834A3 WO 2003018834 A3 WO2003018834 A3 WO 2003018834A3 DE 0203122 W DE0203122 W DE 0203122W WO 03018834 A3 WO03018834 A3 WO 03018834A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid oligomer
detection
hybridization events
ligands
Prior art date
Application number
PCT/DE2002/003122
Other languages
German (de)
French (fr)
Other versions
WO2003018834A2 (en
Inventor
Gerhard Hartwich
Peter Frischmann
Ute Haker
Herbert Wieder
Original Assignee
Friz Biochem Gmbh
Gerhard Hartwich
Peter Frischmann
Ute Haker
Herbert Wieder
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10141691A external-priority patent/DE10141691A1/en
Application filed by Friz Biochem Gmbh, Gerhard Hartwich, Peter Frischmann, Ute Haker, Herbert Wieder filed Critical Friz Biochem Gmbh
Priority to EP02762252A priority Critical patent/EP1554396B1/en
Priority to US10/487,816 priority patent/US20060228703A1/en
Priority to AT02762252T priority patent/ATE513058T1/en
Publication of WO2003018834A2 publication Critical patent/WO2003018834A2/en
Publication of WO2003018834A3 publication Critical patent/WO2003018834A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed is a method for the detection of nucleic acid oligomer hybridization events, comprising the following steps: provision of a modified surface, said modification consisting in the binding of at least one type of ligate-nucleic acid oligomer; provision of signal nucleic acid oligomer ligands; provision of a sample with nucleic acid oligomer ligands; a defined amount of signal nucleic acid oligomer ligands is brought into contact with the modified surface and the sample and the nucleci acid oligomer ligands are brought into contact with the modified surface; detection of the signal nucleic acid oligomer ligands and comparing the values obtained during detection of the signal nucleic acid oligomer ligands with reference values.
PCT/DE2002/003122 2001-08-25 2002-08-26 Displacement assay for the detection of nucleic acid oligomer hybridization events WO2003018834A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02762252A EP1554396B1 (en) 2001-08-25 2002-08-26 Displacement assay for the detection of nucleic acid oligomer hybridization events
US10/487,816 US20060228703A1 (en) 2001-08-25 2002-08-26 Displacement assay for the detection of nucleic acid oligomer hybridization events
AT02762252T ATE513058T1 (en) 2001-08-25 2002-08-26 DISPLACEMENT ASSAY FOR DETECTING NUCLEIC ACID OLIGOMER HYBRIDIZATION EVENTS

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10141691A DE10141691A1 (en) 2001-08-25 2001-08-25 Displacement assay for the detection of ligate-ligand association events
DE10141691.1 2001-08-25
DEPCT/DE02/01269 2002-04-06
PCT/DE2002/001269 WO2003019194A2 (en) 2001-08-25 2002-04-06 Displacement assay for detecting ligate-ligand association events

Publications (2)

Publication Number Publication Date
WO2003018834A2 WO2003018834A2 (en) 2003-03-06
WO2003018834A3 true WO2003018834A3 (en) 2003-09-12

Family

ID=25750293

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/003122 WO2003018834A2 (en) 2001-08-25 2002-08-26 Displacement assay for the detection of nucleic acid oligomer hybridization events

Country Status (1)

Country Link
WO (1) WO2003018834A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005031066A (en) * 2003-04-28 2005-02-03 Canon Inc Processing method of immobilized nucleic acid probe, method for detecting nucleic acid, method for analyzing nucleic acid density, and kit for detecting nucleic acid
DE102007044664B4 (en) 2007-09-18 2012-01-05 Friz Biochem Gesellschaft Für Bioanalytik Mbh Displacement Assay for the Detection of Nucleic Acid Oligomer Hybridization Events
DE102008002267B3 (en) 2008-06-06 2009-11-26 Friz Biochem Gesellschaft Für Bioanalytik Mbh Electrical substrate for use as a carrier of biomolecules
DE102009044795B3 (en) 2009-12-07 2011-04-07 Friz Biochem Gesellschaft Für Bioanalytik Mbh Competition assay for the detection of nucleic acid oligomer hybridization events
DE102011056606B3 (en) 2011-12-19 2013-01-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh A method for the electrochemical detection of nucleic acid oligomer hybridization events

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021530A1 (en) * 1992-04-10 1993-10-28 Messerschmitt-Bölkow-Blohm Gmbh PROCESS AND DEVICE FOR INCREASING THE SENSITIVITY AND SELECTIVITY OF IMMUNO-ASSAYS, MOLECULE-RECEPTOR, DNA-COMPLEMENTARY DNA and FOREIGN MOLECULE-HOST MOLECULE INTERACTION ASSAYS
WO1999051778A1 (en) * 1998-04-08 1999-10-14 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
WO1999057319A1 (en) * 1998-05-06 1999-11-11 Clinical Micro Sensors Electronic detection of nucleic acids using monolayers
WO2001007665A2 (en) * 1999-07-26 2001-02-01 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000511904A (en) 1996-06-07 2000-09-12 カリフォルニア インスティテュート オブ テクノロジー Electron transfer by nucleic acids
DE19964220C2 (en) 1998-11-23 2003-07-03 Friz Biochem Gmbh Modified nucleic acid oligomer, useful for sequencing by hybridization, is substituted by redox agent to allow electrical detection of hybridization
ATE238436T1 (en) 1999-01-18 2003-05-15 Friz Biochem Gmbh METHOD FOR ELECTROCHEMICAL DETECTION OF NUCLEIC ACID OLIGOMER HYBRIDIZATION EVENTS

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021530A1 (en) * 1992-04-10 1993-10-28 Messerschmitt-Bölkow-Blohm Gmbh PROCESS AND DEVICE FOR INCREASING THE SENSITIVITY AND SELECTIVITY OF IMMUNO-ASSAYS, MOLECULE-RECEPTOR, DNA-COMPLEMENTARY DNA and FOREIGN MOLECULE-HOST MOLECULE INTERACTION ASSAYS
WO1999051778A1 (en) * 1998-04-08 1999-10-14 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
WO1999057319A1 (en) * 1998-05-06 1999-11-11 Clinical Micro Sensors Electronic detection of nucleic acids using monolayers
WO2001007665A2 (en) * 1999-07-26 2001-02-01 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MARRAZZA G ET AL: "Disposable DNA electrochemical sensor for hybridization detection", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 14, 1999, pages 43 - 51, XP002113746, ISSN: 0956-5663 *
UMEK R M ET AL: "Bioelectronic detection of point mutations using discrimination of the H63D polymorphism of the Hfe gene as a model.", MOLECULAR DIAGNOSIS: A JOURNAL DEVOTED TO THE UNDERSTANDING OF HUMAN DISEASE THROUGH THE CLINICAL APPLICATION OF MOLECULAR BIOLOGY. UNITED STATES DEC 2000, vol. 5, no. 4, December 2000 (2000-12-01), pages 321 - 328, XP008018952, ISSN: 1084-8592 *
WANG J ET AL: "Nucleic-acid immobilization, recognition and detection at chronopotentiometric DNA chips", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 12, no. 7, 1997, pages 587 - 599, XP002113741, ISSN: 0956-5663 *

Also Published As

Publication number Publication date
WO2003018834A2 (en) 2003-03-06

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