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WO2003012389A3 - Procede pour analyser des macromolecules, dispositif d'analyse et procede pour produire un dispositif d'analyse - Google Patents

Procede pour analyser des macromolecules, dispositif d'analyse et procede pour produire un dispositif d'analyse Download PDF

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Publication number
WO2003012389A3
WO2003012389A3 PCT/EP2002/007644 EP0207644W WO03012389A3 WO 2003012389 A3 WO2003012389 A3 WO 2003012389A3 EP 0207644 W EP0207644 W EP 0207644W WO 03012389 A3 WO03012389 A3 WO 03012389A3
Authority
WO
WIPO (PCT)
Prior art keywords
drop
macromolecules
liquid
spots
microarray
Prior art date
Application number
PCT/EP2002/007644
Other languages
German (de)
English (en)
Other versions
WO2003012389A2 (fr
Inventor
Juergen Scriba
Christoph Gauer
Original Assignee
Advalytix Ag
Juergen Scriba
Christoph Gauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advalytix Ag, Juergen Scriba, Christoph Gauer filed Critical Advalytix Ag
Priority to EP02764659A priority Critical patent/EP1412533A2/fr
Priority to AU2002328867A priority patent/AU2002328867A1/en
Publication of WO2003012389A2 publication Critical patent/WO2003012389A2/fr
Publication of WO2003012389A3 publication Critical patent/WO2003012389A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00533—Sheets essentially rectangular
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0493—Specific techniques used
    • B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)

Abstract

La présente invention concerne un procédé pour analyser des macromolécules à l'aide d'un microréseau constitué de plusieurs taches situées selon une disposition connue. Ces taches comprennent une pluralité de premières macromolécules au moins partiellement différentes. Ledit microréseau se trouve sur une surface solide, de préférence sur une puce. Selon ce procédé, au moins une goutte de liquide présentant des macromolécules est appliquée sur ladite surface solide. La quantité de liquide est si faible que seulement une tache ou un groupe de quelques taches du microréseau vient en contact avec la goutte de liquide. Après un intervalle de temps prédéfini, la goutte de liquide est déplacée vers une tache suivante ou vers un autre groupe de quelques taches, à l'aide d'un report d'impulsion d'une onde sonore de surface. On étudie ensuite si une réaction avec la matière de la goutte de liquide a eu lieu dans les taches qui sont venues en contact avec la goutte de liquide. La présente invention concerne également un procédé pour produire un dispositif d'analyse pour macromolécules. Selon ce procédé, au moins une goutte de liquide présentant au moins un type de macromolécule est appliquée sur une surface solide, de préférence sur une puce. Ces macromolécules présentent un groupe actif qui est activé lors de la modification d'un paramètre extérieur correspondant, afin de permettre une liaison à la surface ou à des molécules déjà liées. La goutte est déplacée à un endroit souhaité sur la surface, à l'aide du report d'impulsion d'une ou de plusieurs ondes sonores de surface. Le paramètre extérieur est modifié de manière homogène et non locale de façon à permettre une liaison des macromolécules, formant la tache d'un microréseau dans laquelle des macromolécules de la goutte de liquide sont liées à la surface. En outre, cette invention concerne un dispositif d'analyse permettant de mettre en oeuvre le procédé d'analyse de la présente invention.
PCT/EP2002/007644 2001-07-24 2002-07-09 Procede pour analyser des macromolecules, dispositif d'analyse et procede pour produire un dispositif d'analyse WO2003012389A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02764659A EP1412533A2 (fr) 2001-07-24 2002-07-09 Procede pour analyser des macromolecules, dispositif d'analyse et procede pour produire un dispositif d'analyse
AU2002328867A AU2002328867A1 (en) 2001-07-24 2002-07-09 Method for analysing macromolecules, analysis device and a method for producing an analysis device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10136008A DE10136008B4 (de) 2001-07-24 2001-07-24 Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung
DE10136008.8 2001-07-24

Publications (2)

Publication Number Publication Date
WO2003012389A2 WO2003012389A2 (fr) 2003-02-13
WO2003012389A3 true WO2003012389A3 (fr) 2003-07-17

Family

ID=7692898

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/007644 WO2003012389A2 (fr) 2001-07-24 2002-07-09 Procede pour analyser des macromolecules, dispositif d'analyse et procede pour produire un dispositif d'analyse

Country Status (4)

Country Link
EP (1) EP1412533A2 (fr)
AU (1) AU2002328867A1 (fr)
DE (1) DE10136008B4 (fr)
WO (1) WO2003012389A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10328624A1 (de) * 2003-06-25 2005-01-20 Advalytix Ag Verfahren zur Hybridisierung
DE102004037348A1 (de) * 2004-08-02 2006-03-16 Infineon Technologies Ag Fluid-Transport-Vorrichtung, Sensor-Anordnung, Fluid-Misch-Vorrichtung und Verfahren zum Herstellen einer Fluid-Transport-Vorrichtung
DE102004051394B4 (de) * 2004-10-21 2006-08-17 Advalytix Ag Verfahren zur Bewegung von kleinen Flüssigkeitsmengen in Mikrokanälen und Mikrokanalsystem
DE102005000835B3 (de) 2005-01-05 2006-09-07 Advalytix Ag Verfahren und Vorrichtung zur Dosierung kleiner Flüssigkeitsmengen
US8062904B2 (en) 2005-01-05 2011-11-22 Beckman Coulter, Inc. Method and device for dosing and mixing small amounts of liquid
DE102005056639A1 (de) * 2005-11-28 2007-06-06 Advalytix Ag Verfahren, Vorrichtung und Kit zur Untersuchung von Makromolekülen in einer Probe
DE102006056694B4 (de) * 2006-11-30 2010-08-05 Advalytix Ag Verfahren zum Durchführen einer enzymatischen Reaktion
WO2008080531A2 (fr) * 2007-01-05 2008-07-10 Advalytix Ag Procédé, dispositif et kit pour l'examen d'un échantillon de liquide
EP2194381B1 (fr) * 2008-12-03 2015-12-02 Roche Diagnostics GmbH Elément de test doté d'une zone de contrôle et de calibrage combinée
EP2809428A4 (fr) * 2012-01-31 2015-11-04 Penn State Res Found Manipulation microfluidique et tri de particules à l'aide d'une onde acoustique de surface stationnaire et synchronisable

Citations (4)

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Publication number Priority date Publication date Assignee Title
WO1994027719A1 (fr) * 1993-05-27 1994-12-08 Protogene Laboratories, Inc. Procede et appareil pour realiser une serie de reactions chimiques sur une surface de support
US5674742A (en) * 1992-08-31 1997-10-07 The Regents Of The University Of California Microfabricated reactor
US6010316A (en) * 1996-01-16 2000-01-04 The Board Of Trustees Of The Leland Stanford Junior University Acoustic micropump
DE19836110A1 (de) * 1998-08-10 2000-02-24 Biotul Bio Instr Gmbh Vorrichtung und Verfahren zur grenzflächennahen Mischung von Proben in Biosensorsystemen

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WO2000012123A2 (fr) * 1998-08-28 2000-03-09 Febit Ferrarius Biotechnology Gmbh Procede et dispositif de mesure pour la determination d'une pluralite d'analytes dans un echantillon
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US5674742A (en) * 1992-08-31 1997-10-07 The Regents Of The University Of California Microfabricated reactor
WO1994027719A1 (fr) * 1993-05-27 1994-12-08 Protogene Laboratories, Inc. Procede et appareil pour realiser une serie de reactions chimiques sur une surface de support
US6010316A (en) * 1996-01-16 2000-01-04 The Board Of Trustees Of The Leland Stanford Junior University Acoustic micropump
DE19836110A1 (de) * 1998-08-10 2000-02-24 Biotul Bio Instr Gmbh Vorrichtung und Verfahren zur grenzflächennahen Mischung von Proben in Biosensorsystemen

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WIXFORTH A., SCRIBA J.: "Nanopumpen für das Chiplabor", GIT LABOR-FACHZEITSCHRIFT, vol. 05, 2002, pages 594, XP009009065 *

Also Published As

Publication number Publication date
WO2003012389A2 (fr) 2003-02-13
EP1412533A2 (fr) 2004-04-28
DE10136008B4 (de) 2005-03-31
AU2002328867A1 (en) 2003-02-17
DE10136008A1 (de) 2003-02-20

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