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WO2000012704A3 - Expression fonctionnelle amelioree des recepteurs couples a la proteine g - Google Patents

Expression fonctionnelle amelioree des recepteurs couples a la proteine g Download PDF

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Publication number
WO2000012704A3
WO2000012704A3 PCT/US1999/020011 US9920011W WO0012704A3 WO 2000012704 A3 WO2000012704 A3 WO 2000012704A3 US 9920011 W US9920011 W US 9920011W WO 0012704 A3 WO0012704 A3 WO 0012704A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
coupled receptors
functional expression
enhanced functional
receptors
Prior art date
Application number
PCT/US1999/020011
Other languages
English (en)
Other versions
WO2000012704A9 (fr
WO2000012704A2 (fr
Inventor
Mark Henry Pausch
Margaret Lai
Sanford Silverman
Camelia Birsan
William Baumbauch
Eugene Tseng
Eileen Marie Kajkowski
Bradley Alton Ozenberger
Original Assignee
Mark Henry Pausch
Margaret Lai
Sanford Silverman
Camelia Birsan
William Baumbauch
Eugene Tseng
Eileen Marie Kajkowski
Bradley Alton Ozenberger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mark Henry Pausch, Margaret Lai, Sanford Silverman, Camelia Birsan, William Baumbauch, Eugene Tseng, Eileen Marie Kajkowski, Bradley Alton Ozenberger filed Critical Mark Henry Pausch
Priority to AU57009/99A priority Critical patent/AU5700999A/en
Priority to EP99944033A priority patent/EP1112359A2/fr
Priority to JP2000567691A priority patent/JP2002523090A/ja
Priority to CA002343046A priority patent/CA2343046A1/fr
Publication of WO2000012704A2 publication Critical patent/WO2000012704A2/fr
Publication of WO2000012704A9 publication Critical patent/WO2000012704A9/fr
Publication of WO2000012704A3 publication Critical patent/WO2000012704A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722—G-proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Neurology (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des récepteurs mutants couplés à la protéine G, lesquels exercent une action constitutive. L'invention concerne également des cellules de levure exprimant ces récepteurs, des vecteurs convenant à la fabrication de ces cellules et des méthodes de fabrication et d'utilisation associées.
PCT/US1999/020011 1998-09-01 1999-09-01 Expression fonctionnelle amelioree des recepteurs couples a la proteine g WO2000012704A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU57009/99A AU5700999A (en) 1998-09-01 1999-09-01 Enhanced functional expression of g protein-coupled receptors
EP99944033A EP1112359A2 (fr) 1998-09-01 1999-09-01 Expression fonctionnelle amelioree des recepteurs couples a la proteine g
JP2000567691A JP2002523090A (ja) 1998-09-01 1999-09-01 異種ｇタンパク質共役受容体の増強された機能的発現
CA002343046A CA2343046A1 (fr) 1998-09-01 1999-09-01 Expression fonctionnelle amelioree des recepteurs couples a la proteine g

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9870498P 1998-09-01 1998-09-01
US60/098,704 1998-09-01

Publications (3)

Publication Number Publication Date
WO2000012704A2 WO2000012704A2 (fr) 2000-03-09
WO2000012704A9 WO2000012704A9 (fr) 2000-06-08
WO2000012704A3 true WO2000012704A3 (fr) 2000-11-30

Family

ID=22270543

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1999/020011 WO2000012704A2 (fr) 1998-09-01 1999-09-01 Expression fonctionnelle amelioree des recepteurs couples a la proteine g
PCT/US1999/020013 WO2000012705A2 (fr) 1998-09-01 1999-09-01 Methodes permettant d'ameliorer la fonction des recepteurs heterologues couples a la proteine g

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US1999/020013 WO2000012705A2 (fr) 1998-09-01 1999-09-01 Methodes permettant d'ameliorer la fonction des recepteurs heterologues couples a la proteine g

Country Status (5)

Country Link
EP (2) EP1112359A2 (fr)
JP (2) JP2002523091A (fr)
AU (2) AU756244B2 (fr)
CA (2) CA2344591A1 (fr)
WO (2) WO2000012704A2 (fr)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6528271B1 (en) 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
US5891646A (en) 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
US7541151B2 (en) 1997-06-05 2009-06-02 Duke University Single-cell biosensor for the measurement of GPCR ligands in a test sample
AU2001255613B2 (en) 2000-04-24 2005-08-04 Wyeth Transgenic animal
IL155595A0 (en) * 2000-10-26 2003-11-23 Univ British Columbia A functional assay for g-protein-coupled receptors based on insect cells
AU2002210982A1 (en) * 2000-11-01 2002-05-15 Yamanouchi Pharmaceutical Co..Ltd. Method of screening antiplatelet
US7163800B2 (en) 2000-11-03 2007-01-16 Molecular Devices Corporation Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
US7018812B2 (en) 2000-11-03 2006-03-28 Duke University Modified G-protein coupled receptors
DE10056899A1 (de) 2000-11-16 2002-05-29 Aventis Pharma Gmbh Promotor zur funktionellen Charakterisierung von G-Protein gekoppelten Rezeptoren in der Hefe Saccharomyces cerevisiae
WO2002045503A2 (fr) * 2000-12-06 2002-06-13 Deltagen, Inc. Souris transgeniques contenant des disruptions geniques ciblees
GB0030038D0 (en) * 2000-12-08 2001-01-24 Univ Warwick Yeast-based assay
US7279324B2 (en) 2001-01-23 2007-10-09 Duke University Nucleic acid encoding G-protein coupled receptor with modified DRY motif
EP1336655A1 (fr) * 2002-01-12 2003-08-20 Aventis Pharma Deutschland GmbH Méthode pour l'identification des mutants ayant une actività constitutive
DE10233516A1 (de) * 2002-07-23 2004-02-12 Aventis Pharma Deutschland Gmbh Verfahren zur Identifizierung von Substanzen
WO2006104254A1 (fr) * 2005-03-29 2006-10-05 Bio-Energy Corporation Procede pour le criblage d'une substance de liaison des recepteurs
EP1871362A2 (fr) 2005-04-27 2008-01-02 Arena Pharmaceuticals, Inc. Polytherapie pour le traitement, d'une part, de l'obesite et de diabetes et des troubles associes et, d'autre part, de troubles ameliores par l'augmentation d'un niveau glp-1 sanguin
CA2613235A1 (fr) 2005-06-30 2007-01-11 Prosidion Limited Agonistes de gpcr
CA2618488C (fr) 2006-04-11 2010-07-13 Arena Pharmaceuticals, Inc. Procedes d'utilisation du recepteur gpr119 pour identifier des composes utiles pour augmenter la masse osseuse chez un individu
ATE523507T1 (de) 2007-01-04 2011-09-15 Prosidion Ltd Piperidin-gpcr-agonisten
CL2008000017A1 (es) 2007-01-04 2008-08-01 Prosidion Ltd Compuestos derivados de heterociclos de nitrogeno y oxigeno, agonistas de gpcr; composicion farmaceutica que comprende a dicho compuesto; y uso del compuesto para el tratamiento de la obesidad, diabetes, sindrome metabolico, hiperlipidemia, toleranci
CL2008000018A1 (es) 2007-01-04 2008-08-01 Prosidion Ltd Compuestos derivados de heterociclos de nitrogeno y oxigeno, agonistas de gpcr; composicion farmaceutica que comprende a dicho compuesto; y uso del compuesto para el tratamiento de la obesidad, diabetes, sindrome metabolico, hiperlipidemia, toleranci
PT2121919E (pt) * 2007-03-22 2012-05-17 Heptares Therapeutics Ltd Receptores acoplados à proteína-g e métodos para a sua selecção
EP2200609A1 (fr) 2007-09-10 2010-06-30 Prosidion Limited Composés pour le traitement de troubles métaboliques
GB0720389D0 (en) 2007-10-18 2008-11-12 Prosidion Ltd G-Protein Coupled Receptor Agonists
GB0720390D0 (en) 2007-10-18 2007-11-28 Prosidion Ltd G-Protein coupled receptor agonists
EP2146210A1 (fr) 2008-04-07 2010-01-20 Arena Pharmaceuticals, Inc. Procédés d'utilisation du récepteur couplé aux protéines A G pour identifier les secrétagogues de peptide YY (PYY) et composés utiles dans le traitement d'états modulés par PYY
GB0904287D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
GB0904285D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
GB0904284D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
JP7422498B2 (ja) * 2019-07-25 2024-01-26 芳武 村山 ペプチド、細胞遊走抑制剤、細胞浸潤抑制剤、がん細胞転移抑制剤、及び医薬

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021925A1 (fr) * 1994-02-14 1995-08-17 American Cyanamid Company Recepteurs heterologues couples a des proteines g et exprimes dans la levure, leur fusion avec des proteines g et leur utilisation dans des dosages biologiques
WO1996000739A1 (fr) * 1994-06-29 1996-01-11 Merck & Co., Inc. Recepteurs modifies lies a des proteines g
WO1998038217A1 (fr) * 1997-02-27 1998-09-03 Milt Teitler Recepteurs de serotonine actives par constitution

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021925A1 (fr) * 1994-02-14 1995-08-17 American Cyanamid Company Recepteurs heterologues couples a des proteines g et exprimes dans la levure, leur fusion avec des proteines g et leur utilisation dans des dosages biologiques
WO1996000739A1 (fr) * 1994-06-29 1996-01-11 Merck & Co., Inc. Recepteurs modifies lies a des proteines g
WO1998038217A1 (fr) * 1997-02-27 1998-09-03 Milt Teitler Recepteurs de serotonine actives par constitution

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
A. SCHEER ET AL: "Constitutively active G-protein-coupled receptors: Potential mechanisms of receptor activation", JOURNAL OF RECEPTOR & SIGNAL TRANSDUCTION RESEARCH, vol. 17, no. 1-3, 1997, pages 57 - 73, XP000867531 *
EGGERICKX D ET AL: "MOLECULAR CLONING OF AN ORPHAN G-PROTEIN-COUPLED RECEPTOR THAT CONSTITUTIVELY ACTIVATES ADENYLATE CYCLASE", BIOCHEMICAL JOURNAL,GB,PORTLAND PRESS, LONDON, vol. 309, 1995, pages 837 - 843, XP002912497, ISSN: 0264-6021 *
M.A. KJELSBERG ET AL: "Constitutive activation of the alpha1B-adrenergic receptor by all amino acid substitutions at a single site", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 267, no. 3, 25 January 1992 (1992-01-25), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 1430 - 1433, XP002135768, ISSN: 0021-9258 *
P. SAMAMA ET AL: "A mutation-induced activated state of the beta2-adrenergic receptor", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 268, no. 7, 5 March 1993 (1993-03-05), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 4625 - 4636, XP002135769, ISSN: 0021-9258 *
PAUSCH M H ET AL: "HETEROLOGOUS G PROTEIN-COUPLED RECEPTORS EXPRESSED IN SACCHAROMYCES CEREVISIAE: METHODS FOR GENETIC ANALYSIS AND LIGAND IDENTIFICATION", IDENTIFICATION AND EXPRESSION OF G-PROTEIN-COUPLED RECEPTORS. CHAPTER II, 26 May 1999 (1999-05-26), Editedby Kevin R. Lynch, Wley-Liss, Inc., pages 196 - 212, XP000869632 *
PAUSCH M H: "G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery", TRENDS IN BIOTECHNOLOGY,GB,ELSEVIER PUBLICATIONS, CAMBRIDGE, vol. 15, no. 12, 1 December 1997 (1997-12-01), pages 487 - 494, XP004097426, ISSN: 0167-7799 *
PAUWELS P J ET AL: "REVIEW:AMINO ACID DOMAINS INVOLVED IN CONSTITUTIVE ACTIVATION OF G-PROTEIN-COUPLED RECEPTORS", MOLECULAR NEUROBIOLOGY,US,HUMANA PRESS, vol. 17, no. 1/03, 29 December 1998 (1998-12-29), pages 109 - 135, XP000866477, ISSN: 0893-7648 *

Also Published As

Publication number Publication date
WO2000012705A2 (fr) 2000-03-09
CA2343046A1 (fr) 2000-03-09
AU5700999A (en) 2000-03-21
CA2344591A1 (fr) 2000-03-09
JP2002523090A (ja) 2002-07-30
WO2000012704A9 (fr) 2000-06-08
AU756244B2 (en) 2003-01-09
AU5701199A (en) 2000-03-21
WO2000012705A9 (fr) 2000-07-13
JP2002523091A (ja) 2002-07-30
EP1123391A2 (fr) 2001-08-16
WO2000012705A3 (fr) 2000-10-05
EP1112359A2 (fr) 2001-07-04
WO2000012704A2 (fr) 2000-03-09

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