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WO2001068149A2 - Procede et utilisation d'identification biologique d'animaux et de produits animaux - Google Patents

Procede et utilisation d'identification biologique d'animaux et de produits animaux Download PDF

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Publication number
WO2001068149A2
WO2001068149A2 PCT/EP2001/002378 EP0102378W WO0168149A2 WO 2001068149 A2 WO2001068149 A2 WO 2001068149A2 EP 0102378 W EP0102378 W EP 0102378W WO 0168149 A2 WO0168149 A2 WO 0168149A2
Authority
WO
WIPO (PCT)
Prior art keywords
animal
immunogen
animals
changes
products
Prior art date
Application number
PCT/EP2001/002378
Other languages
German (de)
English (en)
Other versions
WO2001068149A3 (fr
Inventor
Johannes Bonenberger
Original Assignee
Denzel, Klaus
Hofmann, Andreas
Rausch, Hans
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Denzel, Klaus, Hofmann, Andreas, Rausch, Hans filed Critical Denzel, Klaus
Priority to EP01911721A priority Critical patent/EP1263476A2/fr
Priority to AU2001240669A priority patent/AU2001240669A1/en
Publication of WO2001068149A2 publication Critical patent/WO2001068149A2/fr
Publication of WO2001068149A3 publication Critical patent/WO2001068149A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
    • A22B—SLAUGHTERING
    • A22B5/00—Accessories for use during or after slaughtering
    • A22B5/20—Splitting instruments
    • A22B5/201—Removing the spinal cord or other nervous tissues
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K11/00—Marking of animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K29/00—Other apparatus for animal husbandry
    • A—HUMAN NECESSITIES
    • A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
    • A22B—SLAUGHTERING
    • A22B5/00—Accessories for use during or after slaughtering
    • A22B5/0064—Accessories for use during or after slaughtering for classifying or grading carcasses; for measuring back fat

Definitions

  • the invention relates to a method and a use for the biological identification of animals, animal products and animal products according to the preamble of claim 1 and from other independent patent claims.
  • Methods of this type are known from DE 196 43 682 C1 and WO99 / 36 775.
  • the methods serve to prove the origin of farm animals, in particular cattle, pigs and the like, and comprise the following steps: a) biological marking of the live farm animal by application of at least one antigen which is harmless to the farm animal and humans, and b) identification of the antigen by the application of the at least one antigen specific antibody formed by an enzyme immunological or immunochemical detection method.
  • the animals are provided with ear tags according to the current legal regulations for labeling. Since 01.01.1998, EU-wide regulations for labeling and registration have been in effect for cattle, which in Germany are still supported by the Beef Labeling Act. These regulations stipulate the labeling of cattle with two ear tags and the creation of accompanying documents from which the origin of the animal can be derived. This measure only applies as long as the ears are on the animal or carcass and the accompanying documents are not manipulated. Before slaughter, the animals are grouped according to their origin and the carcasses are then stamped with origin-specific stamps. After dismantling, carcasses can be mixed and no longer clearly assigned.
  • the first possible procedure is based on individual-specific sequence variants of the deoxyribonucleic acid (DNA) of each animal.
  • a piece of tissue is removed from the animals, for example when attaching the ear tag, which serves as a DNA marker.
  • a genetic fingerprint of this tissue sample can be created via sequence variants of repetitive elements (microsatellite marking) or via single nucleotide polymorphisms (SNP pattern) of the DNA and stored in a database.
  • SNP pattern single nucleotide polymorphisms
  • each piece of beef can be individually assigned due to its genetic uniqueness.
  • DNA labeling requires expensive analysis of the genome of each animal and consistent tracking of the CV of each individual cattle in the database. In addition to the costs of sampling, there are definitely costs for genome mapping and the recording and tracking of the résumé in the database, even if no carcass control is necessary after slaughter.
  • the second possible procedure is based on the principle of immunological labeling, which is comparable to conventional vaccinations.
  • the livestock are given synthetic substances against which the animals form antibodies that can be detected after slaughtering and cutting, for example in meat drip juice.
  • an individual or origin-specific marking should be possible.
  • This method has the advantage over DNA labeling that not every animal in a herd has to be labeled individually.
  • an inventory marker is possible in which each individual in the inventory has the same marker. This reduces the amount of data required enormously, since a stock can always be given the same marking as it grows.
  • the disadvantage of this form of immunological labeling is that each animal must be vaccinated individually by an appropriately trained specialist.
  • This vaccination can be given by injections or via the nasal mucous membrane using a nasal spray or via an implant. This is time-consuming and costly and is currently only acceptable for cattle, since the profit margin for a slaughter animal does not allow any further costs during rearing for smaller animals, even pigs.
  • the object of the invention is to improve the principle of immunological labeling in such a way that labeling individuals and populations of all animal species capable of producing antibodies becomes technically and economically practicable.
  • the invention is intended to make it possible to determine the origin of animal species that cannot be detected with the prior art.
  • This object is achieved according to the invention by a method for the unique biological identification of animals, animal products and animal products in that a) that the living animal at least one defined immunogen, which is harmless to the animal, humans and the environment, orally b) that changes produced by the immunogen in the animal, such as. B. against the immunogen directed receptor structures or against the immunogen formed specific antibodies are detected with a responsive to these changes specific detection method, and c) that the location of the origin of the animal, the animal product or the animal product is clearly demonstrated using the detected changes via a predetermined allocation key.
  • the above-mentioned object is achieved by a method for the unique biological labeling of animals, animal products and animal products in that at least one defined immunogen which is harmless to the animal, humans and the environment is administered to the living animal by oral route, and that an immunogen is used as an immunogen which does not occur in the food chain and in the environment at the animal's place of life.
  • the stated object is achieved according to the invention by the use of at least one immunogen which is harmless to humans and animals for the biological identification of animals, animal products and animal products by oral route on the living animal and by the use for immunological detection of the animal by the immunogen generated changes and for detection of the origin of the animal, the animal product or the animal product according to at least one of claims 1-17.
  • the invention also includes a method for determining the origin of animals to which at least one defined immunogen has been administered, and of animal products and animal products from such animals, characterized in that changes which have been produced in the animal by the at least one immunogen supplied to it, such as B. against the immunogen directed receptor structures or against the immunogen formed specific antibodies, are detected with a responsive to these changes specific detection method, and that the origin of the animal, the animal product, or the animal product is clearly demonstrated using the detected changes via a predetermined allocation key
  • hapten coupled to a suitable carrier, an antigen, a DNA vaccine, a modified protein, a DNA expression vector, a synthetic vector, a hybrid vaccine, a combination of different immunogens, a mucosal adjuvant can be added
  • Immunogen can be presented together with a stopper that counteracts immunogen degradation
  • the at least one immunogen is immunologically added to the feed and / or the drinking water added marking animal so that the animal ingests the at least one immunogen orally in this way.
  • All substances which do not occur in the food chain and in the environment at the animal's place of life and are therefore suitable for associating animals labeled with such a place of origin or place of life can be used as the immunogen.
  • This place of origin or place of life can be the place of birth of the animal, but it can also be a later place of life of the animal, for example where it is believed to be fattening.
  • the animal can be marked both at the place of origin and at the fattening site (where it is fattened) by ingestion of one or more immunogens. In this case, each of these locations can later be verified when the animal changes location or in animal products or animal products. In this way, a large number of individual animals can be marked differently. However, it is also possible to mark entire herds or certain animal species with the same marking.
  • Vaccines which are usually used for therapeutic treatment can also be used as the immunogen, provided that such vaccines are normally not used for the animals to be labeled by an immunogen.
  • a vaccine could be used to label cattle as an immunogen, which is commonly used to vaccinate foxes against rabies, since such a vaccine is not normally used to vaccinate cattle.
  • the rabies vaccine for foxes is an immunogen that is not found "in the food chain and in the environment at the place of life", for example of cattle, pigs and poultry. It is thus an example of what is meant in the present description by the term "in the food chain and in the environment at the animal's place of life", to which the immunogen for origin marking is supplied by oral route.
  • a great advantage of the invention is that not only those substances which have a therapeutic effect are suitable as immunogens, but also "waste products" from research which cause a reaction in the immune system of animals, but no therapeutic effect, for example do not create protection against a particular disease.
  • a single immunogen or several immunogens of different types can be used, either in combination or one after the other to mark the animals at different ages or at different places of life.
  • Oral immunization can significantly reduce the costs of the labeling process if suitable immunogens, individually or in defined combinations, are added to the feed or drinking water.
  • Labeling is inextricably linked to the labeled animal and the products derived from it by this method, as in the injection of the immunogen.
  • Location, date and an animal definition etc. are stored in a database or in some other way, so that this data can be used later to identify the animal's origin or species or to identify special living conditions of the marked animals, if later on the living or dead animal , on whose products or products the "changes" of the animal "generated” by the specific immunogen or the specific immunogens are detected.
  • an "assignment key" which e.g. a comparison table e.g. can be in an electronic database or on another data carrier, the "generated changes" can be clearly assigned to a defined immunogen and from this to the marked animal or the marked animal species and the latter can be recognized.
  • poorly digestible antigens preferably simple molecules and conjugated vaccines, or DNA vaccines are preferably used, which do not occur in the food chain or generally in the environment at the place of origin of the animals and which are also not used as therapeutic vaccines for the animal to be identified.
  • the design feature of the immunogens is to overcome all physiological and immunological barriers after oral intake in order to form the specific antibodies, which can be detected using an enzyme-immuno or immunochemical method. Modified proteins or haptens coupled to suitable carriers are used as immunogens, which are harmless to the animal and harmless to human consumption.
  • synthetic or natural polymers for example naturally non-occurring or non-locally occurring enantiomers of natural products, are also suitable as haptens.
  • Recombinant or attenuated bacteria and viruses in connection with mucosal adjuvants or protease-resistant bacterial toxins can be used as the haptic carrier.
  • DNA expression vectors and synthetic vectors for example proteoliposomes and lipoproteids, are also suitable as immunogens. All these foreign and harmless molecules for the animal organism enable according to the invention individually or in Combination a unique biological labeling of animals, animal products and animal products.
  • the use of a mixture of different immunogens is advantageous in order to increase the number of labeling options, and the antigenic mixture can also be administered on a single hybrid vaccine.
  • Certain immunogens can stand for the identification of a region or a certain animal species.
  • the further combination of immunogens allows the pooling of animal populations.
  • An advantage of oral immunization by ingestion of the immunogens with the feed or drinking water is that the antibodies formed, although desirable, but not necessarily, have to remain permanently in the body of the immunized animal, since booster immunizations can be repeated periodically at low cost. This ensures that a uniformly high titer of immunogen-specific antibodies is present after the immune response has responded.
  • the age of the animals to be immunized plays a subordinate role due to a periodic refreshment.
  • the species-specific immunization cycle is more important.
  • the species-specific and / or posture-specific cycle length ensures that each animal comes into contact with the immunogen or immunogens at least once in the most sensitive period of its type for immunization and can form the corresponding antibodies.
  • the identification of the antibodies or receptor structures specific for the labeling can be carried out using various immunological detection methods.
  • the following highly sensitive immunoassays Enzyme Immuno Assay (EIA), Enzyme Linked Immunosorbent Assay (ELISA), Fluorescent Immuno Assay (FIA), Radio Immuno Assay (RIA), Luminescent Immuno Assay (LIA) etc.
  • EIA Enzyme Immuno Assay
  • ELISA Enzyme Linked Immunosorbent Assay
  • FIA Fluorescent Immuno Assay
  • RIA Radio Immuno Assay
  • LIA Luminescent Immuno Assay
  • the identification of the changes produced by the immunogen in the animal can also be carried out by molecular biological detection methods, e.g. B. Southern blot, Western blot, PCR (polymerase chain reaction), or biochemical detection methods, e.g. B. Use of enzyme-catalyzed reactions.
  • molecular biological detection methods e.g. B. Southern blot, Western blot, PCR (polymerase chain reaction), or biochemical detection methods, e.g. B. Use of enzyme-catalyzed reactions.
  • the number of possible combinations for the unique labeling of individual animals, individual animal species or an animal population depends on the number of immunogens used for the immunization and the total number of immunogens available for the combination.
  • the invention thus enables the immunological labeling of animals of any kind. Not only individual animals as individuals, but also various animal species or certain animal populations, in particular livestock populations, are suitable for immunological labeling. Vertebrates, which are capable of forming antibodies, are particularly suitable for labeling. especially mammals, but also birds, lizards, fish and amphibians
  • the labeling is preferably carried out by adding the at least one immunogen to the animal feed or drinking water, preferably in the form of artificial antigens or combinations of antigens. which are harmless to animals, humans and the environment and which cause the formation in the animal of receptor structures or specific antibodies directed against the immunogen.
  • the receptor structures or antibodies produced in response to an immunogen in the animal are later not only in the live animal, but also in the meat and meat juice, in the saliva and blood of slaughtered animals, as well as in the milk and eggs and other animal products of the labeled Animals present and detectable, in particular detectable by an immunological method.
  • the invention also relates to a method for determining the origin of animals to which at least one defined immunogen has been administered and of animal products and animal products from such animals, characterized in that changes in the animal by the at least one of them, preferably by the oral route , supplied immunogen were generated, such as. B. against the immunogen directed receptor structures or against the immunogen formed specific antibodies can be detected with a specific detection method reacting to these changes, and that using the detected changes over a predetermined allocation key the origin of the animal, the animal product, or the animal product clearly is proven.
  • the detection of the changes produced by the immunogen in the animal is preferably carried out by a molecular biological or biochemical or immunological method or a molecular biological or biochemical or immunological rapid test method.

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Birds (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

La présente invention concerne un procédé d'identification biologique d'animaux et de produits animaux, selon lequel au moins un immunogène défini est admis à l'animal vivant, par voie orale, par exemple dans sa nourriture ou dans son eau potable. Les changements ainsi provoqués chez l'animal sont détectés et utilisés, afin de déterminer l'origine de l'animal ou de ses produits, au moyen d'une clé de correspondance.
PCT/EP2001/002378 2000-03-13 2001-03-02 Procede et utilisation d'identification biologique d'animaux et de produits animaux WO2001068149A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01911721A EP1263476A2 (fr) 2000-03-13 2001-03-02 Procede et utilisation d'identification biologique d'animaux et de produits animaux
AU2001240669A AU2001240669A1 (en) 2000-03-13 2001-03-02 Method and use for biologically marking animals and animal products

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10011741.4 2000-03-13
DE2000111741 DE10011741A1 (de) 2000-03-13 2000-03-13 Verfahren und Verwendung zur biologischen Kennzeichnung von Tieren, Tierprodukten und tierischen Erzeugnissen

Publications (2)

Publication Number Publication Date
WO2001068149A2 true WO2001068149A2 (fr) 2001-09-20
WO2001068149A3 WO2001068149A3 (fr) 2002-06-20

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Application Number Title Priority Date Filing Date
PCT/EP2001/002378 WO2001068149A2 (fr) 2000-03-13 2001-03-02 Procede et utilisation d'identification biologique d'animaux et de produits animaux

Country Status (4)

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EP (1) EP1263476A2 (fr)
AU (1) AU2001240669A1 (fr)
DE (1) DE10011741A1 (fr)
WO (1) WO2001068149A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004078926A2 (fr) * 2003-02-28 2004-09-16 Board Of Trustees Operating Michigan State University Marqueurs immunologiques
NL2021254B1 (en) * 2018-07-05 2020-01-15 Stichting Wageningen Res Method and system for grading individual poultry hens in a flock

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776713A (en) * 1988-02-02 1998-07-07 Biocode Ltd. Marking of products to establish identity and source
US5478990A (en) * 1993-10-14 1995-12-26 Coleman Environmental Systems, Inc. Method for tracking the production history of food products
DE19643682C1 (de) * 1996-10-23 1998-01-15 Manfred Prof Dr Gareis Verfahren zum Herkunftsnachweis von Nutztieren sowie davon stammenden Produkten
EP1047940A1 (fr) * 1998-01-14 2000-11-02 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Procede pour detecter l'origine d'animaux d'elevage ainsi que des produits qui en sont derives

Also Published As

Publication number Publication date
DE10011741A1 (de) 2001-09-20
WO2001068149A3 (fr) 2002-06-20
AU2001240669A1 (en) 2001-09-24
EP1263476A2 (fr) 2002-12-11

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