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WO2001064945A3 - Nouvelles puces a adn - Google Patents

Nouvelles puces a adn Download PDF

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Publication number
WO2001064945A3
WO2001064945A3 PCT/FR2001/000604 FR0100604W WO0164945A3 WO 2001064945 A3 WO2001064945 A3 WO 2001064945A3 FR 0100604 W FR0100604 W FR 0100604W WO 0164945 A3 WO0164945 A3 WO 0164945A3
Authority
WO
WIPO (PCT)
Prior art keywords
mutation
dna
chip
absence
nucleic acid
Prior art date
Application number
PCT/FR2001/000604
Other languages
English (en)
Other versions
WO2001064945A2 (fr
Inventor
Fabrice Cailloux
Original Assignee
Nucleica
Fabrice Cailloux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nucleica, Fabrice Cailloux filed Critical Nucleica
Priority to AU2001240743A priority Critical patent/AU2001240743A1/en
Priority to EP01911814A priority patent/EP1259644A2/fr
Priority to JP2001563632A priority patent/JP2003527846A/ja
Priority to CA002401867A priority patent/CA2401867A1/fr
Publication of WO2001064945A2 publication Critical patent/WO2001064945A2/fr
Publication of WO2001064945A3 publication Critical patent/WO2001064945A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention se rapporte à un système de puces à ADN pour détecter une mutation dans une acide nucléique cible de maniéreà ce que seul l'ADN comportant la mutation demeure sur la puce à l'issu du procédé. L'invention concerne un procédé dans lequel un αS-phosphothioatedésoxynucléotide complémentaire de la mutation est ajouté au moyen d'une ADN polymérase à l'extrémité 3' de la sonde hybridée à l'acide nucléique cible et dans lequel une exonucléase est ajoutée de sorte que seules les sondes élongées ne sont pas dégradées. La détection de la présence ou de l'absence de la mutation est effectuée par mesure directe ou indirecte de la présence ou de l'absence d'ADN en un site donné sur la puce. Avantageusement, la puce comprend des transistors du type ISFET ou des transducteurs piézo-électriques.
PCT/FR2001/000604 2000-03-01 2001-03-01 Nouvelles puces a adn WO2001064945A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2001240743A AU2001240743A1 (en) 2000-03-01 2001-03-01 Novel dna chips
EP01911814A EP1259644A2 (fr) 2000-03-01 2001-03-01 Nouvelles puces a adn
JP2001563632A JP2003527846A (ja) 2000-03-01 2001-03-01 新規なｄｎａチップ
CA002401867A CA2401867A1 (fr) 2000-03-01 2001-03-01 Nouvelles puces a adn

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0002614A FR2805826B1 (fr) 2000-03-01 2000-03-01 Nouvelles puces a adn
FR00/02614 2000-03-01

Publications (2)

Publication Number Publication Date
WO2001064945A2 WO2001064945A2 (fr) 2001-09-07
WO2001064945A3 true WO2001064945A3 (fr) 2002-03-28

Family

ID=8847583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2001/000604 WO2001064945A2 (fr) 2000-03-01 2001-03-01 Nouvelles puces a adn

Country Status (7)

Country Link
US (1) US20030186262A1 (fr)
EP (1) EP1259644A2 (fr)
JP (1) JP2003527846A (fr)
AU (1) AU2001240743A1 (fr)
CA (1) CA2401867A1 (fr)
FR (1) FR2805826B1 (fr)
WO (1) WO2001064945A2 (fr)

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FR2835058B1 (fr) * 2002-01-21 2004-03-12 Centre Nat Rech Scient Procede de detection d'au moins un parametre caracteristique de molecules sondes fixees sur au moins une zone active d'un capteur
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JP2004144630A (ja) * 2002-10-25 2004-05-20 Nikon Corp 有機分子検出素子および有機分子検出装置
DE10309349B4 (de) * 2003-03-03 2005-11-10 Micronas Holding Gmbh Vorrichtung zur Untersuchung eines Analyten
WO2004106891A2 (fr) 2003-05-22 2004-12-09 University Of Hawaii Capteur biochimique ultrasensible
US7317216B2 (en) 2003-10-31 2008-01-08 University Of Hawaii Ultrasensitive biochemical sensing platform
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JP4608697B2 (ja) * 2004-08-27 2011-01-12 独立行政法人物質・材料研究機構 電界効果デバイスを用いたｄｎａ塩基配列解析方法及び塩基配列解析装置
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WO2007008246A2 (fr) 2004-11-12 2007-01-18 The Board Of Trustees Of The Leland Stanford Junior University Systeme de detection de perturbation de charge destine a l'adn et autres molecules
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JP4945279B2 (ja) * 2007-03-23 2012-06-06 株式会社日立製作所 Ｄｎａ分析方法および分析装置
CN101743326A (zh) * 2007-05-14 2010-06-16 因赛特遗传学公司 筛选核酸中的单核苷酸变异的方法
JP4731544B2 (ja) * 2007-12-17 2011-07-27 株式会社日立製作所 生体分子検出装置及びそれを用いた生体分子検出方法
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WO1987002066A1 (fr) * 1985-09-26 1987-04-09 The University Of Southern Mississippi Dispositif piezoelectrique pour la detection de l'hybridation de polynucleotides
WO1991005261A1 (fr) * 1989-10-04 1991-04-18 E.I. Du Pont De Nemours And Company Methode d'analyse pour des complexes de cibles biologiques situes a la surface d'un capteur biologique
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Also Published As

Publication number Publication date
JP2003527846A (ja) 2003-09-24
US20030186262A1 (en) 2003-10-02
WO2001064945A2 (fr) 2001-09-07
FR2805826B1 (fr) 2002-09-20
EP1259644A2 (fr) 2002-11-27
CA2401867A1 (fr) 2001-09-07
FR2805826A1 (fr) 2001-09-07
AU2001240743A1 (en) 2001-09-12

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