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WO1996012948A1 - Procede et agencement destines a un dispositif d'electrophorese sur gel - Google Patents

Procede et agencement destines a un dispositif d'electrophorese sur gel Download PDF

Info

Publication number
WO1996012948A1
WO1996012948A1 PCT/SE1995/001229 SE9501229W WO9612948A1 WO 1996012948 A1 WO1996012948 A1 WO 1996012948A1 SE 9501229 W SE9501229 W SE 9501229W WO 9612948 A1 WO9612948 A1 WO 9612948A1
Authority
WO
WIPO (PCT)
Prior art keywords
light beam
electrophoresis gel
gel
electrophoresis
incidence
Prior art date
Application number
PCT/SE1995/001229
Other languages
English (en)
Inventor
Bengt Göran ANDERSSON
Stefan Sowa
Original Assignee
Pharmacia Biotech Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biotech Ab filed Critical Pharmacia Biotech Ab
Priority to EP95936156A priority Critical patent/EP0787292A1/fr
Priority to JP8513826A priority patent/JPH10507830A/ja
Publication of WO1996012948A1 publication Critical patent/WO1996012948A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Definitions

  • the invention relates to a method and an arrangement in a gel electrophoresis apparatus having a light source whose light beam is to be introduced into an electrophoresis gel through one of its lateral edges to enable detection of at least one component which is migrating in the electrophoresis gel and which is labelled with a fluorescent substance.
  • the light source e.g. the laser
  • the object of the invention is to eliminate the manual fine adjustment mentioned above and instead bring about a gel electrophoresis apparatus in which the fine adjustment is done automatically. forth across said one lateral edge of the electrophoresis gel to introduce the light beam into the electrophoresis gel at different points of incidence, that the intensity of the light beam introduced at said different points of incidence is measured after that it, at least partially, has passed through the electrophoresis gel, and that the light beam is locked at the point of incidence at which the highest intensity is measured.
  • the object is attained by means of the arrangement according to the invention, mainly, in that a means is adapted to sweep the light beam back and forth across said one lateral edge of the electrophoresis gel to introduce the light beam into the electrophoresis gel at different points of incidence, and that a sensor is adapted to measure the intensity of the light beam introduced at said different points of incidence after that it, at least partially, has passed through the electrophoresis gel, and to lock the light beam at the point of incidence at which the highest intensity is measured.
  • FIG. 1 schematically shows a block diagram of an embodiment of an arrangement according to the invention at an electrophoresis gel, a top end view thereof being shown.
  • Fig. 1 shows a top end view of a vertical electrophoresis gel 1 between glass plates 2 and 3 which are separated by means of spacers 4 and 5 which both are translucent in the embodiment shown.
  • the shown gel 1 is intended for separation of a mixture of components, e.g. nucleic acids, and is, in a manner known per se, connected to an upper buffer liquid container (not shown) and a lower buffer liquid container (not shown) of an electrophoresis apparatus.
  • a mixture of components e.g. nucleic acids
  • the samples thereof labelled with fluorescent substances
  • the sample components migrate downwards in the gel 1 under influence of an electric field.
  • a light beam which is a laser beam 6 in the embodiment shown, is introduced through the translucent spacer 4, and into the gel 1 through one of its lateral edges in order to enable detection of the above-mentioned samples migrating in the gel 1 by means of detectors (not shown) .
  • the laser beam 6 is generated by a laser 7 and introduced into the gel 1 by means of a mirror 8.
  • a motor 9 is provided to move the mirror 8 back and forth across the lateral edge of the gel 1 as indicated by arrow 10 in order to sweep the laser beam 6 back and forth across the lateral edge of the spacer 4 and, thereby, the gel 1 as indicated by means of arrow 11 in order to introduce the laser beam 6 into the gel 1 at different points of incidence.
  • the motor 9 for moving the mirror 8 is actuated by means of a control unit 12 which is provided with a starting button (not shown) .
  • the laser beam 6 coming from the mirror 8 passes through the gel 1 and exits, in the embodiment shown, from the gel 1 through its other lateral edge via the translucent spacer 5.
  • a sensor 13 is provided at the other lateral edge of the gel 1, which sensor 13 is adapted to measure the light-intensity of the laser beam 6 during the sweeping motion of the laser beam 6, and produce a signal which is proportional to the light-intensity, and which is supplied to the control unit 12 which in its turn is adapted to stop the motor 9 and, thereby, the mirror 8 in the position for which the sensor 13 has measured the highest light-intensity of the laser beam 6.
  • the laser beam 6 is automatically set to the point of incidence for which the laser light, exiting from the gel 1, exhibits a light-intensity maximum.
  • Gel electrophoresis apparatuses are also known, in which the laser beam is introduced into the gel from the laser via a spacer without any mirror.
  • the motor corresponding to the motor 9 shown in the drawing has to be adapted to move the laser instead of the mirror in order to introduce the laser beam at different points of incidence across one of the lateral edges of the gel.
  • this embodiment (not shown) corresponds to the embodiment shown on the drawing. Measuring or sensing the light-intensity of the laser beam 6 does not necessarily have to be carried out in the manner shown on the drawing, i.e. with the sensor 13 located at the other lateral edge of the gel 1.
  • the senor 14 can e.g. be adapted to measure the intensity of the light that is scattered by the spacer 5 in case this spacer is opaque, while the sensor 15, in accordance with a further embodiment, can be adapted to measure the intensity of the light scattered in the gel 1 after that the laser beam, at least partially, has passed through the gel 1.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention se rapporte à un moyen (8, 9) utilisé dans un dispositif d'électrophorèse sur gel présentant une source lumineuse (7) dont le faisceau lumineux (6) doit être introduit dans un gel d'électrophorèse (1) à travers l'un de ses bords latéraux, permettant ainsi la détection d'au moins un constituant qui migre dans ledit gel (1) et qui est marqué par une substance fluorescente. Ce moyen (8, 9) est conçu pour déplacer le faisceau laser (6) en un mouvement alternatif de balayage sur ledit bord latéral du gel d'électrophorèse (1) et pour introduire ce faisceau dans le gel (1) au niveau de différents points d'incidence. Un capteur (13, 14, 15) est conçu pour mesurer l'intensité du faisceau lumineux (6) introduit au niveau desdits différents points d'incidence après qu'il ait traversé, au moins partiellement, le gel (1), et pour verrouiller le faisceau lumineux (6) au niveau du point d'incidence auquel l'intensité lumineuse la plus élevée a été mesurée.
PCT/SE1995/001229 1994-10-20 1995-10-19 Procede et agencement destines a un dispositif d'electrophorese sur gel WO1996012948A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP95936156A EP0787292A1 (fr) 1994-10-20 1995-10-19 Procede et agencement destines a un dispositif d'electrophorese sur gel
JP8513826A JPH10507830A (ja) 1994-10-20 1995-10-19 ゲル電気泳動装置における方法および配置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9403597A SE503503C2 (sv) 1994-10-20 1994-10-20 Förfarande och anordning för automatisk inställning av en ljusstråle vid en gelelektroforesapparat
SE9403597-9 1994-10-20

Publications (1)

Publication Number Publication Date
WO1996012948A1 true WO1996012948A1 (fr) 1996-05-02

Family

ID=20395689

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1995/001229 WO1996012948A1 (fr) 1994-10-20 1995-10-19 Procede et agencement destines a un dispositif d'electrophorese sur gel

Country Status (4)

Country Link
EP (1) EP0787292A1 (fr)
JP (1) JPH10507830A (fr)
SE (1) SE503503C2 (fr)
WO (1) WO1996012948A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001025779A3 (fr) * 1999-10-07 2002-02-28 Europ Lab Molekularbiolog Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987007719A1 (fr) * 1986-06-03 1987-12-17 Europäisches Laboratorium Für Molekularbiologie (E Appareil pour detecter les substances pouvant etre excitees en vue d'une emission photonique
US5100529A (en) * 1990-05-31 1992-03-31 Shimadzu Corporation Fluorescence detection type gel electrophoresis apparatus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987007719A1 (fr) * 1986-06-03 1987-12-17 Europäisches Laboratorium Für Molekularbiologie (E Appareil pour detecter les substances pouvant etre excitees en vue d'une emission photonique
US5100529A (en) * 1990-05-31 1992-03-31 Shimadzu Corporation Fluorescence detection type gel electrophoresis apparatus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001025779A3 (fr) * 1999-10-07 2002-02-28 Europ Lab Molekularbiolog Dispositif d'electrophorese pour l'analyse de molecules marquees, en particulier de molecules biologiques

Also Published As

Publication number Publication date
EP0787292A1 (fr) 1997-08-06
SE503503C2 (sv) 1996-06-24
SE9403597L (sv) 1996-04-21
SE9403597D0 (sv) 1994-10-20
JPH10507830A (ja) 1998-07-28

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