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WO2002035219A2 - Dispositif de mesure permettant de detecter une distribution monodimensionnelle ou multidimensionnelle d'un composant chimique ou biochimique - Google Patents

Dispositif de mesure permettant de detecter une distribution monodimensionnelle ou multidimensionnelle d'un composant chimique ou biochimique Download PDF

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Publication number
WO2002035219A2
WO2002035219A2 PCT/DE2001/003980 DE0103980W WO0235219A2 WO 2002035219 A2 WO2002035219 A2 WO 2002035219A2 DE 0103980 W DE0103980 W DE 0103980W WO 0235219 A2 WO0235219 A2 WO 0235219A2
Authority
WO
WIPO (PCT)
Prior art keywords
photosensitive
contact
measuring arrangement
layer
electrode
Prior art date
Application number
PCT/DE2001/003980
Other languages
German (de)
English (en)
Other versions
WO2002035219A3 (fr
Inventor
Michael Josef SCHÖNING
Arshak Poghossian
Hans LÜTH
Tatsuo Yoshinobu
Hiroshi Iwasaki
Original Assignee
Forschungszentrum Jülich GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forschungszentrum Jülich GmbH filed Critical Forschungszentrum Jülich GmbH
Priority to EP01988860A priority Critical patent/EP1337842A2/fr
Publication of WO2002035219A2 publication Critical patent/WO2002035219A2/fr
Publication of WO2002035219A3 publication Critical patent/WO2002035219A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/002—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the work function voltage

Definitions

  • the invention relates to a measuring arrangement for the detection of a chemical or biological component, in particular for the one- or multi-dimensional distribution of the component to be detected.
  • a disadvantage of such arrangements is that with a two-dimensional local resolution, a large number of leads, bond pads and external leads are required, which on the one hand limits the real local resolution and on the other hand makes the downstream electronics enormous complicated. For example, multipotentiostats for such electrode arrays are still not commercially available today. The active sensor area thus remains limited by the number of electrodes.
  • Japanese patent application JP 07335956 (Optical scanning two-dimensional sensor, 1995) describes an optical sensor with which two-dimensional scanning of a surface is possible.
  • This sensor consists of a transparent first electrode and a photoconductive (photon-conductive) layer which is in contact with the surface of this electrode and which only experiences a change in conductivity in the area which is illuminated.
  • the sensor comprises a functionalized layer, which is located on the photoconductive layer, and a second electrode, which is located away from the functionalized layer.
  • the sensor arrangement of this amperometric measuring arrangement consists of inseiform metal electrodes in the form of metallic areas on the top of the photoconductive layer.
  • the individual electrodes in-shaped metal electrodes
  • the sensor area is not limited by the number of electrodes. The individual electrodes are addressed via the scanned light or laser beam.
  • the local resolution is also limited by the fact that the ratio of the illuminated area to the total sensor area must be greater than the ratio of the conductivities in the illuminated and dark state.
  • Another disadvantage is the relatively slow switchover time in the millisecond range, which is often not sufficient, especially for fast chemical reactions, for example for applications in electrophysiology.
  • the first electrode must be transparent to practice this measurement principle. This severely limits the selection of materials that can be used, especially when considering sensor technology-compatible embodiments of the sensor.
  • the electrodes proposed in the Japanese patent application are slightly smaller in diameter than the laser beam. This regularly limits the use of electrodes of different sizes and limits the use of lasers with different wavelengths.
  • the object of the invention is to provide a measuring arrangement for the detection of a chemical or biological component which enables a one- or two-dimensional distribution of the component to be detected and which does not have the disadvantages described in the prior art. Furthermore, it is an object of the invention to provide a corresponding measurement method for the detection of a one- or multi-dimensional distribution of a chemical or biological component.
  • the measuring arrangement according to claim 1 comprises a first electrode and a substrate which is in direct contact with the first electrode. Furthermore, the measuring arrangement comprises one or more photosensitive structures which are formed on or / and in the substrate. Each of these photosensitive structures comprises at least one photosensitive contact. In the event of light, the height of the potential barrier advantageously changes with this contact.
  • the measuring arrangement according to the invention further comprises a passivation layer for isolating the photosensitive structures and the substrate. A functionalized layer is in direct contact with the surface of the photosensitive structure.
  • Electrode has no direct contact with this functionalized layer.
  • the photosensitive structure consists of at least one photosensitive contact. Any contact in which the height of the contact barrier changes under the lighting can be used as a photosensitive contact.
  • the photosensitive contact can be used as a metal-semiconductor contact (e.g. Schottky barrier, Schottky diode), pn junction, pin diode, avalanche diode etc. or as a heterocontact (e.g. semiconductor semiconductor, ion conductor Semiconductors, metal oxide semiconductors, polymer Semiconductors, biomaterial semiconductors, cell semiconductors, etc.).
  • the functionalized layer also called transducer layer, contains a (bio) / chemically sensitive material (e.g. ion, immuno, enzyme, gas, liquid sensitive). It is in direct contact with the species to be detected.
  • the properties of the functionalized layer change, for example, ions or bio-elements. It can consist of one or more layers, such as. B. metal layers or electrodes, membrane-covered layers, enzyme-covered layers, which are usually used in chemical and biosensor technology.
  • the surface or upper layer of the photosensitive structures can advantageously also be formed as a functionalized layer.
  • the passivation layer isolating the photosensitive structures comprises materials as are known as passivation materials from the prior art. These include in particular polyimides, epoxy resins, Si0 2 , Si 3 N 4 or Al 2 0 3 .
  • Si, GaAs or InSb are particularly suitable as materials for the substrate.
  • the measuring arrangement according to the invention has the following advantages over the prior art: an improved signal-to-noise ratio and thus a better detection sensitivity due to the lower dark current;
  • the external illumination of the sensor can take place from all sides, with the minimal local one when illuminated from above. Resolution can be achieved by the transducer layer or electrode geometry and / or by the contact geometry to less than 1 ⁇ xvi 2 ;
  • the arrangement can also be used as an actuator, for example for generating ions or gases.
  • the method according to the invention for one- or two-dimensional detection of an analyte substance according to claim 6 is carried out with a measuring arrangement.
  • This includes photosensitive contacts in contact with a functionalized layer, the properties of which change on contact with the analyte substance.
  • a voltage (reverse voltage) is applied between two electrodes, the photosensitive contact being in the blocked region.
  • the level of the potential barrier in the photosensitive contact then changes. This has a direct change in the current in the illuminated
  • the change in the current can be detected as a measure of the analyte substance to be detected.
  • a one- or multi-dimensional distribution of the species to be detected is advantageously achieved if the Substrate surface is illuminated and scanned in a targeted and defined manner under illumination. This can be done in a more suitable manner by means of a punctiform light beam or also suitably by means of a laser beam.
  • a further application of the measuring arrangement according to the invention can also be used advantageously for spatially resolved deposition of metal.
  • the blocking function of the photosensitive contacts is used in a suitable manner to enable current flow and thus metallic deposition at defined points (eg illuminated points).
  • Figure 1 illustrates the structure of an arrangement as it can be used to measure different substances to be detected in an analyte.
  • the measuring arrangement consists of a first electrode 1, a substrate 2, which is in direct contact with the first electrode 1 or is itself designed as an electrode.
  • the passivation continues to function as the electrical insulation of 1 and / or 2.
  • the functionalized layer (transducer layer) 5 is on the one hand in direct contact with 3 and on the other hand in direct contact with the species to be detected 6.
  • the second electrode or electrode arrangement 7 is located away from 5, but in direct contact with 6
  • a voltage reverse voltage
  • the height of the potential barrier in the photosensitive contact 3 then changes. This then results in a direct change in the current only in the illuminated area through the chemical or biochemical reaction at the interface to the transducer layer 5.
  • a one-dimensional or multidimensional distribution of the species 6 to be detected can be achieved if the surface of the arrangement is scanned in a targeted and defined manner under illumination.
  • the materials that can be used for the different layers are listed in the description above.
  • FIG. 2 corresponds to the arrangement from FIG. 1.
  • the photosensitive structure or surface 3 instead of the transducer layer 5 additionally present in FIG. 1, the photosensitive structure or surface 3 itself is designed as a transducer layer 5.
  • the materials used for this transducer layer 5 correspond to those listed in Example 1.
  • FIG. 3 corresponds in structure to the arrangement from FIG. 1, one or more pn junctions 8 being located on or in the substrate 2 as the photosensitive structure 3.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne un dispositif de mesure permettant de déceler, de manière monodimensionnelle ou bidimensionnelle, des composants chimiques ou biologiques (analytes), dispositif dans lequel un contact photosensible est appliqué sur une structure à semi-conducteur, et la variation de barrière de potentiel de contact lors de l'éclairage est utilisée comme principe de fonctionnement. Le dispositif selon l'invention permet ainsi d'éviter tous les inconvénients que l'on relève dans l'état de la technique, tout en garantissant néanmoins, au moyen d'un détecteur, la résolution locale bidimensionnelle de la substance analyte à détecter.
PCT/DE2001/003980 2000-10-24 2001-10-23 Dispositif de mesure permettant de detecter une distribution monodimensionnelle ou multidimensionnelle d'un composant chimique ou biochimique WO2002035219A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP01988860A EP1337842A2 (fr) 2000-10-24 2001-10-23 Dispositif de mesure permettant de detecter une distribution monodimensionnelle ou multidimensionnelle d'un composant chimique ou biochimique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2000152670 DE10052670A1 (de) 2000-10-24 2000-10-24 Meßanordnung zum Nachweis einer ein- oder mehrdimensionalen Verteilung einer chemischen oder biochemischen Komponente
DE10052670.5 2000-10-24

Publications (2)

Publication Number Publication Date
WO2002035219A2 true WO2002035219A2 (fr) 2002-05-02
WO2002035219A3 WO2002035219A3 (fr) 2002-12-19

Family

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PCT/DE2001/003980 WO2002035219A2 (fr) 2000-10-24 2001-10-23 Dispositif de mesure permettant de detecter une distribution monodimensionnelle ou multidimensionnelle d'un composant chimique ou biochimique

Country Status (3)

Country Link
EP (1) EP1337842A2 (fr)
DE (1) DE10052670A1 (fr)
WO (1) WO2002035219A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6598971B2 (en) 2001-11-08 2003-07-29 Lc Technologies, Inc. Method and system for accommodating pupil non-concentricity in eyetracker systems

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995014962A1 (fr) * 1993-11-25 1995-06-01 Technobiochip Biocapteurs potentiometriques, systeme de commande de ces derniers et leurs applications
JP2000111515A (ja) * 1998-10-02 2000-04-21 Horiba Ltd 二次元化学画像測定装置
DE19840157A1 (de) * 1998-09-03 2000-06-15 Axel Lorke Ortsaufgelöster Potential-Sensor und -Stimulator auf Halbleiterbasis

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Publication number Priority date Publication date Assignee Title
US5024223A (en) * 1989-08-08 1991-06-18 Chow Alan Y Artificial retina device
JP3054740B2 (ja) * 1990-06-12 2000-06-19 新電元工業株式会社 生物化学センサ
DE19529371C3 (de) * 1995-08-10 2003-05-28 Nmi Univ Tuebingen Mikroelektroden-Anordnung
JPH09210958A (ja) * 1995-11-29 1997-08-15 Horiba Ltd 光走査型二次元センサ

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995014962A1 (fr) * 1993-11-25 1995-06-01 Technobiochip Biocapteurs potentiometriques, systeme de commande de ces derniers et leurs applications
DE19840157A1 (de) * 1998-09-03 2000-06-15 Axel Lorke Ortsaufgelöster Potential-Sensor und -Stimulator auf Halbleiterbasis
JP2000111515A (ja) * 1998-10-02 2000-04-21 Horiba Ltd 二次元化学画像測定装置

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
HAFEMAN D G ET AL: "LIGHT-ADDRESSABLE POTENTIOMETRIC SENSOR FOR BIOCHEMICAL SYSTEMS" SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, Bd. 240, 27. Mai 1988 (1988-05-27), Seiten 1182-1185, XP000609230 ISSN: 0036-8075 *
PATENT ABSTRACTS OF JAPAN vol. 016, no. 231 (P-1361), 28. Mai 1992 (1992-05-28) & JP 04 050648 A (SHINDENGEN ELECTRIC MFG CO LTD), 19. Februar 1992 (1992-02-19) *
PATENT ABSTRACTS OF JAPAN vol. 1997, no. 12, 25. Dezember 1997 (1997-12-25) & JP 09 210958 A (HORIBA LTD), 15. August 1997 (1997-08-15) *
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 07, 29. September 2000 (2000-09-29) & JP 2000 111515 A (HORIBA LTD), 21. April 2000 (2000-04-21) *
SEKI ET AL.: "Novel sensors for potassium, calcium and magnesium ions based on a silicon transducer as a light-addressable potentiometric sensor" ANALYTICA CHIMICA ACTA, Bd. 382, 23. Februar 1999 (1999-02-23), Seiten 131-136, XP002216685 *
YOSHINOBU ET AL.: "Alternative sensor materials for light-addressable potentiometric sensors" SENSORS AND ACTUATORS B, Bd. 76, 1. Juni 2001 (2001-06-01), Seiten 388-392, XP002216686 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6598971B2 (en) 2001-11-08 2003-07-29 Lc Technologies, Inc. Method and system for accommodating pupil non-concentricity in eyetracker systems

Also Published As

Publication number Publication date
WO2002035219A3 (fr) 2002-12-19
DE10052670A1 (de) 2002-05-08
EP1337842A2 (fr) 2003-08-27

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