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WO2002004660A3 - Procede permettant de tester l'inhibition des isozymes cytochromes p450 a metabolisation de medicament - Google Patents

Procede permettant de tester l'inhibition des isozymes cytochromes p450 a metabolisation de medicament Download PDF

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Publication number
WO2002004660A3
WO2002004660A3 PCT/US2001/021909 US0121909W WO0204660A3 WO 2002004660 A3 WO2002004660 A3 WO 2002004660A3 US 0121909 W US0121909 W US 0121909W WO 0204660 A3 WO0204660 A3 WO 0204660A3
Authority
WO
WIPO (PCT)
Prior art keywords
isozymes
inhibition
drug
testing
metabolizing cytochrome
Prior art date
Application number
PCT/US2001/021909
Other languages
English (en)
Other versions
WO2002004660A2 (fr
Inventor
Elizabeth A Dierks
Heng-Keang Lim
Simon Edward Ball
Original Assignee
Wyeth Corp
Elizabeth A Dierks
Heng-Keang Lim
Simon Edward Ball
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp, Elizabeth A Dierks, Heng-Keang Lim, Simon Edward Ball filed Critical Wyeth Corp
Priority to AU2001271985A priority Critical patent/AU2001271985A1/en
Publication of WO2002004660A2 publication Critical patent/WO2002004660A2/fr
Publication of WO2002004660A3 publication Critical patent/WO2002004660A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/007—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isoenzyme profiles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé permettant de tester simultanément l'inhibition ou l'induction/activation, par un certain nombre de médicaments candidats potentiels, des isozymes cytochromes P450 (CYP450) à métabolisation de médicament, du type CYP3A4, CYP2D6, CYP2C9, CYP1A2, CYP2C19, CYP2A6, et CYP2C8, en utilisant un mélange de substrats de sondes spécifiques pour les isozymes CYP450. L'invention concerne également un procédé qui permet de déterminer le phénotype d'un échantillon de cellule ou de tissu en déterminant le ou les isozymes CYP450 présents dans l'échantillon de cellule ou de tissu, sur la base du procédé décrit.
PCT/US2001/021909 2000-07-12 2001-07-11 Procede permettant de tester l'inhibition des isozymes cytochromes p450 a metabolisation de medicament WO2002004660A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001271985A AU2001271985A1 (en) 2000-07-12 2001-07-11 Method for testing for inhibition of drug-metabolizing cytochrome p450 isozymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21854700P 2000-07-12 2000-07-12
US60/218,547 2000-07-12

Publications (2)

Publication Number Publication Date
WO2002004660A2 WO2002004660A2 (fr) 2002-01-17
WO2002004660A3 true WO2002004660A3 (fr) 2003-02-27

Family

ID=22815538

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/021909 WO2002004660A2 (fr) 2000-07-12 2001-07-11 Procede permettant de tester l'inhibition des isozymes cytochromes p450 a metabolisation de medicament

Country Status (2)

Country Link
AU (1) AU2001271985A1 (fr)
WO (1) WO2002004660A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105241992A (zh) * 2015-09-30 2016-01-13 成都华西海圻医药科技有限公司 一种肝微粒体中4’-羟基双氯芬酸浓度的uplc/ms/ms检测方法

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US6489094B2 (en) * 2000-05-31 2002-12-03 Pfizer Inc. Method and device for drug-drug interaction testing sample preparation
EP1607479B1 (fr) * 2003-03-04 2013-05-01 Hideko Kanazawa Systeme d'evaluation de la capacite metabolique pour un medicament et methode d'utilisation dudit systeme
RU2316327C1 (ru) * 2006-10-05 2008-02-10 Институт органической и физической химии им. А.Е. Арбузова Казанского научного центра РАН (ИОФХ им. А.Е. Арбузова КазНЦ РАН) Ксимедон в качестве индуктора активности микросомальных оксидаз печени человека
CN105136962A (zh) * 2015-09-30 2015-12-09 成都华西海圻医药科技有限公司 一种肝微粒体中1-羟基咪达唑仑浓度的uplc/ms/ms检测方法
CN105277635A (zh) * 2015-09-30 2016-01-27 成都华西海圻医药科技有限公司 一种肝微粒体中右啡烷浓度的uplc/ms/ms检测方法
CN110713921B (zh) * 2018-07-13 2022-12-20 复旦大学 一套体外动态的药物代谢孵育系统
CN109596752A (zh) * 2019-02-01 2019-04-09 重庆市农业科学院 一种高效液相色谱串联质谱法检测蚯蚓体内cyp1a2和cyp3a4酶活力的方法
CN113720944B (zh) * 2021-09-30 2023-04-28 珠海润都制药股份有限公司 一种氢溴酸右美沙芬硫酸奎尼丁胶囊含量的检测方法
WO2024223797A1 (fr) 2023-04-28 2024-10-31 Institut National de la Santé et de la Recherche Médicale Utilisation d'inhibiteurs de cyp3a4 pour le traitement d'infections par le virus de l'hépatite d (vhd)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0626453A1 (fr) * 1993-05-25 1994-11-30 American Cyanamid Company Screening de cytochrome P-450 réductase pour les inhibiteurs de la biosynthèse d'ergostérol
WO1999058710A1 (fr) * 1998-05-08 1999-11-18 Smithkline Beecham Plc 7-alcoxycoumarines tenant lieu de substrats pour l'enzyme cytochrome p-450
WO2000004008A1 (fr) * 1998-07-16 2000-01-27 Gentest Corporation Nouveaux reactifs fluorescents cyp2d a essayer
US6060253A (en) * 1997-01-23 2000-05-09 The United States Of America As Represented By The Department Of Health And Human Services Agents that bind to and inhibit human cytochrome P450 2D6
WO2000035900A1 (fr) * 1998-12-14 2000-06-22 Aurora Biosciences Corporation Capteurs moleculaires optiques pour l'activite du cytochrome p450

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0626453A1 (fr) * 1993-05-25 1994-11-30 American Cyanamid Company Screening de cytochrome P-450 réductase pour les inhibiteurs de la biosynthèse d'ergostérol
US6060253A (en) * 1997-01-23 2000-05-09 The United States Of America As Represented By The Department Of Health And Human Services Agents that bind to and inhibit human cytochrome P450 2D6
WO1999058710A1 (fr) * 1998-05-08 1999-11-18 Smithkline Beecham Plc 7-alcoxycoumarines tenant lieu de substrats pour l'enzyme cytochrome p-450
WO2000004008A1 (fr) * 1998-07-16 2000-01-27 Gentest Corporation Nouveaux reactifs fluorescents cyp2d a essayer
WO2000035900A1 (fr) * 1998-12-14 2000-06-22 Aurora Biosciences Corporation Capteurs moleculaires optiques pour l'activite du cytochrome p450

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, (JUNE 15, 2000) VOL. 378, NO. 2, PP. 356-363. PRINT. *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; TASSANEEYAKUL, WICHITTRA ET AL: "Inhibition selectivity of grapefruit juice components on human cytochromes P450.", XP002215267, retrieved from STN Database accession no. 2000:334401 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105241992A (zh) * 2015-09-30 2016-01-13 成都华西海圻医药科技有限公司 一种肝微粒体中4’-羟基双氯芬酸浓度的uplc/ms/ms检测方法

Also Published As

Publication number Publication date
WO2002004660A2 (fr) 2002-01-17
AU2001271985A1 (en) 2002-01-21

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