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WO2002016635A3 - Verfahren zur markierung chemischer substanzen - Google Patents

Verfahren zur markierung chemischer substanzen Download PDF

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Publication number
WO2002016635A3
WO2002016635A3 PCT/DE2001/003009 DE0103009W WO0216635A3 WO 2002016635 A3 WO2002016635 A3 WO 2002016635A3 DE 0103009 W DE0103009 W DE 0103009W WO 0216635 A3 WO0216635 A3 WO 0216635A3
Authority
WO
WIPO (PCT)
Prior art keywords
chemical substances
receptor
marking
nucleic acid
chemical substance
Prior art date
Application number
PCT/DE2001/003009
Other languages
English (en)
French (fr)
Other versions
WO2002016635A2 (de
Inventor
Gerhard Hartwich
Michael Bandilla
Original Assignee
Friz Biochem Gmbh
Gerhard Hartwich
Michael Bandilla
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Friz Biochem Gmbh, Gerhard Hartwich, Michael Bandilla filed Critical Friz Biochem Gmbh
Priority to EP01971604A priority Critical patent/EP1334208A2/de
Publication of WO2002016635A2 publication Critical patent/WO2002016635A2/de
Publication of WO2002016635A3 publication Critical patent/WO2002016635A3/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Es wird ein Verfahren zur Markierung und zur Detektion von chemischen Substanzen beschrieben, die spezifisch an einen Rezeptor binden. Dabei dient die Sequenz eines DNA-, RNA- oder PNA-Oligomer-Einzelstrangs als eindeutige molekulare Markierung, die am jeweiligen Rezeptor angebracht wird. Nach Abtrennung der Komplexe aus chemischer Substanz und Nukleinsäureoligomer-Rezeptor-Einheit wird statt der chemischen Substanz der Nukleinsäureoligomer-Bestandteil identifiziert und quantifiziert.
PCT/DE2001/003009 2000-08-25 2001-08-04 Verfahren zur markierung chemischer substanzen WO2002016635A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP01971604A EP1334208A2 (de) 2000-08-25 2001-08-04 Verfahren zur markierung chemischer substanzen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2000141766 DE10041766A1 (de) 2000-08-25 2000-08-25 Verfahren zur Markierung chemischer Substanzen
DE10041766.3 2000-08-25

Publications (2)

Publication Number Publication Date
WO2002016635A2 WO2002016635A2 (de) 2002-02-28
WO2002016635A3 true WO2002016635A3 (de) 2003-06-05

Family

ID=7653758

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/003009 WO2002016635A2 (de) 2000-08-25 2001-08-04 Verfahren zur markierung chemischer substanzen

Country Status (3)

Country Link
EP (1) EP1334208A2 (de)
DE (1) DE10041766A1 (de)
WO (1) WO2002016635A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10156329A1 (de) * 2001-07-17 2003-02-06 Frieder Breitling Verfahren und Anordnung zum Anbringen von in Transportmittel immobilisierten Substanzen sowie Monomerpartikel
DE10200374A1 (de) * 2002-01-08 2003-07-24 Friz Biochem Gmbh Verfahren zur qualitativ und quantitativ vergleichenden Detektion chemischer Substanzen
US7341837B2 (en) * 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
WO2006002382A2 (en) * 2004-06-24 2006-01-05 The Scripps Research Institute Arrays with cleavable linkers
CZ2014527A3 (cs) * 2014-08-05 2016-02-17 Ústav Organické Chemie A Biochemie Akademie Věd Čr, V.V.I. Způsob detekce aktivní formy analytů ve vzorku a stanovení schopnosti dalších látek vázat se do aktivních míst těchto analytů
CN112834736B (zh) * 2020-12-02 2024-09-06 杭州百凌生物科技有限公司 一种检测多种抗原的病理检测试剂盒及其制备方法和应用
CN115436622B (zh) * 2022-09-26 2024-03-08 重庆医科大学国际体外诊断研究院 一种单分子蛋白的检测方法及其试剂盒和应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0314904A1 (de) * 1987-09-24 1989-05-10 MERCK PATENT GmbH Verfahren und Reagenz zur Durchführung von Hybridisierungsreaktionen
EP0530998A1 (de) * 1991-08-15 1993-03-10 Microgenics Corporation Nachweis von komplementären Nukleotid-Sequenzen
WO1996032640A1 (en) * 1995-04-04 1996-10-17 Chiron Diagnostics Corporation Sensitive immunoassays utilizing antibody conjugates with replicable dna templates
US5665539A (en) * 1991-07-12 1997-09-09 The Regents Of The University Of California Immuno-polymerase chain reaction system for antigen detection
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
WO1999063109A1 (de) * 1998-06-04 1999-12-09 Baxter Aktiengesellschaft Verfahren zur bestimmung von antigenen

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4748111A (en) * 1984-03-12 1988-05-31 Molecular Diagnostics, Inc. Nucleic acid-protein conjugate used in immunoassay
US5635602A (en) * 1993-08-13 1997-06-03 The Regents Of The University Of California Design and synthesis of bispecific DNA-antibody conjugates
WO2000072869A1 (en) * 1999-06-01 2000-12-07 Phylos, Inc. Methods for producing 5'-nucleic acid-protein conjugates

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0314904A1 (de) * 1987-09-24 1989-05-10 MERCK PATENT GmbH Verfahren und Reagenz zur Durchführung von Hybridisierungsreaktionen
US5665539A (en) * 1991-07-12 1997-09-09 The Regents Of The University Of California Immuno-polymerase chain reaction system for antigen detection
EP0530998A1 (de) * 1991-08-15 1993-03-10 Microgenics Corporation Nachweis von komplementären Nukleotid-Sequenzen
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
WO1996032640A1 (en) * 1995-04-04 1996-10-17 Chiron Diagnostics Corporation Sensitive immunoassays utilizing antibody conjugates with replicable dna templates
WO1999063109A1 (de) * 1998-06-04 1999-12-09 Baxter Aktiengesellschaft Verfahren zur bestimmung von antigenen

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ASLAM, MOHAMMED: "Bioconjugation", 1998, NYCOMED AMERSHAM, LONDON, UK *
CASE MARIAN C ET AL: "Enhanced ultrasensitive detection of structurally diverse antigens using a single immuno-PCR assay protocol.", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 223, no. 1, 1 February 1999 (1999-02-01), pages 93 - 106, XP002234679, ISSN: 0022-1759 *
CHEMICAL ABSTRACTS, vol. 125, no. 25, 1996, Columbus, Ohio, US; abstract no. 318836z, VARY, CALVIN P.H.: "Nonisotopic DNA labelling strategies" XP008014182 *
CHEMICAL ABSTRACTS, vol. 130, no. 19, 1998, Columbus, Ohio, US; abstract no. 248388z, SIMMONDS, A., CUNNINGHAM, M.: "The preparation of protein-nucleic acid conjugates" XP008014161 *
DANGLER, CHARLES A.: "Nucleic Acid Analysis", 1996, WILEY, NEW YORK, N.Y. *
HENDRICKSON EDWIN R ET AL: "High sensitivity multianalyte immunoassay using covalent DNA-labeled antibodies and polymerase chain reaction", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 23, no. 3, 1995, pages 522 - 529, XP002151883, ISSN: 0305-1048 *
JOERGER R D ET AL: "ANALYTE DETECTION WITH DNA-LABELED ANTIBODIES AND POLYMERASE CHAIN REACTION", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY. WINSTON, US, vol. 41, no. 9, September 1995 (1995-09-01), pages 1371 - 1377, XP001018334, ISSN: 0009-9147 *
NIEMEYER CHRISTOF M ET AL: "Self-assembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR.", NUCLEIC ACIDS RESEARCH, vol. 27, no. 23, 1 December 1999 (1999-12-01), pages 4553 - 4561, XP002176567, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2002016635A2 (de) 2002-02-28
EP1334208A2 (de) 2003-08-13
DE10041766A1 (de) 2002-03-14

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