+

WO2001025779A3 - Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques - Google Patents

Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques Download PDF

Info

Publication number
WO2001025779A3
WO2001025779A3 PCT/EP2000/009777 EP0009777W WO0125779A3 WO 2001025779 A3 WO2001025779 A3 WO 2001025779A3 EP 0009777 W EP0009777 W EP 0009777W WO 0125779 A3 WO0125779 A3 WO 0125779A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
another
analysing
fluorescent markers
balanced
Prior art date
Application number
PCT/EP2000/009777
Other languages
German (de)
English (en)
Other versions
WO2001025779A2 (fr
Inventor
Wilhelm Ansorge
Josef Stegemann
Robert Ventzki
Christian Schwager
Original Assignee
Europ Lab Molekularbiolog
Wilhelm Ansorge
Josef Stegemann
Robert Ventzki
Christian Schwager
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europ Lab Molekularbiolog, Wilhelm Ansorge, Josef Stegemann, Robert Ventzki, Christian Schwager filed Critical Europ Lab Molekularbiolog
Priority to AU10219/01A priority Critical patent/AU1021901A/en
Publication of WO2001025779A2 publication Critical patent/WO2001025779A2/fr
Publication of WO2001025779A3 publication Critical patent/WO2001025779A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Dispositif d'électrophorèse (10) pour l'analyse de molécules marquées à l'aide d'un marqueur fluorescent, en particulier de molécules biologiques, qui, selon un aspect de la présente invention, est approprié pour détecter par résolution au moins quatre marqueurs fluorescents différents indépendamment les uns des autres à l'aide d'un champ détecteur attribué, selon la piste d'électrophorèse sur gel correspondante et le marqueur fluorescent correspondant. A cet effet, des filtres spectraux, relativement à leur sélectivité spectrale, les marqueurs fluorescents, relativement à leur sélectivité d'absorption et d'émission spectrale, et les longueurs d'onde des rayons laser (60, 62, 64, 66) utilisés pour l'excitation des marqueurs fluorescents sont accordés les uns par rapport aux autres de manière correspondante.
PCT/EP2000/009777 1999-10-07 2000-10-05 Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques WO2001025779A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU10219/01A AU1021901A (en) 1999-10-07 2000-10-05 Electrophoresis device for analysing labelled molecules, in particular, biological molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1999148391 DE19948391A1 (de) 1999-10-07 1999-10-07 Elektrophorese-Einrichtung zum Analysieren von markierten Molekülen, insbesondere biologischen Molekülen
DE19948391.4 1999-10-07

Publications (2)

Publication Number Publication Date
WO2001025779A2 WO2001025779A2 (fr) 2001-04-12
WO2001025779A3 true WO2001025779A3 (fr) 2002-02-28

Family

ID=7924860

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/009777 WO2001025779A2 (fr) 1999-10-07 2000-10-05 Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques

Country Status (3)

Country Link
AU (1) AU1021901A (fr)
DE (1) DE19948391A1 (fr)
WO (1) WO2001025779A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004015488B4 (de) 2004-03-26 2008-01-31 Lavision Biotec Gmbh Verfahren zum Auslesen durch streifenweises Abtasten von flächigen Objekten mit Substanzen, die Fluoreszenzstrahlung abgeben
DE102005022880B4 (de) * 2005-05-18 2010-12-30 Olympus Soft Imaging Solutions Gmbh Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern
DE102008034008B4 (de) 2008-07-21 2010-07-01 Carl Zeiss Surgical Gmbh Filtersatz zur Beobachtung von Fluoreszenzstrahlung in biologischem Gewebe
DE102008052223A1 (de) * 2008-10-17 2010-04-22 Albert-Ludwigs-Universität Freiburg Lumineszenzscanner sowie Verfahren zur Detektion von Lumineszenz in Halbleiterbauteilen
DE102016118898B4 (de) * 2016-10-05 2022-03-17 Ferdinand-Braun-Institut gGmbH, Leibniz- Institut für Höchstfrequenztechnik Optisches System und Verfahren zur Spektroskopie
CN109270004B (zh) * 2018-10-11 2023-09-12 南京信息工程大学 基于doas和libs技术的大气分析检测系统和检测方法
US20240167956A1 (en) * 2022-11-22 2024-05-23 10X Genomics, Inc. Systems and methods for imaging samples

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3046729A1 (de) * 1980-12-11 1982-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg Thermostatische platte fuer eine elektrophoreseeinrichtung
EP0251306A1 (fr) * 1986-07-04 1988-01-07 DIAGEN Institut für molekularbiologische Diagnostik GmbH Procédé et dispositif pour la préparation d'un gradient de température réglable et reproduisable ainsi que son application
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
DE19500638A1 (de) * 1994-01-14 1995-07-20 Hitachi Ltd Elektrophoresegerät
WO1996012948A1 (fr) * 1994-10-20 1996-05-02 Pharmacia Biotech Ab Procede et agencement destines a un dispositif d'electrophorese sur gel
EP0723149A2 (fr) * 1995-01-19 1996-07-24 Hitachi, Ltd. Système d'électrophorèse à réseau de capillaires
WO1996023213A1 (fr) * 1995-01-23 1996-08-01 Murray Anthony J Analyse de molecules biologiques
EP0911630A1 (fr) * 1997-08-07 1999-04-28 Hitachi Electronics Engineering Co., Ltd. Détecteur de fluorescence

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3046729A1 (de) * 1980-12-11 1982-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg Thermostatische platte fuer eine elektrophoreseeinrichtung
EP0251306A1 (fr) * 1986-07-04 1988-01-07 DIAGEN Institut für molekularbiologische Diagnostik GmbH Procédé et dispositif pour la préparation d'un gradient de température réglable et reproduisable ainsi que son application
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
DE19500638A1 (de) * 1994-01-14 1995-07-20 Hitachi Ltd Elektrophoresegerät
WO1996012948A1 (fr) * 1994-10-20 1996-05-02 Pharmacia Biotech Ab Procede et agencement destines a un dispositif d'electrophorese sur gel
EP0723149A2 (fr) * 1995-01-19 1996-07-24 Hitachi, Ltd. Système d'électrophorèse à réseau de capillaires
WO1996023213A1 (fr) * 1995-01-23 1996-08-01 Murray Anthony J Analyse de molecules biologiques
EP0911630A1 (fr) * 1997-08-07 1999-04-28 Hitachi Electronics Engineering Co., Ltd. Détecteur de fluorescence

Also Published As

Publication number Publication date
DE19948391A1 (de) 2001-04-12
AU1021901A (en) 2001-05-10
WO2001025779A2 (fr) 2001-04-12

Similar Documents

Publication Publication Date Title
ES2145580T3 (es) Metodos y composiciones para analizar moleculas de acido nucleico que emplean tecnicas basadas en el tamaño.
DE69919442D1 (de) Laserarray zur Emission von mehreren Wellenlängen
ATE179215T1 (de) 4,7-dichlorfluoreszein-farbstoffe als molekulare sonden
ATE317122T1 (de) Fluoreszierende polarisation
DE69841895D1 (de) Nukleinsäuretestverfahren
ATE409236T1 (de) Derivatisierte nukleinsäuren und deren verwendung
DK1199569T3 (da) Pröveanalysesystem
DE69634317D1 (de) Optisches Spektrometer zur Erfassung von Spektren in unterschiedlichen Bereichen
HK1090422A1 (en) A system for determining the electrical potential across a membrane
WO1999064592A3 (fr) Proteines fluorescentes vertes pour mesurer le ph d'un prelevement biologique
DE69735445D1 (de) Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
WO2000058715A3 (fr) Système de dosage
FI931109L (fi) Peptider och nukleinsyrasekvenser, som haenfoer sig till epstein-barr-virus
DE69840196D1 (de) Probenröhrchen
EP1837647A3 (fr) Système d'électrophorèse capillaire multiplexe
DE69019884D1 (de) Massenspektrometer zur Analyse von Stoffen.
WO2003019139A3 (fr) Dispositif de separation d'analyte multicanal utilisant une excitation d'entree laterale
FI961843A7 (fi) Itseosoittuvia, itserakentuvia mikroelektroniikkajärjestelmiä ja laitteita molekyylibiologista analyysiä ja diagnostiikkaa varten
FI19992092L (fi) Spektrometri ja menetelmä optisen spekterin mittaamiseksi
WO2004093129A3 (fr) Systeme de repartition de l'eclairage
CA2262739A1 (fr) Plaques a puits multiples a faible fond pour mesures de fluorescence sur echantillons biologiques et biochimiques
GB2377272A (en) Method and apparatus for DNA sequencing
DE69806367D1 (de) Rot färbende hyperchromische 3h-naphto(2,1-b)pyranen
BR9708752A (pt) Armadilha para mosca com dois ou mais fachos de luz direcional
WO2001025779A3 (fr) Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载