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WO2002004660A3 - Method for testing for inhibition of drug-metabolizing cytochrome p450 isozymes - Google Patents

Method for testing for inhibition of drug-metabolizing cytochrome p450 isozymes Download PDF

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Publication number
WO2002004660A3
WO2002004660A3 PCT/US2001/021909 US0121909W WO0204660A3 WO 2002004660 A3 WO2002004660 A3 WO 2002004660A3 US 0121909 W US0121909 W US 0121909W WO 0204660 A3 WO0204660 A3 WO 0204660A3
Authority
WO
WIPO (PCT)
Prior art keywords
isozymes
inhibition
drug
testing
metabolizing cytochrome
Prior art date
Application number
PCT/US2001/021909
Other languages
French (fr)
Other versions
WO2002004660A2 (en
Inventor
Elizabeth A Dierks
Heng-Keang Lim
Simon Edward Ball
Original Assignee
Wyeth Corp
Elizabeth A Dierks
Heng-Keang Lim
Simon Edward Ball
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp, Elizabeth A Dierks, Heng-Keang Lim, Simon Edward Ball filed Critical Wyeth Corp
Priority to AU2001271985A priority Critical patent/AU2001271985A1/en
Publication of WO2002004660A2 publication Critical patent/WO2002004660A2/en
Publication of WO2002004660A3 publication Critical patent/WO2002004660A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/007—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isoenzyme profiles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for the simultaneous testing of the inhibition or induction/activation of major human drug-metabolizing cytochrome P450 isozymes, such as CYP3A4, CYP2D6, CYP2C9, CYP1A2, CYP2C19, CYP2A6, and CYP2C8, by potential drug candidates utilizing a cocktail of specific probe substrates for CYP450 isozymes is described. Also described is a method for determining the phenotype of a cell or a tissue sample by determining the CYP450 isozyme(s) present in the cell or tissue sample using the method of the invention.
PCT/US2001/021909 2000-07-12 2001-07-11 Method for testing for inhibition of drug-metabolizing cytochrome p450 isozymes WO2002004660A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001271985A AU2001271985A1 (en) 2000-07-12 2001-07-11 Method for testing for inhibition of drug-metabolizing cytochrome p450 isozymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21854700P 2000-07-12 2000-07-12
US60/218,547 2000-07-12

Publications (2)

Publication Number Publication Date
WO2002004660A2 WO2002004660A2 (en) 2002-01-17
WO2002004660A3 true WO2002004660A3 (en) 2003-02-27

Family

ID=22815538

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/021909 WO2002004660A2 (en) 2000-07-12 2001-07-11 Method for testing for inhibition of drug-metabolizing cytochrome p450 isozymes

Country Status (2)

Country Link
AU (1) AU2001271985A1 (en)
WO (1) WO2002004660A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105241992A (en) * 2015-09-30 2016-01-13 成都华西海圻医药科技有限公司 UPLC/MS/MS detection method of concentration of 4'-hydroxydiclofenac in liver microsome

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6489094B2 (en) * 2000-05-31 2002-12-03 Pfizer Inc. Method and device for drug-drug interaction testing sample preparation
EP1607479B1 (en) * 2003-03-04 2013-05-01 Hideko Kanazawa System for evaluating drug metabolism capacity and method of using the same
RU2316327C1 (en) * 2006-10-05 2008-02-10 Институт органической и физической химии им. А.Е. Арбузова Казанского научного центра РАН (ИОФХ им. А.Е. Арбузова КазНЦ РАН) Ximedon as inductor of human liver microsomal oxidase activity
CN105136962A (en) * 2015-09-30 2015-12-09 成都华西海圻医药科技有限公司 UPLC/MS/MS detection method of 1-hydroxymidazolam concentration in liver microsome
CN105277635A (en) * 2015-09-30 2016-01-27 成都华西海圻医药科技有限公司 UPLC/MS/MS detection method of dextrorphan concentration of liver microsomes
CN110713921B (en) * 2018-07-13 2022-12-20 复旦大学 In-vitro dynamic drug metabolism incubation system
CN109596752A (en) * 2019-02-01 2019-04-09 重庆市农业科学院 A kind of method that high performance liquid chromatography tandem mass spectrometry detects CYP1A2 and CYP3A4 enzyme activity in earthworm body
CN113720944B (en) * 2021-09-30 2023-04-28 珠海润都制药股份有限公司 Detection method for content of dextromethorphan hydrobromide quinidine sulfate capsules
WO2024223797A1 (en) 2023-04-28 2024-10-31 Institut National de la Santé et de la Recherche Médicale Use of cyp3a4 inhibitors for the treatment of hepatitis d virus (hdv) infections

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0626453A1 (en) * 1993-05-25 1994-11-30 American Cyanamid Company Cytochrome P-450 reductase screen for ergosterol biosynthesis inhibitors
WO1999058710A1 (en) * 1998-05-08 1999-11-18 Smithkline Beecham Plc 7-alkoxycoumarins as cytochrome p450 substrates
WO2000004008A1 (en) * 1998-07-16 2000-01-27 Gentest Corporation Novel cyp2d fluorescent assay reagents
US6060253A (en) * 1997-01-23 2000-05-09 The United States Of America As Represented By The Department Of Health And Human Services Agents that bind to and inhibit human cytochrome P450 2D6
WO2000035900A1 (en) * 1998-12-14 2000-06-22 Aurora Biosciences Corporation Optical molecular sensors for cytochrome p450 activity

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0626453A1 (en) * 1993-05-25 1994-11-30 American Cyanamid Company Cytochrome P-450 reductase screen for ergosterol biosynthesis inhibitors
US6060253A (en) * 1997-01-23 2000-05-09 The United States Of America As Represented By The Department Of Health And Human Services Agents that bind to and inhibit human cytochrome P450 2D6
WO1999058710A1 (en) * 1998-05-08 1999-11-18 Smithkline Beecham Plc 7-alkoxycoumarins as cytochrome p450 substrates
WO2000004008A1 (en) * 1998-07-16 2000-01-27 Gentest Corporation Novel cyp2d fluorescent assay reagents
WO2000035900A1 (en) * 1998-12-14 2000-06-22 Aurora Biosciences Corporation Optical molecular sensors for cytochrome p450 activity

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, (JUNE 15, 2000) VOL. 378, NO. 2, PP. 356-363. PRINT. *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; TASSANEEYAKUL, WICHITTRA ET AL: "Inhibition selectivity of grapefruit juice components on human cytochromes P450.", XP002215267, retrieved from STN Database accession no. 2000:334401 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105241992A (en) * 2015-09-30 2016-01-13 成都华西海圻医药科技有限公司 UPLC/MS/MS detection method of concentration of 4'-hydroxydiclofenac in liver microsome

Also Published As

Publication number Publication date
WO2002004660A2 (en) 2002-01-17
AU2001271985A1 (en) 2002-01-21

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