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WO2003068790A2 - Macrolide antibacterial compounds - Google Patents

Macrolide antibacterial compounds Download PDF

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Publication number
WO2003068790A2
WO2003068790A2 PCT/US2003/004117 US0304117W WO03068790A2 WO 2003068790 A2 WO2003068790 A2 WO 2003068790A2 US 0304117 W US0304117 W US 0304117W WO 03068790 A2 WO03068790 A2 WO 03068790A2
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WO
WIPO (PCT)
Prior art keywords
ethyl
hydrogen
trideoxy
trioxo
xylo
Prior art date
Application number
PCT/US2003/004117
Other languages
French (fr)
Other versions
WO2003068790A3 (en
Inventor
Zhenkun Ma
Richard Clark
Stevan Djuric
Sanyi Wang
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/075,011 external-priority patent/US20030171308A1/en
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Priority to CA002476223A priority Critical patent/CA2476223A1/en
Priority to MXPA04007883A priority patent/MXPA04007883A/en
Priority to JP2003567916A priority patent/JP2005522453A/en
Priority to EP03707854A priority patent/EP1474431A2/en
Publication of WO2003068790A2 publication Critical patent/WO2003068790A2/en
Publication of WO2003068790A3 publication Critical patent/WO2003068790A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
    • C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • TECHNICAL FIELD This invention is directed to compounds with antibacterial activity, processes for making the compounds and intermediates used in the processes, compositions containing the compounds, and methods for prophylaxis or treatment of bacterial infections using the compounds .
  • a first embodiment of this invention is directed to compounds, and salts, prodrugs, and salts of prodrugs thereof, having antibacterial activity, the compounds having formula (I)
  • a and B together are one- to seven-membered alkylene or two- to seven-membered heteroalkylene, and D 1 and E1 are hydrogen; or D1 and E1 together are one- to seven-membered alkylene or two- to seven-membered heteroalkylene, and A and B are hydrogen;
  • X is hydrogen or fluoride
  • Y is arylene or heteroarylene
  • W is hydrogen aryl, heteroaryl, or heterocyclyl
  • R is alkyl, aryl, heteroaryl, or heterocyclyl
  • R 2 and R3 are independently hydrogen or alkyl
  • R 2 and R3 together are 3- to 7-membered alkylene or 3- to 7-membered heteroalkylene;
  • R is hydrogen or alkyl;
  • R and R are independently hydrogen or alkyl;
  • R30 is alkyl or alkyl substituted with a substituent selected from the group consisti ⁇ ng of halo and -OR45; R and R are independently selected alkyl; R is phenyl, naphthyl, furyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, 1, 2, 3-oxadiazolyl, 1, 2, 3-thiadiazolyl, 1, 3, -thiadiazolyl, 1, 2, 3-triazolyl, tetrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyrrolidinyl, inidazolidinyl, piperidinyl, piperazinyl, morpholinyl, or thiomorpholinyl, each of which is unsubstituted or substituted with one, two, or three substituents independently selected from the group consisting of
  • a second embodiment of this invention is directed to processes for making the compounds of the first embodiment.
  • a third embodiment of this invention is directed to intermediates which are useful in the second embodiment.
  • a fourth embodiment of this invention is directed to compositions comprising a therapeutically effective amount of a compound of the first embodiment.
  • a fifth embodiment of this invention is directed to methods for prophylaxis or treatment of bacterial infections in a fish or a mammal comprising administering thereto a therapeutically effective amount of a compound of the first ⁇ embodiment .
  • the beneficiary of prophylaxis or treatment of bacterial infections is a mammal.
  • the beneficiary of prophylaxis or treatment of bacterial infections is a human.
  • the compounds of this invention comprise both fixed and variable moieties, the latter of which are identified by a capital letter and accompanying numerical or alphabetical superscript, in which the term “alkenyl” means a monovalent, straight or branched hydrocarbon, having two to eight carbon atoms and at least one carbon-carbon double bond, attached through a carbon atom; the term “alkenylene” means a divalent, straight or branched hydrocarbon, having two to eight carbon atoms and one carbon-carbon double bond, attached through carbon atoms ; the term “alkynyl” means a monovalent, straight or branched hydrocarbon, having two to eight carbon atoms and at least one carbon-carbon triple bond, attached through a carbon atom; the term “alkynylene” means a divalent, straight or branched hydrocarbon, having two to eight carbon atoms and one carbon-carbon triple bond, attached through carbon atoms; the term “alkyl” means a monovalent, saturated, straight
  • Preferred A I, Bl, Dl, and E1 moieties are hydrogen.
  • a preferred X moiety is hydrogen.
  • a preferred M moiety is C ⁇ C.
  • Preferred Y moieties are 1, 3-phenylene, 1, -phenylene, and 2, 5-thienylene .
  • Preferred moieties are phenyl, 3-fluorophenyl, 4- (1, 2, 3-thiadiazol-4-yl) phenyl, phenyl fused with another phenyl (naphthyl) , pyridyl, and pyridyl fused with phenyl (quinolinyl) .
  • Preferred R moieties are hydrogen and alkyl.
  • a preferred R moiety is hydrogen.
  • R A moi ⁇ ety i ⁇ s hydrogen A preferred R A moi ⁇ ety i ⁇ s hydrogen. These preferred variable moieties combine with the parent moiety to form a preferred first embodiment of this invention, the preferred first embodiment comprising compounds, and salts, prodrugs, and salts of prodrugs thereof, having formula (I)
  • variable moieties also combine to form still yet another preferred first embodiment of this invention, the preferred first embodiment comprising compounds, and salts, prodrugs, and salts of prodrugs thereof, which are (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6,8, 12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( (phenylamino) methyl) thien-2-yl) prop-2-ynyl) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2.
  • the compounds of this invention comprise asymmetrically substituted carbon atoms in the R or S configuration.
  • Asymmetric carbon atoms with equimolar amounts of R and S configurations are racemic. Atoms with an excess of one configuration over the other are assigned the configuration in the higher amount, preferably an excess of about 85%-90%, more preferably an excess of about 95%-99%, and still more preferably an excess greater than about 99%.
  • the compounds of this invention may also comprise carbon-carbon double bonds as being in the Z or E configuration, in which the term “Z” represents the larger two of the four substituents disposed on same side of a carbon-carbon double bond and the term “E” represents the larger two of the four substituents disposed on opposit'e sides of a carbon-carbon double bond.
  • the compounds may also exist as an equilibrium mixture comprising Z or E configurations .
  • the compounds of this invention containing hydroxyl, amino, or carboxylic acids may have attached thereto prodrug-forming moieties.
  • the prodrug-forming moieties are removed by metabolic processes and release the compounds having the freed hydroxyl, amino, or carboxylic acid in vivo.
  • Prodrugs are useful for adjusting such pharmacokinetic properties of the compounds as solubility and/or hydrophobicity, absorption in the gastrointestinal tract, bioavailabil ' ity, tissue penetration, and rate of clearance .
  • the compounds of this invention may be prepared by synthetic processes or metabolic processes. Metabolic processes include those processes occurring in vitro in vivo .
  • the compounds of this invention may exist as acid addition salts, basic addition salts, or zwitterions .
  • Salts of the compounds are prepared during their isolation or following their purification.
  • Acid addition salts of the compounds are those derived from the reaction of the compounds with an acid.
  • Excipients include encapsulating materials or formulation additives such as absorption accelerators, antioxidants, binders, buffers, coating agents, coloring agents, diluents, disintegrating agents, emulsifiers, extenders, fillers, flavoring agents, humectants, lubricants, perfumes, preservatives, propellants, releasing agents, sterilizing agents, t sweeteners, solubilizers, wetting agents, and mixtures thereof.
  • Excipients for orally administered compounds in solid dosage forms include agar, alginic acid, cocoa butter, gelatin, isotonic saline, malt, powdered tragacanth,
  • Ringer's solution talc, water, aluminum hydroxide, magnesium hydroxide, sodium and potassium phosphate salts, cellulose, cellulose acetate, ethyl cellulose, sodium carboxymethyl cellulose, ethyl laureate, ethyl oleate, magnesium stearate, sodium lauryl sulfate, castor oil, corn oil, cottonseed oil, germ oil, groundnut oil, olive oil, peanut oil, safflower oil, sesame oil, soybean oil, benzyl alcohol, benzyl benzoate, 1,3-butylene glycol, ethanol, ethyl acetate, ethyl carbonate, glycerol, isopropanol, propylene glycol, tetrahydrofurfuryl alcohol, corn starch, potato starch, lactose, glucose sucrose, and mixtures thereof.
  • Excipients for ophthalmically and orally administered compounds in liquid dosage forms include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, cottonseed oil, groundnut oil, corn oil, germ oil, olive oil, castor oil, sesame oil, glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols, fatty acid esters of sorbitan, and mixtures thereof.
  • Excipients for osmotically administered compounds include water, ethanol, isopropanol, chlorofluorohydrocarbons, and mixtures thereof.
  • Excipients for parenterally administered compounds include water, 1, 3-butanediol, Ringer's solution, U.S. P. or isotonic sodium chloride solution, oleic acid, castor oil, corn oil, cottonseed oil, germ oil, groundnut oil, olive oil, peanut oil, safflower oil, sesame oil, soybean oil, liposomes, and mixtures thereof.
  • Excipients for rectally and vaginally administered compounds include cocoa butter, polyethylene glycol, wax, and mixtures thereof.
  • the compounds of this invention may be administered parenterally (subcutaneously, intravenously, intramuscularly, and intrasternally) , orally, osmotically, ophthalmically, rectally, topically, and vaginally.
  • Orally administered compounds in solid dosage forms may be administered as capsules, dragees, granules, pills, powders, and tablets.
  • Ophthalmically and orally administered compounds in liquid dosage forms may be administered as elixirs, emulsions, microemulsions, solutions, suspensions, and syrups.
  • Osmotically and topically administered compounds may be administered as creams, gels, inhalants, lotions, ointments, pastes, powders, solutions, and sprays.
  • Parenterally administered compounds may be administered as aqueous or oleaginous solutions or aqueous or oleaginous suspensions, the latter of which contains crystalline, amorphous, or otherwise insoluble forms of the compounds. Rectally and vaginally administered compounds may be administered as creams, gels, lotions, ointments, and pastes .
  • Dosage forms for the compounds of this invention depend on the species being treated, the disorder being treated and the severity thereof, the composition comprising the compounds, the time of administration, the route of administration, the duration of treatment, the potency of the compounds, and the rate of excretion of the compounds.
  • the daily therapeutically effective amount of the compounds administered to a patient in single or divided doses range from about 0.1 to about 200 mg/kg body weight, preferably from about 0.25 to about 100 mg/kg body weight.
  • Single dose compositions contain these amounts of the compounds or combinations of submultiples thereof.
  • Compounds having formula (1) may be converted to p compounds having formula (2), in which R is acetyl (CH 3 C(0)), benzoyl (CeH 5 C(0)), or trimethylsilyl, by reacting the former, a hydroxyl protecting reagent, a first base, and, optionally, N,N-dimethylaminopyridine .
  • Hydroxyl protecting reagents include benzoic anhydride, acetic anhydride, benzoyl chloride, acetyl chloride, and trimethylsilyl chloride.
  • First bases include triethylamine, diisopropylethylamine, pyridine, and lutidine.
  • the reaction is typically conducted at about 0 °C to 60 °C, over about 4 to 24 hours, in solvents such as dichloromethane, chloroform, THF, DME, and tert-butyl methylether.
  • Compounds having formula (2) may be converted to compounds having formula (3) by reacting the former, carbonyldiimidazole, a second base, and, optionally, N,N- dimethylaminopyridine .
  • Second bases include 1,8- diazabicyclo- [5.4.0] undec-7-ene, lithium bis (trimethylsilyl) amide, sodium bis (trimethylsilyl) amide, and potassium bis (trimethylsilyl) amide .
  • the reaction is typically conducted at about 25 °C, over about 6 to 24 hours, in solvents such as THF, DMF, 1,4-dioxane, and
  • Compounds having formula (3) may be converted to compounds having formula (5) by (a) reacting the former and a compound having formula (4)
  • First acids include hydrochloric acid, triflic acid, para-toluenesulfonic acid, and trifluoroacetic acid.
  • Step (a) is typically conducted at about 25 °C, over about 24 hours to 72 hours, in solvents such as acetonitrile, DMF, water, and mixtures thereof.
  • Step (b) is typically conducted at about 70 °C to 100 °C, over about 12 hours to about 24 hours, in solvents such as benzene, toluene, xylene, and mixtures thereof.
  • Compounds having formula (5) may be converted to compounds having formula (6) by reacting the former and a second acid.
  • Second acids include hydrochloric acid, triflic acid, para-toluenesulfonic acid, and trifluoroacetic acid.
  • the reaction is typically conducted at about 60 °C, over about 12 to 24 hours, in solvents such as ethanol, acetone, THF, water, and mixtures thereof.
  • Compounds having formula (6) may be converted to compounds having formula (7) by reacting the former, a first oxidizing agent, and, optionally, a first additive.
  • First oxidizing agents include dimethylsulfide/N- chlorosuccinimide, dimethylsulfoxide/1- (3- dimethylaminopropyl) -3-ethylcarbodiimide, and dimethylsulfoxide/oxalyl chloride.
  • First additives include phosphoric acid, pyridinium trifluoroacetate, silica gel, triethylamine, and pyridine. The reaction is typically conducted at about -10 °C to 25 °C, over about 3 to 24 hours, in solvents such as THF, DMSO, and dichloromethane.
  • Compounds having formula (8) may be converted to compounds having formula (9) by reacting the former, a fluorinating agent, and, optionally, a third base.
  • Fluorinating agents include 3, 5-dichloro-l-fluoropyridinium tetrafluoroborate, N-fluorobenzenesulfonimide, 3, 5-dichloro- l-fluoropyridinium triflate, N-fluoro-N-methyl-para- toluenesulfonamide, N-fluoropyridinium triflate, or N-fluoroperfluoropiperidine .
  • Third bases include sodium hydride, potassium hydride, lithium diisopropylamide, triethylamine, and N,N-diisopropylethylamine.
  • the reactions are typically conducted at about -78 °C to 0 °C, over about 2 to 24 hours, in solvents such as DMF, THF, and diethyl ether.
  • Coupling catalysts include dichlorobis (triphenylphosphine) palladium (II) , tris (dibenzylideneacetone) dipalladium(O) , tetrakis (triphenylphosphine) palladium (0) , and dichlorobis (triphenylphosphine) nickel (II) .
  • Second additives include triphenylphosphine, triphenylarsine, 1,2- bis (diphenylphosphino) butane, 1, 2- bis (diphenylphosphino) ethane, copper (I) iodide, and mixtures thereof.
  • the reactions are typically conducted at about 50 °C to 80 °C, over about 12 to 48 hours, in solvents such as acetonitrile, DME, THF, and mixtures thereof.
  • Compounds having formula (12) may be converted to compounds having formula (13) by reacting the former and a second oxidizing agent.
  • Second oxidizing agents include molecular oxygen, potassium permanganate, sodium chlorite, and silver oxide. The reactions are typically conducted at about 0 °C to 35 °C, over about 1 to 48 hours, in solvents such as acetonitrile, DME, THF, tert-butanol, water, and mixtures thereof.
  • Q is absent, 0, or alkenylene; and in which Q , z, and Q may combine with the fixed moieties between Y 1 and W1 to form moieties represented by L , and the dilute first acid.
  • the reaction is typically conducted at about 25 °C, over about 24 hours to 72 hours, in solvents such as acetonitrile, DMF, water, and mixtures thereof.
  • Compounds having formula (I) -a may be converted to compounds having formula (I)-b by reacting the former and a reducing agent.
  • Reducing agents include sodium cyanoborohydride and sodium borohydride. The reaction is typically conducted at about 0 °C to 25 °C, over about 24 hours to 72 hours, in solvents such as methanol, ethanol, iso-propyl alcohol, acetonitrile, DMF, water, and mixtures thereof .
  • Dehydrating agents include 1,3- dicyclohexylcarbodiimide and 1- (3-dimethylaminopropyl) -3- ethylcarbodiimide .
  • the reaction is typically conducted at about 0 °C to 25 °C, over about 24 hours to 72 hours, in solvents such as acetonitrile, DMF, water, and dichloromethane .
  • Compounds having formula (13) may be converted to compounds having formula (I)-d by (a) reacting the former and diphenylphosphoryl azide to form a compound having formula (16) and (b) reacting the product of step (a) with a compound having formula (17)
  • Step (a) is typically conducted at about 25 °C to 80 °C, over about 1 to 8 hours, in solvents such as benzene, toluene, and acetonitrile.
  • Step (b) is typically conducted at about 25 °C to 100 °C, over about 1 to 24 hours, in solvents such as benzene, toluene, and acetonitrile, DMF, and DME .
  • Compounds having formula (I)-h may be converted to compounds having formula (I)-i by reacting the former, hydrogen gas, a hydrogenation catalyst, and, optionally, quinoline.
  • Hydrogenation catalysts include Lindlar catalyst and palladium on barium sulfate. The reaction is typically conducted at 25 °C, over about 1 to 6 hours, in solvents such as methanol, ethanol, propanol, butanol, iso-propanol, tert-butanol, acetonitrile, THF, ethyl acetate, and mixtures thereof.
  • Compounds having formula (10) may be converted to compounds having formula (24) by reacting the former and compounds having formula (23) ⁇ B(V 1 ) 3 ,
  • each V 1 is independently hydrogen, alkyl, OH, or OR 45 .
  • the reaction is typically conducted at about -20 °C to 25 °C, over about 1 to 6 hours, in solvents such as THF, DME, and diethyl ether.
  • Compounds having formula (24) may be converted to compounds having formula (I)-j by reacting the former, compounds having formula (25)
  • Coupling catalysts include dichlorobis (triphenylphosphine) palladium (II) , tris (dibenzylideneacetone) dipalladium(O) , tetrakis (triphenylphosphine) palladium(0) , and dichlorobis (triphenylphosphine) nickel (II) .
  • Fourth bases include sodium carbonate, sodium bicarbonate, potassium carbonate, cesium carbonate, triethylamine, and diisopropylethylamine . The reaction is typically conducted at about 50 °C to 80 °C over about 12 to 48 hours, in solvents such as acetonitrile, THF, DMF, and DME.
  • Compounds having formula (I) , m whi .ch RA is RP, and RP is trimethylsilyl, may be converted to compounds having formula (I) , in which R A is hydrogen, by reacting the former and a fluoride-donating agent.
  • Fluoride-donating agents include tetrabutylammonium fluoride, polymer-bound ammonium fluoride, tetrabutylammonium fluoride, pyridine -HF, and triethylamine -trihydrofluoride .
  • the reaction is typically conducted at about 0 °C to 50 °C, over about 1 to 24 hours, in solvents such as THF and 1,4-dioxane.
  • solvents such as THF and 1,4-dioxane.
  • EXAMPLE 1 This example was prepared as described in commonly owned US 6,075,133, EXAMPLE 246, step 246c.
  • EXAMPLE 2 A solution of EXAMPLE 1 (10 g) , 4-dimethyl- aminopyridine (50 mg) , benzoic anhydride (7.02 g) , and triethylamine (3.8 mL) in dichloromethane (70 mL) at 15 °C was stirred for 20 minutes at 15 °C and at ambient temperature for 7 hours, diluted with ethyl acetate, washed with 5% sodium carbonate, water, and brine, and dried (Na S0 4 ) , filtered, and concentrated.
  • EXAMPLE 3 A solution of EXAMPLE 2 (9.80 g) , carbonyldiimidazole (4.05 g) , and 4-dimethylaminopyridine (122 mg) in THF (45 mL) and DMF (13 mL) was treated with 1, 8-diazabicyclo-
  • EXAMPLE 5 A solution of EXAMPLE 4 (5.95 g) and 2M HCl (5 mL) in ethanol (5 mL) was heated to 55 °C for 12 hours and concentrated. The concentrate was dissolved in water, washed with diethyl ether, treated with saturated ammonium hydroxide and extracted with dichloromethane; and the extract was concentrated.
  • EXAMPLE 6 A solution of N-chlorosuccinimide (5.46 g) in dichloromethane (200 mL) at -10 °C was treated with dimethyl sulfide (3.50 mL) , stirred for 10 minutes, treated with a solution of EXAMPLE 5 (20.9 g) in dichloromethane (90 mL) over 30 minutes, stirred for 1 hour, treated with triethylamine (3.79 mL) , stirred for 90 minutes, washed with 5% sodium bicarbonate and brine, and dried (NaS0 4 ) , filtered, and concentrated.
  • EXAMPLE 7 A solution of EXAMPLE 6 (14 g) in methanol (100 mL) was heated at 60 °C for 16 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 97:2:1 dichloro ethane/methanol/concentrated ammonium hydroxide .
  • EXAMPLE 8 A mixture of EXAMPLE 7 (3.75 g) , 5-bromothiophene-2- carboxaldehyde (1.55 g) , dichlorobis (triphenylphosphine) - palladium (II) (76 mg) , and copper(I) iodide (10.2 mg) in acetonitrile (60 mL) and triethylamine (20 mL) was heated at 75 °C for 14 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 97:2:1 dichloromethane/methanol/concentrated ammonium hydroxide.
  • EXAMPLE 9 A mixture of EXAMPLE 8 (350 mg) , aniline (82.9 ⁇ L) , and acetic acid (77.8 ⁇ L) in methanol (8 mL) was stirred at ambient temperature for 2 hours, treated with sodium cyanoborohydride (57 mg) , heated at 60 °C for 1 hour, treated with dichloromethane, washed with 5% sodium bicarbonate and brine, and dried (Na 2 S0 4 ) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1:1 dichloromethane/methanol/ concentrated ammonium hydroxide.
  • EXAMPLE 14 This example was prepared by substituting O- (2-phenoxy- ethyl) hydroxylamine for O-naphthalen-1-ylmethylhydroxylamine hydrochloride in EXAMPLE 12.
  • EXAMPLE 15 A mixture of EXAMPLE 7 (350 mg) , N-benzyl-N- ( (5- bromothien-2-yl) ethyl) amine (172 mg) , dichlorobis- (triphenylphosphine) palladium (II) (7.4 mg) , triethylamine (2 mL) and copper (I) iodide (2 mg) in acetonitrile (6 mL) was heated at 80 °C for 20 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1.5:0.5 dichloromethane/methanol/concentrated ammonium hydroxide.
  • EXAMPLE 16 A mixture of EXAMPLE 8 (200 mg) , 2-hydrazinopyridine (42.3 mg) , and magnesium sulfate (50 mg) in THF was heated at 66 °C for 16 hours, and filtered. The filtrate was treated with ethyl acetate, washed with water and brine, dried (Na S0 4 ) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 97.5:2:0.5 dichloromethane/methanol/concentrated ammonium hydroxide.
  • EXAMPLE 17 This example was prepared by substituting pyridine-2- carbohydrazide for 2-hydrazinopyridine in EXAMPLE 16.
  • EXAMPLE 18 This example was prepared by substituting O-quinol-3- ylmethylhydroxylamine for O-naphthalen-1- ylmethylhydroxylamine hydrochloride in EXAMPLE 12.
  • EXAMPLE 19 This example was prepared by substituting N-((5- bromothien-2-yl) methyl) -N- (2-phenylethyl) amine for N-benzyl- N- ( (5-bromothien-2-yl) methyl) amine in EXAMPLE 15.
  • EXAMPLE 20 This example was prepared by substituting N-((5- bromothien-2-yl) methyl) -N- (3-phenylpropyl) amine for N- benzyl-N- ( (5-bromothien-2-yl) methyl) amine in EXAMPLE 15.
  • EXAMPLE 21 A mixture of EXAMPLE 7 (350 mg) , 2- (3- bro ophenoxy) pyridine (251 mg) , dichlorobis (triphenyl- phosphine) palladiu (II) (11 mg) , and copper (I) iodide (1 mg) , and triethylamine (2 mL) in acetonitrile (6 mL) was heated at 70 °C for 20 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1.5:0.5 dichloromethane/methanol/concentrated ammonium hydroxide.
  • EXAMPLE 22 This example was prepared by substituting 5-bromo-N- (3- fluorophenyl) thiophene-2-carboxamide for 2- (3- bromophenoxy) pyridine in EXAMPLE 21.
  • EXAMPLE 23 This example was prepared by substituting 5-bromo-N- (3- fluorophenyl) -N-methylthiophene-2-carboxamide for 2- (3- bromophenoxy) pyridine in EXAMPLE 21.
  • EXAMPLE 26 A solution of EXAMPLE 25, (300 mg) , 2-ethynylpyridine (41.7 mg) , dichlorobis (triphenylphosphine) palladium(II) (4.5 mg) , copper (I) iodide (0.6 mg) and triethylamine (1 mL) in acetonitrile (3 mL) was heated at 75 °C for 14 hours and concentrated .
  • EXAMPLE 26A A solution of EXAMPLE 26 in methanol (10 mL) was heated at 60 °C for 14 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1:1 dichloromethane/methanol/concentrated ammonium hydroxide.
  • EXAMPLE 28 This example was prepared by substituting 5-bromo-N- pyridin-3-ylthiophene-2-carboxamide for 2- (3- bromophenoxy) pyridine in EXAMPLE 21.
  • EXAMPLE 29 This example was prepared by substituting 5-bromo-N- (4- (1, 2, 3-thiadiazol-4-yl) benzyl) thiophene-2-carboxamide for 2- (3-bromophenoxy) pyridine in EXAMPLE 21.
  • EXAMPLE 30 This example was prepared by substituting 5-bromo-N- (3- (quinolin-3-yl) propyl) thiophene-2-carboxamide for 2- (3- bromophenoxy) pyridine in EXAMPLE 21.
  • EXAMPLE 31 This example was prepared by substituting N-benzyl-N- ( (5-bromothien-2-yl) methyl) -N-methylamine for 2-(3- bromophenoxy) pyridine in EXAMPLE 21.
  • EXAMPLE 32 A mixture of EXAMPLE 7, (300 mg) , N- (3-bromophenyl) -N ' - pyridin-4-ylurea (123 mg) , tris (dibenzylideneacetone) - dipalladium(O) (16.6 mg) , 1, 2-bis (diphenylphosphino) ethane (14.4 mg) , triethylamine (1.5 mL) , and copper (I) iodide (0.86 mg) in acetonitrile (5 mL) was heated at 75 °C for 18 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1:1 dichloromethane/ methanol/concentrated ammonium hydroxide.
  • EXAMPLE 33 A solution of EXAMPLE 6 (672 mg) in DMF (5 mL) at 0 °C was treated with 60% oily sodium hydride (70 mg) , stirred for 40 minutes, treated with N-fluorobenzenesulphonimide (314 mg) , stirred for 3 hours, diluted with ethyl acetate, washed with water and brine, dried (Na 2 S0 4 ) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 95:5:0.5 dichloromethane/methanol/ concentrated ammonium hydroxide.
  • EXAMPLE 18 13 3 C, NMR (CDCI 3 ) 5204.91, 204.85, 179.99, 179.83, 169.54, 169.37, 156.0, 155.97, 151.07, 150.89, 147.88, 143.78, 140.85, 136.39, 135.66, 135.42, 132.22, 131.65, 131.44, 130.21, 130.00, 129.53, 129.47, 129.32, 129.29, 128.02, 127.95, 126.77, 126.72, 103.61, 103.54, 94.03, 93.51, 81.51,
  • EXAMPLE 21 13 3 C, NMR (CDCI 3 ) ⁇ 204.8, 180.1, 169.6, 163.4, 155.9, 153.9, 147.7, 139.4, 129.5, 127.8, 124.4, 124.0, 121.3, 118.6, 111.5, 103.5, 88.1, 84.9, 81.4, 80.3, 77.5, 77.1, 70.2, 69.5, 65.9, 60.2, 51.3, 51.2, 49.3, 47.2, 42.6, 41.2, 40.2, 38.0, 36.2, 28.3, 22.2, 21.2, 20.2, 19.6, 15.2, 14.6, 13.0, 11.0, 10.4.
  • EXAMPLE 22 13 C NMR (CDCI 3 ) ⁇ 205.0, 180.6, 169.2, 164.5, 159.2,

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Abstract

Antibacterial compounds having formula (I), and salts, prodrugs, and salts of prodrugs thereof, processes for making the compounds and intermediates used in the processes, compositions containing the compounds, and methods for prophylaxis or treatment of bacterial infections using the compounds are disclosed.

Description

MACROLIDE ANTIBACTERIAL COMPOUNDS
TECHNICAL FIELD This invention is directed to compounds with antibacterial activity, processes for making the compounds and intermediates used in the processes, compositions containing the compounds, and methods for prophylaxis or treatment of bacterial infections using the compounds .
BACKGROUND OF THE INVENTION Because the effectiveness of many drugs currently available for prophylaxis or treatment of bacterial infections is being compromised by the emergence of drug-resistant bacteria, novel antibacterial compounds would be beneficial for their therapeutic value and their contribution to the antibacterial arts. SUMMARY OF THE INVENTION A first embodiment of this invention, therefore, is directed to compounds, and salts, prodrugs, and salts of prodrugs thereof, having antibacterial activity, the compounds having formula (I)
Figure imgf000003_0001
(I), in which two of A 1, B1, D1, and E1 are hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, -CN,
-OH, -SH, -C(0)H, -CCOJR1, -C(0)OH, -CfOJOR1, -C(0)NR2R3, or alkyl substituted by one, two, or three substituents independently selected from the group consisting of -CN, -OH, -SH, halo, aryl, heteroaryl, heterocyclyl, -OR , -SR , -C(0)H, -C(0)R1, -C(0)OH, -C(0)ORX, -CH=N-OR1, -OC (0) R1, -OC(0)OR1, -C(0)NR2R3, -OC(0)NR2R3, -NR2R3, -N(R4)C(0)H, -N(R4)C(0)R1, -N(R4)C(0)NR2R3, -N(R )S02R1, -OR1, -SR1, -S(0)R1, -SO2R1, and -S02NR2R3, and the remainder are hydrogen; or A1 and D1, A1 and E1, B1 and D1, or B1 and D1 together are one- to five-membered alkylene or two- to five-membered heteroalkylene, and the remainder are hydrogen; or
A and B together are one- to seven-membered alkylene or two- to seven-membered heteroalkylene, and D 1 and E1 are hydrogen; or D1 and E1 together are one- to seven-membered alkylene or two- to seven-membered heteroalkylene, and A and B are hydrogen;
X is hydrogen or fluoride; M1 is (E)-CH=CH, (Z)-CH=CH, or C≡C;
Y is arylene or heteroarylene;
L is drawn from left to right and is alkylene, alkenylene, alkynylene, CH=N-0-CH2- (alkenylene) , CH2N(R ), CH2N(R5) (CH2)m, C(0)N(R5), N (R5) C (0) N (R6) , CH=N-N (R5) , CH=N-N(R5)C(0) , 0, CH=N-0, CH=N-0- (CH2)m, C (0) N (R5) (CH2) m, or CH=N-0(CH2)n-0, in which m is one, two, three, or four, and n is two, three, or four;
W is hydrogen aryl, heteroaryl, or heterocyclyl; R is alkyl, aryl, heteroaryl, or heterocyclyl;
R 2 and R3 are independently hydrogen or alkyl; or
R 2 and R3 together are 3- to 7-membered alkylene or 3- to 7-membered heteroalkylene; R is hydrogen or alkyl; R and R are independently hydrogen or alkyl; and
R A is hydrogen or RP in whi.ch RP is a hydroxyl protecting moiety; in which, for the foregoing, each aryl, arylene, heteroaryl, heteroarylene, heterocyclyl, and heterocycloalkylene is unsubstituted or substituted by one, two, three, four, or five substituents independently selected from the group consisting of alkyl, alkenyl, alkynyl, cycloalkyl, halo, -CN, -OH, -SH, -NH2, -N02, =0, -CF3, -CH2CF3, -CF2CF3, -OCF3, -OCH2CF3, -OCF2CF3, -OR30, -SR30, -S(0)R35, -S02R35, -C(0)H, -C(0)R35, -C (0) OH, -C(0)OR35, -NH(R35), -N(R35) (R36) , -C(0)NH2, -C (0) NH (R35) , -C(0)N(R35) (R36) , -OC(0)R35, -OC(0)OR35, -OC(0)NH2, -0C(0)NH(R35) , -0C(0)N(R35) (R36) , -NHC(0)H, -NHC(0)R35, -NHC(0)0R35, -NHC(0)NH2, -NHC (0) NH (R35) , -NHC (0) N (R35) (R36) , -S02NH2, -S02NH(R35), -S02N (R35) (R36) , R40, and alkyl substituted with one or two substituents independently selected from the group consisting of halo, -CN, -OH, -SH, =0, -OR30, -SR30, -C(0)OH, -C(0)OR35, -NH2, -NH(R35), -N(R35) (R36) , -C(0)NH2, -C(0)NH(R35) , -C (0) N (R35) (R35) , -0C(0)R35, -OC(0)NH2, -OC(0)NH(R35) , -OC (0) N (R35) (R36) , -S02NH2, -S02NH(R35), -S02N (R35) (R36) , and R40;
R30 is alkyl or alkyl substituted with a substituent selected from the group consisti ■ng of halo and -OR45; R and R are independently selected alkyl; R is phenyl, naphthyl, furyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, 1, 2, 3-oxadiazolyl, 1, 2, 3-thiadiazolyl, 1, 3, -thiadiazolyl, 1, 2, 3-triazolyl, tetrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyrrolidinyl, inidazolidinyl, piperidinyl, piperazinyl, morpholinyl, or thiomorpholinyl, each of which is unsubstituted or substituted with one, two, or three substituents independently selected from the group consisting of alkyl, alkenyl, alkynyl, cycloalkyl, halo, -CN, -OH, -SH, -NC-2, =0, -CF3, ~CH2CF3, -CF2CF3, -0CF3, -OCH2CF3, -OCF2CF3, -OR45, -SR45, -S(0)R5°, -S02R5°, -C(0)H, -C(0)R50, -C(0)0H, -C(0)0R5°, -NH2, -NH(R50), -N (R50) (R51) , -C(0)NH2, -C(0)NH(R5°) , -C (0) N (R50) (R51) , -0C(0)R5°, -0C(0)0R5°, -0C(0)NH2, -0C(0)NH(R50) , -OC (0) N (R50) (R51) , -NHC(0)H, -NHC(0)R5°, -NHC (0) OR50, -NHC(0)NH2, -NHC (0) NH (R50) , -NHC(0)N(R50) (R51) , -S02NH2, S02NH(R5°), and -S02N (R50) (R51) ; R45 is alkyl; and R and R are independently selected alkyl. A second embodiment of this invention is directed to processes for making the compounds of the first embodiment. A third embodiment of this invention is directed to intermediates which are useful in the second embodiment. A fourth embodiment of this invention is directed to compositions comprising a therapeutically effective amount of a compound of the first embodiment.
A fifth embodiment of this invention is directed to methods for prophylaxis or treatment of bacterial infections in a fish or a mammal comprising administering thereto a therapeutically effective amount of a compound of the first ■embodiment .
In a preferred fifth embodiment of this invention, the beneficiary of prophylaxis or treatment of bacterial infections is a mammal.
In a more preferred fifth embodiment of this invention, the beneficiary of prophylaxis or treatment of bacterial infections is a human.
DETAILED DESCRIPTION OF THE INVENTION The compounds of this invention comprise both fixed and variable moieties, the latter of which are identified by a capital letter and accompanying numerical or alphabetical superscript, in which the term "alkenyl" means a monovalent, straight or branched hydrocarbon, having two to eight carbon atoms and at least one carbon-carbon double bond, attached through a carbon atom; the term "alkenylene" means a divalent, straight or branched hydrocarbon, having two to eight carbon atoms and one carbon-carbon double bond, attached through carbon atoms ; the term "alkynyl" means a monovalent, straight or branched hydrocarbon, having two to eight carbon atoms and at least one carbon-carbon triple bond, attached through a carbon atom; the term "alkynylene" means a divalent, straight or branched hydrocarbon, having two to eight carbon atoms and one carbon-carbon triple bond, attached through carbon atoms; the term "alkyl" means a monovalent, saturated, straight or branched hydrocarbon, having one to eight carbon atoms, attached through a carbon atom; the term "alkylene" means a divalent, saturated, straight or branched hydrocarbon, having one to eight carbon atoms, attached through carbon atoms; the term "aryl" means monovalent phenyl, attached through a carbon atom, unf sed or fused with cycloalkyl, cycloalkenyl, heteroaryl, another phenyl, naphthyl, or the saturated part of indan; the term "arylene" means divalent phenyl, attached through phenyl carbon atoms, unfused or fused with cycloalkyl, cycloalkenyl, another phenyl, naphthyl, or the saturated part of indan; the term "cycloalkenyl" means a monovalent, cyclic or bicyclic hydrocarbon, having four to eight carbon atoms and one or two carbon-carbon double bonds, attached through a carbon atom; the term "cycloalkyl" means a monovalent, saturated cyclic hydrocarbon, having three to eight carbon atoms, attached through a carbon atom; the term "cycloalkylene" means a divalent, saturated cyclic hydrocarbon, having three to eight carbon atoms, attached through carbon atoms; the term "halo" means fluoro (-F) , chloro (-C1) , bromo (-Br) , and iodo (-1) ; the term "heteroaryl" means a monovalent, aromatic, five-membered ring having two double bonds and one oxygen or one sulfur atom, one, two, three, or four nitrogen atoms, or one or two nitrogen atoms and one oxygen or one sulfur atom and the remaining atoms are carbon atoms, attached through a carbon or nitrogen atom, and unfused or fused with phenyl, cycloalkyl, cycloalkenyl, heterocycle, or another heteroaryl; and a monovalent aromatic, six-membered ring having three double bonds and one, two, or three nitrogen atoms and the remaining atoms are carbon atoms, attached through a carbon atom and unfused or fused with phenyl, cycloalkyl, cycloalkenyl, heterocycle, or another heteroaryl; the term "heteroarylene" means a divalent, aromatic, five-membered ring having two double bonds and one oxygen or one sulfur atom, one, two, three, or four nitrogen atoms, or one or two nitrogen atoms and one oxygen or one sulfur atom and the remaining atoms are carbon atoms, attached through carbon atoms; and a divalent aromatic, six-membered ring having three double bonds and one, two, or three nitrogen atoms and the remaining atoms are carbon atoms, attached through carbon atoms; the term "heterocyclyl" means a monovalent, non- aromatic three- or four-membered ring having one nitrogen, oxygen, or sulfur atom and the remaining atoms are carbon atoms, zero double bonds, attached through a carbon or nitrogen atom and unfused or fused with phenyl or heteroaryl; a monovalent, non-aromatic five-membered ring having one or two nitrogen, oxygen, or sulfur atoms, and the remaining atoms are carbon atoms, and zero or one double bonds, attached through a carbon or nitrogen atom and unfused or fused with phenyl or heteroaryl; and a monovalent, non-aromatic six or seven-membered ring having one, two, or three nitrogen, oxygen, or sulfur atoms and the remaining atoms are carbon atoms, and zero, one, or two double bonds, attached through a carbon or nitrogen atom and unfused or fused with phenyl or heteroaryl; and the term "heteroalkylene" means an alkylene of three to eight atoms, connected through a carbon atom, in which one, two, or three CH moieties have been independently replaced by 0, NH, N (alkyl), S, C(0), S (0) , or S02.
Preferred A I, Bl, Dl, and E1 moieties are hydrogen.
A preferred X moiety is hydrogen. A preferred M moiety is C≡C.
Preferred Y moieties are 1, 3-phenylene, 1, -phenylene, and 2, 5-thienylene .
Preferred L moieties are alkynylene, CH=N-0-CH2- (alkenylene) , CH2N(R5), CH2N (R5) (CH2) m, C(0)N(R5), N(R5)C(0)N(R6) , CH=N-N(R5), CH=N-N (R5) C (0) , 0, CH=N-0, CH=N-0(CH2)m, C(0)N(R5) (CH2)m, and CH=N-0 (CH2) n-0, in which m is one to three, and n is three.
Preferred moieties are phenyl, 3-fluorophenyl, 4- (1, 2, 3-thiadiazol-4-yl) phenyl, phenyl fused with another phenyl (naphthyl) , pyridyl, and pyridyl fused with phenyl (quinolinyl) .
Preferred R moieties are hydrogen and alkyl.
A preferred R moiety is hydrogen.
A preferred R A moi■ety i<s hydrogen. These preferred variable moieties combine with the parent moiety to form a preferred first embodiment of this invention, the preferred first embodiment comprising compounds, and salts, prodrugs, and salts of prodrugs thereof, having formula (I)
Figure imgf000010_0001
(I), in which A1, B1, D1, and E1 are hydrogen; X1 is hydrogen; M1 is C≡C; Y 1 is arylene or heteroarylene, in which the Y1 arylene is 1, 3-phenylene or 1, 4-phenylene, and in which the
Y 1 heteroarylene is 2, 5-thι.enylene; L1 i.s drawn from left to right and is alkynylene, CH=N-0-CH- (alkenylene) , CH2N(R5),
CH2N(R5) (CH2)m, C(0)N(R5), N (R5) C (0) N (R6) , CH=N-N(R5), CH=N-
N(R5)C(0), 0, CH=N-0, CH=N-0(CH2)m, C (0) N (R5) (CH2) m, or CH=N-0 (CH2) n _°f i-n which m is one, two, or three, and n is three; W is hydrogen, aryl, or heteroaryl, in which the aryl is phenyl or phenyl fused with another phenyl (naphthyl) , each of which is unsubstituted or substituted by one substituent selected from the group consisting of halo and R , in which R is 1, 2, 3-thiadiazolyl, and in which the heteroaryl is pyridyl or pyridyl fused with phenyl (quinolinyl) ; with the proviso that W is hydrogen only when L1 is CH=N-0 (CH2)m; R is hydrogen or alkyl; R is hydrogen; and R is hydrogen. These preferred variable moieties also combine to form another preferred first embodiment of this invention, the preferred first embodiment comprising compounds, and salts, prodrugs, and salts of prodrugs thereof, having formula (I)
Figure imgf000011_0001
(I), m whi■ch A1, B1, D1, and E1 are hydrogen; X1 is hydrogen; M1 is C≡C; Y is arylene or heteroarylene, m which the arylene is 1, 3-phenylene or 1, 4-phenylene, and in which the heteroarylene is 2, 5-thιenylene; L is drawn from left to right and is C2-alkynylene, CH=N-0-CH2- (C2-alkenylene) , CH2N(R5), CH2N(R5) (CH2)m, C(0)N(R5), N (R5) C (0) N (R6) , CH=N-N(R5), CH=N-N(R5)C(0) , 0, CH=N-0, CH=N-0 (CH2)m, C(0)N(R5) (CH2)m, or CH=N-0 (CH2) n-0, in which m is one, two, ι , or three, and n is three; W is hydrogen, aryl, heteroaryl, or heterocyclyl, in which the aryl is phenyl or phenyl fused with another phenyl (naphthyl) , each of which is unsubstituted or substituted by one substituent selected from the group consisting of halo and R , in which R is
1, 2, 3-thiadiazolyl, and in which the heteroaryl is pyridyl or pyridyl fused with phenyl (quinolinyl) ; with the proviso that W 1 is hydrogen only when L1 is CH=N-0(CH2)m and m is one; R is hydrogen or Ci-alkyl; R is hydrogen; and R is hydrogen.
These preferred variable moieties also combine to form still yet another preferred first embodiment of this invention, the preferred first embodiment comprising compounds, and salts, prodrugs, and salts of prodrugs thereof, which are (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6,8, 12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( (phenylamino) methyl) thien-2-yl) prop-2-ynyl) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( (E) - ( ( (phenylmethyl) oxy) imino)methyl) thien-2-yl) prop-2-ynyl) - 10, 13-dioxa-15, 18-diazatricyclo [10.6.2. o15,20] icos-1 (18) -en- 5-yl 3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl-
2, 4,6,8,12,19-hexamethyl-4- (3-(5-( (E) -
( (methyloxy) imino) methyl) thien-2-yl) prop-2-ynyl) -7,9,14- trioxo-10, 13-dioxa-15, 18-di ■azatricyclo [10.6.2.015' 20]ιcos-
1 (18) -en-5-yl 3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6,8,12,19-hexamethyl-7,9,14-trioxo-4-(3- (5- ( (E)- ( (phenyloxy) imino)methyl) thien-2-yl) prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl
3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4, 6, 8, 12, 19-hexamethyl-4- (3- (5- ( (E) - ( ( (naphthalen-1- ylmethyl) oxy) imino)methyl) thien-2-yl) prop-2-ynyl) -7, 9, 14- trioxo-10, 13-dioxa-15, 18-diazatricyclo [10.6.2.0 ' ] icos-
1 (18) -en-5-yl 3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6,8,12,19-hexamethyl-4-(3-(5-( (E)-( ( (3-naphthalen-l- ylprop-2-enyl) oxy) imino)methyl) thien-2-yl) prop-2-ynyl) - 7, 9,14-trioxo-10, 13-dioxa-15, 18- diazatricyclo [10.6.2.015'20] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12 S, 19R, 2 OR) -11-ethyl- 2,4,6, 8,12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( (E)- ( ( (2- (phenyloxy) ethyl) oxy) imino) methyl) thien-2-yl) prop-2-ynyl) - 10, 13-dioxa-15, 18-diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-
5-yl 3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2, 4, 6, 8,12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (5-
( ( (phenylmethyl) amino)methyl) thien-2-yl) prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-5-yl
3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6, 8,12,19-hexamethyl-7,9,14-trioxo-4-(3-(5- ( (E)-
(pyridin-2-ylhydrazono) methyl) thien-2-yl) prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-5-yl
3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, N ' - ( (IE) - (5- (3- ( (2R, R, 5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11- ethyl-2,4,6,8,12,19-hexame'thyl-7,9,14-trioxo-5- ( (3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) - 10, 13-dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en- 4-yl) prop-1-ynyl) thien-2-yl)methylidene) pyridine-2- carbohydrazide, (2R,4R,5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl-
2, 4, 6, 8,12,19-hexamethyl-7,9,14-trioxo-4-(3-(5- ( (E)-
( ( (quinolin-3-ylmethyl) oxy) imino)methyl) thien-2-yl) prop-2- ynyl) -10, 13-dioxa-15, 18-diazatricyclo [10.6.2.0 ' ] icos-
1 (18) -en-5-yl 3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6, 8,12,19-hexamethyl-7,9,14-trioxo-4-(3-(5- ( ( (2- phenylethyl) amino)methyl) thien-2-yl) prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18 ) -en-5-yl
3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R,20R) -11-ethyl- 2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( ( (3- phenylpropyl) amino)methyl) thien-2-yl) prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl
3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6, 8,12,19-hexamethyl-7,9,14-trioxo-4- (3- (3- (pyridin-2- yloxy) phenyl) prop-2-ynyl) -10, 13-dioxa-15, 18- diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
5- (3- ( (2R, R,5R, 6R, 8R, 11R, 12S, 19R, 2 OR) -11-ethyl- 2, 4, 6, 8, 12,19-hexamethyl-7, 9, 14-trioxo-5- ( (3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2.015'20] icos-1 (18 ) -en-4-yl) prop-1- ynyl) -N- (3-fluorophenyl) thiophene-2-carboxamide,
5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-5- ((3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2.015'20] icos-1 ( 18 ) -en-4-yl) prop-1- ynyl) -N- (3-fluorophenyl) -N-methylthiophene-2-carboxamide,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R,20R) -ll-ethyl-4- (3- (3- ( (3- fluorophenyl) oxy) phenyl) prop-2-ynyl) -2, 4, 6, 8, 12, 19- hexamethyl-7, 9, 14-trioxo-10, 13-dioxa-15, 18- diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2, 4, 6, 8, 12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- (pyridin-2- ylethynyl) thien-2-yl) prop-2-ynyl) -10, 13-dioxa-15, 18- \ diazatricyclo[10.6.2.015,20]icos-l(18)-en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6, 8,12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (4- (phenyloxy) phenyl) prop-2-ynyl) -10, 13-dioxa-15, 18- diazatricyclo [10.6.2.015'20] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2 OR) -11-ethyl- 2,4,6, 8,12,19-hexamethyl-7, 9, 14-trioxo-5- ( (3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa-
15, 18-diazatricyclo [10.6.2.015'20] icos-1 (18) -en-4-yl) prop- 1- ynyl) -N-pyridin-3-ylthiophene-2-carboxamide,
5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl- 2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-5- ( (3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa-
15, 18-diazatricyclo [10.6.2.015'20] icos-1 (18 ) -en-4-yl) prop-1- ynyl) -N- (4- (1,2, 3-thiadiazol-4-yl) phenyl) thiophene-2- carboxamide,
5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2 OR) -11-ethyl- 2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-5- ( (3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-4-yl) prop-1- ynyl) -N- (3-quinolin-3-ylpropyl) thiophene-2-carboxamide, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6,8,12,19-hexamethyl-4-(3-(5-
( (methyl (phenylmethyl) amino)methyl) thien-2-yl) prop-2-ynyl) - 7, 9, 14-trioxo-10,13-dioxa-15, 18- diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, and N- (5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl-
2,4,6,8,12,19-hexamethyl-7, 9, 14-trioxo-5- ( (3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18 ) -en-4-yl) prop-1- ynyl) thien-2-yl) -N ' -pyridin-4-ylurea .
The compounds of this invention comprise asymmetrically substituted carbon atoms in the R or S configuration. Asymmetric carbon atoms with equimolar amounts of R and S configurations are racemic. Atoms with an excess of one configuration over the other are assigned the configuration in the higher amount, preferably an excess of about 85%-90%, more preferably an excess of about 95%-99%, and still more preferably an excess greater than about 99%.
The terms "R" and "S" are as defined by the IUPAC 1974 Recommendations for Section E, Fundamental Stereochemistry, Pure Appl. Chem. (1976) 45, 13-10.
Accordingly, all stereoisomers of the compounds of this invention, including racemic mixtures, mixtures of diastereomers, and single diastereomers, are meant to be embraced by this invention.
The compounds of this invention may also comprise carbon-carbon double bonds as being in the Z or E configuration, in which the term "Z" represents the larger two of the four substituents disposed on same side of a carbon-carbon double bond and the term "E" represents the larger two of the four substituents disposed on opposit'e sides of a carbon-carbon double bond. The compounds may also exist as an equilibrium mixture comprising Z or E configurations .
The compounds of this invention containing hydroxyl, amino, or carboxylic acids may have attached thereto prodrug-forming moieties. The prodrug-forming moieties are removed by metabolic processes and release the compounds having the freed hydroxyl, amino, or carboxylic acid in vivo. Prodrugs are useful for adjusting such pharmacokinetic properties of the compounds as solubility and/or hydrophobicity, absorption in the gastrointestinal tract, bioavailabil'ity, tissue penetration, and rate of clearance .
The compounds of this invention may be prepared by synthetic processes or metabolic processes. Metabolic processes include those processes occurring in vitro in vivo .
The compounds of this invention may exist as acid addition salts, basic addition salts, or zwitterions . Salts of the compounds are prepared during their isolation or following their purification. Acid addition salts of the compounds are those derived from the reaction of the compounds with an acid. For example, the acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsufonate, digluconate, glycerophosphate, hemisulfate, heptanoate, hexanoate, formate, fumarate, hydrochloride, hydrobromide, hydroiodide, lactate, maleate, mesitylenesulfonate, methanesulfonate, naphthylenesulfonate, nicotinate, oxalate, pamoate, pectinate, persulfate, picrate, propionate, succinate, tartrate, thiocyanate, trichloroacetic, trifluoroacetic, phosphate, glutamate, bicarbonate, para- toluenesulfonate, lactobionate, and undecanoate salts of the compounds and prodrugs thereof are contemplated as being within the scope of this invention. When the compounds contain carboxylic acids, basic addition salts may be prepared therefrom by reaction with a base such as the hydroxide, carbonate, or bicarbonate of cations such as lithium, sodium, potassium, calcium, and magnesium.
The compounds of this invention may be administered with or without an excipient . Excipients include encapsulating materials or formulation additives such as absorption accelerators, antioxidants, binders, buffers, coating agents, coloring agents, diluents, disintegrating agents, emulsifiers, extenders, fillers, flavoring agents, humectants, lubricants, perfumes, preservatives, propellants, releasing agents, sterilizing agents, t sweeteners, solubilizers, wetting agents, and mixtures thereof. Excipients for orally administered compounds in solid dosage forms include agar, alginic acid, cocoa butter, gelatin, isotonic saline, malt, powdered tragacanth,
Ringer's solution, talc, water, aluminum hydroxide, magnesium hydroxide, sodium and potassium phosphate salts, cellulose, cellulose acetate, ethyl cellulose, sodium carboxymethyl cellulose, ethyl laureate, ethyl oleate, magnesium stearate, sodium lauryl sulfate, castor oil, corn oil, cottonseed oil, germ oil, groundnut oil, olive oil, peanut oil, safflower oil, sesame oil, soybean oil, benzyl alcohol, benzyl benzoate, 1,3-butylene glycol, ethanol, ethyl acetate, ethyl carbonate, glycerol, isopropanol, propylene glycol, tetrahydrofurfuryl alcohol, corn starch, potato starch, lactose, glucose sucrose, and mixtures thereof. Excipients for ophthalmically and orally administered compounds in liquid dosage forms include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, cottonseed oil, groundnut oil, corn oil, germ oil, olive oil, castor oil, sesame oil, glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols, fatty acid esters of sorbitan, and mixtures thereof. Excipients for osmotically administered compounds include water, ethanol, isopropanol, chlorofluorohydrocarbons, and mixtures thereof. Excipients for parenterally administered compounds include water, 1, 3-butanediol, Ringer's solution, U.S. P. or isotonic sodium chloride solution, oleic acid, castor oil, corn oil, cottonseed oil, germ oil, groundnut oil, olive oil, peanut oil, safflower oil, sesame oil, soybean oil, liposomes, and mixtures thereof. Excipients for rectally and vaginally administered compounds include cocoa butter, polyethylene glycol, wax, and mixtures thereof.
The compounds of this invention may be administered parenterally (subcutaneously, intravenously, intramuscularly, and intrasternally) , orally, osmotically, ophthalmically, rectally, topically, and vaginally. Orally administered compounds in solid dosage forms may be administered as capsules, dragees, granules, pills, powders, and tablets. Ophthalmically and orally administered compounds in liquid dosage forms may be administered as elixirs, emulsions, microemulsions, solutions, suspensions, and syrups. Osmotically and topically administered compounds may be administered as creams, gels, inhalants, lotions, ointments, pastes, powders, solutions, and sprays. Parenterally administered compounds may be administered as aqueous or oleaginous solutions or aqueous or oleaginous suspensions, the latter of which contains crystalline, amorphous, or otherwise insoluble forms of the compounds. Rectally and vaginally administered compounds may be administered as creams, gels, lotions, ointments, and pastes .
Dosage forms for the compounds of this invention depend on the species being treated, the disorder being treated and the severity thereof, the composition comprising the compounds, the time of administration, the route of administration, the duration of treatment, the potency of the compounds, and the rate of excretion of the compounds. The daily therapeutically effective amount of the compounds administered to a patient in single or divided doses range from about 0.1 to about 200 mg/kg body weight, preferably from about 0.25 to about 100 mg/kg body weight. Single dose compositions contain these amounts of the compounds or combinations of submultiples thereof. To determine antibacterial activity of the compounds of this invention, twelve petri dishes, each containing successive aqueous dilutions of test compounds in sterilized Brain Heart Infusion agar (Difco 0418-01-5) (10 L) , were inoculated with 1:100 dilutions of the representative microorganisms in TABLE 1 using a Steers replicator block (or 1:10 dilutions for slow-growing Streptococcus strains), co-incubated at 35-37 °C for 20-24 hours with a plate with an erythromycin A standard and a control plate with no compound, and inspected visually to provide the minimum inhibitory concentration (MIC) , in μg/mL, by which is meant the lowest concentration of the test compound which yielded no growth, a slight haze, or sparsely isolated colonies on the inoculum spot as compared to growth in the control plate.
TABLE 1
Microorganism Code
"Staphylococcus aureus NCTC10649M AA
Staphylococcus aureus A5177 BB
Staphylococcus' aureus PIU 2043 CC
Streptococcus pyrogenes EES61 DD
Streptococcus pyrogenes 930 EE
Streptococcus pyrogenes PIU 2548 FF
Streptococcus pneumoniae ATCC 6303 GG
Streptococcus pneumoniae 5979 HH
Streptococcus pneumoniae 5649 JJ
All of the compounds of this invention tested displayed antibacterial activity superior to their respective controls and are therefore useful as antibacterials .
The following schemes illustrate representative processes by which the compounds of this invention may be prepared, with the understanding that the order of the steps in the processes may be varied, other reagents may be substituted for those specifically mentioned, and vulnerable substituents may be protected and deprotected during the process . Abbreviations used are: DME for 1, 2-dimethoxyethane; DMF for N,N-dimethylformamide; and THF for tetrahydrofuran.
SCHEME 1
Figure imgf000022_0001
Compounds having formula (1) may be converted to p compounds having formula (2), in which R is acetyl (CH3C(0)), benzoyl (CeH5C(0)), or trimethylsilyl, by reacting the former, a hydroxyl protecting reagent, a first base, and, optionally, N,N-dimethylaminopyridine . Hydroxyl protecting reagents include benzoic anhydride, acetic anhydride, benzoyl chloride, acetyl chloride, and trimethylsilyl chloride. First bases include triethylamine, diisopropylethylamine, pyridine, and lutidine. The reaction is typically conducted at about 0 °C to 60 °C, over about 4 to 24 hours, in solvents such as dichloromethane, chloroform, THF, DME, and tert-butyl methylether.
Compounds having formula (2) may be converted to compounds having formula (3) by reacting the former, carbonyldiimidazole, a second base, and, optionally, N,N- dimethylaminopyridine . Second bases include 1,8- diazabicyclo- [5.4.0] undec-7-ene, lithium bis (trimethylsilyl) amide, sodium bis (trimethylsilyl) amide, and potassium bis (trimethylsilyl) amide . The reaction is typically conducted at about 25 °C, over about 6 to 24 hours, in solvents such as THF, DMF, 1,4-dioxane, and
N-methylpyrrolidine .
Compounds having formula (3) may be converted to compounds having formula (5) by (a) reacting the former and a compound having formula (4)
Figure imgf000023_0001
(4) and (b) reacting the product of step (a) with a dilute first acid. First acids include hydrochloric acid, triflic acid, para-toluenesulfonic acid, and trifluoroacetic acid.
Step (a) is typically conducted at about 25 °C, over about 24 hours to 72 hours, in solvents such as acetonitrile, DMF, water, and mixtures thereof. Step (b) is typically conducted at about 70 °C to 100 °C, over about 12 hours to about 24 hours, in solvents such as benzene, toluene, xylene, and mixtures thereof. Compounds having formula (5) may be converted to compounds having formula (6) by reacting the former and a second acid. Second acids include hydrochloric acid, triflic acid, para-toluenesulfonic acid, and trifluoroacetic acid. The reaction is typically conducted at about 60 °C, over about 12 to 24 hours, in solvents such as ethanol, acetone, THF, water, and mixtures thereof.
Compounds having formula (6) may be converted to compounds having formula (7) by reacting the former, a first oxidizing agent, and, optionally, a first additive. First oxidizing agents include dimethylsulfide/N- chlorosuccinimide, dimethylsulfoxide/1- (3- dimethylaminopropyl) -3-ethylcarbodiimide, and dimethylsulfoxide/oxalyl chloride. First additives include phosphoric acid, pyridinium trifluoroacetate, silica gel, triethylamine, and pyridine. The reaction is typically conducted at about -10 °C to 25 °C, over about 3 to 24 hours, in solvents such as THF, DMSO, and dichloromethane.
SCHEME 2
Figure imgf000024_0001
Compounds having formula (8) may be converted to compounds having formula (9) by reacting the former, a fluorinating agent, and, optionally, a third base. Fluorinating agents include 3, 5-dichloro-l-fluoropyridinium tetrafluoroborate, N-fluorobenzenesulfonimide, 3, 5-dichloro- l-fluoropyridinium triflate, N-fluoro-N-methyl-para- toluenesulfonamide, N-fluoropyridinium triflate, or N-fluoroperfluoropiperidine . Third bases include sodium hydride, potassium hydride, lithium diisopropylamide, triethylamine, and N,N-diisopropylethylamine. The reactions are typically conducted at about -78 °C to 0 °C, over about 2 to 24 hours, in solvents such as DMF, THF, and diethyl ether.
SCHEME 3
Figure imgf000025_0001
Compounds having formula (10) may be converted to compounds having formula (12) by reacting the former, a compound having formula (11)
Q1-Y1-C(0)H
(ID, in which Q1 is Cl, Br, or I, a first base, a coupling catalyst, and, optionally, a second additive. Coupling catalysts include dichlorobis (triphenylphosphine) palladium (II) , tris (dibenzylideneacetone) dipalladium(O) , tetrakis (triphenylphosphine) palladium (0) , and dichlorobis (triphenylphosphine) nickel (II) . Second additives include triphenylphosphine, triphenylarsine, 1,2- bis (diphenylphosphino) butane, 1, 2- bis (diphenylphosphino) ethane, copper (I) iodide, and mixtures thereof. The reactions are typically conducted at about 50 °C to 80 °C, over about 12 to 48 hours, in solvents such as acetonitrile, DME, THF, and mixtures thereof.
Compounds having formula (12) may be converted to compounds having formula (13) by reacting the former and a second oxidizing agent. Second oxidizing agents include molecular oxygen, potassium permanganate, sodium chlorite, and silver oxide. The reactions are typically conducted at about 0 °C to 35 °C, over about 1 to 48 hours, in solvents such as acetonitrile, DME, THF, tert-butanol, water, and mixtures thereof.
SCHEME 4
Figure imgf000027_0001
(D-b Compounds having formula (12) may be converted to compounds having formula (I) -a by reacting the former and a compound having formula (14)
HN(R5)-Q2-(CH2)Z-Q3-W1 (14) in which Q 2 is absent, 0, or N(R5); z is zero to four;
3 Q is absent, 0, or alkenylene; and in which Q , z, and Q may combine with the fixed moieties between Y 1 and W1 to form moieties represented by L , and the dilute first acid. The reaction is typically conducted at about 25 °C, over about 24 hours to 72 hours, in solvents such as acetonitrile, DMF, water, and mixtures thereof.
Compounds having formula (I) -a may be converted to compounds having formula (I)-b by reacting the former and a reducing agent. Reducing agents include sodium cyanoborohydride and sodium borohydride. The reaction is typically conducted at about 0 °C to 25 °C, over about 24 hours to 72 hours, in solvents such as methanol, ethanol, iso-propyl alcohol, acetonitrile, DMF, water, and mixtures thereof .
SCHEME 5
Figure imgf000028_0001
Compounds having formula (13) may be converted to compounds having formula (I)-c by reacting the former, a compound having formula (15)
HN(R5) (CH2)Z-Q3-WX (15), and a dehydrating agent. Dehydrating agents include 1,3- dicyclohexylcarbodiimide and 1- (3-dimethylaminopropyl) -3- ethylcarbodiimide . The reaction is typically conducted at about 0 °C to 25 °C, over about 24 hours to 72 hours, in solvents such as acetonitrile, DMF, water, and dichloromethane .
Compounds having formula (13) may be converted to compounds having formula (I)-d by (a) reacting the former and diphenylphosphoryl azide to form a compound having formula (16) and (b) reacting the product of step (a) with a compound having formula (17)
HN(R6) (W1) (17) . Step (a) is typically conducted at about 25 °C to 80 °C, over about 1 to 8 hours, in solvents such as benzene, toluene, and acetonitrile. Step (b) is typically conducted at about 25 °C to 100 °C, over about 1 to 24 hours, in solvents such as benzene, toluene, and acetonitrile, DMF, and DME .
SCHEME 6
Figure imgf000030_0001
Compounds having formula (10) may be converted to compounds having formula (19) by reacting the former and a compound having formula (18)
Br-Y1-Br (18) under the same conditions described for the conversion of compounds of this invention having formula (10) to compounds having formula (12) in Scheme 3.
SCHEME 7
Figure imgf000031_0001
(D-g Compounds having formula (19) may be converted to compounds having formula (I)-f by reacting the former and a compound having formula (20)
H-C≡C- (CH^y-W1 (20), in which y is zero to four, or compounds having formula (I)-g by reacting the former and a compound having formula (21)
H-CH=CH-(CH2)y-W1 (21), under the same conditions described for the conversion of compounds having formula (10) to compounds having formula (12) in Scheme 3. SCHEME 8
Figure imgf000032_0001
Compounds having formula (10) may be converted to compounds having formula (I)-h by reacting the former and a compound having formula (22)
Q1~Y1-L1-W1 (22) under the same conditions described for the conversion of compounds having formula (10) to compounds having formula (12) in Scheme 3.
SCHEME 9
Figure imgf000032_0002
(I)-h (I)-i
Compounds having formula (I)-h may be converted to compounds having formula (I)-i by reacting the former, hydrogen gas, a hydrogenation catalyst, and, optionally, quinoline. Hydrogenation catalysts include Lindlar catalyst and palladium on barium sulfate. The reaction is typically conducted at 25 °C, over about 1 to 6 hours, in solvents such as methanol, ethanol, propanol, butanol, iso-propanol, tert-butanol, acetonitrile, THF, ethyl acetate, and mixtures thereof.
SCHEME 10
Figure imgf000033_0001
(D-j
Compounds having formula (10) may be converted to compounds having formula (24) by reacting the former and compounds having formula (23) < B(V1)3,
(23) in which each V1 is independently hydrogen, alkyl, OH, or OR45. The reaction is typically conducted at about -20 °C to 25 °C, over about 1 to 6 hours, in solvents such as THF, DME, and diethyl ether. Compounds having formula (24) may be converted to compounds having formula (I)-j by reacting the former, compounds having formula (25)
X1-Y1-L1-W1 (25), a coupling catalyst, a fourth base, and, optionally, a second additive. Coupling catalysts include dichlorobis (triphenylphosphine) palladium (II) , tris (dibenzylideneacetone) dipalladium(O) , tetrakis (triphenylphosphine) palladium(0) , and dichlorobis (triphenylphosphine) nickel (II) . Fourth bases include sodium carbonate, sodium bicarbonate, potassium carbonate, cesium carbonate, triethylamine, and diisopropylethylamine . The reaction is typically conducted at about 50 °C to 80 °C over about 12 to 48 hours, in solvents such as acetonitrile, THF, DMF, and DME.
Compounds having formula (I) , m which R A is RP, and RP is acetyl or benzoyl, may be converted to compounds having formula (I) , in which R is hydrogen, by reacting the former and methanol. The reaction is typically conducted at about 25 °C to 65 °C, over about 2 to 60 hours, in methanol.
Compounds having formula (I) , m whi .ch RA is RP, and RP is trimethylsilyl, may be converted to compounds having formula (I) , in which RA is hydrogen, by reacting the former and a fluoride-donating agent. Fluoride-donating agents include tetrabutylammonium fluoride, polymer-bound ammonium fluoride, tetrabutylammonium fluoride, pyridine -HF, and triethylamine -trihydrofluoride . The reaction is typically conducted at about 0 °C to 50 °C, over about 1 to 24 hours, in solvents such as THF and 1,4-dioxane. The following examples illustrate methods by which certain preferred first embodiments of this invention may be prepared.
EXAMPLE 1 This example was prepared as described in commonly owned US 6,075,133, EXAMPLE 246, step 246c.
EXAMPLE 2 A solution of EXAMPLE 1 (10 g) , 4-dimethyl- aminopyridine (50 mg) , benzoic anhydride (7.02 g) , and triethylamine (3.8 mL) in dichloromethane (70 mL) at 15 °C was stirred for 20 minutes at 15 °C and at ambient temperature for 7 hours, diluted with ethyl acetate, washed with 5% sodium carbonate, water, and brine, and dried (Na S04) , filtered, and concentrated.
EXAMPLE 3' A solution of EXAMPLE 2 (9.80 g) , carbonyldiimidazole (4.05 g) , and 4-dimethylaminopyridine (122 mg) in THF (45 mL) and DMF (13 mL) was treated with 1, 8-diazabicyclo-
[5.4.0] undec-7-ene (2.24 mL) , stirred at ambient temperature for 12 hours, diluted with ethyl acetate, washed with water and brine, and dried (Na S04), filtered, and concentrated.
EXAMPLE 4
A solution of EXAMPLE 3 (11.09 g) and ethylenediamine (6.67 mL) in acetonitrile (50 mL) and water (5 mL) was stirred at ambient temperature for 3 days and concentrated. The concentrate was dissolved in toluene (140 mL) and acetic acid (7 mL) , stirred at 80 °C for 12 hours and at ambient temperature for 12 hours, diluted with dichloromethane, washed with saturated potassium carbonate, and dried (Na S04) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 95:5:0.5 dichloromethane/methanol/concentrated ammonium hydroxide.
EXAMPLE 5 A solution of EXAMPLE 4 (5.95 g) and 2M HCl (5 mL) in ethanol (5 mL) was heated to 55 °C for 12 hours and concentrated. The concentrate was dissolved in water, washed with diethyl ether, treated with saturated ammonium hydroxide and extracted with dichloromethane; and the extract was concentrated.
EXAMPLE 6 A solution of N-chlorosuccinimide (5.46 g) in dichloromethane (200 mL) at -10 °C was treated with dimethyl sulfide (3.50 mL) , stirred for 10 minutes, treated with a solution of EXAMPLE 5 (20.9 g) in dichloromethane (90 mL) over 30 minutes, stirred for 1 hour, treated with triethylamine (3.79 mL) , stirred for 90 minutes, washed with 5% sodium bicarbonate and brine, and dried (NaS04) , filtered, and concentrated.
EXAMPLE 7 A solution of EXAMPLE 6 (14 g) in methanol (100 mL) was heated at 60 °C for 16 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 97:2:1 dichloro ethane/methanol/concentrated ammonium hydroxide .
EXAMPLE 8 A mixture of EXAMPLE 7 (3.75 g) , 5-bromothiophene-2- carboxaldehyde (1.55 g) , dichlorobis (triphenylphosphine) - palladium (II) (76 mg) , and copper(I) iodide (10.2 mg) in acetonitrile (60 mL) and triethylamine (20 mL) was heated at 75 °C for 14 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 97:2:1 dichloromethane/methanol/concentrated ammonium hydroxide.
EXAMPLE 9 A mixture of EXAMPLE 8 (350 mg) , aniline (82.9 μL) , and acetic acid (77.8 μL) in methanol (8 mL) was stirred at ambient temperature for 2 hours, treated with sodium cyanoborohydride (57 mg) , heated at 60 °C for 1 hour, treated with dichloromethane, washed with 5% sodium bicarbonate and brine, and dried (Na2S04) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1:1 dichloromethane/methanol/ concentrated ammonium hydroxide.
EXAMPLE 10
A mixture of EXAMPLE 8 (280 mg) , and O-benzylhydroxyl- amine hydrochloride (86.6 mg) in methanol (4 mL) was heated at 55 °C for 2 hours, poured into 5% sodium bicarbonate, and extracted with dichloromethane. The extract was washed with brine, dried (NaS04) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 97.5:2:0.5 dichloromethane/methanol/concentrated ammonium hydroxide .
EXAMPLE 11
This example was prepared by substituting 0- phenylhydroxylamine hydrochloride for O-benzylhydroxylamine hydrochloride in EXAMPLE 8.
EXAMPLE 12
This example was prepared by substituting O-naphthalen- 1-ylmethylhydroxylamine and catalytic para-toluenesulfonic acid for O-benzylhydroxylamine hydrochloride in EXAMPLE 8. EXAMPLE 13 This example was prepared by substituting 0-(3- naphthalen-1-yl-allyl) hydroxylamine for O-naphthalen-1- ylmethylhydroxylamine in EXAMPLE 12.
EXAMPLE 14 This example was prepared by substituting O- (2-phenoxy- ethyl) hydroxylamine for O-naphthalen-1-ylmethylhydroxylamine hydrochloride in EXAMPLE 12.
EXAMPLE 15 A mixture of EXAMPLE 7 (350 mg) , N-benzyl-N- ( (5- bromothien-2-yl) ethyl) amine (172 mg) , dichlorobis- (triphenylphosphine) palladium (II) (7.4 mg) , triethylamine (2 mL) and copper (I) iodide (2 mg) in acetonitrile (6 mL) was heated at 80 °C for 20 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1.5:0.5 dichloromethane/methanol/concentrated ammonium hydroxide.
EXAMPLE 16 A mixture of EXAMPLE 8 (200 mg) , 2-hydrazinopyridine (42.3 mg) , and magnesium sulfate (50 mg) in THF was heated at 66 °C for 16 hours, and filtered. The filtrate was treated with ethyl acetate, washed with water and brine, dried (Na S04) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 97.5:2:0.5 dichloromethane/methanol/concentrated ammonium hydroxide.
EXAMPLE 17 This example was prepared by substituting pyridine-2- carbohydrazide for 2-hydrazinopyridine in EXAMPLE 16. EXAMPLE 18 This example was prepared by substituting O-quinol-3- ylmethylhydroxylamine for O-naphthalen-1- ylmethylhydroxylamine hydrochloride in EXAMPLE 12.
EXAMPLE 19 This example was prepared by substituting N-((5- bromothien-2-yl) methyl) -N- (2-phenylethyl) amine for N-benzyl- N- ( (5-bromothien-2-yl) methyl) amine in EXAMPLE 15.
EXAMPLE 20 This example was prepared by substituting N-((5- bromothien-2-yl) methyl) -N- (3-phenylpropyl) amine for N- benzyl-N- ( (5-bromothien-2-yl) methyl) amine in EXAMPLE 15.
EXAMPLE 21 A mixture of EXAMPLE 7 (350 mg) , 2- (3- bro ophenoxy) pyridine (251 mg) , dichlorobis (triphenyl- phosphine) palladiu (II) (11 mg) , and copper (I) iodide (1 mg) , and triethylamine (2 mL) in acetonitrile (6 mL) was heated at 70 °C for 20 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1.5:0.5 dichloromethane/methanol/concentrated ammonium hydroxide.
EXAMPLE 22 This example was prepared by substituting 5-bromo-N- (3- fluorophenyl) thiophene-2-carboxamide for 2- (3- bromophenoxy) pyridine in EXAMPLE 21.
EXAMPLE 23 This example was prepared by substituting 5-bromo-N- (3- fluorophenyl) -N-methylthiophene-2-carboxamide for 2- (3- bromophenoxy) pyridine in EXAMPLE 21.
EXAMPLE 24
This example was prepared by substituting l-bromo-3- (3- fluorophenoxy) benzene for 2- (3-bromophenoxy) pyridine in EXAMPLE 21.
EXAMPLE 25
A solution of EXAMPLE 6 (5 g), 2 , 5-dibromothiophene (4.74 g) , dichlorobis (triphenylphosphine) palladium (II) (184 mg) , and copper (I) iodide (17 mg) , and triethylamine (9 mL) in acetonitrile (27 mL) was heated at 80 °C for 24 hours, concentrated, treated with dichloromethane, washed with 5% i sodium bicarbonate and brine, dried (Na S04) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 98.5:1:0.5 dichloromethane/methanol/ concentrated ammonium hydroxide .
EXAMPLE 26 A solution of EXAMPLE 25, (300 mg) , 2-ethynylpyridine (41.7 mg) , dichlorobis (triphenylphosphine) palladium(II) (4.5 mg) , copper (I) iodide (0.6 mg) and triethylamine (1 mL) in acetonitrile (3 mL) was heated at 75 °C for 14 hours and concentrated .
EXAMPLE 26A A solution of EXAMPLE 26 in methanol (10 mL) was heated at 60 °C for 14 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1:1 dichloromethane/methanol/concentrated ammonium hydroxide.
EXAMPLE 27 I
This example was prepared by substituting l-bromo-4- phenoxybenzene for 2- (3-bromophenoxy) pyridine in EXAMPLE 21,
EXAMPLE 28 This example was prepared by substituting 5-bromo-N- pyridin-3-ylthiophene-2-carboxamide for 2- (3- bromophenoxy) pyridine in EXAMPLE 21.
EXAMPLE 29 This example was prepared by substituting 5-bromo-N- (4- (1, 2, 3-thiadiazol-4-yl) benzyl) thiophene-2-carboxamide for 2- (3-bromophenoxy) pyridine in EXAMPLE 21.
EXAMPLE 30 This example was prepared by substituting 5-bromo-N- (3- (quinolin-3-yl) propyl) thiophene-2-carboxamide for 2- (3- bromophenoxy) pyridine in EXAMPLE 21.
EXAMPLE 31 This example was prepared by substituting N-benzyl-N- ( (5-bromothien-2-yl) methyl) -N-methylamine for 2-(3- bromophenoxy) pyridine in EXAMPLE 21.
EXAMPLE 32 A mixture of EXAMPLE 7, (300 mg) , N- (3-bromophenyl) -N ' - pyridin-4-ylurea (123 mg) , tris (dibenzylideneacetone) - dipalladium(O) (16.6 mg) , 1, 2-bis (diphenylphosphino) ethane (14.4 mg) , triethylamine (1.5 mL) , and copper (I) iodide (0.86 mg) in acetonitrile (5 mL) was heated at 75 °C for 18 hours and concentrated; and the concentrate was flash chromatographed on silica gel with 98:1:1 dichloromethane/ methanol/concentrated ammonium hydroxide.
EXAMPLE 33 A solution of EXAMPLE 6 (672 mg) in DMF (5 mL) at 0 °C was treated with 60% oily sodium hydride (70 mg) , stirred for 40 minutes, treated with N-fluorobenzenesulphonimide (314 mg) , stirred for 3 hours, diluted with ethyl acetate, washed with water and brine, dried (Na2S04) , filtered, and concentrated; and the concentrate was flash chromatographed on silica gel with 95:5:0.5 dichloromethane/methanol/ concentrated ammonium hydroxide.
SPECTRAL DATA FOR REPRESENTATIVE COMPOUNDS
EXAMPLE 7
13C NMR (CDC13) δ 204.7, 169.5, 156.0, 103.4, 81.59, 81.53, 80.36, 77.2 (C-5) , 77.2 (C-13) , 74.3, 70.26, 69.6, 65.9, 60.1, 51.1, 50.6, 49.3, 47.1, 42.6, 42.2, 40.2, 40.2, 37.9, 36.3, 28.3, 22.3, 21.2, 20.3, 19.6, 15.0, 14.5, 12.9, 11.0, 10.6.
EXAMPLE 10 13C NMR (CDCI3) δ 204.88, 204.85, 179.86, 179.80; 169.56,169.53, 156.0, 143.16, 140.37, 132.18, 131.31,
131.28, 128.95, 128.45, 128.42, 128.02, 127.93, 103.60, 103.56, 93.97, 93.34, 81.48, 80.43, 78.94, 77.45, 77.34, 77.29, 76.87, 76.71, 70.32, 69.64, 65.89, 60.25, 51.41, 51.20, 49.47, 47.40, 47.32, 42.71, 42.28, 40.24, 37.99, 36.29, 28.20, 22.29, 21.25, 20.26, 19.65, 15.39, 15.28, 14.57, 13.03, 11.09, 10.53.
EXAMPLE 11 13C NMR (CDCI3) δ 204.86, 180.02, 179.94, 169.62, 169.59, 156.00, 145.61, 142.32, 132.54, 132.29, 131.39, 131.36, 130.40, 129.33, 129.29, 122.86, 122.41, 114.91, 114.42, 103.60, 103.54, 94.59, 93.88, 81.51, 80.49, 78.74, 77.43, 77.40, 77.28, 70.30, 69.66, 65.87, 51.45, 51.39, 51.20, 49.50, 49.47, 47.40, 47.29, 42.73, 42.28, 40.24, 38.02, 37.99, 36.31, 28.20, 22.27, 21.25, 20.26, 20.22, 19.65, 15.36, 15.23, 14.60, 13.03, 11.08, 10.53.
EXAMPLE 12
13C NMR (CDC13) δ 204.87, 204.83, 179.76, 179.73, 169.52, 156.0, 143.19, 140.19, 136.79, 133.72, 132.18, 131.86, 131.31, 131.28, 129.11, 129.01, 128.53, 128.49, 127.62, 127.12, 126.35, 125.78, 125.26, 124.11, 123.99, 103.60, 103.54, 93.92, 93.37, 81.48, 81.43, 80.45, 80.41, 78.94, 78.89, 77.45, 77.32, 77.307, 75.41, 75.08, 70.30, 69.64, 65.87, 60.23, 51.39, 51.20, 51.17, 49.47, 49.39, 47.40, 47.29, 42.69, 42.28, 42.23, 40.22, 37.96, 36.29, 28.19, 22.27, 21.24, 20.26, 19.64, 15.39, 15.23, 14.55, 13.023, 11.06, 10.51.
EXAMPLE 13 13C NMR (CDCI3) δ 204.89, 204.85, 179.79, 169.45, 156.0, 155.97, 143.19, 143.15, 140.29, 132.19, 131.33, 131.28, 130.90, 130.45, 128.98, 128.43, 128.16, 128.06, 128.12, 128.09, 126.05, 125.73, 125.69, 125.57, 124.08, 123.89, 123.85, 103.60, 103.54, 93.88, 93.37, 81.48, 80.46, 80.39, 79.0, 77.45, 77.34, 77.27, 77.29, 75.71, 75.30, 70.30, 69.64, 65.89, 60.25, 51.41, 51.22, 51.17, 49.49, 49.41, 47.42, 47.32, 42.69, 42.28, 40.22, 38.00, 36.29, 28.20, 22.32, 22.27, 21.24, 20.28, 19.63, 15.39, 15.25, 14.57, 13.06, 13.04, 11.08, 10.56.
EXAMPLE 14 13C NMR (CDCI3) δ 204.89, 204.85, 179.86, 179.80,169.56, 169.45, 156.0, 155.97, 143.57, 140.48, 132.21, 131.87, 131.37, 131.33, 129.40, 129.16, 120.89, 114.81, 114.70, 103.62, 103.57, 93.92, 93.42, 81.48, 80.46, 80.39, 78.89, 77.43, 77.36, 77.31, 77.28, 73.12, 72.84, 70.30, 69.66,
66.23, 65.91, 60.25, 51.41, 51.22, 51.19, 49.49, 49.43,
47.44, 47.37, 42.69, 42.27, 40.24, 37.99, 36.29, 28.20, 22.32, 22.27, 21.25, 20.226, 19.63, 15.43, 15.29, 14.56, 13.06, 13.04, 11.08, 10.56.
EXAMPLE 15 13C NMR (CDC13) δ 204.9, 179.8, 169.6, 156.0, 146.4, 139.8, 132.1, 128.4, 128.4, 128.1, 128.1, 126.9, 124.6, 121.5, 103.5, 90.9, 81.5, 80.2, 79.4, 77.4, 77.3 70.3, 69.6, 65.9, 60.2, 52.6, 51.4, 51.2, 49.4, 47.6, 47.4, 42.7, 42.2, 40.2, 38.0, 36.2, 28.2, 22.3.
EXAMPLE 16 13C NMR (CDCI3) δ 204.88, 204.85, 180.04, 179.83, 169.51, 169.48, 156.0, 147.61, 147.63, 138.15, 138.09, 137.97, 133.00, 132.54, 130.40, 129.30, 126.78, 116.67, 115.23, 107.94, 107.96, 103.57, 94.34, 93.03, 81.53, 81.49, 80.48, 80.38, 79.28, 78.28, 77.40, 77.31, 70.30, 69.64, 65.88, 60.23, 51.39, 51.20, 49.47, 47.40, 42.68, 42.26, 40.22, 37.99, 36.28, 28.20, 25.23, 22.29, 21.25, 20.28, 20.25, 19.62, 15.39, 15.35, 14.55, 13.04, 11.08, 10.54.
EXAMPLE 17 13C NMR (CDCI3) δ 204.91, 180.07, 169.39, 156.10,
132.67, 103.59, 81.66, 80.43, 77.36, 77.28, 70.32, 69.66, 65.87, 60.26, 51.39, 51.19, 49.39, 47.42, 42.65, 42.24,
40.24, 38.00, 36.23, 28.20, 22.35, 21.25, 20.26, 19.60, 15.38, 14.54, 13.08, 11.09, 10.59.
EXAMPLE 18 13 3C, NMR (CDCI3) 5204.91, 204.85, 179.99, 179.83, 169.54, 169.37, 156.0, 155.97, 151.07, 150.89, 147.88, 143.78, 140.85, 136.39, 135.66, 135.42, 132.22, 131.65, 131.44, 130.21, 130.00, 129.53, 129.47, 129.32, 129.29, 128.02, 127.95, 126.77, 126.72, 103.61, 103.54, 94.03, 93.51, 81.51,
80.46, 80.39, 78.86, 77.39, 77.28, 77.19, 77.21, 74.46, 74.15, 70.30, 69.64, 65.89, 60.23, 51.39, 51.20, 51.16,
49.47, 49.42, 47.42, 47.34, 42.69, 42.26, 42.23, 40.24, 38.00, 37.97, 36.28, 28.20, 22.36, 22.29, 21.24, 20.28, 20.23, 19.63, 15.41, 15.25, 14.54, 13.10, 13.04, 11.09, 10.58.
EXAMPLE 19 13 3C, NMR (CDC13) δ 204.9, 179.8, 169.6, 156.0, 146.4, 139.8, 132.1, 128.4, 128.4, 128.1, 128.1, 126.9, 124.6, 121.5, 103.5, 90.9, 81.5, 80.2, 79.4, 77.4, 77.3 70.3, 69.6, 65.9, 60.2, 52.6, 51.4, 51.2, 49.4, 47.6, 47.4, 42.7, 42.2, 40.2, 38.0, 36.2, 28.2, 22.3.
EXAMPLE 20 13C NMR (CDCI3) δ 204.9, 179.8, 169.3, 156.0, 146.6, 142.1, 132.1, 128.4 128.3, 125.7, 124.5, 121.5, 103.6, 91.0,
81.5, 80.2, 79.4, 77.4, 77.3, 70.3, 69.7, 69.6, 65.9, 60.2,
51.4, 51.2, 49.4, 48.5, 48.5, 47.4, 42.7, 42.2, 40.2, 38.0,
36.2, 33.5, 31.6, 28.2, 22.3, 21.2, 20.3, 19.6, 15.4, 14.5, 13.1, 11.1.
EXAMPLE 21 13 3C, NMR (CDCI3) δ 204.8, 180.1, 169.6, 163.4, 155.9, 153.9, 147.7, 139.4, 129.5, 127.8, 124.4, 124.0, 121.3, 118.6, 111.5, 103.5, 88.1, 84.9, 81.4, 80.3, 77.5, 77.1, 70.2, 69.5, 65.9, 60.2, 51.3, 51.2, 49.3, 47.2, 42.6, 41.2, 40.2, 38.0, 36.2, 28.3, 22.2, 21.2, 20.2, 19.6, 15.2, 14.6, 13.0, 11.0, 10.4. EXAMPLE 22 13C NMR (CDCI3) δ 205.0, 180.6, 169.2, 164.5, 159.2,
156.3, 139.4, 139.3, 138.8, 132.7, 130.1, 129.9, 129.4,
127.6, 115.5, 115.5, 111.3, 111.0, 107.8, 107.5, 103.6, 93.7, 81.8, 80.4, 78.5, 77.7, 77.1, 72.2, 70.2, 69.6, 65.9,
60.3, 51.3, 51.2, 49.3, 47.6, 42.6, 42.4, 40.2 38.1, 36.1,
28.2, 22.5, 21.2, 20.3, 19.6, 15.6, 13.2, 11.2, 10.7.
EXAMPLE 23 13C NMR (CDCI3) δ 204.8, 179.7, 169.6, 164.7, 161.8,
155.9, 145.3, 145.2, 137.7, 132.3, 131.5, 131.1, 127.9, 123.9, 115.7,115.5, 115.4, 115.3, 103.6, 93.7, 81.4, 80.4,
78.3, 77.4, 77.2, 70.3, 69.6, 65.9, 60.1, 51.3, 51.2, 49.4, 47.3, 42.6, 42.2, 40.2, 38.9, 37.9, 36.2, 28.1, 22.2, 21.2, 20.2, 19.6, 15.3, 14.5, 12.9, 11.0, 10.5.
EXAMPLE 24 13C NMR (CDCI3) δ 204.9, 179.8, 169.6, 160.5, 157.5, 156.0, 129.6, 126.3, 124.5, 121.0, 120.8, 120.7, 118.8, 118.4, 116.5, 116.2, 103.5, 88.0, 84.9, 81.5, 80.3, 77.4,
77.2, 70.3, 69.6, 65.9, 60.2, 51.3, 51.2, 49.4, 47.2, 42.7,
42.2, 40.2, 38.0, 36.3, 28.2, 22.3, 21.2, 20.2, 19.6, 15.2, 14.6, 13.0, 11.1, 10.5.
EXAMPLE 25
13C NMR (100 MHz, CDC13) δ 204.9, 179.9, 169.6, 156.0, 150-.1, 142.9, 136.1, 133.0, 132.1, 127.1, 125.3, 123.4, 122.9, 103.6, 92.9, 92.8, 82.2, 81.5, 80.5, 78.6, 77.4,
77.3, 70.3, 69.7, 65.9, 60.2, 51.4, 51.2, 49.5, 47.3, 42.7, 42.3, 40.2, 38.0, 36.3, 28.2, 22.3, 21.2, 20.2, 19.7, 15.3,
14.6, 13.0, 11.0, 10.5.
EXAMPLE 27 13C NMR (CDCI3) δ 204.9, 179.9, 169.5, 157.4, 156.0,
133.1, 129.8, 123.7, 119.3, 118.8, 118.3, 117.6, 103.5, 86.7, 85.1, 81.5, 80.2, 77.4, 77.3, 70.3, 69.6, 65.9, 60.2, 51.4, 51.2, 49.4, 47.3, 42.7, 42.2, 40.2, 38.1, 36.3, 28.2, 22.3, 21.2, 20.2, 19.6, 15.3, 14.6, 13.1, 11.1, 10.6.
EXAMPLE 28 13C NMR (CDCI3) δ 204.9, 180.3, 169.1, 159.7, 156.3,
145.2, 141.7, 138.8, 135.0, 132.6, 129.4, 127.9, 127.7, 123.6, 103.6, 93.8, 81.8, 80.4, 78.4, 77.6, 77.1, 70.3,
69.6, 65.8, 60.3, 57.3, 51.2, 49.3, 47.5, 42.6, 42.3, 40.2,
38.0, 36.1, 28.2, 22.4, 21.2, 20.3, 19.5, 15.5, 14.5, 13.1, 11.2, 10.6.
EXAMPLE 29
13C NMR (CDCI3) δ 204.8, 180.0, 169.4, 162.4, 161.1, 156.1, 139.4, 138.8, 132.5, 130.0, 128.5, 128.5, 127.7, 127.1, 103.6, 93.5, 81.6, 80.4, 78.4, 77.4, 77.3, 70.3, 69.6, 65.8, 60.2, 51.3, 51.2, 49.4, 47.4, 43.6, 42.6, 42.2, 40.2, 38.0, 36.2, 28.2, 22.3, 21.2, 20.2, 19.6, 15.4, 14.5,
11.1, 10.6.
EXAMPLE 30 13C NMR (CDCI3) δ 204.9, 180.2, 169.3, 161.2, 156.1, 151.7, 146.9, 138.9, 134.3, 134.0, 132.5, 129.1, 128.7,
128.4, 128.1, 127.4, 126.6, 103.6, 93.3, 81.7, 80.4, 78.5, 77.6, 77.2, 70.3, 69.6, 65.9, 60.3, 51.3, 51.2, 49.4, 47.5, 42.6, 42.3, 40.2, 39.6, 38.0, 36.2, 30.9, 30.5, 28.2, 22.4,
21.2, 20.3, 19.6, 15.5, 14.5, 13.1, 11.1, 10.6.
EXAMPLE 31
13 5,C NMR (CDCI3) δ 204.8, 179.7, 169.4, 156.0, 145.1, 138.8, 131.9, 128.8, 128.2, 127.0, 125.2, 122.0, 103.5, 91.0, 81.5, 80.2, 79.5, 77.3, 77.3, 70.3, 69.6, 65.9, 61.1,
60.2, 56.0, 51.4, 51.2, 49.4, 47.3, 42.7, 42.3, 42.0, 40.2,
38.0, 36.2, 28.2, 22.3, 21.2, 20.2, 19.6, 15.3, 14.5, 13.0,
11.1, 10.5.
EXAMPLE 32 13C NMR (CDC13) δ 205.3, 180.7, 169.1, 156.7, 152.1, 150.1, 146.8, 138.6, 129.2, 126.3, 123.3, 121.8, 119.5, 112.9, 103.9, 87.0, 85.5, 82.3, 80.3, 77.7, 77.6, 70.4, 69.7, 65.8, 60.5, 51.3, 51.3, 49.1, 47.5, 42.5, 42.3, 40.2,
38.2, 36.1, 28.1, 22.6, 21.2, 20.1, 19.5, 15.5, 14.7, 13.2, 11.2, 10.7.
The foregoing is merely illustrative of this invention and is not intended to limit the same to the disclosed compounds. Variations and changes which are obvious to one skilled in the art are intended to be within the scope and nature of this invention which is defined in the appended claims .

Claims

WHAT IS CLAIMED IS:
1. A compound, or salt, prodrug, or salt of a prodrug thereof, having formula (I)
Figure imgf000049_0001
(I), in which two of A 1, B1, D1, and E1 are hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, -CN, -OH, -SH, -C(0)H, -C(0)R1, -C(0)OH, -C(0)ORX, -C(0)NR2R3, or alkyl substituted by one, two, or three substituents independently selected from the group consisting of -CN, -OH, -SH, halo, aryl, heteroaryl, heterocyclyl, -OR , -SR1, -C(0)H, -C(0)R1, -C(0)OH, -C(0)OR1, -CH=N-OR1, -OC (0) R1, -OC(0)ORX, -C(0)NR2R3, -OC(0)NR2R3, -NR2R3, -N(R4)C(0)H, -N(R4)C(0)R1, -N(R )C(0)NR2R3, -N (R4) S02R1, -OR1, -SR1, -S(0)R1, -S02R1, and -S02NR2R3, and the remainder are hydrogen; or
A1 and D1, A1 and E1, B1 and D1, or B1 and D1 together are one- to five-membered alkylene or two- to five-membered heteroalkylene, and the remainder are hydrogen; or
A and B together are one- to seven-membered alkylene or two- to seven-membered heteroalkylene, and D and E are hydrogen; or
D 1 and E1 together are one- to seven-membered alkylene or two- to seven-membered heteroalkylene, and A and B are hydrogen; X is hydrogen or fluoride;
M1 is (E)-CH=CH, (Z)-CH=CH, or C≡C; ι Y is arylene or heteroarylene;
_ L is drawn from left to right and is alkylene, alkenylene, alkynylene, CH=N-0-CH2- (alkenylene) , CH2N(R5), CH2N(R5) (CH2)m, C(0)N(R5), N (R5) C (0) N (R6) , CH=N-N (R5) , CH=N-N(R5)C(0) , 0, CH=N-0, CH=N-0- (CH2) m, C (0) N (R5) (CH2) m, or CH=N-0(CH2)n-0, in which m is one, two, three, or four, and n is two, three, or four;
W is hydrogen aryl, heteroaryl, or heterocyclyl;
_ R is alkyl, aryl, heteroaryl, or heterocyclyl;
R 2 and R3 are i.ndependently hydrogen or alkyl; or R 2 and R3 together are 3- to 7-membered alkylene or 3- to 7-membered heteroalkylene; R is hydrogen or alkyl; R and R are independently hydrogen or alkyl; and
R A i•s hydrogen or R,P i■n which RP i•s a hydroxyl protecting moiety; in which, for the foregoing, each aryl, arylene, heteroaryl, heteroarylene, heterocyclyl, and heterocycloalkylene is unsubstituted or substituted by one, two, three, four, or five substituents independently selected from the group consisting of alkyl, alkenyl, alkynyl, cycloalkyl, halo, -CN, -OH, -SH, -NH2, -N02, =0, -CF3, -CH2CF3, -CF2CF3, -0CF3, -OCH2CF3, -OCF2CF3, -OR30, -SR30, -S(0)R35, -S02R35, -C(0)H, -C(0)R35, -C(0)OH, -C(0)OR35, -NH(R35), -N(R35) (R36) , -C(0)NH2, -C (0) NH (R35) , -C(0)N(R35) (R36) , -OC(0)R35, -OC(0)OR35, -OC(0)NH2,
-0C(0)NH(R35) , -0C(0)N(R35) (R36) , -NHC(0)H, -NHC(0)R35, -NHC(0)OR35, -NHC(0)NH2, -NHC (0) NH (R35) , -NHC (0) N (R35) (R36) , -S02NH2, -S02NH(R35), -S02N (R35) (R36) , R40, and alkyl substituted with one or two substituents independently selected from the group consisting of halo, -CN, -OH, -SH, =0, -OR30, -SR30, -C(0)OH, -C(0)OR35, -NH2, -NH(R35), -N(R35) (R36) , -C(0)NH2, -C(0)NH(R35) , -C (O) N (R35) (R36) , -OC(0)R35, -0C(0)NH2, -OC(0)NH(R35) , -OC (O) N (R35) (R35) ,
-S02NH2, -S02NH(R35), -S02N (R35) (R36) , ' and R40; R30 is alkyl or alkyl substituted with a substituent selected from the group consisting of halo and -OR ; R and R are independently selected alkyl; R is phenyl, naphthyl, furyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, 1, 2, 3-oxadiazolyl, 1, 2, 3-thiadiazolyl,
1, 3, 4-thiadiazolyl, 1, 2, 3-triazolyl, tetrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyrrolidinyl, inidazolidinyl, piperidinyl, piperazinyl, morpholinyl, or thiomorpholinyl, each of which is unsubstituted or substituted with one, two, or three substituents independently selected from the group consisting of alkyl, alkenyl, alkynyl, cycloalkyl, halo, -CN, -OH, -SH, -N02, =0, -CF3, -CH2CF3, -CF2CF3, -0CF3,
-OCH2CF3, -OCF2CF3, -OR45, -SR45, -S(0)R5°, -S02R5°, -C(0)H,
-C(0)R5°, -C(0)0H, -C(0)0R5°, -NH2, -NH(R50), -N (R50) (R51) , -C(0)NH2, -C(0)NH(R5°) , -C (0) N (R50) (R51) , -0C(0)R5°,
-OC(0)OR5°, -OC(0)NH2, -OC(0)NH(R50) , -OC (0) N (R50) (R51) ,
-NHC(0)H, -NHC(0)R5°, -NHC (0) OR50, -NHC(0)NH2, -NHC (0) NH (R50) ,
-NHC(0)N(R5°) (R51) , -S02NH2, S02NH(R5°), and -S02N (R50) (R51) ;
4 R is alkyl; and R and R are independently selected alkyl.
2. A compound of claim 1 in which A , B1, D1, and E1 are hydrogen; X 1 is hydrogen; M1 is CsC; Y1 is arylene or heteroarylene, in which the Y arylene is 1, 3-phenylene or 1, 4-phenylene, and in which the Y heteroarylene is 2, 5-thienylene; L is drawn from left to right and is alkynylene, CH=N-0-CH2- (alkenylene) , CH2N(R5), CH2 (R5) (CH2)m, C(0)N(R5), N(R5)C(0)N(R6) , CH=N-N (R5) , CH=N-N (R5) C (0) , 0, CH=N-0, CH=N-0(CH2)m, C (0) N (R5) (CH2) m, or CH=N-0(CH2)n-0, in which m is one, two, or three, and n is three; W is hydrogen, aryl, or heteroaryl, in which the aryl is phenyl or phenyl fused with another phenyl (naphthyl) , each of which is unsubstituted or substituted by one substituent selected from the group consisting of halo and R , in which R is 1, 2, 3-thiadiazolyl, and in which the heteroaryl is pyridyl, or pyridyl fused with phenyl (quinolinyl) ; with the proviso that W 1 is hydrogen only when L1 is CH=N-0 (CH2)m; R5 is hydrogen or alkyl; R is hydrogen; and RA is hydrogen.
3. A compound of claim 1 in which A I, Bl, Dl, and E1 are hydrogen; X 1 is hydrogen; M1 i.s C≡C; Y1 is arylene or heteroarylene, in which the arylene is 1, 3-phenylene or 1, 4-phenylene, and in which the heteroarylene is 2, 5-thienylene; L is drawn from left to right and is C2-alkynylene, CH=N-0-CH2- (C2-alkenylene) , CH2N (R5) , CH2N(R5) (CH2)m, C(0)N(R5), N (R5) C (0) N (R6) , CH=N-N (R5) , CH=N-N(R5)C(0) , 0, CH=N-0, CH=N-0 (CH2) m, C (O) N (R5) (CH2) m, or CH=N-0 (CH ) n-0, in which m is one, two, or three, and n is three; W is hydrogen, aryl, heteroaryl, or heterocyclyl, in which the aryl is phenyl or phenyl fused with another phenyl (naphthyl) , each of which is unsubstituted or substituted by one substituent selected from the group consisting of halo
40 40 . and R , m which R is 1, 2, 3-thιadιazolyl, and in which the heteroaryl is pyridyl or pyridyl fused with phenyl
(quinolinyl) ; with the proviso that W is hydrogen only when
L 1 is CH=N-0(CH2)m and m is one; R5 is hydrogen or Cχ-alkyl;
R 6 is hydrogen; and RA is hydrogen.
4. A composition for prophylaxis or treatment of bacterial infections in a fish or a mammal, the composition comprising a therapeutically effective amount of a compound of claim 1.
5. A method for prophylaxis or treatment of bacterial infections in a fish or a mammal comprising administering thereto a therapeutically effective amount of a compound of claim 1.
6. A compound of claim 1 which is
(2R, 4R,5R, 6R, 8R, 11R, 12S,19R,20R) -11-ethyl- 2,4,6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( (phenylamino) methyl) thien-2-yl) prop-2-ynyl) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
(2R,4R,5R, 6R, 8R,11R, 12S,19R,20R) -11-ethyl- 2, 4, 6,8,12, 19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( (E)- ( ( (phenylmethyl) oxy) imino)methyl) thien-2-yl) prop-2-ynyl) - 10, 13-dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-
5-yl 3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2, 4, 6, 8, 12, 19-hexamethyl-4- (3- (5- ( (E) - ( (methyloxy) imino) ethyl) thien-2-yl) prop-2-ynyl) -7,9, 14- trioxo-10, 13-dioxa-15, 18-diazatricyclo [10.6.2.0 ' ]icos- 1(18) -en-5-yl 3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl- 2,4,6,8,12,19-hexamethyl-7,9,14-trioxo-4-(3- (5-( (E)-
( (phenyloxy) imino) methyl) thien-2-yl)prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl
3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl- 2,4,6,8,12,19-hexamethyl-4- (3- (5-( (E)-( ( (naphthalen-1- ylmethyl) oxy) imino)methyl) thien-2-yl) prop-2-ynyl) -7,9, 14- trioxo-10, 13-dioxa-15, 18-diazatricyclo [10.6.2.0 ' ] icos-
1(18) -en-5-yl 3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl-
2,4,6,8,12,19-hexamethyl-4-(3-(5-( (E)-( ( (3-naphthalen-l- ylprop-2-enyl) oxy) imino)methyl) thien-2-yl) prop-2-ynyl) - 7, 9, 14-trioxo-10, 13-dioxa-15, 18- diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2,4,6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( (E) - ( ( (2- (phenyloxy) ethyl) oxy) imino)methyl) thien-2-yl) prop-2-ynyl) - 10,13-dioxa-15, 18-diazatricyclo [10.6.2.015'20] icos-1 (18) -en- 5-yl 3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl-
2, 4, 6, 8,12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (5-
( ( (phenylmethyl ) amino) methyl) thien-2-yl) prop-2-ynyl ) -10 , 13- dioxa-15 , 18-diazatricyclo [ 10 . 6 . 2 . o15'20] icos-1 ( 18 ) -en-5-yl
3 , 4 , 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
( 2R, 4R, 5R, 6R, 8R, 11R, 12S , 19R, 20R) -11-ethyl- 2 , 4 , 6 , 8 , 12 , 19-hexamethyl-7 , 9, 14-trioxo-4- ( 3- ( 5- ( (E) - (pyridin-2-ylhydrazono) methyl) thien-2-yl)prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl
3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, N'- ( (IE) - (5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2 OR) -11- ethyl-2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-5- ( (3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) - 10 , 13-dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 ( 18) -en- 4-yl) prop-1-ynyl) thien-2-yl)methylidene) pyridine-2- carbohydrazide,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl-
2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( (E) - (( (quinolin-3-ylmethyl) oxy) imino)methyl) thien-2-yl) rop-2- ynyl) -10, 13-dioxa-15, 18-diazatricyclo [10.6.2. o15,20] icos-
1(18) -en-5-yl 3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo- hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- ( ( (2- phenylethyl) amino)methyl) thien-2-yl) prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-5-yl
3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl- 2,4,6,8,12,19-hexamethyl-7,9,14-trioxo-4-(3-(5-( ( (3- phenylpropyl) amino)methyl) thien-2-yl) prop-2-ynyl) -10, 13- dioxa-15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl
3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
(2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2, 4, 6,8, 12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (3- (pyridin-2- yloxy) phenyl) prop-2-ynyl) -10, 13-dioxa-15, 18- diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
5- (3- ( (2R, 4R,5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl- 2, 4, 6,8,12,19-hexamethyl-7, 9, 14-trioxo-5- ( (3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-4-yl) prop-1- ynyl) -N- (3-f luorophenyl) thiophene-2-carboxamide,
5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl-
85 2, 4, 6, 8, 12,19-hexamethyl-7,9,14-trioxo-5- ( (3,4, 6-trideoxy-3- ( dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-4-yl) prop-1- ynyl) -N- (3-f luorophenyl) -N-methylthiophene-2-carboxamide,
(2R,4R,5R, 6R,8R, 11R, 12 S, 19R, 2 OR) -ll-ethyl-4- (3- (3- ( (3-
90 fluorophenyl) oxy) phenyl) prop-2-ynyl) -2,4, 6, 8, 12, 19- hexamethyl-7, 9, 14-trioxo-10, 13-dioxa-15, 18- diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 95 2, 4, 6, 8, 12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (5- (pyridin-2- ylethynyl) thien-2-yl) prop-2-ynyl) -10, 13-dioxa-15, 18- diazatricyclo [10.6.2.015,20]icos-l(18)-en-5-yl 3,4, 6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl- 100 2,4,6,8, 12,19-hexamethyl-7, 9, 14-trioxo-4- (3- (4-
(phenyloxy) phenyl) prop-2-ynyl) -10, 13-dioxa-15, 18- diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl 3,4,6- trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside,
5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 105 2,4,6, 8,12,19-hexamethyl-7,9,14-trioxo-5-( (3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2.0 ' ] icos-1 (18) -en-4-yl) prop-1- ynyl) -N-pyridin-3-ylthiophene-2-carboxamide,
5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 110 2,4,6, 8,12,19-hexamethyl-7,9,14-trioxo-5-( (3,4, 6-trideoxy-3-
( dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-4-yl) prop-1- ynyl) -N- (4- (1,2, 3-thiadiazol-4-yl) phenyl) thiophene-2- carboxamide,
115 5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl-
2, 4, 6, 8, 12, 19-hexamethyl-7, 9, 14-trioxo-5- ((3,4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2.015'20] icos-1 (18) -en- -yl) prop-1- ynyl) -N- (3-quinolin-3-ylpropyl) thiophene-2-carboxamide,
120 (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 2OR) -11-ethyl- 2,4, 6,8,12,19-hexamethyl-4- (3- (5-
( (methyl (phenylmethyl) amino)methyl) thien-2-yl) prop-2-ynyl) - 7, 9,14-trioxo-10, 13-dioxa-15, 18- diazatricyclo [10.6.2. o15'20] icos-1 (18) -en-5-yl 3,4,6-
125 trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranoside, or
N- (5- (3- ( (2R, 4R, 5R, 6R, 8R, 11R, 12S, 19R, 20R) -11-ethyl- 2, 4, 6,8,12,19-hexamethyl-7, 9, 14-trioxo-5- ( (3, 4, 6-trideoxy-3- (dimethylamino) -β-D-xylo-hexopyranosyl) oxy) -10, 13-dioxa- 15, 18-diazatricyclo [10.6.2. o15'20] icos-1 (18 ) -en-4-yl) prop-1-
'130 ynyl) thien-2-yl) -N' -pyridin-4-ylurea.
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